Paenibacillus sp. 1_12

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6757 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4IWZ2|A0A1I4IWZ2_9BACL Arylamine N-acetyltransferase OS=Paenibacillus sp. 1_12 OX=1566278 GN=SAMN03159341_107256 PE=3 SV=1
MM1 pKa = 6.83SQSYY5 pKa = 9.98LPNISPLNTLTSDD18 pKa = 3.62DD19 pKa = 5.06AINLLVSSTALEE31 pKa = 4.07EE32 pKa = 4.25LGLRR36 pKa = 11.84QLIHH40 pKa = 6.55VDD42 pKa = 3.28SVKK45 pKa = 10.58PISVWPAEE53 pKa = 4.0AMDD56 pKa = 4.13IEE58 pKa = 5.5AIGDD62 pKa = 3.78PDD64 pKa = 3.5VTEE67 pKa = 4.03IAGASLPSAVNLIYY81 pKa = 10.54VANAGNDD88 pKa = 3.45NVSVIDD94 pKa = 4.02GNTNTVIATVAVGSIPFGVGVNSNTNRR121 pKa = 11.84IYY123 pKa = 10.79VANLVSNNVSVIDD136 pKa = 4.97GITNTVIATVTVGNSPFGVGVNPNTNLIYY165 pKa = 10.53AVNLSGSNVSVISGLSNTVIATVAVGIGPQGIEE198 pKa = 3.99VNPITNRR205 pKa = 11.84IYY207 pKa = 10.85VANSTNNNVSVISGITNTVVATVSVGFEE235 pKa = 3.76PVGIGINPIDD245 pKa = 3.57NRR247 pKa = 11.84IYY249 pKa = 9.47VTNSASNNVSVISGLSNTVIATVTVGNDD277 pKa = 3.27PEE279 pKa = 4.58FGVAVNPNTNLIYY292 pKa = 10.6VVNINSGNVSVIDD305 pKa = 3.99GNANTVISTIPVGSQPYY322 pKa = 9.1DD323 pKa = 3.4VSVNSLTNGIYY334 pKa = 10.36VGNNGGNNTSVIDD347 pKa = 4.05GNTNTIIATFAVGNGPTGVKK367 pKa = 10.12VNPP370 pKa = 4.06

Molecular weight: 37.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4M8E0|A0A1I4M8E0_9BACL Uncharacterized protein OS=Paenibacillus sp. 1_12 OX=1566278 GN=SAMN03159341_113125 PE=4 SV=1
MM1 pKa = 7.61GPTFKK6 pKa = 10.88PNTRR10 pKa = 11.84KK11 pKa = 9.83RR12 pKa = 11.84KK13 pKa = 8.74KK14 pKa = 8.74VHH16 pKa = 5.61GFRR19 pKa = 11.84KK20 pKa = 9.95RR21 pKa = 11.84MSSVNGRR28 pKa = 11.84KK29 pKa = 9.15VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6757

0

6757

2176029

39

3024

322.0

36.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.569 ± 0.035

0.786 ± 0.009

5.085 ± 0.021

6.338 ± 0.038

4.222 ± 0.021

6.978 ± 0.03

2.158 ± 0.016

7.249 ± 0.028

5.741 ± 0.025

10.082 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.871 ± 0.014

4.212 ± 0.025

3.924 ± 0.018

4.198 ± 0.021

4.506 ± 0.026

6.664 ± 0.027

5.646 ± 0.033

6.915 ± 0.024

1.289 ± 0.012

3.57 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski