Riesia pediculicola (strain USDA)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Candidatus Riesia; Candidatus Riesia pediculicola

Average proteome isoelectric point is 8.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 540 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4G8V5|D4G8V5_RIEPU Glutamate dehydrogenase OS=Riesia pediculicola (strain USDA) OX=515618 GN=RIEPE_0531 PE=3 SV=1
MM1 pKa = 8.2DD2 pKa = 4.54NFLDD6 pKa = 3.86YY7 pKa = 11.02RR8 pKa = 11.84LGLINIEE15 pKa = 4.01RR16 pKa = 11.84KK17 pKa = 8.03MQQEE21 pKa = 3.91DD22 pKa = 3.58HH23 pKa = 6.91LSIFNQIEE31 pKa = 4.73RR32 pKa = 11.84YY33 pKa = 9.81CLDD36 pKa = 3.14INFIIIEE43 pKa = 4.0QFIEE47 pKa = 3.97INNEE51 pKa = 4.02FIFSII56 pKa = 4.34

Molecular weight: 6.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4G7W4|D4G7W4_RIEPU Ribonuclease P protein component OS=Riesia pediculicola (strain USDA) OX=515618 GN=rnpA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.53RR3 pKa = 11.84TFQPSILKK11 pKa = 10.23RR12 pKa = 11.84LRR14 pKa = 11.84THH16 pKa = 6.47GFRR19 pKa = 11.84SRR21 pKa = 11.84MSKK24 pKa = 10.11KK25 pKa = 9.43SGRR28 pKa = 11.84IILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.73RR38 pKa = 11.84RR39 pKa = 11.84VKK41 pKa = 9.66ITVSSEE47 pKa = 3.92KK48 pKa = 10.23KK49 pKa = 10.26KK50 pKa = 10.78KK51 pKa = 10.17

Molecular weight: 6.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

540

0

540

152452

19

1392

282.3

32.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.276 ± 0.083

1.26 ± 0.031

4.304 ± 0.071

5.905 ± 0.11

5.819 ± 0.108

5.419 ± 0.102

1.967 ± 0.042

11.49 ± 0.118

10.514 ± 0.117

9.309 ± 0.094

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.342 ± 0.04

5.948 ± 0.072

2.725 ± 0.044

3.134 ± 0.055

4.967 ± 0.076

8.296 ± 0.081

3.811 ± 0.058

5.133 ± 0.075

0.828 ± 0.031

3.551 ± 0.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski