Amino acid dipepetide frequency for Mycobacterium phage BigNuz

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.728AlaAla: 16.728 ± 1.501
1.394AlaCys: 1.394 ± 0.339
8.174AlaAsp: 8.174 ± 0.774
10.455AlaGlu: 10.455 ± 1.086
3.168AlaPhe: 3.168 ± 0.552
9.441AlaGly: 9.441 ± 1.14
2.598AlaHis: 2.598 ± 0.414
6.273AlaIle: 6.273 ± 0.644
4.119AlaLys: 4.119 ± 0.687
10.518AlaLeu: 10.518 ± 0.843
2.535AlaMet: 2.535 ± 0.491
2.661AlaAsn: 2.661 ± 0.461
7.92AlaPro: 7.92 ± 0.84
3.738AlaGln: 3.738 ± 0.67
8.111AlaArg: 8.111 ± 0.894
5.703AlaSer: 5.703 ± 0.692
6.21AlaThr: 6.21 ± 0.658
6.653AlaVal: 6.653 ± 0.713
2.028AlaTrp: 2.028 ± 0.384
2.661AlaTyr: 2.661 ± 0.379
0.0AlaXaa: 0.0 ± 0.0
Cys
0.697CysAla: 0.697 ± 0.189
0.063CysCys: 0.063 ± 0.075
0.76CysAsp: 0.76 ± 0.226
0.824CysGlu: 0.824 ± 0.29
0.063CysPhe: 0.063 ± 0.07
1.331CysGly: 1.331 ± 0.295
0.38CysHis: 0.38 ± 0.144
0.253CysIle: 0.253 ± 0.17
0.253CysLys: 0.253 ± 0.124
0.634CysLeu: 0.634 ± 0.207
0.19CysMet: 0.19 ± 0.116
0.19CysAsn: 0.19 ± 0.116
0.95CysPro: 0.95 ± 0.31
0.38CysGln: 0.38 ± 0.172
1.077CysArg: 1.077 ± 0.279
0.317CysSer: 0.317 ± 0.154
0.57CysThr: 0.57 ± 0.166
0.253CysVal: 0.253 ± 0.117
0.127CysTrp: 0.127 ± 0.092
0.38CysTyr: 0.38 ± 0.154
0.0CysXaa: 0.0 ± 0.0
Asp
8.491AspAla: 8.491 ± 0.764
0.634AspCys: 0.634 ± 0.29
6.21AspAsp: 6.21 ± 0.796
6.717AspGlu: 6.717 ± 0.698
1.584AspPhe: 1.584 ± 0.26
6.273AspGly: 6.273 ± 0.727
1.331AspHis: 1.331 ± 0.279
1.774AspIle: 1.774 ± 0.295
1.521AspLys: 1.521 ± 0.291
5.386AspLeu: 5.386 ± 0.58
1.141AspMet: 1.141 ± 0.26
1.964AspAsn: 1.964 ± 0.387
4.372AspPro: 4.372 ± 0.601
1.901AspGln: 1.901 ± 0.322
4.689AspArg: 4.689 ± 0.487
2.535AspSer: 2.535 ± 0.351
2.978AspThr: 2.978 ± 0.459
4.435AspVal: 4.435 ± 0.449
1.647AspTrp: 1.647 ± 0.327
1.774AspTyr: 1.774 ± 0.397
0.0AspXaa: 0.0 ± 0.0
Glu
6.717GluAla: 6.717 ± 0.734
0.634GluCys: 0.634 ± 0.224
3.168GluAsp: 3.168 ± 0.429
2.218GluGlu: 2.218 ± 0.454
1.901GluPhe: 1.901 ± 0.335
4.562GluGly: 4.562 ± 0.493
2.028GluHis: 2.028 ± 0.387
2.915GluIle: 2.915 ± 0.445
1.394GluLys: 1.394 ± 0.296
7.667GluLeu: 7.667 ± 1.007
1.204GluMet: 1.204 ± 0.322
1.964GluAsn: 1.964 ± 0.316
3.612GluPro: 3.612 ± 0.475
2.661GluGln: 2.661 ± 0.391
4.182GluArg: 4.182 ± 0.486
3.105GluSer: 3.105 ± 0.442
3.041GluThr: 3.041 ± 0.445
4.119GluVal: 4.119 ± 0.643
1.394GluTrp: 1.394 ± 0.305
1.711GluTyr: 1.711 ± 0.262
0.0GluXaa: 0.0 ± 0.0
Phe
3.041PheAla: 3.041 ± 0.373
0.127PheCys: 0.127 ± 0.094
2.408PheAsp: 2.408 ± 0.466
1.774PheGlu: 1.774 ± 0.374
0.444PhePhe: 0.444 ± 0.144
4.245PheGly: 4.245 ± 0.651
0.38PheHis: 0.38 ± 0.151
1.457PheIle: 1.457 ± 0.316
1.331PheLys: 1.331 ± 0.223
1.711PheLeu: 1.711 ± 0.318
0.57PheMet: 0.57 ± 0.203
0.57PheAsn: 0.57 ± 0.171
1.901PhePro: 1.901 ± 0.375
0.887PheGln: 0.887 ± 0.275
1.584PheArg: 1.584 ± 0.284
1.267PheSer: 1.267 ± 0.326
1.838PheThr: 1.838 ± 0.346
1.584PheVal: 1.584 ± 0.262
0.317PheTrp: 0.317 ± 0.133
1.014PheTyr: 1.014 ± 0.203
0.0PheXaa: 0.0 ± 0.0
Gly
9.758GlyAla: 9.758 ± 1.016
0.444GlyCys: 0.444 ± 0.184
5.703GlyAsp: 5.703 ± 0.489
5.386GlyGlu: 5.386 ± 0.552
3.232GlyPhe: 3.232 ± 0.673
10.201GlyGly: 10.201 ± 2.088
1.394GlyHis: 1.394 ± 0.304
3.485GlyIle: 3.485 ± 0.487
2.978GlyLys: 2.978 ± 0.487
7.73GlyLeu: 7.73 ± 0.727
1.331GlyMet: 1.331 ± 0.262
2.851GlyAsn: 2.851 ± 0.526
4.372GlyPro: 4.372 ± 0.566
3.168GlyGln: 3.168 ± 0.653
5.576GlyArg: 5.576 ± 0.66
5.893GlySer: 5.893 ± 0.798
5.449GlyThr: 5.449 ± 0.76
7.097GlyVal: 7.097 ± 0.778
1.838GlyTrp: 1.838 ± 0.327
2.725GlyTyr: 2.725 ± 0.533
0.0GlyXaa: 0.0 ± 0.0
His
2.471HisAla: 2.471 ± 0.424
0.253HisCys: 0.253 ± 0.144
1.204HisAsp: 1.204 ± 0.318
1.331HisGlu: 1.331 ± 0.298
0.507HisPhe: 0.507 ± 0.184
1.014HisGly: 1.014 ± 0.264
0.824HisHis: 0.824 ± 0.26
0.76HisIle: 0.76 ± 0.248
0.57HisLys: 0.57 ± 0.191
1.457HisLeu: 1.457 ± 0.367
0.317HisMet: 0.317 ± 0.152
0.57HisAsn: 0.57 ± 0.207
1.331HisPro: 1.331 ± 0.335
1.204HisGln: 1.204 ± 0.25
2.154HisArg: 2.154 ± 0.395
0.57HisSer: 0.57 ± 0.175
1.521HisThr: 1.521 ± 0.297
1.521HisVal: 1.521 ± 0.362
0.507HisTrp: 0.507 ± 0.194
0.634HisTyr: 0.634 ± 0.247
0.0HisXaa: 0.0 ± 0.0
Ile
5.069IleAla: 5.069 ± 0.463
0.253IleCys: 0.253 ± 0.128
3.738IleAsp: 3.738 ± 0.493
4.245IleGlu: 4.245 ± 0.438
0.697IlePhe: 0.697 ± 0.25
3.865IleGly: 3.865 ± 0.505
0.57IleHis: 0.57 ± 0.221
0.95IleIle: 0.95 ± 0.179
1.584IleLys: 1.584 ± 0.306
2.154IleLeu: 2.154 ± 0.401
0.507IleMet: 0.507 ± 0.232
1.394IleAsn: 1.394 ± 0.3
2.028IlePro: 2.028 ± 0.363
1.964IleGln: 1.964 ± 0.469
3.802IleArg: 3.802 ± 0.523
2.028IleSer: 2.028 ± 0.395
3.358IleThr: 3.358 ± 0.403
3.485IleVal: 3.485 ± 0.483
0.824IleTrp: 0.824 ± 0.271
0.887IleTyr: 0.887 ± 0.297
0.0IleXaa: 0.0 ± 0.0
Lys
3.738LysAla: 3.738 ± 0.456
0.444LysCys: 0.444 ± 0.188
1.077LysAsp: 1.077 ± 0.227
0.887LysGlu: 0.887 ± 0.22
1.141LysPhe: 1.141 ± 0.281
2.471LysGly: 2.471 ± 0.364
0.634LysHis: 0.634 ± 0.226
0.95LysIle: 0.95 ± 0.217
0.697LysLys: 0.697 ± 0.188
3.358LysLeu: 3.358 ± 0.42
0.507LysMet: 0.507 ± 0.198
0.95LysAsn: 0.95 ± 0.287
2.725LysPro: 2.725 ± 0.42
1.077LysGln: 1.077 ± 0.221
2.598LysArg: 2.598 ± 0.465
2.028LysSer: 2.028 ± 0.344
2.281LysThr: 2.281 ± 0.4
2.281LysVal: 2.281 ± 0.32
0.19LysTrp: 0.19 ± 0.119
0.76LysTyr: 0.76 ± 0.186
0.0LysXaa: 0.0 ± 0.0
Leu
10.835LeuAla: 10.835 ± 0.937
0.697LeuCys: 0.697 ± 0.255
6.653LeuAsp: 6.653 ± 0.62
3.232LeuGlu: 3.232 ± 0.359
1.901LeuPhe: 1.901 ± 0.273
7.287LeuGly: 7.287 ± 0.721
1.457LeuHis: 1.457 ± 0.32
4.499LeuIle: 4.499 ± 0.453
2.725LeuLys: 2.725 ± 0.42
7.604LeuLeu: 7.604 ± 0.593
1.521LeuMet: 1.521 ± 0.338
3.041LeuAsn: 3.041 ± 0.504
5.956LeuPro: 5.956 ± 0.651
2.725LeuGln: 2.725 ± 0.526
5.639LeuArg: 5.639 ± 0.602
3.358LeuSer: 3.358 ± 0.468
4.752LeuThr: 4.752 ± 0.427
5.956LeuVal: 5.956 ± 0.56
0.95LeuTrp: 0.95 ± 0.247
1.331LeuTyr: 1.331 ± 0.341
0.0LeuXaa: 0.0 ± 0.0
Met
2.915MetAla: 2.915 ± 0.332
0.317MetCys: 0.317 ± 0.141
0.887MetAsp: 0.887 ± 0.223
0.634MetGlu: 0.634 ± 0.215
0.253MetPhe: 0.253 ± 0.121
1.077MetGly: 1.077 ± 0.285
0.253MetHis: 0.253 ± 0.119
0.824MetIle: 0.824 ± 0.307
0.317MetLys: 0.317 ± 0.15
1.204MetLeu: 1.204 ± 0.311
0.317MetMet: 0.317 ± 0.159
0.887MetAsn: 0.887 ± 0.282
1.394MetPro: 1.394 ± 0.289
0.887MetGln: 0.887 ± 0.264
1.521MetArg: 1.521 ± 0.362
2.408MetSer: 2.408 ± 0.436
1.838MetThr: 1.838 ± 0.358
0.824MetVal: 0.824 ± 0.237
0.127MetTrp: 0.127 ± 0.098
0.063MetTyr: 0.063 ± 0.068
0.0MetXaa: 0.0 ± 0.0
Asn
3.929AsnAla: 3.929 ± 0.54
0.38AsnCys: 0.38 ± 0.183
1.774AsnAsp: 1.774 ± 0.352
1.014AsnGlu: 1.014 ± 0.24
0.444AsnPhe: 0.444 ± 0.157
3.358AsnGly: 3.358 ± 0.536
0.634AsnHis: 0.634 ± 0.189
0.95AsnIle: 0.95 ± 0.227
0.507AsnLys: 0.507 ± 0.197
1.964AsnLeu: 1.964 ± 0.37
0.253AsnMet: 0.253 ± 0.126
0.824AsnAsn: 0.824 ± 0.255
2.851AsnPro: 2.851 ± 0.345
0.38AsnGln: 0.38 ± 0.118
2.725AsnArg: 2.725 ± 0.499
1.901AsnSer: 1.901 ± 0.515
1.901AsnThr: 1.901 ± 0.363
2.344AsnVal: 2.344 ± 0.364
1.014AsnTrp: 1.014 ± 0.218
0.507AsnTyr: 0.507 ± 0.171
0.0AsnXaa: 0.0 ± 0.0
Pro
7.35ProAla: 7.35 ± 0.749
0.253ProCys: 0.253 ± 0.134
4.182ProAsp: 4.182 ± 0.475
3.675ProGlu: 3.675 ± 0.564
1.647ProPhe: 1.647 ± 0.333
6.336ProGly: 6.336 ± 0.8
0.634ProHis: 0.634 ± 0.221
2.281ProIle: 2.281 ± 0.425
2.408ProLys: 2.408 ± 0.453
4.879ProLeu: 4.879 ± 0.447
1.014ProMet: 1.014 ± 0.227
2.344ProAsn: 2.344 ± 0.402
4.182ProPro: 4.182 ± 0.76
2.725ProGln: 2.725 ± 0.389
4.245ProArg: 4.245 ± 0.65
2.978ProSer: 2.978 ± 0.41
3.548ProThr: 3.548 ± 0.526
4.689ProVal: 4.689 ± 0.473
1.204ProTrp: 1.204 ± 0.264
1.521ProTyr: 1.521 ± 0.293
0.0ProXaa: 0.0 ± 0.0
Gln
4.435GlnAla: 4.435 ± 0.694
0.19GlnCys: 0.19 ± 0.127
1.584GlnAsp: 1.584 ± 0.38
1.521GlnGlu: 1.521 ± 0.298
2.091GlnPhe: 2.091 ± 0.541
3.168GlnGly: 3.168 ± 0.525
0.887GlnHis: 0.887 ± 0.244
1.964GlnIle: 1.964 ± 0.296
1.394GlnLys: 1.394 ± 0.337
2.851GlnLeu: 2.851 ± 0.508
1.141GlnMet: 1.141 ± 0.272
0.887GlnAsn: 0.887 ± 0.268
2.281GlnPro: 2.281 ± 0.44
2.281GlnGln: 2.281 ± 0.522
2.725GlnArg: 2.725 ± 0.367
1.964GlnSer: 1.964 ± 0.433
2.408GlnThr: 2.408 ± 0.435
2.725GlnVal: 2.725 ± 0.479
0.95GlnTrp: 0.95 ± 0.274
0.507GlnTyr: 0.507 ± 0.185
0.0GlnXaa: 0.0 ± 0.0
Arg
7.794ArgAla: 7.794 ± 0.735
0.887ArgCys: 0.887 ± 0.252
5.386ArgAsp: 5.386 ± 0.532
3.612ArgGlu: 3.612 ± 0.522
2.471ArgPhe: 2.471 ± 0.396
4.879ArgGly: 4.879 ± 0.692
1.647ArgHis: 1.647 ± 0.331
3.612ArgIle: 3.612 ± 0.5
3.358ArgLys: 3.358 ± 0.392
5.386ArgLeu: 5.386 ± 0.702
1.964ArgMet: 1.964 ± 0.326
1.964ArgAsn: 1.964 ± 0.372
3.485ArgPro: 3.485 ± 0.513
2.725ArgGln: 2.725 ± 0.455
7.16ArgArg: 7.16 ± 1.047
3.168ArgSer: 3.168 ± 0.605
4.055ArgThr: 4.055 ± 0.623
4.119ArgVal: 4.119 ± 0.591
1.647ArgTrp: 1.647 ± 0.338
1.964ArgTyr: 1.964 ± 0.377
0.0ArgXaa: 0.0 ± 0.0
Ser
6.336SerAla: 6.336 ± 0.765
0.317SerCys: 0.317 ± 0.145
3.295SerAsp: 3.295 ± 0.431
1.838SerGlu: 1.838 ± 0.308
1.014SerPhe: 1.014 ± 0.231
5.766SerGly: 5.766 ± 0.844
1.204SerHis: 1.204 ± 0.301
2.154SerIle: 2.154 ± 0.401
1.457SerLys: 1.457 ± 0.329
3.929SerLeu: 3.929 ± 0.426
1.014SerMet: 1.014 ± 0.227
1.647SerAsn: 1.647 ± 0.34
2.851SerPro: 2.851 ± 0.422
2.154SerGln: 2.154 ± 0.324
3.168SerArg: 3.168 ± 0.456
2.218SerSer: 2.218 ± 0.394
3.675SerThr: 3.675 ± 0.487
3.929SerVal: 3.929 ± 0.62
1.141SerTrp: 1.141 ± 0.245
1.457SerTyr: 1.457 ± 0.257
0.0SerXaa: 0.0 ± 0.0
Thr
7.54ThrAla: 7.54 ± 0.736
0.507ThrCys: 0.507 ± 0.187
3.675ThrAsp: 3.675 ± 0.391
4.309ThrGlu: 4.309 ± 0.545
1.838ThrPhe: 1.838 ± 0.434
6.4ThrGly: 6.4 ± 0.895
1.141ThrHis: 1.141 ± 0.259
3.422ThrIle: 3.422 ± 0.516
1.838ThrLys: 1.838 ± 0.278
4.309ThrLeu: 4.309 ± 0.635
1.014ThrMet: 1.014 ± 0.328
1.838ThrAsn: 1.838 ± 0.301
3.612ThrPro: 3.612 ± 0.475
2.408ThrGln: 2.408 ± 0.42
3.485ThrArg: 3.485 ± 0.443
2.661ThrSer: 2.661 ± 0.439
4.435ThrThr: 4.435 ± 0.455
5.703ThrVal: 5.703 ± 0.624
1.267ThrTrp: 1.267 ± 0.294
1.141ThrTyr: 1.141 ± 0.274
0.0ThrXaa: 0.0 ± 0.0
Val
8.364ValAla: 8.364 ± 0.629
1.204ValCys: 1.204 ± 0.289
4.879ValAsp: 4.879 ± 0.669
4.626ValGlu: 4.626 ± 0.564
2.344ValPhe: 2.344 ± 0.371
5.513ValGly: 5.513 ± 0.673
1.584ValHis: 1.584 ± 0.271
3.168ValIle: 3.168 ± 0.514
1.457ValLys: 1.457 ± 0.351
5.259ValLeu: 5.259 ± 0.505
1.457ValMet: 1.457 ± 0.42
1.964ValAsn: 1.964 ± 0.362
4.055ValPro: 4.055 ± 0.47
2.344ValGln: 2.344 ± 0.453
3.675ValArg: 3.675 ± 0.632
3.865ValSer: 3.865 ± 0.602
5.766ValThr: 5.766 ± 0.671
5.703ValVal: 5.703 ± 0.751
1.077ValTrp: 1.077 ± 0.239
1.521ValTyr: 1.521 ± 0.267
0.0ValXaa: 0.0 ± 0.0
Trp
1.584TrpAla: 1.584 ± 0.38
0.507TrpCys: 0.507 ± 0.17
0.95TrpAsp: 0.95 ± 0.23
0.76TrpGlu: 0.76 ± 0.206
1.141TrpPhe: 1.141 ± 0.306
1.394TrpGly: 1.394 ± 0.322
0.444TrpHis: 0.444 ± 0.161
1.014TrpIle: 1.014 ± 0.272
0.253TrpLys: 0.253 ± 0.111
1.964TrpLeu: 1.964 ± 0.358
0.507TrpMet: 0.507 ± 0.199
0.634TrpAsn: 0.634 ± 0.178
0.95TrpPro: 0.95 ± 0.299
1.394TrpGln: 1.394 ± 0.278
1.584TrpArg: 1.584 ± 0.407
1.331TrpSer: 1.331 ± 0.25
0.824TrpThr: 0.824 ± 0.21
1.014TrpVal: 1.014 ± 0.238
0.507TrpTrp: 0.507 ± 0.175
0.38TrpTyr: 0.38 ± 0.172
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.295TyrAla: 3.295 ± 0.535
0.19TyrCys: 0.19 ± 0.11
1.647TyrAsp: 1.647 ± 0.326
1.141TyrGlu: 1.141 ± 0.26
0.57TyrPhe: 0.57 ± 0.177
2.028TyrGly: 2.028 ± 0.361
0.697TyrHis: 0.697 ± 0.26
0.697TyrIle: 0.697 ± 0.197
0.634TyrLys: 0.634 ± 0.198
2.154TyrLeu: 2.154 ± 0.319
0.38TyrMet: 0.38 ± 0.162
0.507TyrAsn: 0.507 ± 0.16
1.267TyrPro: 1.267 ± 0.334
0.95TyrGln: 0.95 ± 0.262
1.584TyrArg: 1.584 ± 0.325
1.267TyrSer: 1.267 ± 0.335
2.091TyrThr: 2.091 ± 0.424
1.394TyrVal: 1.394 ± 0.324
0.444TyrTrp: 0.444 ± 0.17
0.634TyrTyr: 0.634 ± 0.196
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 82 proteins (15783 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski