Aneurinibacillus soli
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3962 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U4WKJ6|A0A0U4WKJ6_9BACL Putative prophage phiRv2 integrase OS=Aneurinibacillus soli OX=1500254 GN=CB4_03179 PE=4 SV=1
MM1 pKa = 7.01 KK2 pKa = 9.93 TYY4 pKa = 10.71 VDD6 pKa = 4.44 KK7 pKa = 10.15 DD8 pKa = 3.43 TCIACGACGATAPDD22 pKa = 3.44 VFDD25 pKa = 4.87 YY26 pKa = 11.17 DD27 pKa = 4.98 DD28 pKa = 4.59 EE29 pKa = 5.6 GLAFNKK35 pKa = 9.8 IDD37 pKa = 4.4 DD38 pKa = 4.29 NANTAEE44 pKa = 4.37 IPDD47 pKa = 3.92 VLHH50 pKa = 7.71 DD51 pKa = 4.37 DD52 pKa = 3.67 VRR54 pKa = 11.84 DD55 pKa = 3.66 AAEE58 pKa = 4.44 GCPTDD63 pKa = 3.83 SIKK66 pKa = 10.82 VEE68 pKa = 4.13 EE69 pKa = 4.22
Molecular weight: 7.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.732
IPC_protein 3.706
Toseland 3.49
ProMoST 3.872
Dawson 3.719
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.541
Grimsley 3.401
Solomon 3.706
Lehninger 3.656
Nozaki 3.846
DTASelect 4.113
Thurlkill 3.567
EMBOSS 3.694
Sillero 3.834
Patrickios 1.875
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.79
Protein with the highest isoelectric point:
>tr|A0A0U5B795|A0A0U5B795_9BACL Uncharacterized protein OS=Aneurinibacillus soli OX=1500254 GN=CB4_03466 PE=4 SV=1
MM1 pKa = 7.34 NLLSCFYY8 pKa = 10.93 FGFCKK13 pKa = 9.41 STRR16 pKa = 11.84 RR17 pKa = 11.84 SGGGRR22 pKa = 11.84 EE23 pKa = 3.85 EE24 pKa = 4.1 RR25 pKa = 11.84 SRR27 pKa = 11.84 SLRR30 pKa = 11.84 YY31 pKa = 8.81 AWQGRR36 pKa = 11.84 VDD38 pKa = 4.86 CPLQEE43 pKa = 4.78 PGEE46 pKa = 4.3 LACKK50 pKa = 10.28 RR51 pKa = 11.84 GPTMLFHH58 pKa = 6.98 RR59 pKa = 11.84 RR60 pKa = 11.84 VAGKK64 pKa = 10.41 SPFAGLLPLGNKK76 pKa = 8.0 RR77 pKa = 11.84 TGALLLLSSLLPTMFRR93 pKa = 11.84 FKK95 pKa = 10.81 KK96 pKa = 9.24 EE97 pKa = 3.86 QNNLWGEE104 pKa = 4.74 AIQDD108 pKa = 3.17 VRR110 pKa = 11.84 LIVICPNLVRR120 pKa = 11.84 SEE122 pKa = 3.6 IRR124 pKa = 11.84 RR125 pKa = 11.84 GNGTPVRR132 pKa = 11.84 ATQRR136 pKa = 11.84 KK137 pKa = 8.16 GAGEE141 pKa = 4.01 QILPTMLLLKK151 pKa = 10.28 YY152 pKa = 10.3 IEE154 pKa = 5.58 GFLLWAVRR162 pKa = 11.84 LAPGADD168 pKa = 3.14 SLNSRR173 pKa = 11.84 EE174 pKa = 4.44 SVPKK178 pKa = 10.55
Molecular weight: 19.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.604
IPC_protein 10.394
Toseland 10.774
ProMoST 10.526
Dawson 10.847
Bjellqvist 10.57
Wikipedia 11.052
Rodwell 11.023
Grimsley 10.877
Solomon 10.994
Lehninger 10.95
Nozaki 10.774
DTASelect 10.555
Thurlkill 10.76
EMBOSS 11.184
Sillero 10.789
Patrickios 10.76
IPC_peptide 10.994
IPC2_peptide 9.78
IPC2.peptide.svr19 8.64
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3962
0
3962
1158177
29
2902
292.3
32.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.235 ± 0.049
0.867 ± 0.012
4.943 ± 0.028
7.08 ± 0.049
3.96 ± 0.028
7.143 ± 0.037
2.186 ± 0.018
6.975 ± 0.035
5.731 ± 0.039
9.554 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.946 ± 0.021
3.721 ± 0.03
3.85 ± 0.026
4.091 ± 0.024
5.136 ± 0.036
5.831 ± 0.034
5.821 ± 0.04
7.543 ± 0.033
1.048 ± 0.014
3.34 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here