Lactococcus phage Q33

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vedamuthuvirus; Lactococcus virus Q33

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9QN76|R9QN76_9CAUD Terminase large subunit OS=Lactococcus phage Q33 OX=254253 GN=Q33_0032 PE=4 SV=1
MM1 pKa = 7.23KK2 pKa = 10.11KK3 pKa = 8.64YY4 pKa = 9.83WVVEE8 pKa = 3.9DD9 pKa = 3.74HH10 pKa = 6.88LGGGFYY16 pKa = 10.8LMPEE20 pKa = 4.1DD21 pKa = 4.04TPEE24 pKa = 3.89EE25 pKa = 3.99EE26 pKa = 4.05LRR28 pKa = 11.84EE29 pKa = 4.1VEE31 pKa = 4.58VQCDD35 pKa = 3.38TCGDD39 pKa = 3.53NDD41 pKa = 4.7SIIGQFSNWNQLKK54 pKa = 10.45KK55 pKa = 11.08EE56 pKa = 4.06MTDD59 pKa = 3.5DD60 pKa = 4.56EE61 pKa = 5.2GWCPYY66 pKa = 10.28SDD68 pKa = 5.14EE69 pKa = 4.2YY70 pKa = 10.46LQSVFEE76 pKa = 4.68EE77 pKa = 4.75DD78 pKa = 3.42NQQ80 pKa = 3.87

Molecular weight: 9.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9QNF7|R9QNF7_9CAUD Uncharacterized protein OS=Lactococcus phage Q33 OX=254253 GN=Q33_004 PE=4 SV=1
MM1 pKa = 7.72GEE3 pKa = 4.12IKK5 pKa = 10.51RR6 pKa = 11.84LFPKK10 pKa = 10.15RR11 pKa = 11.84GRR13 pKa = 11.84FFLCYY18 pKa = 9.79NIVGMFVRR26 pKa = 11.84FHH28 pKa = 7.12PIPRR32 pKa = 11.84VKK34 pKa = 10.41PFFGVAFFVCCKK46 pKa = 8.66FTTILSEE53 pKa = 4.18TT54 pKa = 3.81

Molecular weight: 6.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

9026

36

759

180.5

20.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.847 ± 0.541

0.632 ± 0.112

6.215 ± 0.385

7.113 ± 0.514

4.83 ± 0.263

6.714 ± 0.483

1.252 ± 0.203

7.046 ± 0.376

8.941 ± 0.409

7.589 ± 0.298

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.371 ± 0.14

6.16 ± 0.268

2.659 ± 0.164

4.409 ± 0.262

3.933 ± 0.268

6.071 ± 0.298

6.503 ± 0.363

5.994 ± 0.267

1.119 ± 0.142

3.601 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski