Heterobasidion partitivirus 3
Average proteome isoelectric point is 7.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C1KDH7|C1KDH7_9VIRU Putative coat protein OS=Heterobasidion partitivirus 3 OX=631431 PE=4 SV=1
MM1 pKa = 7.8 SSIPDD6 pKa = 3.65 FASMNEE12 pKa = 3.94 SDD14 pKa = 3.17 RR15 pKa = 11.84 LKK17 pKa = 11.02 YY18 pKa = 10.55 FEE20 pKa = 4.5 EE21 pKa = 4.04 QQKK24 pKa = 9.55 KK25 pKa = 7.92 QKK27 pKa = 10.51 EE28 pKa = 4.25 LIDD31 pKa = 3.55 QSIKK35 pKa = 10.82 SKK37 pKa = 8.81 QTMSAMRR44 pKa = 11.84 VRR46 pKa = 11.84 PVFQQARR53 pKa = 11.84 NNVTSVNTSSSVPTKK68 pKa = 10.45 AVPPQPFSGTSSSANNMLRR87 pKa = 11.84 LAFGEE92 pKa = 4.12 NPFRR96 pKa = 11.84 PKK98 pKa = 10.41 QRR100 pKa = 11.84 FGQNGFVPDD109 pKa = 4.01 SQHH112 pKa = 6.78 LFALLSVMDD121 pKa = 4.77 AKK123 pKa = 10.3 MASTRR128 pKa = 11.84 KK129 pKa = 9.17 FVEE132 pKa = 4.78 GCEE135 pKa = 3.8 FWTPLVSQYY144 pKa = 10.91 YY145 pKa = 9.98 LSVLIFIQVARR156 pKa = 11.84 AKK158 pKa = 10.55 HH159 pKa = 4.69 EE160 pKa = 4.57 AGLLSGEE167 pKa = 4.23 LADD170 pKa = 5.78 VYY172 pKa = 11.59 DD173 pKa = 4.85 LFCGHH178 pKa = 6.37 SPAINLNALPVPGPFLNMLSQVAAHH203 pKa = 6.81 IPHH206 pKa = 6.91 LVDD209 pKa = 3.99 MDD211 pKa = 4.79 NICPIVPNNLEE222 pKa = 4.07 ATNTSYY228 pKa = 11.68 YY229 pKa = 10.41 LYY231 pKa = 10.32 TGQCQNLRR239 pKa = 11.84 GRR241 pKa = 11.84 LPNVPYY247 pKa = 10.47 ILDD250 pKa = 3.48 QLHH253 pKa = 5.58 QLGVLLTANPVGIDD267 pKa = 3.21 VANQGRR273 pKa = 11.84 VLHH276 pKa = 6.46 HH277 pKa = 6.78 SFLGVPMFDD286 pKa = 2.93 ATVAPGNAAQWNSRR300 pKa = 11.84 VAFDD304 pKa = 4.79 AGFTASGSFIAVDD317 pKa = 3.61 PAARR321 pKa = 11.84 HH322 pKa = 5.48 PFFLNRR328 pKa = 11.84 GLLSQITDD336 pKa = 3.41 YY337 pKa = 11.53 AALINVTRR345 pKa = 11.84 PARR348 pKa = 11.84 QNAAAVTPSWTQFLGLDD365 pKa = 3.44 NMPFFLQVVRR375 pKa = 11.84 IMSWYY380 pKa = 9.16 SKK382 pKa = 9.36 FWQGSSDD389 pKa = 3.6 LMSFTPSGHH398 pKa = 6.39 TGGQNTFEE406 pKa = 4.6 LVNPPASLNAMTAGGRR422 pKa = 11.84 FYY424 pKa = 10.85 TLDD427 pKa = 4.2 LKK429 pKa = 11.42 SKK431 pKa = 9.95 GYY433 pKa = 10.16 SRR435 pKa = 11.84 NPLAPDD441 pKa = 4.14 ADD443 pKa = 4.23 CFDD446 pKa = 4.39 AALGPINLSWTPADD460 pKa = 3.51 MFAGKK465 pKa = 9.77 LARR468 pKa = 11.84 NGAIADD474 pKa = 3.88 EE475 pKa = 4.6 TTLSNTRR482 pKa = 11.84 TAGPYY487 pKa = 9.48 FSEE490 pKa = 4.69 TIVDD494 pKa = 3.29 ITGEE498 pKa = 4.17 INPSSAYY505 pKa = 10.34 AGILNQYY512 pKa = 8.75 YY513 pKa = 9.59 YY514 pKa = 11.47 SSVALKK520 pKa = 10.59 NN521 pKa = 3.48
Molecular weight: 57.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.569
IPC2_protein 6.576
IPC_protein 6.766
Toseland 6.839
ProMoST 7.132
Dawson 7.263
Bjellqvist 7.146
Wikipedia 7.234
Rodwell 7.249
Grimsley 6.956
Solomon 7.307
Lehninger 7.307
Nozaki 7.468
DTASelect 7.541
Thurlkill 7.571
EMBOSS 7.614
Sillero 7.658
Patrickios 4.088
IPC_peptide 7.307
IPC2_peptide 7.146
IPC2.peptide.svr19 7.045
Protein with the highest isoelectric point:
>tr|C1KDH7|C1KDH7_9VIRU Putative coat protein OS=Heterobasidion partitivirus 3 OX=631431 PE=4 SV=1
MM1 pKa = 7.69 KK2 pKa = 10.14 IFSTLYY8 pKa = 10.31 SSFASFAKK16 pKa = 8.47 WTGLTDD22 pKa = 3.21 PHH24 pKa = 8.0 GFEE27 pKa = 4.62 HH28 pKa = 6.69 NFYY31 pKa = 9.6 FTGYY35 pKa = 9.61 ADD37 pKa = 3.78 KK38 pKa = 10.63 KK39 pKa = 10.02 IKK41 pKa = 10.7 VSINPRR47 pKa = 11.84 FQEE50 pKa = 4.23 VYY52 pKa = 10.76 DD53 pKa = 4.76 DD54 pKa = 3.76 YY55 pKa = 11.66 QSYY58 pKa = 10.33 VGKK61 pKa = 10.41 FIDD64 pKa = 3.37 KK65 pKa = 10.25 HH66 pKa = 7.59 LPGHH70 pKa = 5.92 MAQKK74 pKa = 10.32 IKK76 pKa = 10.51 FGYY79 pKa = 7.95 HH80 pKa = 5.87 HH81 pKa = 7.42 PVASLPFMITNLKK94 pKa = 10.55 KK95 pKa = 10.7 GDD97 pKa = 4.16 LPDD100 pKa = 4.13 HH101 pKa = 6.57 PVPHH105 pKa = 6.86 DD106 pKa = 3.4 QHH108 pKa = 5.34 YY109 pKa = 7.47 TAARR113 pKa = 11.84 KK114 pKa = 8.69 AAADD118 pKa = 3.4 AFRR121 pKa = 11.84 PPRR124 pKa = 11.84 LVRR127 pKa = 11.84 PVHH130 pKa = 5.99 FADD133 pKa = 3.66 LRR135 pKa = 11.84 YY136 pKa = 10.32 YY137 pKa = 9.43 KK138 pKa = 10.13 WNWHH142 pKa = 6.31 PNVEE146 pKa = 4.18 EE147 pKa = 4.93 PYY149 pKa = 10.86 YY150 pKa = 10.76 SDD152 pKa = 4.85 PKK154 pKa = 8.47 LQQYY158 pKa = 9.07 VEE160 pKa = 3.99 HH161 pKa = 7.34 CYY163 pKa = 11.07 ALGLIDD169 pKa = 5.33 DD170 pKa = 4.7 ARR172 pKa = 11.84 LSFGNLKK179 pKa = 10.76 DD180 pKa = 3.91 FVFMDD185 pKa = 3.51 TRR187 pKa = 11.84 HH188 pKa = 5.57 YY189 pKa = 11.06 LHH191 pKa = 7.36 LIKK194 pKa = 10.87 NGSITDD200 pKa = 3.89 NNQLWPIMKK209 pKa = 9.55 IHH211 pKa = 6.55 VKK213 pKa = 9.79 PALTEE218 pKa = 3.8 PTEE221 pKa = 4.01 TKK223 pKa = 9.93 IRR225 pKa = 11.84 VIYY228 pKa = 9.95 GVSKK232 pKa = 10.58 RR233 pKa = 11.84 HH234 pKa = 6.15 ILAQAMFFWPLFRR247 pKa = 11.84 YY248 pKa = 9.91 YY249 pKa = 9.75 IEE251 pKa = 4.36 EE252 pKa = 4.16 HH253 pKa = 6.1 TSPLLWGNEE262 pKa = 3.87 TFTGGMLKK270 pKa = 9.36 IHH272 pKa = 6.27 NLISVPRR279 pKa = 11.84 LYY281 pKa = 10.81 SQTYY285 pKa = 8.02 LTVDD289 pKa = 3.2 WSGFDD294 pKa = 3.54 LRR296 pKa = 11.84 SLFTIQRR303 pKa = 11.84 EE304 pKa = 4.15 IFDD307 pKa = 3.37 DD308 pKa = 2.94 WRR310 pKa = 11.84 TYY312 pKa = 11.32 FDD314 pKa = 3.47 FTAYY318 pKa = 9.66 IPTRR322 pKa = 11.84 TYY324 pKa = 10.71 PDD326 pKa = 3.51 SKK328 pKa = 9.65 TDD330 pKa = 4.41 PIRR333 pKa = 11.84 MEE335 pKa = 5.12 RR336 pKa = 11.84 LWNWQRR342 pKa = 11.84 DD343 pKa = 3.86 ACFKK347 pKa = 10.6 MPFVLPDD354 pKa = 3.04 RR355 pKa = 11.84 TTYY358 pKa = 11.13 ARR360 pKa = 11.84 LFRR363 pKa = 11.84 SIPSGLFVTQFLDD376 pKa = 3.27 SHH378 pKa = 6.99 YY379 pKa = 9.9 NLIMIYY385 pKa = 9.88 TILSAMGFDD394 pKa = 3.64 ITNLMILVQGDD405 pKa = 3.69 DD406 pKa = 3.75 SLIHH410 pKa = 6.62 LKK412 pKa = 10.59 FFLPADD418 pKa = 3.26 QHH420 pKa = 6.72 DD421 pKa = 4.03 AFKK424 pKa = 11.49 AEE426 pKa = 4.15 FEE428 pKa = 4.29 RR429 pKa = 11.84 LAKK432 pKa = 10.7 YY433 pKa = 10.63 YY434 pKa = 9.68 FDD436 pKa = 5.56 HH437 pKa = 7.21 IARR440 pKa = 11.84 PEE442 pKa = 4.02 KK443 pKa = 9.8 THH445 pKa = 5.29 VTNSPNEE452 pKa = 4.07 VEE454 pKa = 4.28 VLGYY458 pKa = 9.19 TNNNGYY464 pKa = 9.58 PSRR467 pKa = 11.84 DD468 pKa = 3.2 MTKK471 pKa = 10.55 LVAQLYY477 pKa = 9.88 HH478 pKa = 6.62 PRR480 pKa = 11.84 NVDD483 pKa = 3.01 KK484 pKa = 10.98 TSWKK488 pKa = 9.47 SLLMAKK494 pKa = 9.82 VCGFAYY500 pKa = 10.29 ASCYY504 pKa = 9.75 QDD506 pKa = 3.06 SQVIDD511 pKa = 4.18 LLRR514 pKa = 11.84 SIYY517 pKa = 10.99 NNLASKK523 pKa = 10.39 GFKK526 pKa = 9.49 PKK528 pKa = 9.97 SGRR531 pKa = 11.84 VMRR534 pKa = 11.84 DD535 pKa = 2.66 IILFGEE541 pKa = 4.59 SEE543 pKa = 4.55 FEE545 pKa = 4.17 VPTDD549 pKa = 4.18 HH550 pKa = 7.52 FPTLNDD556 pKa = 2.85 VTKK559 pKa = 10.09 YY560 pKa = 9.69 FRR562 pKa = 11.84 RR563 pKa = 11.84 PYY565 pKa = 10.82 VRR567 pKa = 11.84 TQRR570 pKa = 11.84 DD571 pKa = 3.12 ADD573 pKa = 3.77 SYY575 pKa = 10.3 FPSWHH580 pKa = 6.42 FNDD583 pKa = 3.7 VFF585 pKa = 4.39
Molecular weight: 68.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.932
IPC2_protein 8.097
IPC_protein 8.024
Toseland 8.331
ProMoST 8.434
Dawson 8.77
Bjellqvist 8.726
Wikipedia 8.916
Rodwell 8.829
Grimsley 8.668
Solomon 8.858
Lehninger 8.799
Nozaki 8.682
DTASelect 8.639
Thurlkill 8.682
EMBOSS 8.887
Sillero 8.873
Patrickios 4.101
IPC_peptide 8.843
IPC2_peptide 7.541
IPC2.peptide.svr19 7.646
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1106
521
585
553.0
62.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.595 ± 1.685
0.814 ± 0.103
5.967 ± 0.961
3.074 ± 0.273
6.781 ± 0.587
5.154 ± 0.834
3.255 ± 0.944
4.882 ± 0.602
4.611 ± 1.088
8.951 ± 0.321
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.712 ± 0.118
5.335 ± 0.977
6.51 ± 0.282
4.069 ± 0.787
4.973 ± 0.53
7.233 ± 1.127
5.877 ± 0.22
5.696 ± 0.451
1.627 ± 0.336
4.882 ± 1.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here