Metschnikowia sp. JCM 33374

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Metschnikowiaceae; Metschnikowia; unclassified Metschnikowia

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6652 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A512U876|A0A512U876_9ASCO Uncharacterized protein OS=Metschnikowia sp. JCM 33374 OX=2562755 GN=JCM33374_g943 PE=4 SV=1
MM1 pKa = 7.54GSAPSDD7 pKa = 3.74SIFNDD12 pKa = 3.38VLDD15 pKa = 4.59GNPPGEE21 pKa = 4.41SIKK24 pKa = 10.85PPFPTATSMGEE35 pKa = 4.0RR36 pKa = 11.84IVGLNNRR43 pKa = 11.84EE44 pKa = 4.06EE45 pKa = 4.18KK46 pKa = 10.55CIQGEE51 pKa = 4.28TRR53 pKa = 11.84ATNEE57 pKa = 3.64VGKK60 pKa = 10.42ADD62 pKa = 4.26HH63 pKa = 6.76LVEE66 pKa = 4.58HH67 pKa = 6.79CPIDD71 pKa = 4.0EE72 pKa = 4.81DD73 pKa = 5.29SSDD76 pKa = 3.77EE77 pKa = 4.07EE78 pKa = 4.56NINLIPRR85 pKa = 11.84CKK87 pKa = 10.33FIITSQDD94 pKa = 3.44DD95 pKa = 3.78NCTPEE100 pKa = 4.35AVGNGTPNDD109 pKa = 3.78YY110 pKa = 9.82TSSSEE115 pKa = 3.96TPEE118 pKa = 4.95IIGQVYY124 pKa = 9.78LVQSDD129 pKa = 3.72NDD131 pKa = 4.03CYY133 pKa = 10.93RR134 pKa = 11.84SDD136 pKa = 3.51VLFEE140 pKa = 4.68GFSSYY145 pKa = 7.24QTVNRR150 pKa = 11.84NQPRR154 pKa = 11.84RR155 pKa = 11.84TTKK158 pKa = 10.53NSINAFRR165 pKa = 11.84RR166 pKa = 11.84SQNGHH171 pKa = 5.36FSYY174 pKa = 11.07YY175 pKa = 10.05EE176 pKa = 4.09SPHH179 pKa = 6.38EE180 pKa = 4.26EE181 pKa = 4.27SQSSDD186 pKa = 2.96SVYY189 pKa = 11.26DD190 pKa = 3.49EE191 pKa = 6.01DD192 pKa = 5.21EE193 pKa = 4.6FSTEE197 pKa = 3.74QAIDD201 pKa = 3.26EE202 pKa = 4.49GSPLKK207 pKa = 10.6KK208 pKa = 9.77DD209 pKa = 3.25TSYY212 pKa = 11.58SCDD215 pKa = 3.98PIYY218 pKa = 10.76LLSDD222 pKa = 3.35EE223 pKa = 4.73TTLSDD228 pKa = 3.93DD229 pKa = 3.64YY230 pKa = 11.86SYY232 pKa = 11.93GDD234 pKa = 3.51SNTYY238 pKa = 10.6GNSSRR243 pKa = 11.84PYY245 pKa = 10.05IPDD248 pKa = 3.44STSDD252 pKa = 3.64SQDD255 pKa = 3.12STDD258 pKa = 3.97SQDD261 pKa = 3.4STDD264 pKa = 4.01SQDD267 pKa = 3.4STDD270 pKa = 4.01SQDD273 pKa = 3.4STDD276 pKa = 4.01SQDD279 pKa = 3.45STDD282 pKa = 3.66SQVPTIEE289 pKa = 4.71DD290 pKa = 3.54TTSDD294 pKa = 4.28DD295 pKa = 3.93NEE297 pKa = 4.63SMDD300 pKa = 3.54TYY302 pKa = 11.55VYY304 pKa = 10.37ISSKK308 pKa = 8.58EE309 pKa = 3.88HH310 pKa = 5.7SSSYY314 pKa = 9.93VAEE317 pKa = 4.66DD318 pKa = 5.27DD319 pKa = 4.27IVLDD323 pKa = 4.13PEE325 pKa = 4.47DD326 pKa = 4.76EE327 pKa = 4.32EE328 pKa = 4.84VLSQEE333 pKa = 4.85KK334 pKa = 10.53YY335 pKa = 6.79PTPPEE340 pKa = 4.38PPNPPDD346 pKa = 3.82PSNPPEE352 pKa = 4.32PPKK355 pKa = 10.6PPVPNRR361 pKa = 11.84YY362 pKa = 8.81KK363 pKa = 10.79ASPNSFPQSQEE374 pKa = 3.89TPP376 pKa = 3.3

Molecular weight: 41.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A512UGH6|A0A512UGH6_9ASCO Uncharacterized protein OS=Metschnikowia sp. JCM 33374 OX=2562755 GN=JCM33374_g3829 PE=4 SV=1
MM1 pKa = 7.83ANRR4 pKa = 11.84TVGSCVGSSGKK15 pKa = 9.67SSRR18 pKa = 11.84SLTICVRR25 pKa = 11.84AHH27 pKa = 6.38NGHH30 pKa = 5.91SLRR33 pKa = 11.84GGVRR37 pKa = 11.84TGQSRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84EE45 pKa = 4.27WNNHH49 pKa = 3.48NRR51 pKa = 11.84SLGSISAAYY60 pKa = 9.61SLSGGVRR67 pKa = 11.84SCPSRR72 pKa = 11.84GGEE75 pKa = 4.01WNNHH79 pKa = 3.81NRR81 pKa = 11.84SLGSISAGQQEE92 pKa = 5.37CKK94 pKa = 10.2NQSKK98 pKa = 10.27VEE100 pKa = 3.99VEE102 pKa = 4.12EE103 pKa = 3.99TRR105 pKa = 11.84NNHH108 pKa = 5.47TGVLAPSGGYY118 pKa = 7.05SQRR121 pKa = 11.84VVVRR125 pKa = 11.84TRR127 pKa = 11.84EE128 pKa = 4.01VEE130 pKa = 4.16EE131 pKa = 4.0VRR133 pKa = 11.84NNHH136 pKa = 4.66NWRR139 pKa = 11.84LGMEE143 pKa = 4.35QSTKK147 pKa = 11.1DD148 pKa = 3.48PVQVEE153 pKa = 4.54VEE155 pKa = 4.13NGTTTTGVLAPSVSLSGGKK174 pKa = 9.41ISSKK178 pKa = 11.02

Molecular weight: 19.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6652

0

6652

3060062

66

5577

460.0

51.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.687 ± 0.033

1.186 ± 0.012

5.712 ± 0.026

6.418 ± 0.035

4.389 ± 0.023

5.646 ± 0.034

2.364 ± 0.014

5.494 ± 0.025

6.417 ± 0.036

8.869 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.015 ± 0.013

4.789 ± 0.022

5.001 ± 0.031

3.881 ± 0.025

4.481 ± 0.024

9.716 ± 0.055

6.324 ± 0.094

6.386 ± 0.036

1.078 ± 0.012

3.15 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski