Aeromonas phage AsFcp_2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; unclassified Tevenvirinae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 303 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411BAJ8|A0A411BAJ8_9CAUD Uncharacterized protein OS=Aeromonas phage AsFcp_2 OX=2419736 GN=ASfcp2_312 PE=4 SV=1
MM1 pKa = 7.29TKK3 pKa = 10.24EE4 pKa = 3.47ITQVIIYY11 pKa = 9.77SYY13 pKa = 10.55EE14 pKa = 3.82AVVDD18 pKa = 3.64QDD20 pKa = 4.47EE21 pKa = 4.26NDD23 pKa = 3.45EE24 pKa = 4.5DD25 pKa = 4.12VLEE28 pKa = 3.95EE29 pKa = 4.23HH30 pKa = 6.82YY31 pKa = 10.86GVKK34 pKa = 9.5ATGAAEE40 pKa = 4.28DD41 pKa = 3.96VFGQEE46 pKa = 3.49IFEE49 pKa = 4.69DD50 pKa = 4.87FEE52 pKa = 6.32DD53 pKa = 4.07SLVDD57 pKa = 3.4AHH59 pKa = 6.29EE60 pKa = 4.43TAQQIADD67 pKa = 3.57EE68 pKa = 4.65CGIEE72 pKa = 4.2ITWEE76 pKa = 4.16CYY78 pKa = 9.66KK79 pKa = 10.77PGWAKK84 pKa = 10.87

Molecular weight: 9.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411BAG6|A0A411BAG6_9CAUD Glycine radical domain-containing protein OS=Aeromonas phage AsFcp_2 OX=2419736 GN=ASfcp2_282 PE=4 SV=1
MM1 pKa = 7.6NYY3 pKa = 10.45LKK5 pKa = 10.76RR6 pKa = 11.84LLNRR10 pKa = 11.84MKK12 pKa = 9.73YY13 pKa = 10.57KK14 pKa = 10.72PMIKK18 pKa = 9.64INVYY22 pKa = 10.66SMQIHH27 pKa = 5.75TASGASVCTPDD38 pKa = 5.47GIAIKK43 pKa = 10.43QRR45 pKa = 11.84IEE47 pKa = 3.66RR48 pKa = 11.84LKK50 pKa = 10.94RR51 pKa = 11.84EE52 pKa = 3.65MRR54 pKa = 11.84MNKK57 pKa = 9.7

Molecular weight: 6.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

303

0

303

62767

33

1318

207.2

23.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.838 ± 0.168

1.23 ± 0.064

6.457 ± 0.132

6.76 ± 0.175

4.345 ± 0.099

6.36 ± 0.196

1.905 ± 0.066

6.695 ± 0.106

7.698 ± 0.194

7.053 ± 0.136

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.198 ± 0.108

5.565 ± 0.141

3.033 ± 0.079

3.451 ± 0.093

4.923 ± 0.088

5.978 ± 0.107

5.724 ± 0.205

7.362 ± 0.133

1.273 ± 0.054

4.152 ± 0.108
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski