Chlorobium phaeovibrioides (strain DSM 265 / 1930) (Prosthecochloris vibrioformis (strain DSM 265))
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1750 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A4SGE4|A4SGE4_CHLPM ABC transporter related protein OS=Chlorobium phaeovibrioides (strain DSM 265 / 1930) OX=290318 GN=Cvib_1542 PE=4 SV=1
MM1 pKa = 7.39 KK2 pKa = 10.39 KK3 pKa = 10.22 KK4 pKa = 10.4 LALLTAGLALAGFSAAPASAADD26 pKa = 4.22 HH27 pKa = 5.72 YY28 pKa = 11.57 VSGNAGITWMNDD40 pKa = 2.11 VDD42 pKa = 4.41 YY43 pKa = 10.55 AGHH46 pKa = 6.48 KK47 pKa = 10.86 AEE49 pKa = 4.38 MDD51 pKa = 3.06 AGFALFGAIGCDD63 pKa = 3.48 YY64 pKa = 11.3 GDD66 pKa = 3.4 TRR68 pKa = 11.84 LEE70 pKa = 4.2 GEE72 pKa = 4.53 LGYY75 pKa = 8.79 MQNDD79 pKa = 3.22 VDD81 pKa = 4.44 KK82 pKa = 11.41 VDD84 pKa = 3.77 GASASGDD91 pKa = 2.98 ISVISLMANGYY102 pKa = 10.23 YY103 pKa = 10.44 DD104 pKa = 3.85 IDD106 pKa = 3.63 TGGGIEE112 pKa = 4.28 PYY114 pKa = 8.72 ITAGVGFAQGHH125 pKa = 6.12 AEE127 pKa = 3.95 DD128 pKa = 4.01 VTNFGTWNEE137 pKa = 4.18 TTLAWQVGAGVAVPVGNNVMVDD159 pKa = 2.87 LRR161 pKa = 11.84 YY162 pKa = 10.05 RR163 pKa = 11.84 YY164 pKa = 9.89 FDD166 pKa = 3.55 TTDD169 pKa = 3.35 MLDD172 pKa = 3.58 DD173 pKa = 4.05 TNIGSNNLLVGMRR186 pKa = 11.84 VGFF189 pKa = 3.93
Molecular weight: 19.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.789
IPC2_protein 4.012
IPC_protein 3.999
Toseland 3.77
ProMoST 4.177
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.961
Rodwell 3.821
Grimsley 3.681
Solomon 3.999
Lehninger 3.961
Nozaki 4.126
DTASelect 4.406
Thurlkill 3.834
EMBOSS 3.973
Sillero 4.126
Patrickios 1.952
IPC_peptide 3.986
IPC2_peptide 4.101
IPC2.peptide.svr19 4.014
Protein with the highest isoelectric point:
>tr|A4SC39|A4SC39_CHLPM Uncharacterized protein OS=Chlorobium phaeovibrioides (strain DSM 265 / 1930) OX=290318 GN=Cvib_0019 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 KK11 pKa = 8.89 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 10.06 HH16 pKa = 3.77 GFRR19 pKa = 11.84 LRR21 pKa = 11.84 MSTKK25 pKa = 9.28 NGRR28 pKa = 11.84 RR29 pKa = 11.84 IINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.92 GRR39 pKa = 11.84 HH40 pKa = 4.96 SLSVSSDD47 pKa = 2.66 MGTAGRR53 pKa = 4.29
Molecular weight: 6.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.441
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.369
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.102
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1750
0
1750
594863
36
7428
339.9
37.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.267 ± 0.076
1.01 ± 0.03
5.201 ± 0.115
6.862 ± 0.067
4.232 ± 0.055
7.983 ± 0.061
2.085 ± 0.038
6.157 ± 0.048
4.664 ± 0.067
10.198 ± 0.083
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.754 ± 0.042
3.302 ± 0.039
4.422 ± 0.057
3.027 ± 0.032
5.961 ± 0.088
6.845 ± 0.061
5.324 ± 0.133
6.917 ± 0.057
1.015 ± 0.021
2.773 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here