Mycobacterium phage BigNuz

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pclasvirinae; Bignuzvirus; Mycobacterium virus Bignuz

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1JX39|G1JX39_9CAUD Uncharacterized protein OS=Mycobacterium phage BigNuz OX=1074309 GN=24 PE=4 SV=1
MM1 pKa = 7.53SNPTKK6 pKa = 10.95AEE8 pKa = 4.05DD9 pKa = 3.63YY10 pKa = 10.62EE11 pKa = 4.32FAFYY15 pKa = 10.3FSVKK19 pKa = 9.25GLKK22 pKa = 10.23VSGPGDD28 pKa = 4.09PDD30 pKa = 2.7TWYY33 pKa = 10.24PNIQYY38 pKa = 10.8GLRR41 pKa = 11.84GVGDD45 pKa = 4.32APPPLILQAYY55 pKa = 8.0LMNVGLHH62 pKa = 6.5SDD64 pKa = 3.73PEE66 pKa = 4.47SVRR69 pKa = 11.84FAILYY74 pKa = 6.72TEE76 pKa = 4.27PVTWTEE82 pKa = 3.96ASPKK86 pKa = 10.37DD87 pKa = 3.83VAALPGSVEE96 pKa = 4.51DD97 pKa = 3.66YY98 pKa = 11.24SFAIVGDD105 pKa = 3.83AVDD108 pKa = 3.91EE109 pKa = 4.93DD110 pKa = 4.33GSAFRR115 pKa = 11.84FGLVGSGEE123 pKa = 4.21YY124 pKa = 10.43GRR126 pKa = 11.84EE127 pKa = 3.66VSLFTLPIYY136 pKa = 10.79LDD138 pKa = 3.74SQDD141 pKa = 3.77QAGALHH147 pKa = 6.47NVALCYY153 pKa = 10.24APRR156 pKa = 11.84VSWSVWEE163 pKa = 4.0MDD165 pKa = 3.21AA166 pKa = 5.66

Molecular weight: 18.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1JX49|G1JX49_9CAUD Immunity repressor OS=Mycobacterium phage BigNuz OX=1074309 GN=34 PE=4 SV=1
MM1 pKa = 8.58VEE3 pKa = 4.01MIEE6 pKa = 4.84RR7 pKa = 11.84WRR9 pKa = 11.84IWQFAQSLSRR19 pKa = 11.84RR20 pKa = 11.84TVDD23 pKa = 2.99EE24 pKa = 4.2RR25 pKa = 11.84SATVRR30 pKa = 11.84RR31 pKa = 11.84MSEE34 pKa = 3.59WCGVAPEE41 pKa = 4.26YY42 pKa = 11.27ADD44 pKa = 3.74VEE46 pKa = 4.8HH47 pKa = 6.11VVSWLAEE54 pKa = 4.07GGNWSARR61 pKa = 11.84TRR63 pKa = 11.84WTYY66 pKa = 11.34YY67 pKa = 10.31GALSAWFLWLQQQGHH82 pKa = 5.58RR83 pKa = 11.84QDD85 pKa = 3.83NPMVMIGRR93 pKa = 11.84PKK95 pKa = 10.09RR96 pKa = 11.84PKK98 pKa = 9.75SVPRR102 pKa = 11.84PVSNHH107 pKa = 5.44DD108 pKa = 3.48VQRR111 pKa = 11.84LLAVRR116 pKa = 11.84AHH118 pKa = 6.16RR119 pKa = 11.84RR120 pKa = 11.84TRR122 pKa = 11.84AMILLAAFQGLRR134 pKa = 11.84VHH136 pKa = 7.38EE137 pKa = 4.11IAQIKK142 pKa = 10.02GEE144 pKa = 4.02HH145 pKa = 6.69LDD147 pKa = 3.64LVEE150 pKa = 4.1RR151 pKa = 11.84TMTVTGKK158 pKa = 11.13GNVTATLPLHH168 pKa = 5.86HH169 pKa = 6.81RR170 pKa = 11.84VVEE173 pKa = 4.17MAYY176 pKa = 9.4QMPRR180 pKa = 11.84KK181 pKa = 9.17GHH183 pKa = 6.4WFPGPDD189 pKa = 2.81RR190 pKa = 11.84GHH192 pKa = 5.67QRR194 pKa = 11.84RR195 pKa = 11.84EE196 pKa = 4.37SISGTIKK203 pKa = 10.18EE204 pKa = 4.1AMIRR208 pKa = 11.84AGVIGSAHH216 pKa = 6.14MLRR219 pKa = 11.84HH220 pKa = 5.19WFGTALLEE228 pKa = 4.59ANVDD232 pKa = 3.47LRR234 pKa = 11.84TVQEE238 pKa = 4.11LMRR241 pKa = 11.84HH242 pKa = 4.7QSLTSTEE249 pKa = 3.62IYY251 pKa = 10.3TRR253 pKa = 11.84VTDD256 pKa = 3.31QRR258 pKa = 11.84RR259 pKa = 11.84AEE261 pKa = 4.59GIEE264 pKa = 3.79RR265 pKa = 11.84LDD267 pKa = 3.8PFRR270 pKa = 11.84LAPARR275 pKa = 11.84RR276 pKa = 11.84VPDD279 pKa = 3.4RR280 pKa = 11.84LLAHH284 pKa = 7.06LVDD287 pKa = 4.99DD288 pKa = 5.04DD289 pKa = 4.64EE290 pKa = 6.17NGEE293 pKa = 4.19GTAMHH298 pKa = 7.32AAA300 pKa = 3.88

Molecular weight: 34.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

15783

32

1262

192.5

20.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.108 ± 0.466

0.969 ± 0.15

6.735 ± 0.198

6.019 ± 0.274

2.845 ± 0.178

9.06 ± 0.547

2.072 ± 0.172

4.34 ± 0.142

3.079 ± 0.172

8.211 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.894 ± 0.15

2.965 ± 0.167

6.209 ± 0.182

3.795 ± 0.32

7.128 ± 0.449

5.056 ± 0.232

6.254 ± 0.189

6.982 ± 0.292

1.92 ± 0.118

2.357 ± 0.123
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski