Measles virus (strain Ichinose-B95a) (MeV) (Subacute sclerose panencephalitis virus)
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q786F2|HEMA_MEASC Hemagglutinin glycoprotein OS=Measles virus (strain Ichinose-B95a) OX=645098 GN=H PE=1 SV=1
MM1 pKa = 8.15 AEE3 pKa = 3.63 EE4 pKa = 3.91 QARR7 pKa = 11.84 HH8 pKa = 4.84 VKK10 pKa = 10.61 NGLEE14 pKa = 4.48 CIRR17 pKa = 11.84 ALKK20 pKa = 10.54 AEE22 pKa = 5.1 PIGSLAVEE30 pKa = 4.26 EE31 pKa = 5.78 AMAAWSEE38 pKa = 4.06 ISDD41 pKa = 3.99 NPGQDD46 pKa = 2.54 RR47 pKa = 11.84 ATCKK51 pKa = 10.55 EE52 pKa = 4.09 EE53 pKa = 3.89 EE54 pKa = 4.39 AGSSGLSKK62 pKa = 10.51 PCLSAIGSTEE72 pKa = 3.52 GGAPRR77 pKa = 11.84 IRR79 pKa = 11.84 GQGSGEE85 pKa = 4.09 SDD87 pKa = 3.97 DD88 pKa = 4.82 DD89 pKa = 4.92 AEE91 pKa = 4.47 TLGIPSRR98 pKa = 11.84 NLQASSTGLQCYY110 pKa = 8.88 HH111 pKa = 7.22 VYY113 pKa = 10.56 DD114 pKa = 4.08 HH115 pKa = 7.03 SGEE118 pKa = 4.13 AVKK121 pKa = 10.71 GIQDD125 pKa = 3.6 ADD127 pKa = 3.98 SIMVQSGLDD136 pKa = 3.52 GDD138 pKa = 4.4 STLSGGDD145 pKa = 3.82 DD146 pKa = 3.42 EE147 pKa = 6.08 SEE149 pKa = 4.18 NSDD152 pKa = 3.3 VDD154 pKa = 3.07 IGEE157 pKa = 4.99 PDD159 pKa = 3.41 TEE161 pKa = 4.81 GYY163 pKa = 10.86 AITDD167 pKa = 3.66 RR168 pKa = 11.84 GSAPISMGFRR178 pKa = 11.84 ASDD181 pKa = 3.44 VEE183 pKa = 4.22 TAEE186 pKa = 4.19 GGEE189 pKa = 3.8 IHH191 pKa = 7.05 EE192 pKa = 4.42 LLKK195 pKa = 10.95 LQSRR199 pKa = 11.84 GNNFPKK205 pKa = 10.39 LGKK208 pKa = 7.92 TLNVPPPPNPSRR220 pKa = 11.84 ASTSEE225 pKa = 3.87 TPIKK229 pKa = 9.98 KK230 pKa = 8.95 GHH232 pKa = 5.76 RR233 pKa = 11.84 RR234 pKa = 11.84 EE235 pKa = 5.2 IGLIWNGDD243 pKa = 3.23 RR244 pKa = 11.84 VFIDD248 pKa = 3.78 RR249 pKa = 11.84 WCNPMCSKK257 pKa = 9.47 VTLGTIRR264 pKa = 11.84 ARR266 pKa = 11.84 CTCGEE271 pKa = 4.22 CPRR274 pKa = 11.84 VCEE277 pKa = 4.19 QCRR280 pKa = 11.84 TDD282 pKa = 3.22 TGVDD286 pKa = 3.22 TRR288 pKa = 11.84 IWYY291 pKa = 9.3 HH292 pKa = 5.99 NLPEE296 pKa = 5.03 IPEE299 pKa = 4.01
Molecular weight: 32.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.695
IPC2_protein 4.698
IPC_protein 4.635
Toseland 4.495
ProMoST 4.711
Dawson 4.584
Bjellqvist 4.749
Wikipedia 4.444
Rodwell 4.482
Grimsley 4.406
Solomon 4.584
Lehninger 4.533
Nozaki 4.685
DTASelect 4.838
Thurlkill 4.495
EMBOSS 4.469
Sillero 4.762
Patrickios 3.872
IPC_peptide 4.584
IPC2_peptide 4.749
IPC2.peptide.svr19 4.691
Protein with the highest isoelectric point:
>sp|Q9YZN9|C_MEASC Protein C OS=Measles virus (strain Ichinose-B95a) OX=645098 GN=P/V/C PE=3 SV=1
MM1 pKa = 7.9 SKK3 pKa = 9.54 TDD5 pKa = 3.3 WNASGLSRR13 pKa = 11.84 PSPSAHH19 pKa = 5.55 WPSRR23 pKa = 11.84 KK24 pKa = 8.87 PWQHH28 pKa = 3.85 GQKK31 pKa = 10.26 YY32 pKa = 7.23 QTTQDD37 pKa = 3.16 RR38 pKa = 11.84 TEE40 pKa = 3.99 PPARR44 pKa = 11.84 KK45 pKa = 8.91 RR46 pKa = 11.84 RR47 pKa = 11.84 QAVRR51 pKa = 11.84 VSANHH56 pKa = 7.14 ASQQLDD62 pKa = 3.29 QLKK65 pKa = 10.12 AVHH68 pKa = 6.38 LASAVRR74 pKa = 11.84 DD75 pKa = 3.67 LEE77 pKa = 4.53 KK78 pKa = 11.26 AMTTLKK84 pKa = 10.47 LWEE87 pKa = 4.53 SPQEE91 pKa = 4.11 ISRR94 pKa = 11.84 HH95 pKa = 4.01 QALGYY100 pKa = 9.1 SVIMFMITAVKK111 pKa = 10.1 RR112 pKa = 11.84 LRR114 pKa = 11.84 EE115 pKa = 4.28 SKK117 pKa = 10.12 MLTLSWFNQALMVIAPSQEE136 pKa = 3.32 EE137 pKa = 4.5 TMNLKK142 pKa = 8.49 TAMWILANLIPRR154 pKa = 11.84 DD155 pKa = 4.0 MLSLTGDD162 pKa = 4.77 LLPSLWGSGLLMLKK176 pKa = 9.76 LQKK179 pKa = 10.13 EE180 pKa = 4.71 GRR182 pKa = 11.84 STSSS186 pKa = 2.66
Molecular weight: 21.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.201
IPC2_protein 9.516
IPC_protein 9.736
Toseland 10.687
ProMoST 10.189
Dawson 10.745
Bjellqvist 10.35
Wikipedia 10.877
Rodwell 11.184
Grimsley 10.774
Solomon 10.818
Lehninger 10.804
Nozaki 10.643
DTASelect 10.35
Thurlkill 10.657
EMBOSS 11.067
Sillero 10.672
Patrickios 10.921
IPC_peptide 10.833
IPC2_peptide 8.756
IPC2.peptide.svr19 8.758
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
5202
186
2183
650.3
72.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.209 ± 0.579
1.788 ± 0.307
5.536 ± 0.429
5.709 ± 0.482
3.114 ± 0.529
7.017 ± 0.766
2.345 ± 0.46
7.132 ± 0.422
4.998 ± 0.347
10.554 ± 0.468
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.499 ± 0.178
4.191 ± 0.289
4.748 ± 0.349
3.479 ± 0.378
6.075 ± 0.335
8.612 ± 0.505
5.498 ± 0.12
6.19 ± 0.521
1.096 ± 0.186
3.21 ± 0.493
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here