Pandoraea thiooxydans
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3963 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0G3ELZ5|A0A0G3ELZ5_9BURK 4-hydroxyphenylacetate isomerase OS=Pandoraea thiooxydans OX=445709 GN=ABW99_06770 PE=4 SV=1
MM1 pKa = 7.86 PEE3 pKa = 3.67 ATEE6 pKa = 4.19 CLVIASQPLDD16 pKa = 3.62 CLRR19 pKa = 11.84 VSLADD24 pKa = 3.64 AACVLIPAFSDD35 pKa = 3.93 GPAAVGLSSRR45 pKa = 11.84 GLPLSGDD52 pKa = 3.7 IAWAALCVGG61 pKa = 4.32
Molecular weight: 6.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.821
IPC2_protein 3.986
IPC_protein 3.757
Toseland 3.579
ProMoST 3.986
Dawson 3.783
Bjellqvist 3.961
Wikipedia 3.783
Rodwell 3.617
Grimsley 3.516
Solomon 3.732
Lehninger 3.694
Nozaki 3.948
DTASelect 4.126
Thurlkill 3.694
EMBOSS 3.783
Sillero 3.897
Patrickios 0.477
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.823
Protein with the highest isoelectric point:
>tr|A0A0G3ENV5|A0A0G3ENV5_9BURK Nickel transporter OS=Pandoraea thiooxydans OX=445709 GN=ABW99_05110 PE=3 SV=1
MM1 pKa = 6.99 TKK3 pKa = 10.55 KK4 pKa = 10.41 NAFTSAARR12 pKa = 11.84 LLAAFSIVLSSFVGCAATPISPHH35 pKa = 6.55 ADD37 pKa = 2.97 RR38 pKa = 11.84 AVGQPGTGIAGLTGRR53 pKa = 11.84 WVLLSWVGQHH63 pKa = 7.04 ASPPPAARR71 pKa = 11.84 NVTLVLSKK79 pKa = 10.91 DD80 pKa = 3.31 GAYY83 pKa = 10.29 GGSGGCNRR91 pKa = 11.84 IFGQYY96 pKa = 9.59 HH97 pKa = 6.2 VGPAHH102 pKa = 5.53 QQISVNPPATTRR114 pKa = 11.84 MSCPVDD120 pKa = 3.46 VMAFEE125 pKa = 4.65 SRR127 pKa = 11.84 FLATLAHH134 pKa = 5.78 VNRR137 pKa = 11.84 FEE139 pKa = 4.26 LTGNTLVLFVRR150 pKa = 11.84 DD151 pKa = 3.56 TDD153 pKa = 3.45 RR154 pKa = 11.84 LTFARR159 pKa = 11.84 QVIPRR164 pKa = 11.84 QGSLADD170 pKa = 3.7 NAPGKK175 pKa = 10.96 SMASGRR181 pKa = 11.84 TPAA184 pKa = 4.28
Molecular weight: 19.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.56
IPC_protein 10.496
Toseland 10.701
ProMoST 10.467
Dawson 10.774
Bjellqvist 10.54
Wikipedia 11.023
Rodwell 10.877
Grimsley 10.818
Solomon 10.95
Lehninger 10.906
Nozaki 10.716
DTASelect 10.526
Thurlkill 10.701
EMBOSS 11.111
Sillero 10.73
Patrickios 10.657
IPC_peptide 10.95
IPC2_peptide 9.853
IPC2.peptide.svr19 8.608
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3963
0
3963
1287374
27
3911
324.8
35.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.758 ± 0.05
0.987 ± 0.011
5.15 ± 0.033
5.026 ± 0.04
3.653 ± 0.024
8.264 ± 0.041
2.44 ± 0.016
4.858 ± 0.025
2.936 ± 0.031
10.895 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.442 ± 0.016
2.643 ± 0.027
5.176 ± 0.025
3.953 ± 0.025
7.036 ± 0.038
5.285 ± 0.029
5.134 ± 0.035
7.499 ± 0.033
1.392 ± 0.018
2.473 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here