Amino acid dipepetide frequency for Lactobacillus oryzae JCM 18671

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.723AlaAla: 6.723 ± 0.139
0.309AlaCys: 0.309 ± 0.022
5.05AlaAsp: 5.05 ± 0.11
5.081AlaGlu: 5.081 ± 0.131
3.331AlaPhe: 3.331 ± 0.09
5.686AlaGly: 5.686 ± 0.118
1.52AlaHis: 1.52 ± 0.052
6.013AlaIle: 6.013 ± 0.125
5.884AlaLys: 5.884 ± 0.102
7.647AlaLeu: 7.647 ± 0.131
2.3AlaMet: 2.3 ± 0.069
3.874AlaAsn: 3.874 ± 0.088
2.296AlaPro: 2.296 ± 0.071
3.33AlaGln: 3.33 ± 0.086
2.835AlaArg: 2.835 ± 0.07
4.55AlaSer: 4.55 ± 0.105
5.278AlaThr: 5.278 ± 0.106
6.021AlaVal: 6.021 ± 0.125
0.653AlaTrp: 0.653 ± 0.032
2.676AlaTyr: 2.676 ± 0.067
0.0AlaXaa: 0.0 ± 0.0
Cys
0.29CysAla: 0.29 ± 0.026
0.017CysCys: 0.017 ± 0.006
0.204CysAsp: 0.204 ± 0.02
0.198CysGlu: 0.198 ± 0.021
0.152CysPhe: 0.152 ± 0.016
0.372CysGly: 0.372 ± 0.025
0.112CysHis: 0.112 ± 0.014
0.217CysIle: 0.217 ± 0.02
0.124CysLys: 0.124 ± 0.017
0.423CysLeu: 0.423 ± 0.035
0.086CysMet: 0.086 ± 0.014
0.131CysAsn: 0.131 ± 0.015
0.172CysPro: 0.172 ± 0.023
0.15CysGln: 0.15 ± 0.016
0.182CysArg: 0.182 ± 0.019
0.182CysSer: 0.182 ± 0.017
0.172CysThr: 0.172 ± 0.018
0.249CysVal: 0.249 ± 0.021
0.051CysTrp: 0.051 ± 0.01
0.165CysTyr: 0.165 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
4.584AspAla: 4.584 ± 0.096
0.223AspCys: 0.223 ± 0.022
3.756AspAsp: 3.756 ± 0.101
4.514AspGlu: 4.514 ± 0.102
2.802AspPhe: 2.802 ± 0.072
3.912AspGly: 3.912 ± 0.083
1.413AspHis: 1.413 ± 0.06
3.736AspIle: 3.736 ± 0.092
3.562AspLys: 3.562 ± 0.097
5.605AspLeu: 5.605 ± 0.114
1.527AspMet: 1.527 ± 0.052
2.583AspAsn: 2.583 ± 0.075
2.205AspPro: 2.205 ± 0.062
3.021AspGln: 3.021 ± 0.08
2.497AspArg: 2.497 ± 0.068
3.174AspSer: 3.174 ± 0.09
3.058AspThr: 3.058 ± 0.082
4.179AspVal: 4.179 ± 0.091
0.765AspTrp: 0.765 ± 0.041
2.508AspTyr: 2.508 ± 0.077
0.0AspXaa: 0.0 ± 0.0
Glu
5.257GluAla: 5.257 ± 0.139
0.146GluCys: 0.146 ± 0.017
3.193GluAsp: 3.193 ± 0.087
3.749GluGlu: 3.749 ± 0.096
2.257GluPhe: 2.257 ± 0.066
3.137GluGly: 3.137 ± 0.083
1.224GluHis: 1.224 ± 0.053
4.249GluIle: 4.249 ± 0.105
4.305GluLys: 4.305 ± 0.103
6.208GluLeu: 6.208 ± 0.13
1.834GluMet: 1.834 ± 0.064
2.927GluAsn: 2.927 ± 0.089
1.943GluPro: 1.943 ± 0.069
2.955GluGln: 2.955 ± 0.087
2.862GluArg: 2.862 ± 0.089
3.343GluSer: 3.343 ± 0.088
3.938GluThr: 3.938 ± 0.091
4.215GluVal: 4.215 ± 0.11
0.605GluTrp: 0.605 ± 0.039
1.636GluTyr: 1.636 ± 0.058
0.0GluXaa: 0.0 ± 0.0
Phe
3.116PheAla: 3.116 ± 0.099
0.202PheCys: 0.202 ± 0.019
2.938PheAsp: 2.938 ± 0.074
2.573PheGlu: 2.573 ± 0.075
1.735PhePhe: 1.735 ± 0.074
3.202PheGly: 3.202 ± 0.083
0.803PheHis: 0.803 ± 0.043
2.766PheIle: 2.766 ± 0.078
2.594PheLys: 2.594 ± 0.066
3.607PheLeu: 3.607 ± 0.093
1.052PheMet: 1.052 ± 0.046
2.205PheAsn: 2.205 ± 0.06
1.456PhePro: 1.456 ± 0.052
1.509PheGln: 1.509 ± 0.049
1.392PheArg: 1.392 ± 0.047
2.873PheSer: 2.873 ± 0.09
2.544PheThr: 2.544 ± 0.074
3.2PheVal: 3.2 ± 0.084
0.464PheTrp: 0.464 ± 0.031
1.561PheTyr: 1.561 ± 0.054
0.0PheXaa: 0.0 ± 0.0
Gly
5.005GlyAla: 5.005 ± 0.112
0.299GlyCys: 0.299 ± 0.023
3.629GlyAsp: 3.629 ± 0.084
3.728GlyGlu: 3.728 ± 0.088
3.081GlyPhe: 3.081 ± 0.078
4.408GlyGly: 4.408 ± 0.142
1.518GlyHis: 1.518 ± 0.056
5.388GlyIle: 5.388 ± 0.114
4.554GlyLys: 4.554 ± 0.096
6.444GlyLeu: 6.444 ± 0.128
2.006GlyMet: 2.006 ± 0.063
2.819GlyAsn: 2.819 ± 0.077
1.653GlyPro: 1.653 ± 0.06
2.935GlyGln: 2.935 ± 0.086
2.742GlyArg: 2.742 ± 0.071
4.046GlySer: 4.046 ± 0.087
4.49GlyThr: 4.49 ± 0.087
4.977GlyVal: 4.977 ± 0.095
0.73GlyTrp: 0.73 ± 0.041
2.588GlyTyr: 2.588 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
1.379HisAla: 1.379 ± 0.058
0.095HisCys: 0.095 ± 0.015
1.295HisAsp: 1.295 ± 0.045
1.252HisGlu: 1.252 ± 0.055
1.099HisPhe: 1.099 ± 0.048
1.516HisGly: 1.516 ± 0.053
0.651HisHis: 0.651 ± 0.041
1.187HisIle: 1.187 ± 0.05
1.031HisLys: 1.031 ± 0.044
2.057HisLeu: 2.057 ± 0.066
0.517HisMet: 0.517 ± 0.033
0.863HisAsn: 0.863 ± 0.041
1.005HisPro: 1.005 ± 0.046
1.061HisGln: 1.061 ± 0.049
0.936HisArg: 0.936 ± 0.046
1.114HisSer: 1.114 ± 0.051
1.033HisThr: 1.033 ± 0.04
1.523HisVal: 1.523 ± 0.06
0.253HisTrp: 0.253 ± 0.02
0.853HisTyr: 0.853 ± 0.044
0.0HisXaa: 0.0 ± 0.0
Ile
5.772IleAla: 5.772 ± 0.12
0.359IleCys: 0.359 ± 0.03
4.389IleAsp: 4.389 ± 0.099
4.204IleGlu: 4.204 ± 0.099
2.762IlePhe: 2.762 ± 0.082
4.999IleGly: 4.999 ± 0.109
1.275IleHis: 1.275 ± 0.049
4.752IleIle: 4.752 ± 0.128
4.155IleLys: 4.155 ± 0.088
6.264IleLeu: 6.264 ± 0.13
1.752IleMet: 1.752 ± 0.066
3.335IleAsn: 3.335 ± 0.095
2.639IlePro: 2.639 ± 0.081
2.796IleGln: 2.796 ± 0.076
2.736IleArg: 2.736 ± 0.075
4.67IleSer: 4.67 ± 0.105
4.243IleThr: 4.243 ± 0.093
5.139IleVal: 5.139 ± 0.108
0.586IleTrp: 0.586 ± 0.035
2.018IleTyr: 2.018 ± 0.063
0.0IleXaa: 0.0 ± 0.0
Lys
5.149LysAla: 5.149 ± 0.109
0.112LysCys: 0.112 ± 0.014
3.448LysAsp: 3.448 ± 0.085
3.597LysGlu: 3.597 ± 0.095
2.076LysPhe: 2.076 ± 0.065
3.476LysGly: 3.476 ± 0.076
1.222LysHis: 1.222 ± 0.053
4.02LysIle: 4.02 ± 0.083
4.565LysLys: 4.565 ± 0.122
6.257LysLeu: 6.257 ± 0.118
2.255LysMet: 2.255 ± 0.06
3.193LysAsn: 3.193 ± 0.085
2.414LysPro: 2.414 ± 0.065
3.558LysGln: 3.558 ± 0.091
3.316LysArg: 3.316 ± 0.087
3.721LysSer: 3.721 ± 0.098
4.269LysThr: 4.269 ± 0.099
4.473LysVal: 4.473 ± 0.103
0.634LysTrp: 0.634 ± 0.031
2.386LysTyr: 2.386 ± 0.08
0.0LysXaa: 0.0 ± 0.0
Leu
8.787LeuAla: 8.787 ± 0.138
0.359LeuCys: 0.359 ± 0.027
5.707LeuAsp: 5.707 ± 0.124
5.008LeuGlu: 5.008 ± 0.113
3.895LeuPhe: 3.895 ± 0.101
6.337LeuGly: 6.337 ± 0.125
1.602LeuHis: 1.602 ± 0.052
6.803LeuIle: 6.803 ± 0.137
6.433LeuLys: 6.433 ± 0.11
8.63LeuLeu: 8.63 ± 0.173
2.675LeuMet: 2.675 ± 0.07
4.898LeuAsn: 4.898 ± 0.099
3.947LeuPro: 3.947 ± 0.092
3.68LeuGln: 3.68 ± 0.095
3.715LeuArg: 3.715 ± 0.084
6.62LeuSer: 6.62 ± 0.106
6.669LeuThr: 6.669 ± 0.128
6.775LeuVal: 6.775 ± 0.12
0.777LeuTrp: 0.777 ± 0.04
2.617LeuTyr: 2.617 ± 0.087
0.0LeuXaa: 0.0 ± 0.0
Met
2.583MetAla: 2.583 ± 0.064
0.097MetCys: 0.097 ± 0.014
1.477MetAsp: 1.477 ± 0.05
1.187MetGlu: 1.187 ± 0.049
0.857MetPhe: 0.857 ± 0.045
1.875MetGly: 1.875 ± 0.067
0.481MetHis: 0.481 ± 0.03
1.9MetIle: 1.9 ± 0.06
2.048MetLys: 2.048 ± 0.065
2.197MetLeu: 2.197 ± 0.07
0.872MetMet: 0.872 ± 0.044
1.394MetAsn: 1.394 ± 0.052
1.119MetPro: 1.119 ± 0.043
1.086MetGln: 1.086 ± 0.044
1.104MetArg: 1.104 ± 0.043
1.782MetSer: 1.782 ± 0.051
2.18MetThr: 2.18 ± 0.061
1.911MetVal: 1.911 ± 0.055
0.165MetTrp: 0.165 ± 0.017
0.599MetTyr: 0.599 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
3.633AsnAla: 3.633 ± 0.082
0.193AsnCys: 0.193 ± 0.018
2.892AsnAsp: 2.892 ± 0.072
3.126AsnGlu: 3.126 ± 0.08
1.894AsnPhe: 1.894 ± 0.057
3.616AsnGly: 3.616 ± 0.084
1.187AsnHis: 1.187 ± 0.052
2.721AsnIle: 2.721 ± 0.082
2.691AsnLys: 2.691 ± 0.083
4.353AsnLeu: 4.353 ± 0.087
1.183AsnMet: 1.183 ± 0.043
2.268AsnAsn: 2.268 ± 0.085
2.197AsnPro: 2.197 ± 0.066
2.832AsnGln: 2.832 ± 0.086
2.34AsnArg: 2.34 ± 0.071
2.542AsnSer: 2.542 ± 0.073
2.383AsnThr: 2.383 ± 0.07
3.111AsnVal: 3.111 ± 0.07
0.689AsnTrp: 0.689 ± 0.036
1.699AsnTyr: 1.699 ± 0.064
0.0AsnXaa: 0.0 ± 0.0
Pro
2.819ProAla: 2.819 ± 0.078
0.099ProCys: 0.099 ± 0.013
2.444ProAsp: 2.444 ± 0.077
3.004ProGlu: 3.004 ± 0.095
1.675ProPhe: 1.675 ± 0.059
2.313ProGly: 2.313 ± 0.064
0.631ProHis: 0.631 ± 0.034
2.525ProIle: 2.525 ± 0.066
2.311ProLys: 2.311 ± 0.064
3.318ProLeu: 3.318 ± 0.076
0.835ProMet: 0.835 ± 0.04
1.791ProAsn: 1.791 ± 0.056
0.545ProPro: 0.545 ± 0.027
1.321ProGln: 1.321 ± 0.048
1.151ProArg: 1.151 ± 0.048
1.997ProSer: 1.997 ± 0.056
2.377ProThr: 2.377 ± 0.068
2.918ProVal: 2.918 ± 0.066
0.288ProTrp: 0.288 ± 0.023
1.346ProTyr: 1.346 ± 0.048
0.0ProXaa: 0.0 ± 0.0
Gln
3.797GlnAla: 3.797 ± 0.094
0.095GlnCys: 0.095 ± 0.016
2.117GlnAsp: 2.117 ± 0.055
2.401GlnGlu: 2.401 ± 0.078
2.003GlnPhe: 2.003 ± 0.067
2.184GlnGly: 2.184 ± 0.06
0.893GlnHis: 0.893 ± 0.042
3.234GlnIle: 3.234 ± 0.077
2.815GlnLys: 2.815 ± 0.069
5.248GlnLeu: 5.248 ± 0.117
1.19GlnMet: 1.19 ± 0.047
2.229GlnAsn: 2.229 ± 0.069
1.626GlnPro: 1.626 ± 0.054
2.471GlnGln: 2.471 ± 0.098
2.083GlnArg: 2.083 ± 0.067
2.523GlnSer: 2.523 ± 0.071
2.957GlnThr: 2.957 ± 0.067
3.337GlnVal: 3.337 ± 0.077
0.427GlnTrp: 0.427 ± 0.028
1.467GlnTyr: 1.467 ± 0.055
0.0GlnXaa: 0.0 ± 0.0
Arg
2.856ArgAla: 2.856 ± 0.076
0.137ArgCys: 0.137 ± 0.014
2.403ArgAsp: 2.403 ± 0.074
2.74ArgGlu: 2.74 ± 0.079
2.048ArgPhe: 2.048 ± 0.064
2.51ArgGly: 2.51 ± 0.067
1.007ArgHis: 1.007 ± 0.041
2.804ArgIle: 2.804 ± 0.088
2.673ArgLys: 2.673 ± 0.075
4.393ArgLeu: 4.393 ± 0.098
1.072ArgMet: 1.072 ± 0.044
1.943ArgAsn: 1.943 ± 0.065
1.449ArgPro: 1.449 ± 0.056
2.295ArgGln: 2.295 ± 0.072
2.1ArgArg: 2.1 ± 0.067
2.156ArgSer: 2.156 ± 0.058
2.179ArgThr: 2.179 ± 0.06
2.839ArgVal: 2.839 ± 0.072
0.378ArgTrp: 0.378 ± 0.026
1.645ArgTyr: 1.645 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
4.614SerAla: 4.614 ± 0.1
0.185SerCys: 0.185 ± 0.021
3.702SerAsp: 3.702 ± 0.085
3.738SerGlu: 3.738 ± 0.088
2.547SerPhe: 2.547 ± 0.072
4.687SerGly: 4.687 ± 0.112
1.336SerHis: 1.336 ± 0.054
3.947SerIle: 3.947 ± 0.089
3.749SerLys: 3.749 ± 0.107
5.828SerLeu: 5.828 ± 0.109
1.516SerMet: 1.516 ± 0.05
2.796SerAsn: 2.796 ± 0.086
1.902SerPro: 1.902 ± 0.06
2.633SerGln: 2.633 ± 0.073
2.547SerArg: 2.547 ± 0.08
3.859SerSer: 3.859 ± 0.126
3.696SerThr: 3.696 ± 0.107
4.267SerVal: 4.267 ± 0.097
0.633SerTrp: 0.633 ± 0.038
2.175SerTyr: 2.175 ± 0.064
0.0SerXaa: 0.0 ± 0.0
Thr
5.177ThrAla: 5.177 ± 0.108
0.213ThrCys: 0.213 ± 0.024
3.93ThrAsp: 3.93 ± 0.107
3.579ThrGlu: 3.579 ± 0.104
2.6ThrPhe: 2.6 ± 0.071
4.75ThrGly: 4.75 ± 0.09
1.314ThrHis: 1.314 ± 0.049
4.694ThrIle: 4.694 ± 0.091
3.964ThrLys: 3.964 ± 0.093
5.879ThrLeu: 5.879 ± 0.127
1.407ThrMet: 1.407 ± 0.047
2.895ThrAsn: 2.895 ± 0.081
2.729ThrPro: 2.729 ± 0.065
2.403ThrGln: 2.403 ± 0.075
2.094ThrArg: 2.094 ± 0.062
3.704ThrSer: 3.704 ± 0.083
4.084ThrThr: 4.084 ± 0.099
4.977ThrVal: 4.977 ± 0.097
0.599ThrTrp: 0.599 ± 0.037
2.257ThrTyr: 2.257 ± 0.073
0.0ThrXaa: 0.0 ± 0.0
Val
6.519ValAla: 6.519 ± 0.128
0.324ValCys: 0.324 ± 0.025
4.406ValAsp: 4.406 ± 0.104
4.005ValGlu: 4.005 ± 0.108
2.787ValPhe: 2.787 ± 0.08
5.093ValGly: 5.093 ± 0.092
1.387ValHis: 1.387 ± 0.053
5.257ValIle: 5.257 ± 0.12
4.638ValLys: 4.638 ± 0.092
6.541ValLeu: 6.541 ± 0.116
1.855ValMet: 1.855 ± 0.061
3.474ValAsn: 3.474 ± 0.085
2.811ValPro: 2.811 ± 0.079
2.506ValGln: 2.506 ± 0.063
2.673ValArg: 2.673 ± 0.079
4.847ValSer: 4.847 ± 0.104
5.139ValThr: 5.139 ± 0.103
5.576ValVal: 5.576 ± 0.115
0.636ValTrp: 0.636 ± 0.036
2.222ValTyr: 2.222 ± 0.063
0.0ValXaa: 0.0 ± 0.0
Trp
0.644TrpAla: 0.644 ± 0.034
0.043TrpCys: 0.043 ± 0.009
0.479TrpAsp: 0.479 ± 0.034
0.444TrpGlu: 0.444 ± 0.028
0.511TrpPhe: 0.511 ± 0.032
0.605TrpGly: 0.605 ± 0.033
0.296TrpHis: 0.296 ± 0.023
0.683TrpIle: 0.683 ± 0.035
0.421TrpLys: 0.421 ± 0.033
1.316TrpLeu: 1.316 ± 0.062
0.251TrpMet: 0.251 ± 0.021
0.464TrpAsn: 0.464 ± 0.032
0.288TrpPro: 0.288 ± 0.022
0.649TrpGln: 0.649 ± 0.034
0.507TrpArg: 0.507 ± 0.031
0.586TrpSer: 0.586 ± 0.038
0.543TrpThr: 0.543 ± 0.029
0.648TrpVal: 0.648 ± 0.036
0.163TrpTrp: 0.163 ± 0.017
0.376TrpTyr: 0.376 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.356TyrAla: 2.356 ± 0.059
0.138TyrCys: 0.138 ± 0.018
2.165TyrAsp: 2.165 ± 0.061
1.853TyrGlu: 1.853 ± 0.068
1.683TyrPhe: 1.683 ± 0.056
2.362TyrGly: 2.362 ± 0.069
0.85TyrHis: 0.85 ± 0.042
1.948TyrIle: 1.948 ± 0.064
1.626TyrLys: 1.626 ± 0.063
3.719TyrLeu: 3.719 ± 0.097
0.726TyrMet: 0.726 ± 0.036
1.465TyrAsn: 1.465 ± 0.054
1.312TyrPro: 1.312 ± 0.051
1.909TyrGln: 1.909 ± 0.06
1.812TyrArg: 1.812 ± 0.061
2.07TyrSer: 2.07 ± 0.053
2.018TyrThr: 2.018 ± 0.062
2.36TyrVal: 2.36 ± 0.064
0.421TyrTrp: 0.421 ± 0.032
1.308TyrTyr: 1.308 ± 0.059
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1890 proteins (534300 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski