Amino acid dipepetide frequency for Streptococcus phage CHPC1062

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.349AlaAla: 2.349 ± 0.899
0.243AlaCys: 0.243 ± 0.137
4.375AlaAsp: 4.375 ± 0.557
4.051AlaGlu: 4.051 ± 0.641
2.511AlaPhe: 2.511 ± 0.705
3.321AlaGly: 3.321 ± 0.702
0.567AlaHis: 0.567 ± 0.211
4.213AlaIle: 4.213 ± 0.664
6.076AlaLys: 6.076 ± 1.136
5.428AlaLeu: 5.428 ± 0.514
1.458AlaMet: 1.458 ± 0.38
4.294AlaAsn: 4.294 ± 0.78
1.215AlaPro: 1.215 ± 0.289
3.402AlaGln: 3.402 ± 0.543
2.592AlaArg: 2.592 ± 0.438
4.294AlaSer: 4.294 ± 0.603
3.808AlaThr: 3.808 ± 0.928
3.97AlaVal: 3.97 ± 0.629
0.972AlaTrp: 0.972 ± 0.233
2.511AlaTyr: 2.511 ± 0.5
0.0AlaXaa: 0.0 ± 0.0
Cys
0.081CysAla: 0.081 ± 0.067
0.0CysCys: 0.0 ± 0.0
0.567CysAsp: 0.567 ± 0.229
0.405CysGlu: 0.405 ± 0.232
0.405CysPhe: 0.405 ± 0.214
0.162CysGly: 0.162 ± 0.127
0.081CysHis: 0.081 ± 0.092
0.324CysIle: 0.324 ± 0.197
0.486CysLys: 0.486 ± 0.21
0.486CysLeu: 0.486 ± 0.228
0.081CysMet: 0.081 ± 0.087
0.405CysAsn: 0.405 ± 0.174
0.162CysPro: 0.162 ± 0.114
0.162CysGln: 0.162 ± 0.118
0.162CysArg: 0.162 ± 0.189
0.648CysSer: 0.648 ± 0.286
0.486CysThr: 0.486 ± 0.193
0.324CysVal: 0.324 ± 0.129
0.081CysTrp: 0.081 ± 0.083
0.486CysTyr: 0.486 ± 0.178
0.0CysXaa: 0.0 ± 0.0
Asp
3.564AspAla: 3.564 ± 0.511
0.324AspCys: 0.324 ± 0.215
4.699AspAsp: 4.699 ± 0.556
4.294AspGlu: 4.294 ± 0.711
3.889AspPhe: 3.889 ± 0.496
5.752AspGly: 5.752 ± 1.193
1.134AspHis: 1.134 ± 0.305
5.347AspIle: 5.347 ± 0.562
5.752AspLys: 5.752 ± 0.698
4.456AspLeu: 4.456 ± 0.714
1.863AspMet: 1.863 ± 0.406
4.699AspAsn: 4.699 ± 0.761
1.863AspPro: 1.863 ± 0.4
1.215AspGln: 1.215 ± 0.277
3.078AspArg: 3.078 ± 0.639
3.808AspSer: 3.808 ± 0.609
3.889AspThr: 3.889 ± 0.505
2.997AspVal: 2.997 ± 0.598
0.81AspTrp: 0.81 ± 0.251
2.754AspTyr: 2.754 ± 0.449
0.0AspXaa: 0.0 ± 0.0
Glu
4.051GluAla: 4.051 ± 0.455
0.324GluCys: 0.324 ± 0.134
4.375GluAsp: 4.375 ± 0.661
5.104GluGlu: 5.104 ± 0.89
2.511GluPhe: 2.511 ± 0.489
2.754GluGly: 2.754 ± 0.442
1.539GluHis: 1.539 ± 0.373
6.481GluIle: 6.481 ± 0.721
4.78GluLys: 4.78 ± 0.99
6.4GluLeu: 6.4 ± 0.82
2.511GluMet: 2.511 ± 0.442
4.213GluAsn: 4.213 ± 0.682
1.62GluPro: 1.62 ± 0.367
3.402GluGln: 3.402 ± 0.566
3.24GluArg: 3.24 ± 0.587
3.645GluSer: 3.645 ± 0.472
3.889GluThr: 3.889 ± 0.725
4.375GluVal: 4.375 ± 0.589
1.134GluTrp: 1.134 ± 0.309
3.402GluTyr: 3.402 ± 0.452
0.0GluXaa: 0.0 ± 0.0
Phe
2.349PheAla: 2.349 ± 0.439
0.243PheCys: 0.243 ± 0.228
3.483PheAsp: 3.483 ± 0.523
2.835PheGlu: 2.835 ± 0.583
1.539PhePhe: 1.539 ± 0.343
3.159PheGly: 3.159 ± 0.595
0.405PheHis: 0.405 ± 0.145
2.835PheIle: 2.835 ± 0.5
4.861PheLys: 4.861 ± 0.666
2.673PheLeu: 2.673 ± 0.449
0.648PheMet: 0.648 ± 0.193
3.321PheAsn: 3.321 ± 0.509
0.567PhePro: 0.567 ± 0.189
1.539PheGln: 1.539 ± 0.302
1.134PheArg: 1.134 ± 0.357
2.997PheSer: 2.997 ± 0.429
2.349PheThr: 2.349 ± 0.369
2.43PheVal: 2.43 ± 0.377
0.81PheTrp: 0.81 ± 0.242
2.106PheTyr: 2.106 ± 0.422
0.0PheXaa: 0.0 ± 0.0
Gly
2.592GlyAla: 2.592 ± 0.603
0.405GlyCys: 0.405 ± 0.219
3.97GlyAsp: 3.97 ± 0.626
3.889GlyGlu: 3.889 ± 0.557
2.835GlyPhe: 2.835 ± 0.412
4.132GlyGly: 4.132 ± 0.85
0.729GlyHis: 0.729 ± 0.247
5.266GlyIle: 5.266 ± 0.701
5.752GlyLys: 5.752 ± 0.917
6.076GlyLeu: 6.076 ± 0.752
1.62GlyMet: 1.62 ± 0.353
3.483GlyAsn: 3.483 ± 0.687
1.863GlyPro: 1.863 ± 0.644
3.24GlyGln: 3.24 ± 0.577
2.916GlyArg: 2.916 ± 0.504
3.564GlySer: 3.564 ± 0.656
4.132GlyThr: 4.132 ± 0.51
3.402GlyVal: 3.402 ± 0.576
1.296GlyTrp: 1.296 ± 0.392
3.889GlyTyr: 3.889 ± 0.567
0.0GlyXaa: 0.0 ± 0.0
His
0.486HisAla: 0.486 ± 0.161
0.081HisCys: 0.081 ± 0.085
0.891HisAsp: 0.891 ± 0.313
0.486HisGlu: 0.486 ± 0.241
0.729HisPhe: 0.729 ± 0.223
0.972HisGly: 0.972 ± 0.255
0.486HisHis: 0.486 ± 0.228
1.053HisIle: 1.053 ± 0.303
1.053HisLys: 1.053 ± 0.24
1.377HisLeu: 1.377 ± 0.222
0.243HisMet: 0.243 ± 0.156
1.053HisAsn: 1.053 ± 0.3
0.729HisPro: 0.729 ± 0.175
0.405HisGln: 0.405 ± 0.202
0.891HisArg: 0.891 ± 0.182
0.972HisSer: 0.972 ± 0.222
0.81HisThr: 0.81 ± 0.324
1.215HisVal: 1.215 ± 0.251
0.0HisTrp: 0.0 ± 0.0
0.648HisTyr: 0.648 ± 0.322
0.0HisXaa: 0.0 ± 0.0
Ile
4.699IleAla: 4.699 ± 0.92
0.486IleCys: 0.486 ± 0.207
5.347IleAsp: 5.347 ± 0.768
5.266IleGlu: 5.266 ± 0.81
1.701IlePhe: 1.701 ± 0.346
5.023IleGly: 5.023 ± 0.525
0.648IleHis: 0.648 ± 0.214
3.889IleIle: 3.889 ± 0.684
7.048IleLys: 7.048 ± 0.711
3.889IleLeu: 3.889 ± 0.564
2.025IleMet: 2.025 ± 0.44
4.294IleAsn: 4.294 ± 0.583
2.673IlePro: 2.673 ± 0.448
2.916IleGln: 2.916 ± 0.476
2.673IleArg: 2.673 ± 0.385
4.78IleSer: 4.78 ± 0.598
3.645IleThr: 3.645 ± 0.523
3.645IleVal: 3.645 ± 0.554
1.134IleTrp: 1.134 ± 0.279
2.187IleTyr: 2.187 ± 0.399
0.0IleXaa: 0.0 ± 0.0
Lys
5.833LysAla: 5.833 ± 0.679
0.324LysCys: 0.324 ± 0.214
5.185LysAsp: 5.185 ± 0.732
8.02LysGlu: 8.02 ± 0.882
3.727LysPhe: 3.727 ± 0.732
5.833LysGly: 5.833 ± 0.597
1.458LysHis: 1.458 ± 0.408
6.157LysIle: 6.157 ± 0.757
7.453LysLys: 7.453 ± 1.452
7.858LysLeu: 7.858 ± 0.839
1.944LysMet: 1.944 ± 0.348
5.266LysAsn: 5.266 ± 0.72
2.835LysPro: 2.835 ± 0.478
3.97LysGln: 3.97 ± 0.539
3.727LysArg: 3.727 ± 0.634
4.294LysSer: 4.294 ± 0.454
5.347LysThr: 5.347 ± 0.663
4.699LysVal: 4.699 ± 0.592
1.053LysTrp: 1.053 ± 0.217
4.132LysTyr: 4.132 ± 0.679
0.0LysXaa: 0.0 ± 0.0
Leu
6.157LeuAla: 6.157 ± 0.684
0.405LeuCys: 0.405 ± 0.203
4.942LeuAsp: 4.942 ± 0.671
6.724LeuGlu: 6.724 ± 0.754
2.754LeuPhe: 2.754 ± 0.399
5.59LeuGly: 5.59 ± 0.941
0.972LeuHis: 0.972 ± 0.263
3.645LeuIle: 3.645 ± 0.519
7.615LeuLys: 7.615 ± 0.62
5.914LeuLeu: 5.914 ± 0.817
3.078LeuMet: 3.078 ± 0.518
5.104LeuAsn: 5.104 ± 0.635
2.511LeuPro: 2.511 ± 0.431
1.863LeuGln: 1.863 ± 0.42
2.754LeuArg: 2.754 ± 0.634
5.671LeuSer: 5.671 ± 0.877
5.59LeuThr: 5.59 ± 0.799
4.132LeuVal: 4.132 ± 0.492
0.81LeuTrp: 0.81 ± 0.228
2.511LeuTyr: 2.511 ± 0.473
0.0LeuXaa: 0.0 ± 0.0
Met
1.62MetAla: 1.62 ± 0.336
0.081MetCys: 0.081 ± 0.079
1.215MetAsp: 1.215 ± 0.372
1.701MetGlu: 1.701 ± 0.479
1.215MetPhe: 1.215 ± 0.226
0.972MetGly: 0.972 ± 0.241
0.243MetHis: 0.243 ± 0.144
1.539MetIle: 1.539 ± 0.354
3.24MetLys: 3.24 ± 0.53
1.863MetLeu: 1.863 ± 0.328
0.486MetMet: 0.486 ± 0.19
1.134MetAsn: 1.134 ± 0.328
1.053MetPro: 1.053 ± 0.234
1.134MetGln: 1.134 ± 0.355
0.729MetArg: 0.729 ± 0.228
1.782MetSer: 1.782 ± 0.303
1.701MetThr: 1.701 ± 0.288
1.701MetVal: 1.701 ± 0.311
0.324MetTrp: 0.324 ± 0.14
1.053MetTyr: 1.053 ± 0.349
0.0MetXaa: 0.0 ± 0.0
Asn
5.023AsnAla: 5.023 ± 1.061
0.405AsnCys: 0.405 ± 0.16
3.159AsnAsp: 3.159 ± 0.553
3.564AsnGlu: 3.564 ± 0.678
2.997AsnPhe: 2.997 ± 0.441
6.076AsnGly: 6.076 ± 1.073
0.648AsnHis: 0.648 ± 0.202
3.97AsnIle: 3.97 ± 0.643
4.861AsnLys: 4.861 ± 0.572
5.266AsnLeu: 5.266 ± 0.522
1.053AsnMet: 1.053 ± 0.319
4.375AsnAsn: 4.375 ± 0.777
3.159AsnPro: 3.159 ± 0.649
2.592AsnGln: 2.592 ± 0.494
2.187AsnArg: 2.187 ± 0.489
3.97AsnSer: 3.97 ± 0.537
3.159AsnThr: 3.159 ± 0.573
4.051AsnVal: 4.051 ± 0.571
0.729AsnTrp: 0.729 ± 0.242
2.025AsnTyr: 2.025 ± 0.303
0.0AsnXaa: 0.0 ± 0.0
Pro
1.701ProAla: 1.701 ± 0.362
0.0ProCys: 0.0 ± 0.0
1.62ProAsp: 1.62 ± 0.419
1.782ProGlu: 1.782 ± 0.402
1.377ProPhe: 1.377 ± 0.308
1.215ProGly: 1.215 ± 0.496
0.243ProHis: 0.243 ± 0.128
2.187ProIle: 2.187 ± 0.309
4.051ProLys: 4.051 ± 0.483
2.106ProLeu: 2.106 ± 0.384
0.162ProMet: 0.162 ± 0.106
2.592ProAsn: 2.592 ± 0.42
0.324ProPro: 0.324 ± 0.219
1.539ProGln: 1.539 ± 0.31
0.81ProArg: 0.81 ± 0.268
2.187ProSer: 2.187 ± 0.338
2.187ProThr: 2.187 ± 0.493
1.053ProVal: 1.053 ± 0.345
0.567ProTrp: 0.567 ± 0.161
1.215ProTyr: 1.215 ± 0.283
0.0ProXaa: 0.0 ± 0.0
Gln
3.808GlnAla: 3.808 ± 0.588
0.162GlnCys: 0.162 ± 0.105
2.187GlnAsp: 2.187 ± 0.369
3.645GlnGlu: 3.645 ± 0.704
1.863GlnPhe: 1.863 ± 0.526
3.321GlnGly: 3.321 ± 0.729
0.405GlnHis: 0.405 ± 0.182
1.701GlnIle: 1.701 ± 0.332
3.483GlnLys: 3.483 ± 0.547
3.402GlnLeu: 3.402 ± 0.466
1.458GlnMet: 1.458 ± 0.356
2.592GlnAsn: 2.592 ± 0.398
0.486GlnPro: 0.486 ± 0.174
2.835GlnGln: 2.835 ± 0.584
1.701GlnArg: 1.701 ± 0.387
1.944GlnSer: 1.944 ± 0.366
2.997GlnThr: 2.997 ± 0.444
1.701GlnVal: 1.701 ± 0.41
0.567GlnTrp: 0.567 ± 0.222
2.025GlnTyr: 2.025 ± 0.32
0.081GlnXaa: 0.081 ± 0.082
Arg
2.511ArgAla: 2.511 ± 0.418
0.081ArgCys: 0.081 ± 0.094
2.268ArgAsp: 2.268 ± 0.429
2.511ArgGlu: 2.511 ± 0.604
1.944ArgPhe: 1.944 ± 0.365
2.349ArgGly: 2.349 ± 0.436
0.486ArgHis: 0.486 ± 0.201
2.511ArgIle: 2.511 ± 0.43
3.321ArgLys: 3.321 ± 0.576
3.808ArgLeu: 3.808 ± 0.64
1.053ArgMet: 1.053 ± 0.269
2.268ArgAsn: 2.268 ± 0.369
1.134ArgPro: 1.134 ± 0.306
2.187ArgGln: 2.187 ± 0.382
1.296ArgArg: 1.296 ± 0.327
1.782ArgSer: 1.782 ± 0.367
3.078ArgThr: 3.078 ± 0.669
2.673ArgVal: 2.673 ± 0.504
1.053ArgTrp: 1.053 ± 0.291
2.187ArgTyr: 2.187 ± 0.474
0.162ArgXaa: 0.162 ± 0.163
Ser
3.159SerAla: 3.159 ± 0.55
0.405SerCys: 0.405 ± 0.172
5.752SerAsp: 5.752 ± 0.516
4.051SerGlu: 4.051 ± 0.528
2.511SerPhe: 2.511 ± 0.437
3.321SerGly: 3.321 ± 0.534
0.891SerHis: 0.891 ± 0.24
5.752SerIle: 5.752 ± 0.585
4.861SerLys: 4.861 ± 0.775
3.97SerLeu: 3.97 ± 0.573
1.701SerMet: 1.701 ± 0.283
4.051SerAsn: 4.051 ± 0.463
1.944SerPro: 1.944 ± 0.357
2.592SerGln: 2.592 ± 0.488
3.483SerArg: 3.483 ± 0.666
3.321SerSer: 3.321 ± 0.629
3.889SerThr: 3.889 ± 0.552
3.564SerVal: 3.564 ± 0.568
0.486SerTrp: 0.486 ± 0.236
2.43SerTyr: 2.43 ± 0.508
0.0SerXaa: 0.0 ± 0.0
Thr
4.375ThrAla: 4.375 ± 0.639
0.486ThrCys: 0.486 ± 0.224
3.727ThrAsp: 3.727 ± 0.68
3.808ThrGlu: 3.808 ± 0.473
2.43ThrPhe: 2.43 ± 0.455
4.213ThrGly: 4.213 ± 0.559
1.134ThrHis: 1.134 ± 0.302
4.294ThrIle: 4.294 ± 1.015
4.861ThrLys: 4.861 ± 0.631
6.319ThrLeu: 6.319 ± 0.682
0.891ThrMet: 0.891 ± 0.241
4.213ThrAsn: 4.213 ± 0.566
1.539ThrPro: 1.539 ± 0.369
2.592ThrGln: 2.592 ± 0.513
2.106ThrArg: 2.106 ± 0.383
3.645ThrSer: 3.645 ± 0.389
2.916ThrThr: 2.916 ± 0.425
3.564ThrVal: 3.564 ± 0.535
1.134ThrTrp: 1.134 ± 0.321
3.321ThrTyr: 3.321 ± 0.609
0.0ThrXaa: 0.0 ± 0.0
Val
3.889ValAla: 3.889 ± 0.547
0.648ValCys: 0.648 ± 0.228
4.213ValAsp: 4.213 ± 0.514
3.808ValGlu: 3.808 ± 0.524
2.754ValPhe: 2.754 ± 0.477
3.645ValGly: 3.645 ± 0.556
0.648ValHis: 0.648 ± 0.215
3.24ValIle: 3.24 ± 0.371
4.861ValLys: 4.861 ± 0.503
2.835ValLeu: 2.835 ± 0.63
0.891ValMet: 0.891 ± 0.232
3.483ValAsn: 3.483 ± 0.552
1.863ValPro: 1.863 ± 0.414
1.782ValGln: 1.782 ± 0.484
2.025ValArg: 2.025 ± 0.448
4.861ValSer: 4.861 ± 0.749
4.213ValThr: 4.213 ± 0.722
3.645ValVal: 3.645 ± 0.593
0.972ValTrp: 0.972 ± 0.256
2.43ValTyr: 2.43 ± 0.513
0.0ValXaa: 0.0 ± 0.0
Trp
0.486TrpAla: 0.486 ± 0.174
0.0TrpCys: 0.0 ± 0.0
1.215TrpAsp: 1.215 ± 0.3
0.81TrpGlu: 0.81 ± 0.209
0.486TrpPhe: 0.486 ± 0.168
0.729TrpGly: 0.729 ± 0.315
0.324TrpHis: 0.324 ± 0.143
0.891TrpIle: 0.891 ± 0.231
1.296TrpLys: 1.296 ± 0.361
1.134TrpLeu: 1.134 ± 0.283
0.081TrpMet: 0.081 ± 0.088
0.486TrpAsn: 0.486 ± 0.217
0.081TrpPro: 0.081 ± 0.082
0.405TrpGln: 0.405 ± 0.176
1.053TrpArg: 1.053 ± 0.34
1.62TrpSer: 1.62 ± 0.472
1.377TrpThr: 1.377 ± 0.459
1.215TrpVal: 1.215 ± 0.273
0.405TrpTrp: 0.405 ± 0.172
0.405TrpTyr: 0.405 ± 0.165
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.916TyrAla: 2.916 ± 0.563
0.891TyrCys: 0.891 ± 0.345
2.997TyrAsp: 2.997 ± 0.616
2.997TyrGlu: 2.997 ± 0.57
2.106TyrPhe: 2.106 ± 0.378
2.511TyrGly: 2.511 ± 0.504
1.539TyrHis: 1.539 ± 0.421
2.673TyrIle: 2.673 ± 0.477
3.321TyrLys: 3.321 ± 0.415
3.564TyrLeu: 3.564 ± 0.548
1.458TyrMet: 1.458 ± 0.4
1.944TyrAsn: 1.944 ± 0.439
1.296TyrPro: 1.296 ± 0.34
2.592TyrGln: 2.592 ± 0.339
2.025TyrArg: 2.025 ± 0.313
2.349TyrSer: 2.349 ± 0.51
2.106TyrThr: 2.106 ± 0.404
2.268TyrVal: 2.268 ± 0.444
0.162TyrTrp: 0.162 ± 0.114
1.62TyrTyr: 1.62 ± 0.38
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.081XaaLys: 0.081 ± 0.082
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.081XaaArg: 0.081 ± 0.082
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.081XaaVal: 0.081 ± 0.082
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.162XaaXaa: 0.162 ± 0.163
Statistics based on 54 proteins (12345 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski