Streptomyces sp. WM4235

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6275 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M9YF91|A0A0M9YF91_9ACTN Iron-sulfur protein OS=Streptomyces sp. WM4235 OX=1415551 GN=ADK55_22405 PE=4 SV=1
MM1 pKa = 6.51ITMYY5 pKa = 10.56AWPSTADD12 pKa = 3.82GPDD15 pKa = 4.02ALPMVHH21 pKa = 6.57FTTDD25 pKa = 3.39DD26 pKa = 3.35QSSGEE31 pKa = 4.08FTPEE35 pKa = 3.68GTPVWMFDD43 pKa = 2.96GAIRR47 pKa = 11.84DD48 pKa = 4.01GGWAEE53 pKa = 4.0FSDD56 pKa = 4.24FEE58 pKa = 4.42GWSTPAEE65 pKa = 4.23GWQAFYY71 pKa = 10.88RR72 pKa = 11.84RR73 pKa = 11.84EE74 pKa = 4.01DD75 pKa = 3.66DD76 pKa = 4.09LLAVTGPGACEE87 pKa = 3.34GWYY90 pKa = 9.54QGKK93 pKa = 10.13LGADD97 pKa = 3.99TAWVAAAGEE106 pKa = 4.18QQSVVLLAAPVQHH119 pKa = 6.96PSLYY123 pKa = 10.38AYY125 pKa = 10.06AVEE128 pKa = 4.52AGAGFALLVPLVVII142 pKa = 5.08

Molecular weight: 15.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M9YCN0|A0A0M9YCN0_9ACTN Membrane protein OS=Streptomyces sp. WM4235 OX=1415551 GN=ADK55_31555 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.34GRR40 pKa = 11.84AALSAA45 pKa = 3.96

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6275

0

6275

1929375

25

3602

307.5

32.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.808 ± 0.051

0.782 ± 0.009

5.91 ± 0.027

5.761 ± 0.031

2.726 ± 0.02

9.52 ± 0.034

2.278 ± 0.015

3.236 ± 0.022

2.193 ± 0.023

10.376 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.769 ± 0.013

1.79 ± 0.017

6.171 ± 0.03

2.618 ± 0.017

8.017 ± 0.036

4.979 ± 0.021

6.077 ± 0.03

8.438 ± 0.028

1.528 ± 0.012

2.023 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski