Ictalurid herpesvirus 2
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H5AJF0|A0A2H5AJF0_9VIRU ORF11L OS=Ictalurid herpesvirus 2 OX=508441 PE=4 SV=1
MM1 pKa = 7.62 ASNMARR7 pKa = 11.84 AGHH10 pKa = 5.16 VHH12 pKa = 6.3 WCLQLFSEE20 pKa = 5.28 LGPRR24 pKa = 11.84 DD25 pKa = 4.16 DD26 pKa = 5.1 DD27 pKa = 3.92 QSSSSSSSSGSSVSGDD43 pKa = 3.36 TPDD46 pKa = 3.06 WVSTILEE53 pKa = 4.26 TSDD56 pKa = 5.47 DD57 pKa = 4.69 DD58 pKa = 4.71 LSSMPSPVSDD68 pKa = 3.33 RR69 pKa = 11.84 TSSAFSWASDD79 pKa = 3.9 DD80 pKa = 5.0 EE81 pKa = 4.91 EE82 pKa = 6.96 GDD84 pKa = 3.7 DD85 pKa = 5.45 DD86 pKa = 4.85 VFEE89 pKa = 4.52 AAPGGSWSEE98 pKa = 3.75 RR99 pKa = 11.84 TPAEE103 pKa = 4.1 VEE105 pKa = 3.85 VRR107 pKa = 11.84 TPGSSDD113 pKa = 3.87 DD114 pKa = 3.83 EE115 pKa = 4.89 VVCLGVVEE123 pKa = 4.3 KK124 pKa = 11.05 VRR126 pKa = 11.84 LVRR129 pKa = 11.84 EE130 pKa = 4.1 GGVWRR135 pKa = 11.84 VAEE138 pKa = 4.18 RR139 pKa = 11.84 RR140 pKa = 11.84 VEE142 pKa = 4.22 APTPYY147 pKa = 9.92 PWANPEE153 pKa = 3.97 EE154 pKa = 4.33 PTAEE158 pKa = 4.03 DD159 pKa = 4.42 FFTEE163 pKa = 4.45 FAEE166 pKa = 4.48 FDD168 pKa = 3.89 EE169 pKa = 5.2 LDD171 pKa = 3.62 EE172 pKa = 4.43 LAAAIANTPGSPSQYY187 pKa = 10.11 SPPSPDD193 pKa = 3.43 PSHH196 pKa = 7.89 HH197 pKa = 6.32 EE198 pKa = 4.15 DD199 pKa = 3.61 EE200 pKa = 5.04 YY201 pKa = 11.4 EE202 pKa = 4.21 NPPSPTDD209 pKa = 3.58 YY210 pKa = 11.16 SPTSPRR216 pKa = 11.84 RR217 pKa = 11.84 EE218 pKa = 4.23 SLDD221 pKa = 3.48 EE222 pKa = 4.0 EE223 pKa = 4.54 APAYY227 pKa = 10.78 SPITSPEE234 pKa = 3.25 WDD236 pKa = 3.71 YY237 pKa = 10.65 EE238 pKa = 4.31 TRR240 pKa = 11.84 ASPRR244 pKa = 11.84 APEE247 pKa = 3.94 VAEE250 pKa = 4.14 VAEE253 pKa = 4.3 VAPTRR258 pKa = 11.84 GFHH261 pKa = 5.91 MGPLLSDD268 pKa = 3.24 AWVSYY273 pKa = 10.86 EE274 pKa = 4.06 EE275 pKa = 4.18 YY276 pKa = 11.23 ANDD279 pKa = 4.5 FIMDD283 pKa = 3.75 YY284 pKa = 9.29 RR285 pKa = 11.84 TLYY288 pKa = 9.95 HH289 pKa = 5.99 PHH291 pKa = 7.52 RR292 pKa = 11.84 DD293 pKa = 3.6 TPYY296 pKa = 10.68 SPDD299 pKa = 3.02 EE300 pKa = 4.16 RR301 pKa = 11.84 RR302 pKa = 11.84 AGEE305 pKa = 4.39 TPHH308 pKa = 6.81 GPYY311 pKa = 10.47 VPDD314 pKa = 3.87 SPVAGPSRR322 pKa = 11.84 KK323 pKa = 9.26 RR324 pKa = 11.84 GRR326 pKa = 11.84 KK327 pKa = 9.67 GEE329 pKa = 4.3 TKK331 pKa = 10.36 GAPPKK336 pKa = 10.47 KK337 pKa = 9.6 KK338 pKa = 10.43 ALVEE342 pKa = 4.16 GWDD345 pKa = 3.82 EE346 pKa = 3.89 DD347 pKa = 3.92 WEE349 pKa = 5.64 AEE351 pKa = 4.11 LQRR354 pKa = 11.84 IRR356 pKa = 11.84 DD357 pKa = 4.14 GYY359 pKa = 9.66 MGTPITRR366 pKa = 11.84 HH367 pKa = 6.44 DD368 pKa = 4.44 LLPATVEE375 pKa = 3.59 ICPISNCFFSGLKK388 pKa = 10.25 PEE390 pKa = 4.33 INGPTVCKK398 pKa = 10.35 CAICKK403 pKa = 9.91 HH404 pKa = 6.59 DD405 pKa = 4.06 GQCLCLARR413 pKa = 11.84 CARR416 pKa = 11.84 NCPDD420 pKa = 3.81 YY421 pKa = 11.38 EE422 pKa = 4.5 CTCVDD427 pKa = 2.96 IATDD431 pKa = 3.56 NGRR434 pKa = 11.84 DD435 pKa = 3.75 PNPIFDD441 pKa = 5.02 PEE443 pKa = 3.95 DD444 pKa = 3.42 TRR446 pKa = 11.84 NFFIDD451 pKa = 3.16 EE452 pKa = 4.23 CLAKK456 pKa = 10.79 GLTDD460 pKa = 3.9 EE461 pKa = 4.47 VASDD465 pKa = 4.17 LFTAKK470 pKa = 9.45 TLAFQIRR477 pKa = 11.84 NEE479 pKa = 4.04 TRR481 pKa = 11.84 LKK483 pKa = 10.88 EE484 pKa = 3.93 LDD486 pKa = 3.5 NAA488 pKa = 4.47
Molecular weight: 53.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.343
IPC2_protein 4.317
IPC_protein 4.304
Toseland 4.126
ProMoST 4.418
Dawson 4.253
Bjellqvist 4.406
Wikipedia 4.139
Rodwell 4.126
Grimsley 4.024
Solomon 4.253
Lehninger 4.202
Nozaki 4.355
DTASelect 4.546
Thurlkill 4.126
EMBOSS 4.151
Sillero 4.406
Patrickios 3.541
IPC_peptide 4.253
IPC2_peptide 4.406
IPC2.peptide.svr19 4.328
Protein with the highest isoelectric point:
>tr|A0A2H5AJI0|A0A2H5AJI0_9VIRU ORF41 OS=Ictalurid herpesvirus 2 OX=508441 PE=4 SV=1
MM1 pKa = 7.51 TFQFNCWDD9 pKa = 5.16 PILDD13 pKa = 5.22 DD14 pKa = 3.58 ISKK17 pKa = 8.71 TIQIATVDD25 pKa = 4.12 RR26 pKa = 11.84 QPPTYY31 pKa = 9.4 WNYY34 pKa = 8.27 ITAEE38 pKa = 3.88 TGMRR42 pKa = 11.84 GYY44 pKa = 10.84 EE45 pKa = 3.92 LFGYY49 pKa = 9.73 PSLTQSGTIPGSPWRR64 pKa = 11.84 PEE66 pKa = 3.63 MSTLSWPVPATSVHH80 pKa = 6.51 EE81 pKa = 4.09 DD82 pKa = 3.3 VIMAEE87 pKa = 4.32 PKK89 pKa = 10.59 VPIQPRR95 pKa = 11.84 QHH97 pKa = 5.73 RR98 pKa = 11.84 RR99 pKa = 11.84 RR100 pKa = 11.84 RR101 pKa = 11.84 RR102 pKa = 11.84 SRR104 pKa = 11.84 GAGRR108 pKa = 11.84 KK109 pKa = 8.68 SVHH112 pKa = 6.19 ALAPVWSTPSPGTAVRR128 pKa = 11.84 QVPQEE133 pKa = 3.93 QRR135 pKa = 11.84 QQPPTPIKK143 pKa = 9.74 TGSLKK148 pKa = 11.03 SPGAPKK154 pKa = 10.24 SPRR157 pKa = 11.84 VKK159 pKa = 10.38 RR160 pKa = 11.84 GVTFADD166 pKa = 3.21 ACTIVQFDD174 pKa = 4.82 RR175 pKa = 11.84 EE176 pKa = 4.24 DD177 pKa = 3.53 QPDD180 pKa = 3.94 VVHH183 pKa = 6.39 GAPRR187 pKa = 11.84 GRR189 pKa = 11.84 VALRR193 pKa = 11.84 PPNLL197 pKa = 3.4
Molecular weight: 21.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.313
IPC2_protein 9.443
IPC_protein 10.014
Toseland 10.365
ProMoST 10.058
Dawson 10.511
Bjellqvist 10.218
Wikipedia 10.701
Rodwell 10.73
Grimsley 10.57
Solomon 10.584
Lehninger 10.555
Nozaki 10.365
DTASelect 10.204
Thurlkill 10.394
EMBOSS 10.774
Sillero 10.438
Patrickios 10.438
IPC_peptide 10.584
IPC2_peptide 9.151
IPC2.peptide.svr19 8.578
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
77
0
77
35900
120
1629
466.2
51.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.12 ± 0.198
1.71 ± 0.131
5.457 ± 0.15
6.209 ± 0.28
3.836 ± 0.178
7.061 ± 0.154
2.32 ± 0.098
5.855 ± 0.199
4.591 ± 0.266
9.365 ± 0.197
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.922 ± 0.107
3.454 ± 0.131
6.17 ± 0.34
2.986 ± 0.137
6.345 ± 0.215
6.003 ± 0.209
7.437 ± 0.181
6.981 ± 0.127
1.276 ± 0.088
2.903 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here