Serratia sp. (strain ATCC 39006)
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4305 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I5TKY2|A0A2I5TKY2_SERS3 Uncharacterized protein OS=Serratia sp. (strain ATCC 39006) OX=104623 GN=CWC46_14325 PE=4 SV=1
MM1 pKa = 7.11 KK2 pKa = 9.92 TIDD5 pKa = 3.48 YY6 pKa = 10.07 HH7 pKa = 5.46 GTTICVLDD15 pKa = 4.95 DD16 pKa = 3.94 NDD18 pKa = 4.65 MLTDD22 pKa = 4.16 CPIGSYY28 pKa = 10.77 AVIEE32 pKa = 4.25 DD33 pKa = 3.48 SGFYY37 pKa = 10.03 VAVRR41 pKa = 11.84 VDD43 pKa = 3.68 EE44 pKa = 4.57 KK45 pKa = 10.98 DD46 pKa = 3.11 APTIHH51 pKa = 7.03 TDD53 pKa = 3.51 PVPTLEE59 pKa = 4.89 DD60 pKa = 3.54 ALDD63 pKa = 4.37 IIAEE67 pKa = 3.92 QHH69 pKa = 6.31 AFFSS73 pKa = 4.2
Molecular weight: 8.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.872
IPC_protein 3.834
Toseland 3.617
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.821
Rodwell 3.656
Grimsley 3.528
Solomon 3.821
Lehninger 3.783
Nozaki 3.973
DTASelect 4.228
Thurlkill 3.694
EMBOSS 3.821
Sillero 3.961
Patrickios 0.846
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.847
Protein with the highest isoelectric point:
>tr|A0A2I5THC1|A0A2I5THC1_SERS3 2-C-methyl-D-erythritol 2 4-cyclodiphosphate synthase OS=Serratia sp. (strain ATCC 39006) OX=104623 GN=ispF PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.36 GRR39 pKa = 11.84 TRR41 pKa = 11.84 LTVSKK46 pKa = 10.95
Molecular weight: 5.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4305
0
4305
1404364
19
5063
326.2
36.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.204 ± 0.043
1.155 ± 0.015
5.212 ± 0.03
5.311 ± 0.035
3.804 ± 0.033
7.257 ± 0.037
2.459 ± 0.02
6.25 ± 0.03
3.978 ± 0.034
11.017 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.721 ± 0.022
3.793 ± 0.027
4.462 ± 0.027
4.736 ± 0.039
5.602 ± 0.038
6.259 ± 0.039
5.482 ± 0.048
7.017 ± 0.028
1.395 ± 0.016
2.885 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here