Phoenicopterus ruber ruber
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8220 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A091TU11|A0A091TU11_PHORB Mitochondrial glutamate carrier 1 (Fragment) OS=Phoenicopterus ruber ruber OX=9218 GN=N337_04182 PE=3 SV=1
DDD2 pKa = 4.19 QSLPGLANVDDD13 pKa = 4.79 SAQNPNIQVTIEEE26 pKa = 4.15 VDDD29 pKa = 4.31 DD30 pKa = 4.17 QAEEE34 pKa = 4.2 EEE36 pKa = 4.1 DDD38 pKa = 3.99 LKKK41 pKa = 10.12 EE42 pKa = 4.19 SNDDD46 pKa = 2.88 SLTSSEEE53 pKa = 4.14 LSHHH57 pKa = 7.2 KK58 pKa = 10.8 DD59 pKa = 3.65 FWPLFWEEE67 pKa = 4.38 YY68 pKa = 10.58 DDD70 pKa = 3.68 AEEE73 pKa = 4.28 EE74 pKa = 4.14 EE75 pKa = 4.18 EE76 pKa = 4.36 EE77 pKa = 4.18 EE78 pKa = 4.26 EE79 pKa = 4.41 EE80 pKa = 5.32 EE81 pKa = 5.2 EE82 pKa = 5.12 EE83 pKa = 4.63 EE84 pKa = 5.57 DD85 pKa = 5.08 DD86 pKa = 4.82 LDDD89 pKa = 3.38 GDDD92 pKa = 3.97 DDD94 pKa = 3.67 EE95 pKa = 4.78 EE96 pKa = 4.27 EE97 pKa = 4.23 EE98 pKa = 4.15 EE99 pKa = 4.17 EE100 pKa = 4.26 EE101 pKa = 4.18 EE102 pKa = 4.21 EE103 pKa = 4.21 EE104 pKa = 4.21 EE105 pKa = 4.21 EE106 pKa = 4.21 EE107 pKa = 4.21 EE108 pKa = 4.21 EE109 pKa = 4.32 EE110 pKa = 4.28 EE111 pKa = 4.87 EE112 pKa = 4.62 EE113 pKa = 4.43 EE114 pKa = 3.89 EE115 pKa = 5.43 DD116 pKa = 3.53 YY117 pKa = 10.46 AEEE120 pKa = 4.11 YY121 pKa = 10.59 EE122 pKa = 4.36 EE123 pKa = 4.71 EE124 pKa = 4.02 EE125 pKa = 4.51 MLSGVGGDDD134 pKa = 3.46 DDD136 pKa = 4.48 RR137 pKa = 11.84 WPGQKKK143 pKa = 9.55 WIFKKK148 pKa = 10.0 EE149 pKa = 4.0 KK150 pKa = 10.83 YY151 pKa = 8.82 YYY153 pKa = 10.35 DD154 pKa = 3.76 YY155 pKa = 11.17 EE156 pKa = 5.81 DD157 pKa = 3.87 EE158 pKa = 4.87 EE159 pKa = 4.71 EE160 pKa = 4.04 SPWSPCSITCGSGNQKKK177 pKa = 8.36 TRR179 pKa = 11.84 SCGYYY184 pKa = 9.46 CTATEEE190 pKa = 5.26 RR191 pKa = 11.84 TCDDD195 pKa = 3.49 PHHH198 pKa = 7.0 PGAEEE203 pKa = 3.73 EEE205 pKa = 4.48 VFPTEEE211 pKa = 3.84 EE212 pKa = 4.21 PFKKK216 pKa = 10.91 DDD218 pKa = 2.8 TTEEE222 pKa = 4.22 FNSEEE227 pKa = 3.77 DDD229 pKa = 4.04 CEEE232 pKa = 3.23 KK233 pKa = 9.96 LNCKKK238 pKa = 10.19 DDD240 pKa = 3.71 LTKKK244 pKa = 10.51 YY245 pKa = 9.97 SKKK248 pKa = 11.0 LTDDD252 pKa = 5.3 PSCPCSYYY260 pKa = 10.29 LEEE263 pKa = 4.37 VYYY266 pKa = 10.72 AVNLQDDD273 pKa = 3.34 EE274 pKa = 4.8 QGK
Molecular weight: 32.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.757
IPC_protein 3.732
Toseland 3.541
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.541
Rodwell 3.554
Grimsley 3.452
Solomon 3.668
Lehninger 3.617
Nozaki 3.783
DTASelect 3.91
Thurlkill 3.567
EMBOSS 3.554
Sillero 3.834
Patrickios 1.278
IPC_peptide 3.668
IPC2_peptide 3.821
IPC2.peptide.svr19 3.739
Protein with the highest isoelectric point:
>tr|A0A091UEQ4|A0A091UEQ4_PHORB Mitogen-activated protein kinase kinase kinase 3 (Fragment) OS=Phoenicopterus ruber ruber OX=9218 GN=N337_01822 PE=4 SV=1
TT1 pKa = 6.27 QSMRR5 pKa = 11.84 VTQSMKK11 pKa = 8.34 VTQSMRR17 pKa = 11.84 VTQSMKK23 pKa = 8.34 VTQSMRR29 pKa = 11.84 VTQSMKK35 pKa = 8.34 VTQSMRR41 pKa = 11.84 VTQSMKK47 pKa = 8.34 VTQSMRR53 pKa = 11.84 VTQSMKK59 pKa = 8.34 VTQSMRR65 pKa = 11.84 VTQSMKK71 pKa = 8.34 VTQSMRR77 pKa = 11.84 VTQSMKK83 pKa = 8.34 VTQSMRR89 pKa = 11.84 VTT91 pKa = 3.57
Molecular weight: 10.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.111
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.647
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.369
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.095
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8220
0
8220
3367651
31
4570
409.7
46.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.36 ± 0.023
2.242 ± 0.022
5.051 ± 0.019
7.226 ± 0.036
3.91 ± 0.02
5.774 ± 0.033
2.519 ± 0.012
5.038 ± 0.02
6.511 ± 0.036
9.698 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.246 ± 0.011
4.199 ± 0.018
5.229 ± 0.031
4.672 ± 0.026
5.191 ± 0.022
8.257 ± 0.04
5.413 ± 0.02
6.295 ± 0.022
1.177 ± 0.01
2.99 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here