Amino acid dipepetide frequency for Streptomyces sp. WM4235

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.742AlaAla: 21.742 ± 0.193	1.076AlaCys: 1.076 ± 0.027	8.459AlaAsp: 8.459 ± 0.064	8.96AlaGlu: 8.96 ± 0.096	3.594AlaPhe: 3.594 ± 0.043	13.799AlaGly: 13.799 ± 0.094	2.947AlaHis: 2.947 ± 0.036	3.512AlaIle: 3.512 ± 0.05	3.006AlaLys: 3.006 ± 0.05	14.88AlaLeu: 14.88 ± 0.114	2.644AlaMet: 2.644 ± 0.043	2.001AlaAsn: 2.001 ± 0.039	7.465AlaPro: 7.465 ± 0.072	3.591AlaGln: 3.591 ± 0.044	10.517AlaArg: 10.517 ± 0.09	5.934AlaSer: 5.934 ± 0.061	7.069AlaThr: 7.069 ± 0.064	12.249AlaVal: 12.249 ± 0.091	1.906AlaTrp: 1.906 ± 0.036	2.726AlaTyr: 2.726 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	1.148CysAla: 1.148 ± 0.027	0.09CysCys: 0.09 ± 0.008	0.468CysAsp: 0.468 ± 0.018	0.409CysGlu: 0.409 ± 0.017	0.221CysPhe: 0.221 ± 0.011	0.948CysGly: 0.948 ± 0.021	0.192CysHis: 0.192 ± 0.01	0.146CysIle: 0.146 ± 0.008	0.117CysLys: 0.117 ± 0.007	0.766CysLeu: 0.766 ± 0.02	0.134CysMet: 0.134 ± 0.009	0.138CysAsn: 0.138 ± 0.009	0.486CysPro: 0.486 ± 0.018	0.16CysGln: 0.16 ± 0.009	0.582CysArg: 0.582 ± 0.019	0.381CysSer: 0.381 ± 0.015	0.476CysThr: 0.476 ± 0.017	0.689CysVal: 0.689 ± 0.02	0.126CysTrp: 0.126 ± 0.009	0.144CysTyr: 0.144 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.606AspAla: 7.606 ± 0.074	0.456AspCys: 0.456 ± 0.013	3.209AspAsp: 3.209 ± 0.046	3.768AspGlu: 3.768 ± 0.053	1.595AspPhe: 1.595 ± 0.031	6.224AspGly: 6.224 ± 0.069	1.475AspHis: 1.475 ± 0.032	1.832AspIle: 1.832 ± 0.034	1.226AspLys: 1.226 ± 0.038	6.306AspLeu: 6.306 ± 0.067	0.845AspMet: 0.845 ± 0.023	0.966AspAsn: 0.966 ± 0.026	4.732AspPro: 4.732 ± 0.058	1.446AspGln: 1.446 ± 0.038	5.009AspArg: 5.009 ± 0.059	2.498AspSer: 2.498 ± 0.037	3.237AspThr: 3.237 ± 0.04	4.586AspVal: 4.586 ± 0.053	1.045AspTrp: 1.045 ± 0.024	1.035AspTyr: 1.035 ± 0.026	0.0AspXaa: 0.0 ± 0.0
Glu	7.77GluAla: 7.77 ± 0.088	0.345GluCys: 0.345 ± 0.014	2.857GluAsp: 2.857 ± 0.042	3.622GluGlu: 3.622 ± 0.053	1.53GluPhe: 1.53 ± 0.03	4.443GluGly: 4.443 ± 0.049	1.486GluHis: 1.486 ± 0.031	2.329GluIle: 2.329 ± 0.038	1.403GluLys: 1.403 ± 0.036	6.944GluLeu: 6.944 ± 0.07	0.894GluMet: 0.894 ± 0.025	1.061GluAsn: 1.061 ± 0.023	3.353GluPro: 3.353 ± 0.048	2.098GluGln: 2.098 ± 0.036	5.461GluArg: 5.461 ± 0.061	2.637GluSer: 2.637 ± 0.034	2.844GluThr: 2.844 ± 0.045	4.599GluVal: 4.599 ± 0.057	0.772GluTrp: 0.772 ± 0.02	1.166GluTyr: 1.166 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	3.684PheAla: 3.684 ± 0.052	0.278PheCys: 0.278 ± 0.012	1.95PheAsp: 1.95 ± 0.036	1.462PheGlu: 1.462 ± 0.03	0.851PhePhe: 0.851 ± 0.022	3.007PheGly: 3.007 ± 0.042	0.642PheHis: 0.642 ± 0.017	0.757PheIle: 0.757 ± 0.022	0.554PheLys: 0.554 ± 0.018	2.612PheLeu: 2.612 ± 0.041	0.415PheMet: 0.415 ± 0.016	0.594PheAsn: 0.594 ± 0.02	1.415PhePro: 1.415 ± 0.031	0.643PheGln: 0.643 ± 0.019	1.822PheArg: 1.822 ± 0.029	1.437PheSer: 1.437 ± 0.025	1.995PheThr: 1.995 ± 0.032	2.196PheVal: 2.196 ± 0.032	0.405PheTrp: 0.405 ± 0.015	0.538PheTyr: 0.538 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	11.485GlyAla: 11.485 ± 0.093	0.834GlyCys: 0.834 ± 0.025	5.077GlyAsp: 5.077 ± 0.059	5.163GlyGlu: 5.163 ± 0.05	2.898GlyPhe: 2.898 ± 0.041	8.931GlyGly: 8.931 ± 0.098	2.305GlyHis: 2.305 ± 0.036	3.673GlyIle: 3.673 ± 0.051	2.54GlyLys: 2.54 ± 0.044	9.595GlyLeu: 9.595 ± 0.097	1.977GlyMet: 1.977 ± 0.033	1.802GlyAsn: 1.802 ± 0.035	5.43GlyPro: 5.43 ± 0.061	2.618GlyGln: 2.618 ± 0.041	7.93GlyArg: 7.93 ± 0.069	5.407GlySer: 5.407 ± 0.062	6.043GlyThr: 6.043 ± 0.059	7.464GlyVal: 7.464 ± 0.065	1.739GlyTrp: 1.739 ± 0.032	2.289GlyTyr: 2.289 ± 0.038	0.0GlyXaa: 0.0 ± 0.0
His	2.757HisAla: 2.757 ± 0.038	0.215HisCys: 0.215 ± 0.012	1.359HisAsp: 1.359 ± 0.027	1.207HisGlu: 1.207 ± 0.025	0.629HisPhe: 0.629 ± 0.019	2.34HisGly: 2.34 ± 0.035	0.705HisHis: 0.705 ± 0.022	0.632HisIle: 0.632 ± 0.019	0.388HisLys: 0.388 ± 0.014	2.414HisLeu: 2.414 ± 0.037	0.357HisMet: 0.357 ± 0.013	0.351HisAsn: 0.351 ± 0.013	1.864HisPro: 1.864 ± 0.034	0.619HisGln: 0.619 ± 0.019	2.038HisArg: 2.038 ± 0.033	1.004HisSer: 1.004 ± 0.025	1.392HisThr: 1.392 ± 0.027	1.694HisVal: 1.694 ± 0.028	0.376HisTrp: 0.376 ± 0.014	0.437HisTyr: 0.437 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	4.978IleAla: 4.978 ± 0.068	0.297IleCys: 0.297 ± 0.013	2.205IleAsp: 2.205 ± 0.039	2.091IleGlu: 2.091 ± 0.029	0.713IlePhe: 0.713 ± 0.022	3.717IleGly: 3.717 ± 0.06	0.639IleHis: 0.639 ± 0.02	0.851IleIle: 0.851 ± 0.025	0.725IleLys: 0.725 ± 0.022	2.465IleLeu: 2.465 ± 0.04	0.434IleMet: 0.434 ± 0.015	0.706IleAsn: 0.706 ± 0.02	1.821IlePro: 1.821 ± 0.032	0.728IleGln: 0.728 ± 0.021	2.405IleArg: 2.405 ± 0.038	1.701IleSer: 1.701 ± 0.029	2.27IleThr: 2.27 ± 0.036	2.756IleVal: 2.756 ± 0.043	0.388IleTrp: 0.388 ± 0.013	0.474IleTyr: 0.474 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	3.156LysAla: 3.156 ± 0.059	0.118LysCys: 0.118 ± 0.008	1.372LysAsp: 1.372 ± 0.035	1.207LysGlu: 1.207 ± 0.028	0.478LysPhe: 0.478 ± 0.016	1.878LysGly: 1.878 ± 0.036	0.428LysHis: 0.428 ± 0.015	0.917LysIle: 0.917 ± 0.026	0.803LysLys: 0.803 ± 0.032	1.993LysLeu: 1.993 ± 0.039	0.402LysMet: 0.402 ± 0.017	0.546LysAsn: 0.546 ± 0.019	1.422LysPro: 1.422 ± 0.034	0.686LysGln: 0.686 ± 0.024	1.421LysArg: 1.421 ± 0.029	1.14LysSer: 1.14 ± 0.03	1.304LysThr: 1.304 ± 0.029	1.948LysVal: 1.948 ± 0.038	0.272LysTrp: 0.272 ± 0.01	0.442LysTyr: 0.442 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	15.386LeuAla: 15.386 ± 0.115	0.867LeuCys: 0.867 ± 0.025	6.739LeuAsp: 6.739 ± 0.066	4.769LeuGlu: 4.769 ± 0.058	2.616LeuPhe: 2.616 ± 0.045	9.396LeuGly: 9.396 ± 0.086	2.247LeuHis: 2.247 ± 0.037	3.38LeuIle: 3.38 ± 0.048	1.982LeuLys: 1.982 ± 0.036	11.378LeuLeu: 11.378 ± 0.108	1.643LeuMet: 1.643 ± 0.028	1.708LeuAsn: 1.708 ± 0.034	6.583LeuPro: 6.583 ± 0.068	2.09LeuGln: 2.09 ± 0.034	8.68LeuArg: 8.68 ± 0.083	5.236LeuSer: 5.236 ± 0.056	6.967LeuThr: 6.967 ± 0.068	8.913LeuVal: 8.913 ± 0.076	1.333LeuTrp: 1.333 ± 0.027	1.853LeuTyr: 1.853 ± 0.029	0.0LeuXaa: 0.0 ± 0.0
Met	2.341MetAla: 2.341 ± 0.041	0.135MetCys: 0.135 ± 0.008	0.927MetAsp: 0.927 ± 0.024	0.785MetGlu: 0.785 ± 0.021	0.458MetPhe: 0.458 ± 0.016	1.363MetGly: 1.363 ± 0.03	0.338MetHis: 0.338 ± 0.013	0.701MetIle: 0.701 ± 0.02	0.418MetLys: 0.418 ± 0.016	1.786MetLeu: 1.786 ± 0.03	0.297MetMet: 0.297 ± 0.013	0.45MetAsn: 0.45 ± 0.014	1.168MetPro: 1.168 ± 0.026	0.414MetGln: 0.414 ± 0.016	1.475MetArg: 1.475 ± 0.031	1.242MetSer: 1.242 ± 0.026	1.462MetThr: 1.462 ± 0.028	1.36MetVal: 1.36 ± 0.026	0.237MetTrp: 0.237 ± 0.011	0.334MetTyr: 0.334 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.289AsnAla: 2.289 ± 0.04	0.169AsnCys: 0.169 ± 0.01	0.935AsnAsp: 0.935 ± 0.022	0.858AsnGlu: 0.858 ± 0.021	0.503AsnPhe: 0.503 ± 0.015	1.868AsnGly: 1.868 ± 0.044	0.402AsnHis: 0.402 ± 0.015	0.647AsnIle: 0.647 ± 0.02	0.448AsnLys: 0.448 ± 0.017	1.7AsnLeu: 1.7 ± 0.034	0.317AsnMet: 0.317 ± 0.012	0.426AsnAsn: 0.426 ± 0.017	1.42AsnPro: 1.42 ± 0.028	0.523AsnGln: 0.523 ± 0.019	1.289AsnArg: 1.289 ± 0.03	0.915AsnSer: 0.915 ± 0.021	1.191AsnThr: 1.191 ± 0.027	1.368AsnVal: 1.368 ± 0.032	0.278AsnTrp: 0.278 ± 0.014	0.354AsnTyr: 0.354 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	9.144ProAla: 9.144 ± 0.092	0.325ProCys: 0.325 ± 0.012	4.502ProAsp: 4.502 ± 0.055	4.358ProGlu: 4.358 ± 0.051	1.582ProPhe: 1.582 ± 0.033	6.772ProGly: 6.772 ± 0.079	1.34ProHis: 1.34 ± 0.024	1.377ProIle: 1.377 ± 0.026	1.221ProLys: 1.221 ± 0.031	5.429ProLeu: 5.429 ± 0.065	1.025ProMet: 1.025 ± 0.023	0.963ProAsn: 0.963 ± 0.02	3.481ProPro: 3.481 ± 0.062	1.697ProGln: 1.697 ± 0.041	4.045ProArg: 4.045 ± 0.059	3.14ProSer: 3.14 ± 0.045	3.316ProThr: 3.316 ± 0.04	5.669ProVal: 5.669 ± 0.058	0.909ProTrp: 0.909 ± 0.02	1.413ProTyr: 1.413 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	3.673GlnAla: 3.673 ± 0.054	0.181GlnCys: 0.181 ± 0.011	1.355GlnAsp: 1.355 ± 0.028	1.396GlnGlu: 1.396 ± 0.029	0.628GlnPhe: 0.628 ± 0.016	2.295GlnGly: 2.295 ± 0.034	0.562GlnHis: 0.562 ± 0.015	1.064GlnIle: 1.064 ± 0.023	0.594GlnLys: 0.594 ± 0.019	2.773GlnLeu: 2.773 ± 0.041	0.488GlnMet: 0.488 ± 0.015	0.513GlnAsn: 0.513 ± 0.016	1.531GlnPro: 1.531 ± 0.034	1.122GlnGln: 1.122 ± 0.04	2.161GlnArg: 2.161 ± 0.038	1.273GlnSer: 1.273 ± 0.027	1.247GlnThr: 1.247 ± 0.023	2.266GlnVal: 2.266 ± 0.038	0.457GlnTrp: 0.457 ± 0.017	0.598GlnTyr: 0.598 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	9.957ArgAla: 9.957 ± 0.087	0.534ArgCys: 0.534 ± 0.018	4.287ArgAsp: 4.287 ± 0.05	4.904ArgGlu: 4.904 ± 0.054	2.326ArgPhe: 2.326 ± 0.036	5.96ArgGly: 5.96 ± 0.059	2.129ArgHis: 2.129 ± 0.032	3.376ArgIle: 3.376 ± 0.042	1.622ArgLys: 1.622 ± 0.03	8.894ArgLeu: 8.894 ± 0.084	1.717ArgMet: 1.717 ± 0.028	1.34ArgAsn: 1.34 ± 0.028	4.968ArgPro: 4.968 ± 0.058	2.17ArgGln: 2.17 ± 0.036	7.503ArgArg: 7.503 ± 0.078	4.025ArgSer: 4.025 ± 0.049	5.341ArgThr: 5.341 ± 0.057	6.039ArgVal: 6.039 ± 0.064	1.375ArgTrp: 1.375 ± 0.032	1.701ArgTyr: 1.701 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	6.778SerAla: 6.778 ± 0.065	0.398SerCys: 0.398 ± 0.015	2.632SerAsp: 2.632 ± 0.04	2.473SerGlu: 2.473 ± 0.039	1.53SerPhe: 1.53 ± 0.029	5.872SerGly: 5.872 ± 0.065	1.017SerHis: 1.017 ± 0.025	1.435SerIle: 1.435 ± 0.03	1.014SerLys: 1.014 ± 0.024	4.795SerLeu: 4.795 ± 0.061	1.059SerMet: 1.059 ± 0.027	0.841SerAsn: 0.841 ± 0.022	3.153SerPro: 3.153 ± 0.045	1.19SerGln: 1.19 ± 0.025	3.655SerArg: 3.655 ± 0.05	2.607SerSer: 2.607 ± 0.05	3.021SerThr: 3.021 ± 0.045	4.271SerVal: 4.271 ± 0.047	0.888SerTrp: 0.888 ± 0.021	1.163SerTyr: 1.163 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	8.949ThrAla: 8.949 ± 0.063	0.43ThrCys: 0.43 ± 0.016	3.594ThrAsp: 3.594 ± 0.048	3.196ThrGlu: 3.196 ± 0.04	1.553ThrPhe: 1.553 ± 0.031	6.667ThrGly: 6.667 ± 0.064	1.235ThrHis: 1.235 ± 0.025	1.725ThrIle: 1.725 ± 0.033	1.176ThrLys: 1.176 ± 0.032	5.864ThrLeu: 5.864 ± 0.052	0.956ThrMet: 0.956 ± 0.021	0.984ThrAsn: 0.984 ± 0.025	4.299ThrPro: 4.299 ± 0.055	1.228ThrGln: 1.228 ± 0.026	3.999ThrArg: 3.999 ± 0.039	2.977ThrSer: 2.977 ± 0.045	3.825ThrThr: 3.825 ± 0.059	5.912ThrVal: 5.912 ± 0.058	0.923ThrTrp: 0.923 ± 0.024	1.275ThrTyr: 1.275 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	10.733ValAla: 10.733 ± 0.096	0.739ValCys: 0.739 ± 0.019	4.971ValAsp: 4.971 ± 0.054	4.937ValGlu: 4.937 ± 0.049	2.41ValPhe: 2.41 ± 0.037	6.472ValGly: 6.472 ± 0.069	1.961ValHis: 1.961 ± 0.029	2.942ValIle: 2.942 ± 0.046	1.88ValLys: 1.88 ± 0.036	9.403ValLeu: 9.403 ± 0.082	1.52ValMet: 1.52 ± 0.03	1.718ValAsn: 1.718 ± 0.03	5.267ValPro: 5.267 ± 0.063	1.969ValGln: 1.969 ± 0.033	7.067ValArg: 7.067 ± 0.074	4.362ValSer: 4.362 ± 0.055	5.568ValThr: 5.568 ± 0.06	7.78ValVal: 7.78 ± 0.078	1.162ValTrp: 1.162 ± 0.024	1.524ValTyr: 1.524 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	1.729TrpAla: 1.729 ± 0.031	0.152TrpCys: 0.152 ± 0.008	0.824TrpAsp: 0.824 ± 0.024	0.745TrpGlu: 0.745 ± 0.016	0.512TrpPhe: 0.512 ± 0.016	1.057TrpGly: 1.057 ± 0.026	0.362TrpHis: 0.362 ± 0.014	0.611TrpIle: 0.611 ± 0.017	0.404TrpLys: 0.404 ± 0.015	1.743TrpLeu: 1.743 ± 0.035	0.308TrpMet: 0.308 ± 0.013	0.412TrpAsn: 0.412 ± 0.017	0.805TrpPro: 0.805 ± 0.022	0.592TrpGln: 0.592 ± 0.019	1.3TrpArg: 1.3 ± 0.026	0.949TrpSer: 0.949 ± 0.024	1.072TrpThr: 1.072 ± 0.025	1.003TrpVal: 1.003 ± 0.025	0.344TrpTrp: 0.344 ± 0.015	0.355TrpTyr: 0.355 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.772TyrAla: 2.772 ± 0.04	0.183TyrCys: 0.183 ± 0.01	1.372TyrAsp: 1.372 ± 0.032	1.304TyrGlu: 1.304 ± 0.028	0.629TyrPhe: 0.629 ± 0.019	2.19TyrGly: 2.19 ± 0.036	0.368TyrHis: 0.368 ± 0.014	0.456TyrIle: 0.456 ± 0.015	0.414TyrLys: 0.414 ± 0.014	2.027TyrLeu: 2.027 ± 0.034	0.26TyrMet: 0.26 ± 0.014	0.379TyrAsn: 0.379 ± 0.014	1.046TyrPro: 1.046 ± 0.026	0.591TyrGln: 0.591 ± 0.019	1.81TyrArg: 1.81 ± 0.031	0.927TyrSer: 0.927 ± 0.026	1.127TyrThr: 1.127 ± 0.026	1.621TyrVal: 1.621 ± 0.029	0.344TyrTrp: 0.344 ± 0.015	0.408TyrTyr: 0.408 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6275 proteins (1929375 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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