Streptococcus phage CHPC1062
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G8FAC0|A0A3G8FAC0_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC1062 OX=2365021 GN=CHPC1062_0046 PE=4 SV=1
MM1 pKa = 7.3 SVSKK5 pKa = 9.08 EE6 pKa = 4.26 TIMQTLNLDD15 pKa = 3.8 EE16 pKa = 4.97 TDD18 pKa = 3.78 DD19 pKa = 3.8 TALIPAYY26 pKa = 9.63 IEE28 pKa = 4.23 SAQQYY33 pKa = 9.35 IINAVGSDD41 pKa = 3.23 PKK43 pKa = 10.89 FYY45 pKa = 10.75 DD46 pKa = 3.76 LEE48 pKa = 4.28 NVQPLFDD55 pKa = 3.7 TAVIALTSSYY65 pKa = 8.66 FTYY68 pKa = 10.07 RR69 pKa = 11.84 VALTDD74 pKa = 3.35 TATYY78 pKa = 9.11 PINLTLNSIIGQLRR92 pKa = 11.84 GLYY95 pKa = 8.41 ATYY98 pKa = 10.85 SEE100 pKa = 4.91 GVANGG105 pKa = 3.26
Molecular weight: 11.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.854
IPC2_protein 3.884
IPC_protein 3.783
Toseland 3.592
ProMoST 3.973
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.503
Solomon 3.757
Lehninger 3.719
Nozaki 3.923
DTASelect 4.113
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.91
Patrickios 0.223
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.828
Protein with the highest isoelectric point:
>tr|A0A3G8FAC7|A0A3G8FAC7_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC1062 OX=2365021 GN=CHPC1062_0049 PE=4 SV=1
MM1 pKa = 6.85 QXVDD5 pKa = 2.5 IGGRR9 pKa = 11.84 XRR11 pKa = 11.84 XXXKK15 pKa = 10.0 KK16 pKa = 10.07 KK17 pKa = 10.1 KK18 pKa = 9.41 RR19 pKa = 11.84 RR20 pKa = 11.84 KK21 pKa = 9.48 KK22 pKa = 10.2 KK23 pKa = 10.27 RR24 pKa = 11.84 EE25 pKa = 3.69 EE26 pKa = 3.84 KK27 pKa = 9.64 KK28 pKa = 10.41 KK29 pKa = 9.8 KK30 pKa = 9.52 RR31 pKa = 11.84 KK32 pKa = 7.14 RR33 pKa = 11.84 TRR35 pKa = 11.84 GRR37 pKa = 11.84 EE38 pKa = 3.8 KK39 pKa = 10.83
Molecular weight: 4.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.182
IPC2_protein 10.321
IPC_protein 11.754
Toseland 11.974
ProMoST 12.413
Dawson 11.989
Bjellqvist 11.93
Wikipedia 12.413
Rodwell 12.032
Grimsley 12.018
Solomon 12.427
Lehninger 12.34
Nozaki 11.974
DTASelect 11.93
Thurlkill 11.974
EMBOSS 12.457
Sillero 11.974
Patrickios 11.754
IPC_peptide 12.442
IPC2_peptide 11.403
IPC2.peptide.svr19 9.107
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
54
0
54
12345
39
1655
228.6
26.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.164 ± 0.496
0.599 ± 0.123
6.618 ± 0.253
6.974 ± 0.449
4.228 ± 0.185
6.505 ± 0.463
1.458 ± 0.103
6.796 ± 0.252
8.91 ± 0.474
7.882 ± 0.354
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.479 ± 0.187
6.326 ± 0.248
2.892 ± 0.218
4.237 ± 0.208
4.066 ± 0.23
6.213 ± 0.246
6.156 ± 0.295
5.67 ± 0.271
1.45 ± 0.139
4.334 ± 0.307
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here