Amino acid dipepetide frequency for Streptomyces piniterrae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.055AlaAla: 22.055 ± 0.148
1.076AlaCys: 1.076 ± 0.02
8.47AlaAsp: 8.47 ± 0.067
8.892AlaGlu: 8.892 ± 0.092
3.447AlaPhe: 3.447 ± 0.04
13.287AlaGly: 13.287 ± 0.087
2.942AlaHis: 2.942 ± 0.039
3.682AlaIle: 3.682 ± 0.042
3.118AlaLys: 3.118 ± 0.05
14.505AlaLeu: 14.505 ± 0.111
2.554AlaMet: 2.554 ± 0.033
1.988AlaAsn: 1.988 ± 0.034
7.473AlaPro: 7.473 ± 0.077
3.898AlaGln: 3.898 ± 0.05
10.313AlaArg: 10.313 ± 0.089
5.709AlaSer: 5.709 ± 0.056
6.995AlaThr: 6.995 ± 0.057
11.774AlaVal: 11.774 ± 0.076
1.815AlaTrp: 1.815 ± 0.027
2.692AlaTyr: 2.692 ± 0.038
0.0AlaXaa: 0.0 ± 0.0
Cys
1.169CysAla: 1.169 ± 0.024
0.115CysCys: 0.115 ± 0.008
0.486CysAsp: 0.486 ± 0.014
0.407CysGlu: 0.407 ± 0.012
0.221CysPhe: 0.221 ± 0.009
1.035CysGly: 1.035 ± 0.025
0.2CysHis: 0.2 ± 0.008
0.213CysIle: 0.213 ± 0.01
0.123CysLys: 0.123 ± 0.008
0.783CysLeu: 0.783 ± 0.02
0.134CysMet: 0.134 ± 0.007
0.13CysAsn: 0.13 ± 0.008
0.518CysPro: 0.518 ± 0.017
0.178CysGln: 0.178 ± 0.009
0.626CysArg: 0.626 ± 0.018
0.441CysSer: 0.441 ± 0.013
0.551CysThr: 0.551 ± 0.016
0.669CysVal: 0.669 ± 0.016
0.137CysTrp: 0.137 ± 0.008
0.168CysTyr: 0.168 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.854AspAla: 7.854 ± 0.068
0.44AspCys: 0.44 ± 0.014
3.599AspAsp: 3.599 ± 0.047
3.893AspGlu: 3.893 ± 0.044
1.658AspPhe: 1.658 ± 0.03
6.552AspGly: 6.552 ± 0.058
1.502AspHis: 1.502 ± 0.024
2.022AspIle: 2.022 ± 0.033
1.241AspLys: 1.241 ± 0.031
6.035AspLeu: 6.035 ± 0.048
0.793AspMet: 0.793 ± 0.02
0.955AspAsn: 0.955 ± 0.02
4.465AspPro: 4.465 ± 0.046
1.561AspGln: 1.561 ± 0.028
4.91AspArg: 4.91 ± 0.054
2.535AspSer: 2.535 ± 0.036
3.13AspThr: 3.13 ± 0.041
4.372AspVal: 4.372 ± 0.048
1.024AspTrp: 1.024 ± 0.023
1.105AspTyr: 1.105 ± 0.022
0.0AspXaa: 0.0 ± 0.0
Glu
7.382GluAla: 7.382 ± 0.085
0.398GluCys: 0.398 ± 0.012
2.71GluAsp: 2.71 ± 0.036
3.499GluGlu: 3.499 ± 0.052
1.496GluPhe: 1.496 ± 0.026
4.274GluGly: 4.274 ± 0.045
1.503GluHis: 1.503 ± 0.022
2.308GluIle: 2.308 ± 0.033
1.458GluLys: 1.458 ± 0.03
6.945GluLeu: 6.945 ± 0.07
0.961GluMet: 0.961 ± 0.022
1.034GluAsn: 1.034 ± 0.024
3.407GluPro: 3.407 ± 0.043
2.214GluGln: 2.214 ± 0.033
5.51GluArg: 5.51 ± 0.051
2.637GluSer: 2.637 ± 0.035
2.914GluThr: 2.914 ± 0.037
4.313GluVal: 4.313 ± 0.053
0.761GluTrp: 0.761 ± 0.018
1.027GluTyr: 1.027 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
3.73PheAla: 3.73 ± 0.046
0.284PheCys: 0.284 ± 0.011
1.894PheAsp: 1.894 ± 0.029
1.382PheGlu: 1.382 ± 0.027
0.891PhePhe: 0.891 ± 0.023
2.981PheGly: 2.981 ± 0.038
0.659PheHis: 0.659 ± 0.018
0.795PheIle: 0.795 ± 0.021
0.517PheLys: 0.517 ± 0.015
2.588PheLeu: 2.588 ± 0.041
0.386PheMet: 0.386 ± 0.014
0.555PheAsn: 0.555 ± 0.015
1.439PhePro: 1.439 ± 0.023
0.667PheGln: 0.667 ± 0.017
1.861PheArg: 1.861 ± 0.032
1.433PheSer: 1.433 ± 0.026
1.938PheThr: 1.938 ± 0.033
2.109PheVal: 2.109 ± 0.031
0.398PheTrp: 0.398 ± 0.014
0.583PheTyr: 0.583 ± 0.016
0.0PheXaa: 0.0 ± 0.0
Gly
11.374GlyAla: 11.374 ± 0.094
0.907GlyCys: 0.907 ± 0.023
5.104GlyAsp: 5.104 ± 0.053
5.201GlyGlu: 5.201 ± 0.048
2.833GlyPhe: 2.833 ± 0.036
9.148GlyGly: 9.148 ± 0.09
2.425GlyHis: 2.425 ± 0.033
3.615GlyIle: 3.615 ± 0.048
2.634GlyLys: 2.634 ± 0.042
9.381GlyLeu: 9.381 ± 0.066
2.076GlyMet: 2.076 ± 0.027
1.798GlyAsn: 1.798 ± 0.031
5.472GlyPro: 5.472 ± 0.06
2.819GlyGln: 2.819 ± 0.037
7.91GlyArg: 7.91 ± 0.065
5.467GlySer: 5.467 ± 0.065
6.149GlyThr: 6.149 ± 0.053
7.143GlyVal: 7.143 ± 0.056
1.687GlyTrp: 1.687 ± 0.028
2.241GlyTyr: 2.241 ± 0.035
0.0GlyXaa: 0.0 ± 0.0
His
2.703HisAla: 2.703 ± 0.034
0.22HisCys: 0.22 ± 0.01
1.42HisAsp: 1.42 ± 0.027
1.199HisGlu: 1.199 ± 0.025
0.664HisPhe: 0.664 ± 0.017
2.565HisGly: 2.565 ± 0.039
0.759HisHis: 0.759 ± 0.023
0.795HisIle: 0.795 ± 0.016
0.421HisLys: 0.421 ± 0.013
2.501HisLeu: 2.501 ± 0.032
0.348HisMet: 0.348 ± 0.015
0.389HisAsn: 0.389 ± 0.015
1.923HisPro: 1.923 ± 0.032
0.738HisGln: 0.738 ± 0.018
2.176HisArg: 2.176 ± 0.033
1.101HisSer: 1.101 ± 0.021
1.43HisThr: 1.43 ± 0.028
1.566HisVal: 1.566 ± 0.024
0.392HisTrp: 0.392 ± 0.013
0.494HisTyr: 0.494 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
5.049IleAla: 5.049 ± 0.054
0.311IleCys: 0.311 ± 0.011
2.299IleAsp: 2.299 ± 0.03
2.104IleGlu: 2.104 ± 0.03
0.764IlePhe: 0.764 ± 0.019
3.791IleGly: 3.791 ± 0.042
0.646IleHis: 0.646 ± 0.016
0.935IleIle: 0.935 ± 0.021
0.802IleLys: 0.802 ± 0.021
2.432IleLeu: 2.432 ± 0.038
0.444IleMet: 0.444 ± 0.016
0.78IleAsn: 0.78 ± 0.022
1.935IlePro: 1.935 ± 0.031
0.776IleGln: 0.776 ± 0.019
2.391IleArg: 2.391 ± 0.032
1.766IleSer: 1.766 ± 0.027
2.305IleThr: 2.305 ± 0.031
2.788IleVal: 2.788 ± 0.039
0.398IleTrp: 0.398 ± 0.013
0.536IleTyr: 0.536 ± 0.017
0.0IleXaa: 0.0 ± 0.0
Lys
3.01LysAla: 3.01 ± 0.049
0.13LysCys: 0.13 ± 0.007
1.462LysAsp: 1.462 ± 0.036
1.232LysGlu: 1.232 ± 0.029
0.497LysPhe: 0.497 ± 0.016
1.959LysGly: 1.959 ± 0.038
0.46LysHis: 0.46 ± 0.016
0.952LysIle: 0.952 ± 0.024
0.994LysLys: 0.994 ± 0.036
2.135LysLeu: 2.135 ± 0.034
0.401LysMet: 0.401 ± 0.013
0.552LysAsn: 0.552 ± 0.017
1.503LysPro: 1.503 ± 0.028
0.732LysGln: 0.732 ± 0.02
1.575LysArg: 1.575 ± 0.028
1.258LysSer: 1.258 ± 0.026
1.339LysThr: 1.339 ± 0.03
1.928LysVal: 1.928 ± 0.038
0.294LysTrp: 0.294 ± 0.012
0.464LysTyr: 0.464 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
15.229LeuAla: 15.229 ± 0.103
0.878LeuCys: 0.878 ± 0.018
6.671LeuAsp: 6.671 ± 0.059
4.534LeuGlu: 4.534 ± 0.045
2.623LeuPhe: 2.623 ± 0.038
9.196LeuGly: 9.196 ± 0.07
2.397LeuHis: 2.397 ± 0.033
3.487LeuIle: 3.487 ± 0.041
2.052LeuLys: 2.052 ± 0.035
11.197LeuLeu: 11.197 ± 0.1
1.641LeuMet: 1.641 ± 0.031
1.685LeuAsn: 1.685 ± 0.027
6.649LeuPro: 6.649 ± 0.061
2.256LeuGln: 2.256 ± 0.03
8.704LeuArg: 8.704 ± 0.075
5.34LeuSer: 5.34 ± 0.053
6.788LeuThr: 6.788 ± 0.051
8.363LeuVal: 8.363 ± 0.074
1.298LeuTrp: 1.298 ± 0.027
1.816LeuTyr: 1.816 ± 0.031
0.0LeuXaa: 0.0 ± 0.0
Met
2.384MetAla: 2.384 ± 0.032
0.138MetCys: 0.138 ± 0.008
0.92MetAsp: 0.92 ± 0.022
0.786MetGlu: 0.786 ± 0.02
0.431MetPhe: 0.431 ± 0.015
1.412MetGly: 1.412 ± 0.024
0.364MetHis: 0.364 ± 0.011
0.633MetIle: 0.633 ± 0.018
0.43MetLys: 0.43 ± 0.012
1.703MetLeu: 1.703 ± 0.027
0.314MetMet: 0.314 ± 0.011
0.46MetAsn: 0.46 ± 0.014
1.17MetPro: 1.17 ± 0.024
0.445MetGln: 0.445 ± 0.015
1.417MetArg: 1.417 ± 0.025
1.298MetSer: 1.298 ± 0.023
1.52MetThr: 1.52 ± 0.022
1.284MetVal: 1.284 ± 0.026
0.218MetTrp: 0.218 ± 0.011
0.319MetTyr: 0.319 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.197AsnAla: 2.197 ± 0.037
0.182AsnCys: 0.182 ± 0.009
0.981AsnAsp: 0.981 ± 0.02
0.837AsnGlu: 0.837 ± 0.021
0.496AsnPhe: 0.496 ± 0.015
1.902AsnGly: 1.902 ± 0.029
0.412AsnHis: 0.412 ± 0.013
0.681AsnIle: 0.681 ± 0.018
0.463AsnLys: 0.463 ± 0.017
1.601AsnLeu: 1.601 ± 0.026
0.308AsnMet: 0.308 ± 0.013
0.441AsnAsn: 0.441 ± 0.015
1.377AsnPro: 1.377 ± 0.025
0.529AsnGln: 0.529 ± 0.016
1.289AsnArg: 1.289 ± 0.025
0.974AsnSer: 0.974 ± 0.021
1.079AsnThr: 1.079 ± 0.026
1.327AsnVal: 1.327 ± 0.027
0.305AsnTrp: 0.305 ± 0.013
0.421AsnTyr: 0.421 ± 0.015
0.0AsnXaa: 0.0 ± 0.0
Pro
8.821ProAla: 8.821 ± 0.077
0.378ProCys: 0.378 ± 0.013
4.712ProAsp: 4.712 ± 0.057
4.479ProGlu: 4.479 ± 0.042
1.558ProPhe: 1.558 ± 0.027
6.727ProGly: 6.727 ± 0.074
1.519ProHis: 1.519 ± 0.026
1.357ProIle: 1.357 ± 0.023
1.365ProLys: 1.365 ± 0.027
5.463ProLeu: 5.463 ± 0.058
1.028ProMet: 1.028 ± 0.02
0.927ProAsn: 0.927 ± 0.021
3.791ProPro: 3.791 ± 0.067
2.012ProGln: 2.012 ± 0.045
4.061ProArg: 4.061 ± 0.043
3.332ProSer: 3.332 ± 0.045
3.375ProThr: 3.375 ± 0.049
5.286ProVal: 5.286 ± 0.055
0.891ProTrp: 0.891 ± 0.021
1.433ProTyr: 1.433 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.8GlnAla: 3.8 ± 0.045
0.184GlnCys: 0.184 ± 0.009
1.463GlnAsp: 1.463 ± 0.024
1.515GlnGlu: 1.515 ± 0.028
0.685GlnPhe: 0.685 ± 0.018
2.374GlnGly: 2.374 ± 0.035
0.741GlnHis: 0.741 ± 0.019
1.134GlnIle: 1.134 ± 0.022
0.684GlnLys: 0.684 ± 0.021
3.257GlnLeu: 3.257 ± 0.038
0.513GlnMet: 0.513 ± 0.017
0.509GlnAsn: 0.509 ± 0.015
1.887GlnPro: 1.887 ± 0.039
1.426GlnGln: 1.426 ± 0.047
2.522GlnArg: 2.522 ± 0.037
1.301GlnSer: 1.301 ± 0.027
1.333GlnThr: 1.333 ± 0.026
2.247GlnVal: 2.247 ± 0.032
0.47GlnTrp: 0.47 ± 0.015
0.566GlnTyr: 0.566 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
9.828ArgAla: 9.828 ± 0.084
0.637ArgCys: 0.637 ± 0.022
4.25ArgAsp: 4.25 ± 0.047
4.674ArgGlu: 4.674 ± 0.047
2.284ArgPhe: 2.284 ± 0.035
5.827ArgGly: 5.827 ± 0.052
2.187ArgHis: 2.187 ± 0.035
3.403ArgIle: 3.403 ± 0.044
1.83ArgLys: 1.83 ± 0.032
8.754ArgLeu: 8.754 ± 0.083
1.784ArgMet: 1.784 ± 0.031
1.421ArgAsn: 1.421 ± 0.022
5.165ArgPro: 5.165 ± 0.06
2.412ArgGln: 2.412 ± 0.033
7.92ArgArg: 7.92 ± 0.084
4.076ArgSer: 4.076 ± 0.047
5.491ArgThr: 5.491 ± 0.054
5.488ArgVal: 5.488 ± 0.055
1.348ArgTrp: 1.348 ± 0.032
1.827ArgTyr: 1.827 ± 0.029
0.0ArgXaa: 0.0 ± 0.0
Ser
6.734SerAla: 6.734 ± 0.063
0.434SerCys: 0.434 ± 0.013
2.675SerAsp: 2.675 ± 0.031
2.462SerGlu: 2.462 ± 0.034
1.503SerPhe: 1.503 ± 0.026
6.047SerGly: 6.047 ± 0.065
1.095SerHis: 1.095 ± 0.024
1.473SerIle: 1.473 ± 0.024
1.119SerLys: 1.119 ± 0.027
4.798SerLeu: 4.798 ± 0.049
1.075SerMet: 1.075 ± 0.02
0.858SerAsn: 0.858 ± 0.023
3.245SerPro: 3.245 ± 0.047
1.292SerGln: 1.292 ± 0.031
3.705SerArg: 3.705 ± 0.041
2.862SerSer: 2.862 ± 0.051
2.94SerThr: 2.94 ± 0.039
4.094SerVal: 4.094 ± 0.045
0.913SerTrp: 0.913 ± 0.02
1.221SerTyr: 1.221 ± 0.024
0.0SerXaa: 0.0 ± 0.0
Thr
8.851ThrAla: 8.851 ± 0.073
0.437ThrCys: 0.437 ± 0.016
3.617ThrAsp: 3.617 ± 0.039
3.249ThrGlu: 3.249 ± 0.038
1.612ThrPhe: 1.612 ± 0.031
6.626ThrGly: 6.626 ± 0.061
1.241ThrHis: 1.241 ± 0.023
1.755ThrIle: 1.755 ± 0.028
1.213ThrLys: 1.213 ± 0.028
5.615ThrLeu: 5.615 ± 0.057
0.952ThrMet: 0.952 ± 0.021
0.99ThrAsn: 0.99 ± 0.023
4.164ThrPro: 4.164 ± 0.061
1.348ThrGln: 1.348 ± 0.028
3.785ThrArg: 3.785 ± 0.036
3.142ThrSer: 3.142 ± 0.04
3.747ThrThr: 3.747 ± 0.048
5.697ThrVal: 5.697 ± 0.053
0.878ThrTrp: 0.878 ± 0.023
1.258ThrTyr: 1.258 ± 0.026
0.0ThrXaa: 0.0 ± 0.0
Val
10.126ValAla: 10.126 ± 0.079
0.787ValCys: 0.787 ± 0.017
4.659ValAsp: 4.659 ± 0.051
4.417ValGlu: 4.417 ± 0.042
2.273ValPhe: 2.273 ± 0.036
6.355ValGly: 6.355 ± 0.065
1.963ValHis: 1.963 ± 0.033
2.969ValIle: 2.969 ± 0.039
1.657ValLys: 1.657 ± 0.034
9.157ValLeu: 9.157 ± 0.078
1.384ValMet: 1.384 ± 0.027
1.576ValAsn: 1.576 ± 0.03
5.072ValPro: 5.072 ± 0.048
2.062ValGln: 2.062 ± 0.031
6.827ValArg: 6.827 ± 0.061
4.02ValSer: 4.02 ± 0.04
5.098ValThr: 5.098 ± 0.042
7.145ValVal: 7.145 ± 0.067
1.081ValTrp: 1.081 ± 0.021
1.475ValTyr: 1.475 ± 0.026
0.0ValXaa: 0.0 ± 0.0
Trp
1.639TrpAla: 1.639 ± 0.032
0.175TrpCys: 0.175 ± 0.009
0.782TrpAsp: 0.782 ± 0.021
0.733TrpGlu: 0.733 ± 0.02
0.518TrpPhe: 0.518 ± 0.017
1.027TrpGly: 1.027 ± 0.025
0.398TrpHis: 0.398 ± 0.011
0.567TrpIle: 0.567 ± 0.015
0.364TrpLys: 0.364 ± 0.014
1.838TrpLeu: 1.838 ± 0.034
0.302TrpMet: 0.302 ± 0.011
0.382TrpAsn: 0.382 ± 0.015
0.788TrpPro: 0.788 ± 0.019
0.653TrpGln: 0.653 ± 0.016
1.305TrpArg: 1.305 ± 0.025
0.946TrpSer: 0.946 ± 0.018
0.952TrpThr: 0.952 ± 0.023
0.954TrpVal: 0.954 ± 0.02
0.339TrpTrp: 0.339 ± 0.013
0.329TrpTyr: 0.329 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.749TyrAla: 2.749 ± 0.039
0.193TyrCys: 0.193 ± 0.009
1.469TyrAsp: 1.469 ± 0.032
1.258TyrGlu: 1.258 ± 0.024
0.638TyrPhe: 0.638 ± 0.017
2.298TyrGly: 2.298 ± 0.03
0.389TyrHis: 0.389 ± 0.013
0.479TyrIle: 0.479 ± 0.015
0.392TyrLys: 0.392 ± 0.013
2.119TyrLeu: 2.119 ± 0.032
0.247TyrMet: 0.247 ± 0.011
0.371TyrAsn: 0.371 ± 0.014
1.074TyrPro: 1.074 ± 0.022
0.593TyrGln: 0.593 ± 0.016
1.795TyrArg: 1.795 ± 0.032
0.906TyrSer: 0.906 ± 0.02
1.103TyrThr: 1.103 ± 0.023
1.553TyrVal: 1.553 ± 0.027
0.348TyrTrp: 0.348 ± 0.013
0.435TyrTyr: 0.435 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7124 proteins (2346646 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski