Candidatus Endolissoclinum faulkneri L5
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 770 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V9TRK3|V9TRK3_9PROT PtzF OS=Candidatus Endolissoclinum faulkneri L5 OX=1401328 GN=ajuD PE=3 SV=1
MM1 pKa = 7.49 YY2 pKa = 8.29 FTDD5 pKa = 3.61 IQIVLYY11 pKa = 10.57 DD12 pKa = 3.82 LGEE15 pKa = 4.69 SIMEE19 pKa = 4.11 YY20 pKa = 10.9 FSMGGYY26 pKa = 9.27 AAYY29 pKa = 9.84 VWPSYY34 pKa = 10.28 GIAMLILVGLLINSWSITRR53 pKa = 11.84 RR54 pKa = 11.84 YY55 pKa = 9.12 EE56 pKa = 3.82 ADD58 pKa = 4.9 LICMCQNRR66 pKa = 11.84 SDD68 pKa = 4.14 IEE70 pKa = 4.14 PVSIDD75 pKa = 3.13 ITEE78 pKa = 4.01 KK79 pKa = 10.91
Molecular weight: 9.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.007
IPC2_protein 4.126
IPC_protein 3.961
Toseland 3.783
ProMoST 4.126
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.872
Rodwell 3.808
Grimsley 3.706
Solomon 3.923
Lehninger 3.872
Nozaki 4.075
DTASelect 4.24
Thurlkill 3.846
EMBOSS 3.884
Sillero 4.088
Patrickios 0.095
IPC_peptide 3.923
IPC2_peptide 4.062
IPC2.peptide.svr19 3.982
Protein with the highest isoelectric point:
>tr|V9TRG0|V9TRG0_9PROT Ferric uptake regulation protein OS=Candidatus Endolissoclinum faulkneri L5 OX=1401328 GN=np20 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.37 RR3 pKa = 11.84 TYY5 pKa = 10.19 QPSILVRR12 pKa = 11.84 KK13 pKa = 8.81 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.45 GFRR19 pKa = 11.84 LRR21 pKa = 11.84 MSTVGGRR28 pKa = 11.84 NVIRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.76 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
770
0
770
280757
44
6504
364.6
40.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.875 ± 0.123
1.251 ± 0.032
5.274 ± 0.06
5.357 ± 0.078
3.821 ± 0.056
6.968 ± 0.084
2.048 ± 0.035
8.318 ± 0.086
5.166 ± 0.097
10.12 ± 0.082
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.536 ± 0.053
4.659 ± 0.055
4.037 ± 0.051
3.02 ± 0.05
5.858 ± 0.07
6.776 ± 0.075
5.206 ± 0.04
6.946 ± 0.07
1.09 ± 0.037
2.676 ± 0.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here