Streptomyces piniterrae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7124 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U0MW13|A0A4U0MW13_9ACTN Suppressor of fused domain protein OS=Streptomyces piniterrae OX=2571125 GN=FCH28_28285 PE=4 SV=1
MM1 pKa = 7.22GVVKK5 pKa = 9.14FVRR8 pKa = 11.84LEE10 pKa = 4.06CVKK13 pKa = 10.75QEE15 pKa = 4.03DD16 pKa = 4.07WNGEE20 pKa = 3.66DD21 pKa = 5.69DD22 pKa = 5.3IYY24 pKa = 10.81ITINGDD30 pKa = 3.31QVYY33 pKa = 8.05STSISEE39 pKa = 4.57DD40 pKa = 3.4DD41 pKa = 3.67NKK43 pKa = 10.94RR44 pKa = 11.84DD45 pKa = 3.17IDD47 pKa = 3.68QTFTYY52 pKa = 9.81QGDD55 pKa = 3.74VATVRR60 pKa = 11.84MYY62 pKa = 10.7EE63 pKa = 3.99YY64 pKa = 10.87DD65 pKa = 3.63STDD68 pKa = 3.7DD69 pKa = 4.84DD70 pKa = 4.89DD71 pKa = 6.09LLGEE75 pKa = 4.25QIVLGDD81 pKa = 3.7YY82 pKa = 11.61GEE84 pKa = 4.44MKK86 pKa = 9.67FTEE89 pKa = 4.45DD90 pKa = 3.15DD91 pKa = 3.37AYY93 pKa = 9.95YY94 pKa = 8.48TLSYY98 pKa = 10.54RR99 pKa = 11.84VIEE102 pKa = 4.26RR103 pKa = 3.59

Molecular weight: 12.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U0NB04|A0A4U0NB04_9ACTN ABC transporter permease OS=Streptomyces piniterrae OX=2571125 GN=FCH28_21070 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.48GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7124

0

7124

2346646

20

3772

329.4

35.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.669 ± 0.043

0.831 ± 0.007

5.964 ± 0.024

5.675 ± 0.027

2.709 ± 0.017

9.538 ± 0.031

2.38 ± 0.015

3.325 ± 0.019

2.288 ± 0.024

10.28 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.765 ± 0.012

1.78 ± 0.015

6.251 ± 0.026

2.861 ± 0.018

8.061 ± 0.033

5.054 ± 0.024

6.018 ± 0.022

8.01 ± 0.029

1.499 ± 0.014

2.041 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski