Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Cupriavidus necator (strain JMP 134))

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Cupriavidus; Cupriavidus pinatubonensis

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6433 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q46NM3|Q46NM3_CUPPJ Ferredoxin:(2Fe-2S)-binding protein OS=Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) OX=264198 GN=Reut_C5888 PE=4 SV=1
MM1 pKa = 7.26ATLNGTNGIDD11 pKa = 3.95NITGTASSDD20 pKa = 3.69TILSGNGNDD29 pKa = 3.82YY30 pKa = 11.17VSAGAGNDD38 pKa = 3.58YY39 pKa = 11.28VDD41 pKa = 4.72GGNGDD46 pKa = 4.88DD47 pKa = 4.41IIEE50 pKa = 4.7GGSGDD55 pKa = 3.87DD56 pKa = 3.74TLLGGNGKK64 pKa = 9.6DD65 pKa = 3.63LVFGGLGNDD74 pKa = 3.61TLLGGNGVDD83 pKa = 3.45AVYY86 pKa = 10.4GGSGDD91 pKa = 4.6DD92 pKa = 4.1VIGSPGDD99 pKa = 3.57GTTSAGTDD107 pKa = 3.12NGGDD111 pKa = 3.49TLYY114 pKa = 11.46GDD116 pKa = 5.02GFDD119 pKa = 4.09TYY121 pKa = 11.98ADD123 pKa = 3.88LLLGRR128 pKa = 11.84GHH130 pKa = 7.21EE131 pKa = 4.33SLQPGNDD138 pKa = 3.25RR139 pKa = 11.84IYY141 pKa = 11.1GGNGDD146 pKa = 4.23DD147 pKa = 6.5LIYY150 pKa = 11.01GDD152 pKa = 4.66NGNNAAVGGNDD163 pKa = 3.61VIYY166 pKa = 10.52GGNGNDD172 pKa = 3.63TIYY175 pKa = 11.34GEE177 pKa = 4.54GGNDD181 pKa = 3.65VIQGGAGADD190 pKa = 3.66TLSGGDD196 pKa = 3.65GNDD199 pKa = 2.99VFAYY203 pKa = 10.32AAVSDD208 pKa = 4.07STAASMDD215 pKa = 3.78VITDD219 pKa = 3.8FVRR222 pKa = 11.84GADD225 pKa = 4.1HH226 pKa = 7.37IDD228 pKa = 3.3LRR230 pKa = 11.84PVLGDD235 pKa = 4.03SGFNWGGQTPTAHH248 pKa = 6.63GAWFQQSGGNTFVYY262 pKa = 10.66VDD264 pKa = 3.61VDD266 pKa = 3.83GNPAKK271 pKa = 10.92AEE273 pKa = 3.84MVIRR277 pKa = 11.84LNGLHH282 pKa = 7.44DD283 pKa = 3.81LTKK286 pKa = 10.63SDD288 pKa = 3.55FSGYY292 pKa = 10.49DD293 pKa = 2.86NHH295 pKa = 8.17APVAATDD302 pKa = 3.74SNAIRR307 pKa = 11.84EE308 pKa = 4.48DD309 pKa = 3.43NSPNPISGNVLSNDD323 pKa = 3.07SDD325 pKa = 3.74VDD327 pKa = 4.1GNVLAVANPGTYY339 pKa = 9.06IGQYY343 pKa = 8.48GTLSLHH349 pKa = 7.04ADD351 pKa = 3.43GSYY354 pKa = 10.93AYY356 pKa = 8.89TLNNGSGQVDD366 pKa = 3.8ALRR369 pKa = 11.84EE370 pKa = 4.25GEE372 pKa = 4.15QVQDD376 pKa = 3.52VFSYY380 pKa = 10.95SATDD384 pKa = 3.96GQASTPSTLSIRR396 pKa = 11.84ITGTNDD402 pKa = 2.67SATITASGNEE412 pKa = 3.93HH413 pKa = 5.67TAATEE418 pKa = 3.88TGGMNNATSAVALTGGTLTVHH439 pKa = 7.1DD440 pKa = 4.29VDD442 pKa = 3.82NGEE445 pKa = 3.96AHH447 pKa = 6.49FAAVPSASLAGTYY460 pKa = 8.33GTFTFDD466 pKa = 3.57SNTGAWTYY474 pKa = 10.58TLDD477 pKa = 4.04NSKK480 pKa = 11.19ADD482 pKa = 3.83ALTAGQQVTDD492 pKa = 3.49SLTVHH497 pKa = 6.5SADD500 pKa = 3.21QTAEE504 pKa = 3.56QTITVNITGSNDD516 pKa = 2.91NATITASSSEE526 pKa = 4.41DD527 pKa = 3.24IAVTEE532 pKa = 4.68AGGANNATPGDD543 pKa = 3.94PSASGTLTVHH553 pKa = 7.09DD554 pKa = 4.19VDD556 pKa = 4.15SGEE559 pKa = 3.98AHH561 pKa = 6.56FAAVPPQSLSGNYY574 pKa = 8.21GTFTFDD580 pKa = 2.86TSTGAWTYY588 pKa = 10.52TLDD591 pKa = 3.85NAKK594 pKa = 10.49ADD596 pKa = 3.88ALTAGQQVTDD606 pKa = 3.49SLTVHH611 pKa = 6.5SADD614 pKa = 3.21QTAEE618 pKa = 3.56QTITVNITGSNDD630 pKa = 2.78NATITTSPSEE640 pKa = 3.99DD641 pKa = 3.43MQVTEE646 pKa = 5.84AGGANNATPGDD657 pKa = 3.94PSASGTLTVHH667 pKa = 7.09DD668 pKa = 4.19VDD670 pKa = 4.15SGEE673 pKa = 3.98AHH675 pKa = 6.56FAAVPPQTLSGNYY688 pKa = 8.35GTFTFDD694 pKa = 3.55SNTGGWTYY702 pKa = 10.6TLDD705 pKa = 3.6NAKK708 pKa = 10.49ADD710 pKa = 3.88ALTAGQQVTDD720 pKa = 3.49SLTVHH725 pKa = 6.5SADD728 pKa = 3.21QTAEE732 pKa = 3.56QTITVNITGSNDD744 pKa = 2.78NATITTSPSEE754 pKa = 3.99DD755 pKa = 3.43MQVTEE760 pKa = 5.84AGGANNATPGDD771 pKa = 3.94PSASGTLTVHH781 pKa = 7.09DD782 pKa = 4.19VDD784 pKa = 4.15SGEE787 pKa = 3.98AHH789 pKa = 6.48FAAVPSQSLSGNYY802 pKa = 8.21GTFTFDD808 pKa = 3.87SNTEE812 pKa = 3.46AWTYY816 pKa = 10.27TLDD819 pKa = 3.86NGKK822 pKa = 10.14ADD824 pKa = 4.05ALTAGQHH831 pKa = 4.75VTDD834 pKa = 4.04SLTVHH839 pKa = 6.5SADD842 pKa = 3.21QTAEE846 pKa = 3.56QTITVNITGSNDD858 pKa = 2.91NATITASSSEE868 pKa = 4.05DD869 pKa = 3.44SLVTEE874 pKa = 5.01AGGANNVTPGDD885 pKa = 3.82PSASGTLTVHH895 pKa = 7.09DD896 pKa = 4.19VDD898 pKa = 4.8SGEE901 pKa = 3.85AHH903 pKa = 6.31YY904 pKa = 10.78AAVPPASLAGTYY916 pKa = 8.34GTFTFDD922 pKa = 3.57SNTGAWTYY930 pKa = 10.58TLDD933 pKa = 4.04NSKK936 pKa = 11.19ADD938 pKa = 3.83ALTAGQQVTDD948 pKa = 3.49SLTVHH953 pKa = 6.5SADD956 pKa = 3.21QTAEE960 pKa = 3.56QTITVNITGSNDD972 pKa = 2.91NATITASSSEE982 pKa = 4.41DD983 pKa = 3.24IAVTEE988 pKa = 4.68AGGANNATPGDD999 pKa = 3.94PSASGTLTVHH1009 pKa = 7.09DD1010 pKa = 4.19VDD1012 pKa = 4.15SGEE1015 pKa = 3.98AHH1017 pKa = 6.56FAAVPPQSLSGNYY1030 pKa = 8.21GTFTFDD1036 pKa = 2.86TSTGAWTYY1044 pKa = 10.52TLDD1047 pKa = 3.85NAKK1050 pKa = 10.49ADD1052 pKa = 3.88ALTAGQQVTDD1062 pKa = 3.49SLTVHH1067 pKa = 6.5SADD1070 pKa = 3.21QTAEE1074 pKa = 3.56QTITVNITGSNDD1086 pKa = 2.78NATITTSPSEE1096 pKa = 3.99DD1097 pKa = 3.43MQVTEE1102 pKa = 5.84AGGANNATPGDD1113 pKa = 3.94PSASGTLTVHH1123 pKa = 7.09DD1124 pKa = 4.19VDD1126 pKa = 4.15SGEE1129 pKa = 3.98AHH1131 pKa = 6.56FAAVPPQTLSGNYY1144 pKa = 8.35GTFTFDD1150 pKa = 3.55SNTGGWTYY1158 pKa = 10.6TLDD1161 pKa = 3.6NAKK1164 pKa = 10.49ADD1166 pKa = 3.88ALTAGQQVTDD1176 pKa = 3.49SLTVHH1181 pKa = 6.5SADD1184 pKa = 3.21QTAEE1188 pKa = 3.56QTITVNITGSNDD1200 pKa = 2.78NATITTSPSEE1210 pKa = 3.99DD1211 pKa = 3.43MQVTEE1216 pKa = 5.84AGGANNATPGDD1227 pKa = 3.94PSASGTLTVHH1237 pKa = 7.09DD1238 pKa = 4.19VDD1240 pKa = 4.15SGEE1243 pKa = 3.98AHH1245 pKa = 6.48FAAVPSQSLSGNYY1258 pKa = 8.21GTFTFDD1264 pKa = 3.87SNTEE1268 pKa = 3.46AWTYY1272 pKa = 10.27TLDD1275 pKa = 3.86NGKK1278 pKa = 10.14ADD1280 pKa = 4.05ALTAGQHH1287 pKa = 4.75VTDD1290 pKa = 4.04SLTVHH1295 pKa = 6.5SADD1298 pKa = 3.21QTAEE1302 pKa = 3.56QTITVNITGSNDD1314 pKa = 3.15TAVNAVPTSPQTVNEE1329 pKa = 4.02DD1330 pKa = 3.52TPLIFSTANGNGVSVSDD1347 pKa = 4.66ADD1349 pKa = 3.96NATHH1353 pKa = 6.65TVTLTSTHH1361 pKa = 6.2GAITLNGTAGLQFLTGDD1378 pKa = 4.09GAADD1382 pKa = 3.29STMTFTGSDD1391 pKa = 3.25AAINAALNGMSFLGDD1406 pKa = 3.12KK1407 pKa = 10.36DD1408 pKa = 4.03YY1409 pKa = 11.79AGTASMQIRR1418 pKa = 11.84TDD1420 pKa = 3.84DD1421 pKa = 3.77GTSADD1426 pKa = 3.92TDD1428 pKa = 3.99TIAIMVNPVNDD1439 pKa = 4.11PPVAADD1445 pKa = 3.61DD1446 pKa = 4.78VIYY1449 pKa = 10.82ASNNTGDD1456 pKa = 4.05IFSSPNGIVIPVSALLGNDD1475 pKa = 3.12TDD1477 pKa = 4.17IDD1479 pKa = 4.06GSALSITSVSAGSGAVSGVQFVPGSNDD1506 pKa = 3.1SLITFQTSNGSVGTFSYY1523 pKa = 10.02TVSDD1527 pKa = 4.13GAGGTSTATVTVNVVSTNGGSHH1549 pKa = 6.56VEE1551 pKa = 3.86LTGNYY1556 pKa = 7.7QASYY1560 pKa = 10.99LDD1562 pKa = 4.09GGSNGDD1568 pKa = 3.68SLTGAGAGDD1577 pKa = 3.95TFFGGAAADD1586 pKa = 3.96TLIGGAGNDD1595 pKa = 3.68VLRR1598 pKa = 11.84GGAGNDD1604 pKa = 3.59NLDD1607 pKa = 3.7GGAGIDD1613 pKa = 3.55MLDD1616 pKa = 3.7FSDD1619 pKa = 3.59ATGGINFTLVQSSAGTQVNLSSVGLDD1645 pKa = 2.74TDD1647 pKa = 4.08TYY1649 pKa = 11.21SNMEE1653 pKa = 3.91GVIGTKK1659 pKa = 10.61YY1660 pKa = 10.9SDD1662 pKa = 3.75TLTGSAGNDD1671 pKa = 2.96ILRR1674 pKa = 11.84GGGGNDD1680 pKa = 4.02IIDD1683 pKa = 4.0GGAGIDD1689 pKa = 4.47LLDD1692 pKa = 4.46FSDD1695 pKa = 3.68ATGAIAFTLTQSITNTVVNLTGVGLGSDD1723 pKa = 4.0TYY1725 pKa = 11.65RR1726 pKa = 11.84NMEE1729 pKa = 4.05GVIGSAFNDD1738 pKa = 3.74TLTGSTGNDD1747 pKa = 3.31VLRR1750 pKa = 11.84GGAGNDD1756 pKa = 3.43TLLGGDD1762 pKa = 4.45GNDD1765 pKa = 3.63MLIGGAGADD1774 pKa = 3.94TLTGGAGSDD1783 pKa = 3.1TFAFLRR1789 pKa = 11.84ADD1791 pKa = 3.44AASVDD1796 pKa = 4.12TITDD1800 pKa = 4.07FDD1802 pKa = 4.56AKK1804 pKa = 10.08PVASGGDD1811 pKa = 3.4ILDD1814 pKa = 4.45LADD1817 pKa = 4.89LLSGMSVTSANAAQFIHH1834 pKa = 6.87LSEE1837 pKa = 4.34VGGNTVVSVDD1847 pKa = 3.58RR1848 pKa = 11.84DD1849 pKa = 3.62GGGTGAAFQDD1859 pKa = 3.91VAVLQGVVGIDD1870 pKa = 4.46LNTLLINGNIHH1881 pKa = 5.19TVV1883 pKa = 2.92

Molecular weight: 188.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q46W84|Q46W84_CUPPJ Extradiol ring-cleavage dioxygenase class III enzyme subunit B OS=Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) OX=264198 GN=Reut_A3239 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LAII44 pKa = 4.0

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6433

0

6433

2109372

38

3081

327.9

35.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.907 ± 0.042

0.988 ± 0.01

5.284 ± 0.018

5.182 ± 0.03

3.474 ± 0.019

8.497 ± 0.033

2.275 ± 0.014

4.509 ± 0.022

2.975 ± 0.028

10.254 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.616 ± 0.014

2.658 ± 0.02

5.287 ± 0.021

3.779 ± 0.021

7.112 ± 0.035

5.414 ± 0.021

5.312 ± 0.021

7.731 ± 0.031

1.376 ± 0.013

2.369 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski