Micromonospora sp. CB01531

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora; unclassified Micromonospora

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7693 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q4ZD15|A0A1Q4ZD15_9ACTN Glyoxalase OS=Micromonospora sp. CB01531 OX=1718947 GN=A6A27_38400 PE=4 SV=1
MM1 pKa = 8.31DD2 pKa = 4.22SQQSLHH8 pKa = 6.5VNSQQSVDD16 pKa = 3.6SSQTLHH22 pKa = 7.38DD23 pKa = 4.07FVLNLLTDD31 pKa = 4.23PDD33 pKa = 3.68ARR35 pKa = 11.84SAFDD39 pKa = 4.84LDD41 pKa = 4.23PEE43 pKa = 4.73GALQAAGLTDD53 pKa = 3.29VTAADD58 pKa = 3.9VQDD61 pKa = 3.98VVPLVVDD68 pKa = 3.91YY69 pKa = 10.3AAPGAGLAPLAPVAGQLGLDD89 pKa = 4.2PLASDD94 pKa = 3.45ATDD97 pKa = 3.36VVGQLAAAPQQISVNSTYY115 pKa = 11.27AGVDD119 pKa = 3.35VSAGALGAIAVDD131 pKa = 3.7PAGLYY136 pKa = 10.5AGAAVLPGIGLGIGPGGLATDD157 pKa = 4.05LTGAHH162 pKa = 6.64DD163 pKa = 3.86VAHH166 pKa = 6.39TLDD169 pKa = 3.83SDD171 pKa = 4.12VVGAVNTAVDD181 pKa = 4.03PVVGDD186 pKa = 3.82LAGTAGDD193 pKa = 4.14PLGTATGTGVLSGDD207 pKa = 3.62GVLGGDD213 pKa = 4.08LGVLSGTQAQVNGVVGSLGVDD234 pKa = 3.18HH235 pKa = 6.96TLGGLGLGDD244 pKa = 3.46GAGGVVPPVHH254 pKa = 6.69VPTTVDD260 pKa = 3.47GVTQQVNGTLSGVTGTVNGVSGTVGGLTAGVTGDD294 pKa = 3.67AGAHH298 pKa = 6.0GSATVHH304 pKa = 6.3GDD306 pKa = 2.84VHH308 pKa = 7.06ASSDD312 pKa = 3.48GGLLGGLLL320 pKa = 3.81

Molecular weight: 30.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q5AFR1|A0A1Q5AFR1_9ACTN Uncharacterized protein OS=Micromonospora sp. CB01531 OX=1718947 GN=A6A27_06445 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.93RR4 pKa = 11.84TYY6 pKa = 10.0QPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIISTRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.47GRR40 pKa = 11.84ARR42 pKa = 11.84LAAA45 pKa = 4.44

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7693

0

7693

2502689

29

11474

325.3

34.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.985 ± 0.05

0.741 ± 0.008

6.049 ± 0.023

5.017 ± 0.027

2.681 ± 0.017

9.251 ± 0.026

2.157 ± 0.015

3.263 ± 0.02

1.779 ± 0.024

10.558 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.619 ± 0.01

1.884 ± 0.019

6.359 ± 0.025

2.864 ± 0.018

8.342 ± 0.032

4.674 ± 0.027

6.113 ± 0.032

8.877 ± 0.028

1.647 ± 0.013

2.139 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski