Verticillium alfalfae (strain VaMs.102 / ATCC MYA-4576 / FGSC 10136) (Verticillium wilt of alfalfa) (Verticillium albo-atrum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Glomerellales; Plectosphaerellaceae; Verticillium; Verticillium alfalfae

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10233 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C9SNW9|C9SNW9_VERA1 Parasitic phase-specific protein PSP-1 OS=Verticillium alfalfae (strain VaMs.102 / ATCC MYA-4576 / FGSC 10136) OX=526221 GN=VDBG_06594 PE=4 SV=1
MM1 pKa = 6.68VASYY5 pKa = 9.58TKK7 pKa = 10.68LAIALTAASNVVAFTSIRR25 pKa = 11.84IAPTVEE31 pKa = 3.49AGKK34 pKa = 9.74EE35 pKa = 4.07VEE37 pKa = 4.1VSIVNDD43 pKa = 3.83LADD46 pKa = 4.17SDD48 pKa = 4.74SFDD51 pKa = 3.71AGFEE55 pKa = 3.98KK56 pKa = 10.6FRR58 pKa = 11.84VYY60 pKa = 10.82LATTPPGWGTGPACHH75 pKa = 6.82LVNATAIDD83 pKa = 3.81EE84 pKa = 4.5TSVKK88 pKa = 8.84VTIPPSVVPDD98 pKa = 4.01GSDD101 pKa = 3.2VMISVMEE108 pKa = 4.24FNEE111 pKa = 4.5DD112 pKa = 3.39PSLDD116 pKa = 3.86GPSGFQYY123 pKa = 11.17SNEE126 pKa = 3.78FTLNGGKK133 pKa = 10.31GEE135 pKa = 4.0WSKK138 pKa = 11.67PEE140 pKa = 4.0LEE142 pKa = 4.44GFNVGDD148 pKa = 4.32SDD150 pKa = 4.94TIPCEE155 pKa = 3.98AYY157 pKa = 10.23ACARR161 pKa = 11.84DD162 pKa = 4.14CMVQFYY168 pKa = 10.16PDD170 pKa = 3.44NKK172 pKa = 9.61EE173 pKa = 4.13DD174 pKa = 3.29EE175 pKa = 4.24SAYY178 pKa = 10.61RR179 pKa = 11.84KK180 pKa = 8.42TYY182 pKa = 10.26EE183 pKa = 4.5CTAEE187 pKa = 4.34CPGVDD192 pKa = 3.71YY193 pKa = 9.49PAWDD197 pKa = 3.94SLPGADD203 pKa = 4.38EE204 pKa = 4.48VPSEE208 pKa = 5.25DD209 pKa = 5.48DD210 pKa = 4.77GDD212 pKa = 5.17DD213 pKa = 3.92EE214 pKa = 7.46GDD216 pKa = 4.72DD217 pKa = 4.19DD218 pKa = 6.67DD219 pKa = 4.28EE220 pKa = 4.51TASISAAPSSIKK232 pKa = 9.98TSAATSGTATPSSGAATTILTTTGADD258 pKa = 3.21EE259 pKa = 4.75ASAPTEE265 pKa = 4.19TPSAAGRR272 pKa = 11.84HH273 pKa = 4.83TASLMLLAGIIGMCCLFF290 pKa = 4.34

Molecular weight: 30.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C9SYL7|C9SYL7_VERA1 Decarboxylase DEC1 OS=Verticillium alfalfae (strain VaMs.102 / ATCC MYA-4576 / FGSC 10136) OX=526221 GN=VDBG_09992 PE=4 SV=1
MM1 pKa = 7.56SFRR4 pKa = 11.84GGRR7 pKa = 11.84GAPRR11 pKa = 11.84GFGRR15 pKa = 11.84SWVEE19 pKa = 3.56AVEE22 pKa = 4.37VSSNATWVPRR32 pKa = 11.84RR33 pKa = 11.84KK34 pKa = 8.78WASSCTPARR43 pKa = 11.84VRR45 pKa = 11.84WSARR49 pKa = 11.84ASTPRR54 pKa = 11.84YY55 pKa = 8.38PSSTPRR61 pKa = 11.84CSSRR65 pKa = 11.84TRR67 pKa = 11.84PPLARR72 pKa = 11.84STKK75 pKa = 10.27SSAPSTKK82 pKa = 9.0STSPSSPPRR91 pKa = 11.84RR92 pKa = 11.84RR93 pKa = 11.84TWRR96 pKa = 11.84LRR98 pKa = 11.84RR99 pKa = 11.84SWRR102 pKa = 11.84RR103 pKa = 11.84RR104 pKa = 11.84RR105 pKa = 11.84FRR107 pKa = 11.84RR108 pKa = 11.84TRR110 pKa = 11.84RR111 pKa = 11.84CAEE114 pKa = 3.25GWTRR118 pKa = 11.84RR119 pKa = 11.84LQRR122 pKa = 11.84RR123 pKa = 11.84SWRR126 pKa = 11.84QRR128 pKa = 11.84LWWSRR133 pKa = 11.84RR134 pKa = 11.84RR135 pKa = 11.84RR136 pKa = 11.84RR137 pKa = 11.84LWRR140 pKa = 11.84SRR142 pKa = 11.84RR143 pKa = 11.84RR144 pKa = 11.84AEE146 pKa = 3.89GRR148 pKa = 11.84RR149 pKa = 11.84QGTRR153 pKa = 11.84RR154 pKa = 11.84LQQGRR159 pKa = 11.84RR160 pKa = 11.84ILRR163 pKa = 11.84LL164 pKa = 3.27

Molecular weight: 19.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10233

0

10233

4470746

29

4615

436.9

48.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.631 ± 0.022

1.263 ± 0.01

5.831 ± 0.016

5.908 ± 0.02

3.568 ± 0.015

7.2 ± 0.025

2.452 ± 0.011

4.45 ± 0.018

4.487 ± 0.021

8.688 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.186 ± 0.01

3.388 ± 0.014

6.286 ± 0.025

3.989 ± 0.019

6.513 ± 0.02

7.951 ± 0.028

5.968 ± 0.015

6.243 ± 0.017

1.474 ± 0.009

2.525 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski