Amino acid dipepetide frequency for Lactococcus phage Q33

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.657AlaAla: 3.657 ± 0.691
0.332AlaCys: 0.332 ± 0.257
4.543AlaAsp: 4.543 ± 0.855
3.989AlaGlu: 3.989 ± 0.769
3.657AlaPhe: 3.657 ± 0.676
4.875AlaGly: 4.875 ± 1.155
0.776AlaHis: 0.776 ± 0.277
5.651AlaIle: 5.651 ± 1.151
5.429AlaLys: 5.429 ± 0.824
4.543AlaLeu: 4.543 ± 0.594
2.77AlaMet: 2.77 ± 0.656
4.1AlaAsn: 4.1 ± 0.638
1.662AlaPro: 1.662 ± 0.429
3.213AlaGln: 3.213 ± 0.544
2.548AlaArg: 2.548 ± 0.472
4.1AlaSer: 4.1 ± 0.587
5.097AlaThr: 5.097 ± 1.495
4.211AlaVal: 4.211 ± 0.814
0.997AlaTrp: 0.997 ± 0.272
2.327AlaTyr: 2.327 ± 0.418
0.0AlaXaa: 0.0 ± 0.0
Cys
0.0CysAla: 0.0 ± 0.0
0.111CysCys: 0.111 ± 0.122
0.443CysAsp: 0.443 ± 0.253
0.886CysGlu: 0.886 ± 0.367
0.443CysPhe: 0.443 ± 0.263
0.554CysGly: 0.554 ± 0.259
0.111CysHis: 0.111 ± 0.099
0.222CysIle: 0.222 ± 0.142
0.332CysLys: 0.332 ± 0.218
0.554CysLeu: 0.554 ± 0.265
0.0CysMet: 0.0 ± 0.0
0.554CysAsn: 0.554 ± 0.286
0.222CysPro: 0.222 ± 0.159
0.0CysGln: 0.0 ± 0.0
0.332CysArg: 0.332 ± 0.192
0.554CysSer: 0.554 ± 0.296
0.443CysThr: 0.443 ± 0.217
0.222CysVal: 0.222 ± 0.152
0.0CysTrp: 0.0 ± 0.0
0.332CysTyr: 0.332 ± 0.18
0.0CysXaa: 0.0 ± 0.0
Asp
3.546AspAla: 3.546 ± 0.788
0.554AspCys: 0.554 ± 0.286
4.321AspAsp: 4.321 ± 1.025
4.986AspGlu: 4.986 ± 0.732
3.102AspPhe: 3.102 ± 0.537
5.097AspGly: 5.097 ± 0.965
0.776AspHis: 0.776 ± 0.302
4.432AspIle: 4.432 ± 0.583
5.762AspLys: 5.762 ± 0.966
5.429AspLeu: 5.429 ± 0.855
1.662AspMet: 1.662 ± 0.388
4.321AspAsn: 4.321 ± 0.613
1.884AspPro: 1.884 ± 0.636
0.997AspGln: 0.997 ± 0.415
2.105AspArg: 2.105 ± 0.559
4.432AspSer: 4.432 ± 0.715
2.659AspThr: 2.659 ± 0.506
2.438AspVal: 2.438 ± 0.559
0.886AspTrp: 0.886 ± 0.285
2.77AspTyr: 2.77 ± 0.541
0.0AspXaa: 0.0 ± 0.0
Glu
4.321GluAla: 4.321 ± 0.582
0.665GluCys: 0.665 ± 0.406
3.435GluAsp: 3.435 ± 0.95
4.432GluGlu: 4.432 ± 1.04
4.543GluPhe: 4.543 ± 0.586
2.992GluGly: 2.992 ± 0.492
0.443GluHis: 0.443 ± 0.233
5.429GluIle: 5.429 ± 0.834
6.205GluLys: 6.205 ± 0.995
6.316GluLeu: 6.316 ± 0.93
1.884GluMet: 1.884 ± 0.536
4.432GluAsn: 4.432 ± 0.573
0.997GluPro: 0.997 ± 0.273
3.657GluGln: 3.657 ± 0.664
4.1GluArg: 4.1 ± 0.808
3.657GluSer: 3.657 ± 0.599
4.765GluThr: 4.765 ± 0.687
5.762GluVal: 5.762 ± 0.614
0.776GluTrp: 0.776 ± 0.281
2.327GluTyr: 2.327 ± 0.556
0.0GluXaa: 0.0 ± 0.0
Phe
2.77PheAla: 2.77 ± 0.537
0.222PheCys: 0.222 ± 0.144
3.324PheAsp: 3.324 ± 0.737
3.767PheGlu: 3.767 ± 0.67
3.435PhePhe: 3.435 ± 0.96
3.767PheGly: 3.767 ± 0.596
0.554PheHis: 0.554 ± 0.244
2.216PheIle: 2.216 ± 0.621
3.878PheLys: 3.878 ± 0.719
3.435PheLeu: 3.435 ± 0.602
1.44PheMet: 1.44 ± 0.359
3.435PheAsn: 3.435 ± 0.9
1.108PhePro: 1.108 ± 0.354
2.105PheGln: 2.105 ± 0.448
1.219PheArg: 1.219 ± 0.457
4.211PheSer: 4.211 ± 0.579
2.881PheThr: 2.881 ± 0.662
2.327PheVal: 2.327 ± 0.505
0.332PheTrp: 0.332 ± 0.17
1.884PheTyr: 1.884 ± 0.429
0.0PheXaa: 0.0 ± 0.0
Gly
3.546GlyAla: 3.546 ± 0.881
0.332GlyCys: 0.332 ± 0.204
2.992GlyAsp: 2.992 ± 0.607
5.429GlyGlu: 5.429 ± 0.867
5.097GlyPhe: 5.097 ± 0.871
5.54GlyGly: 5.54 ± 0.869
0.554GlyHis: 0.554 ± 0.249
5.651GlyIle: 5.651 ± 1.123
7.202GlyLys: 7.202 ± 0.743
4.321GlyLeu: 4.321 ± 0.611
1.108GlyMet: 1.108 ± 0.293
2.992GlyAsn: 2.992 ± 0.666
0.665GlyPro: 0.665 ± 0.255
2.438GlyGln: 2.438 ± 0.651
2.992GlyArg: 2.992 ± 0.519
4.432GlySer: 4.432 ± 1.002
5.429GlyThr: 5.429 ± 0.966
2.881GlyVal: 2.881 ± 0.359
0.886GlyTrp: 0.886 ± 0.301
2.659GlyTyr: 2.659 ± 0.521
0.0GlyXaa: 0.0 ± 0.0
His
1.219HisAla: 1.219 ± 0.448
0.111HisCys: 0.111 ± 0.128
0.554HisAsp: 0.554 ± 0.283
0.886HisGlu: 0.886 ± 0.29
0.443HisPhe: 0.443 ± 0.218
0.886HisGly: 0.886 ± 0.328
0.332HisHis: 0.332 ± 0.297
1.108HisIle: 1.108 ± 0.302
0.997HisLys: 0.997 ± 0.339
0.665HisLeu: 0.665 ± 0.291
0.222HisMet: 0.222 ± 0.156
0.997HisAsn: 0.997 ± 0.41
0.332HisPro: 0.332 ± 0.208
0.665HisGln: 0.665 ± 0.294
0.222HisArg: 0.222 ± 0.139
0.997HisSer: 0.997 ± 0.303
0.665HisThr: 0.665 ± 0.286
0.554HisVal: 0.554 ± 0.254
0.111HisTrp: 0.111 ± 0.124
0.554HisTyr: 0.554 ± 0.22
0.0HisXaa: 0.0 ± 0.0
Ile
6.981IleAla: 6.981 ± 0.808
0.443IleCys: 0.443 ± 0.221
4.765IleAsp: 4.765 ± 0.746
4.321IleGlu: 4.321 ± 0.791
2.438IlePhe: 2.438 ± 0.467
4.986IleGly: 4.986 ± 1.278
1.33IleHis: 1.33 ± 0.418
3.989IleIle: 3.989 ± 0.626
6.759IleLys: 6.759 ± 0.868
3.767IleLeu: 3.767 ± 0.626
1.219IleMet: 1.219 ± 0.357
4.211IleAsn: 4.211 ± 0.64
2.881IlePro: 2.881 ± 0.585
2.659IleGln: 2.659 ± 0.446
2.327IleArg: 2.327 ± 0.538
5.429IleSer: 5.429 ± 0.811
5.54IleThr: 5.54 ± 0.912
3.324IleVal: 3.324 ± 0.578
0.443IleTrp: 0.443 ± 0.238
2.659IleTyr: 2.659 ± 0.506
0.0IleXaa: 0.0 ± 0.0
Lys
6.759LysAla: 6.759 ± 0.827
0.443LysCys: 0.443 ± 0.236
5.651LysAsp: 5.651 ± 0.781
7.424LysGlu: 7.424 ± 1.149
2.992LysPhe: 2.992 ± 0.448
6.316LysGly: 6.316 ± 0.75
1.551LysHis: 1.551 ± 0.581
5.54LysIle: 5.54 ± 0.872
8.753LysLys: 8.753 ± 1.117
7.645LysLeu: 7.645 ± 0.976
2.992LysMet: 2.992 ± 0.461
5.319LysAsn: 5.319 ± 0.851
2.216LysPro: 2.216 ± 0.518
2.659LysGln: 2.659 ± 0.468
3.989LysArg: 3.989 ± 0.625
3.989LysSer: 3.989 ± 0.509
5.208LysThr: 5.208 ± 0.628
5.208LysVal: 5.208 ± 0.94
1.33LysTrp: 1.33 ± 0.36
3.435LysTyr: 3.435 ± 0.724
0.0LysXaa: 0.0 ± 0.0
Leu
5.873LeuAla: 5.873 ± 0.671
0.332LeuCys: 0.332 ± 0.213
5.762LeuAsp: 5.762 ± 0.946
6.759LeuGlu: 6.759 ± 1.025
3.102LeuPhe: 3.102 ± 0.522
4.211LeuGly: 4.211 ± 0.647
0.776LeuHis: 0.776 ± 0.374
4.321LeuIle: 4.321 ± 0.691
6.316LeuLys: 6.316 ± 1.021
5.319LeuLeu: 5.319 ± 1.148
2.105LeuMet: 2.105 ± 0.515
5.319LeuAsn: 5.319 ± 0.671
1.551LeuPro: 1.551 ± 0.387
4.321LeuGln: 4.321 ± 0.739
2.659LeuArg: 2.659 ± 0.504
5.208LeuSer: 5.208 ± 0.743
5.762LeuThr: 5.762 ± 0.939
3.546LeuVal: 3.546 ± 0.588
0.776LeuTrp: 0.776 ± 0.291
1.884LeuTyr: 1.884 ± 0.397
0.0LeuXaa: 0.0 ± 0.0
Met
1.44MetAla: 1.44 ± 0.395
0.222MetCys: 0.222 ± 0.151
1.33MetAsp: 1.33 ± 0.365
1.994MetGlu: 1.994 ± 0.477
0.997MetPhe: 0.997 ± 0.413
0.886MetGly: 0.886 ± 0.434
0.222MetHis: 0.222 ± 0.152
1.662MetIle: 1.662 ± 0.399
2.659MetLys: 2.659 ± 0.595
1.773MetLeu: 1.773 ± 0.482
0.222MetMet: 0.222 ± 0.159
1.994MetAsn: 1.994 ± 0.442
1.108MetPro: 1.108 ± 0.309
1.33MetGln: 1.33 ± 0.37
0.886MetArg: 0.886 ± 0.295
0.997MetSer: 0.997 ± 0.438
2.105MetThr: 2.105 ± 0.393
1.551MetVal: 1.551 ± 0.365
0.111MetTrp: 0.111 ± 0.113
0.222MetTyr: 0.222 ± 0.157
0.0MetXaa: 0.0 ± 0.0
Asn
5.097AsnAla: 5.097 ± 1.201
0.554AsnCys: 0.554 ± 0.214
3.324AsnAsp: 3.324 ± 0.619
3.878AsnGlu: 3.878 ± 0.837
2.438AsnPhe: 2.438 ± 0.403
4.432AsnGly: 4.432 ± 0.718
0.886AsnHis: 0.886 ± 0.333
4.432AsnIle: 4.432 ± 0.465
5.208AsnLys: 5.208 ± 0.566
3.324AsnLeu: 3.324 ± 0.657
0.886AsnMet: 0.886 ± 0.291
4.986AsnAsn: 4.986 ± 0.854
2.327AsnPro: 2.327 ± 0.46
4.1AsnGln: 4.1 ± 0.761
1.994AsnArg: 1.994 ± 0.432
3.102AsnSer: 3.102 ± 0.504
3.213AsnThr: 3.213 ± 0.619
4.321AsnVal: 4.321 ± 0.591
0.886AsnTrp: 0.886 ± 0.364
2.105AsnTyr: 2.105 ± 0.402
0.0AsnXaa: 0.0 ± 0.0
Pro
1.108ProAla: 1.108 ± 0.341
0.222ProCys: 0.222 ± 0.158
2.548ProAsp: 2.548 ± 0.467
1.994ProGlu: 1.994 ± 0.533
1.551ProPhe: 1.551 ± 0.531
0.886ProGly: 0.886 ± 0.355
0.554ProHis: 0.554 ± 0.292
1.773ProIle: 1.773 ± 0.401
2.548ProLys: 2.548 ± 0.599
1.884ProLeu: 1.884 ± 0.398
0.665ProMet: 0.665 ± 0.23
0.997ProAsn: 0.997 ± 0.329
0.554ProPro: 0.554 ± 0.221
1.662ProGln: 1.662 ± 0.537
0.665ProArg: 0.665 ± 0.264
1.44ProSer: 1.44 ± 0.323
1.773ProThr: 1.773 ± 0.344
2.548ProVal: 2.548 ± 0.513
0.554ProTrp: 0.554 ± 0.262
0.665ProTyr: 0.665 ± 0.252
0.0ProXaa: 0.0 ± 0.0
Gln
3.546GlnAla: 3.546 ± 0.556
0.111GlnCys: 0.111 ± 0.134
1.44GlnAsp: 1.44 ± 0.447
3.102GlnGlu: 3.102 ± 0.501
2.216GlnPhe: 2.216 ± 0.499
2.327GlnGly: 2.327 ± 0.5
0.443GlnHis: 0.443 ± 0.189
3.102GlnIle: 3.102 ± 0.642
3.102GlnLys: 3.102 ± 0.491
4.765GlnLeu: 4.765 ± 0.756
1.662GlnMet: 1.662 ± 0.442
2.659GlnAsn: 2.659 ± 0.642
1.33GlnPro: 1.33 ± 0.411
2.548GlnGln: 2.548 ± 0.514
1.551GlnArg: 1.551 ± 0.474
2.548GlnSer: 2.548 ± 0.609
2.105GlnThr: 2.105 ± 0.437
3.767GlnVal: 3.767 ± 0.658
0.554GlnTrp: 0.554 ± 0.225
1.219GlnTyr: 1.219 ± 0.289
0.0GlnXaa: 0.0 ± 0.0
Arg
2.881ArgAla: 2.881 ± 0.578
0.332ArgCys: 0.332 ± 0.188
2.548ArgAsp: 2.548 ± 0.533
2.327ArgGlu: 2.327 ± 0.495
1.662ArgPhe: 1.662 ± 0.421
2.77ArgGly: 2.77 ± 0.525
0.222ArgHis: 0.222 ± 0.151
3.657ArgIle: 3.657 ± 0.619
3.102ArgLys: 3.102 ± 0.739
3.324ArgLeu: 3.324 ± 0.611
0.554ArgMet: 0.554 ± 0.232
2.327ArgAsn: 2.327 ± 0.51
1.108ArgPro: 1.108 ± 0.414
1.219ArgGln: 1.219 ± 0.409
2.438ArgArg: 2.438 ± 0.611
1.662ArgSer: 1.662 ± 0.351
2.881ArgThr: 2.881 ± 0.456
1.773ArgVal: 1.773 ± 0.403
0.332ArgTrp: 0.332 ± 0.176
2.216ArgTyr: 2.216 ± 0.474
0.0ArgXaa: 0.0 ± 0.0
Ser
2.992SerAla: 2.992 ± 0.606
0.222SerCys: 0.222 ± 0.146
4.543SerAsp: 4.543 ± 0.819
4.1SerGlu: 4.1 ± 0.72
2.992SerPhe: 2.992 ± 0.576
4.875SerGly: 4.875 ± 0.929
0.554SerHis: 0.554 ± 0.264
4.986SerIle: 4.986 ± 0.673
5.651SerLys: 5.651 ± 0.582
3.878SerLeu: 3.878 ± 0.402
0.886SerMet: 0.886 ± 0.356
4.543SerAsn: 4.543 ± 0.675
1.219SerPro: 1.219 ± 0.331
2.438SerGln: 2.438 ± 0.494
2.548SerArg: 2.548 ± 0.4
4.211SerSer: 4.211 ± 0.989
3.878SerThr: 3.878 ± 0.582
3.324SerVal: 3.324 ± 0.589
0.222SerTrp: 0.222 ± 0.151
2.659SerTyr: 2.659 ± 0.538
0.0SerXaa: 0.0 ± 0.0
Thr
5.54ThrAla: 5.54 ± 1.116
0.222ThrCys: 0.222 ± 0.184
3.324ThrAsp: 3.324 ± 0.774
4.1ThrGlu: 4.1 ± 0.564
2.77ThrPhe: 2.77 ± 0.488
5.097ThrGly: 5.097 ± 0.703
0.886ThrHis: 0.886 ± 0.276
4.543ThrIle: 4.543 ± 0.481
5.762ThrLys: 5.762 ± 0.725
5.429ThrLeu: 5.429 ± 0.774
0.997ThrMet: 0.997 ± 0.291
3.546ThrAsn: 3.546 ± 0.674
2.438ThrPro: 2.438 ± 0.775
2.992ThrGln: 2.992 ± 0.593
2.327ThrArg: 2.327 ± 0.478
3.767ThrSer: 3.767 ± 0.648
3.435ThrThr: 3.435 ± 0.597
4.765ThrVal: 4.765 ± 0.858
0.554ThrTrp: 0.554 ± 0.226
2.548ThrTyr: 2.548 ± 0.715
0.0ThrXaa: 0.0 ± 0.0
Val
4.321ValAla: 4.321 ± 0.864
0.554ValCys: 0.554 ± 0.262
3.546ValAsp: 3.546 ± 0.683
4.1ValGlu: 4.1 ± 0.572
1.884ValPhe: 1.884 ± 0.426
3.878ValGly: 3.878 ± 0.597
0.997ValHis: 0.997 ± 0.388
4.543ValIle: 4.543 ± 0.689
5.762ValLys: 5.762 ± 0.575
4.211ValLeu: 4.211 ± 0.741
1.219ValMet: 1.219 ± 0.419
2.438ValAsn: 2.438 ± 0.429
1.662ValPro: 1.662 ± 0.437
2.77ValGln: 2.77 ± 0.516
2.659ValArg: 2.659 ± 0.514
3.657ValSer: 3.657 ± 0.645
4.765ValThr: 4.765 ± 0.746
4.875ValVal: 4.875 ± 0.922
0.332ValTrp: 0.332 ± 0.177
1.773ValTyr: 1.773 ± 0.418
0.0ValXaa: 0.0 ± 0.0
Trp
0.554TrpAla: 0.554 ± 0.284
0.111TrpCys: 0.111 ± 0.134
0.886TrpAsp: 0.886 ± 0.355
0.554TrpGlu: 0.554 ± 0.392
0.443TrpPhe: 0.443 ± 0.198
0.665TrpGly: 0.665 ± 0.258
0.222TrpHis: 0.222 ± 0.135
0.554TrpIle: 0.554 ± 0.247
1.108TrpLys: 1.108 ± 0.295
1.662TrpLeu: 1.662 ± 0.462
0.222TrpMet: 0.222 ± 0.169
0.776TrpAsn: 0.776 ± 0.299
0.0TrpPro: 0.0 ± 0.0
0.665TrpGln: 0.665 ± 0.312
0.665TrpArg: 0.665 ± 0.27
0.332TrpSer: 0.332 ± 0.16
0.332TrpThr: 0.332 ± 0.166
0.665TrpVal: 0.665 ± 0.242
0.443TrpTrp: 0.443 ± 0.222
0.332TrpTyr: 0.332 ± 0.184
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.327TyrAla: 2.327 ± 0.441
0.222TyrCys: 0.222 ± 0.156
2.881TyrAsp: 2.881 ± 0.639
2.105TyrGlu: 2.105 ± 0.414
2.105TyrPhe: 2.105 ± 0.439
2.105TyrGly: 2.105 ± 0.472
0.332TyrHis: 0.332 ± 0.194
2.659TyrIle: 2.659 ± 0.675
2.881TyrLys: 2.881 ± 0.546
3.657TyrLeu: 3.657 ± 0.747
0.886TyrMet: 0.886 ± 0.295
1.662TyrAsn: 1.662 ± 0.553
1.33TyrPro: 1.33 ± 0.396
1.662TyrGln: 1.662 ± 0.434
1.108TyrArg: 1.108 ± 0.337
1.994TyrSer: 1.994 ± 0.391
2.105TyrThr: 2.105 ± 0.394
1.884TyrVal: 1.884 ± 0.559
0.665TyrTrp: 0.665 ± 0.31
1.44TyrTyr: 1.44 ± 0.364
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (9026 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski