Amino acid dipepetide frequency for Micromonospora sp. CB01531

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.143AlaAla: 23.143 ± 0.174
0.984AlaCys: 0.984 ± 0.022
8.861AlaAsp: 8.861 ± 0.067
8.362AlaGlu: 8.362 ± 0.072
3.286AlaPhe: 3.286 ± 0.035
14.348AlaGly: 14.348 ± 0.102
2.548AlaHis: 2.548 ± 0.036
4.082AlaIle: 4.082 ± 0.043
2.477AlaLys: 2.477 ± 0.041
14.418AlaLeu: 14.418 ± 0.113
2.455AlaMet: 2.455 ± 0.035
2.322AlaAsn: 2.322 ± 0.037
7.204AlaPro: 7.204 ± 0.069
3.702AlaGln: 3.702 ± 0.041
10.378AlaArg: 10.378 ± 0.082
5.57AlaSer: 5.57 ± 0.052
8.03AlaThr: 8.03 ± 0.071
12.696AlaVal: 12.696 ± 0.098
2.051AlaTrp: 2.051 ± 0.031
2.933AlaTyr: 2.933 ± 0.032
0.0AlaXaa: 0.0 ± 0.0
Cys
0.94CysAla: 0.94 ± 0.018
0.108CysCys: 0.108 ± 0.007
0.492CysAsp: 0.492 ± 0.014
0.306CysGlu: 0.306 ± 0.01
0.199CysPhe: 0.199 ± 0.009
0.903CysGly: 0.903 ± 0.02
0.193CysHis: 0.193 ± 0.009
0.139CysIle: 0.139 ± 0.007
0.098CysLys: 0.098 ± 0.007
0.698CysLeu: 0.698 ± 0.016
0.103CysMet: 0.103 ± 0.006
0.132CysAsn: 0.132 ± 0.007
0.503CysPro: 0.503 ± 0.016
0.207CysGln: 0.207 ± 0.01
0.66CysArg: 0.66 ± 0.02
0.381CysSer: 0.381 ± 0.014
0.437CysThr: 0.437 ± 0.016
0.595CysVal: 0.595 ± 0.015
0.14CysTrp: 0.14 ± 0.006
0.178CysTyr: 0.178 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.554AspAla: 7.554 ± 0.057
0.388AspCys: 0.388 ± 0.013
3.641AspAsp: 3.641 ± 0.047
3.673AspGlu: 3.673 ± 0.046
1.526AspPhe: 1.526 ± 0.026
6.406AspGly: 6.406 ± 0.06
1.316AspHis: 1.316 ± 0.023
1.715AspIle: 1.715 ± 0.027
0.991AspLys: 0.991 ± 0.023
6.769AspLeu: 6.769 ± 0.067
0.734AspMet: 0.734 ± 0.017
1.04AspAsn: 1.04 ± 0.024
4.925AspPro: 4.925 ± 0.048
1.817AspGln: 1.817 ± 0.03
5.62AspArg: 5.62 ± 0.055
2.255AspSer: 2.255 ± 0.035
2.964AspThr: 2.964 ± 0.04
4.952AspVal: 4.952 ± 0.046
1.055AspTrp: 1.055 ± 0.022
1.15AspTyr: 1.15 ± 0.024
0.0AspXaa: 0.0 ± 0.0
Glu
6.453GluAla: 6.453 ± 0.061
0.336GluCys: 0.336 ± 0.012
2.086GluAsp: 2.086 ± 0.034
2.542GluGlu: 2.542 ± 0.04
1.418GluPhe: 1.418 ± 0.026
3.184GluGly: 3.184 ± 0.034
1.335GluHis: 1.335 ± 0.023
2.089GluIle: 2.089 ± 0.031
1.003GluLys: 1.003 ± 0.024
6.561GluLeu: 6.561 ± 0.064
0.792GluMet: 0.792 ± 0.017
0.845GluAsn: 0.845 ± 0.02
3.351GluPro: 3.351 ± 0.042
2.198GluGln: 2.198 ± 0.033
4.942GluArg: 4.942 ± 0.053
2.154GluSer: 2.154 ± 0.035
2.486GluThr: 2.486 ± 0.033
4.588GluVal: 4.588 ± 0.045
0.732GluTrp: 0.732 ± 0.017
1.071GluTyr: 1.071 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
3.728PheAla: 3.728 ± 0.04
0.233PheCys: 0.233 ± 0.01
1.992PheAsp: 1.992 ± 0.032
1.204PheGlu: 1.204 ± 0.025
0.842PhePhe: 0.842 ± 0.023
2.933PheGly: 2.933 ± 0.036
0.581PheHis: 0.581 ± 0.016
0.748PheIle: 0.748 ± 0.018
0.424PheLys: 0.424 ± 0.013
2.562PheLeu: 2.562 ± 0.04
0.33PheMet: 0.33 ± 0.012
0.614PheAsn: 0.614 ± 0.02
1.396PhePro: 1.396 ± 0.027
0.667PheGln: 0.667 ± 0.016
1.808PheArg: 1.808 ± 0.032
1.347PheSer: 1.347 ± 0.024
1.98PheThr: 1.98 ± 0.031
2.359PheVal: 2.359 ± 0.033
0.454PheTrp: 0.454 ± 0.013
0.611PheTyr: 0.611 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
10.719GlyAla: 10.719 ± 0.087
0.83GlyCys: 0.83 ± 0.02
5.24GlyAsp: 5.24 ± 0.055
4.66GlyGlu: 4.66 ± 0.045
2.798GlyPhe: 2.798 ± 0.038
8.574GlyGly: 8.574 ± 0.084
2.14GlyHis: 2.14 ± 0.03
3.21GlyIle: 3.21 ± 0.041
2.073GlyLys: 2.073 ± 0.032
9.439GlyLeu: 9.439 ± 0.074
1.863GlyMet: 1.863 ± 0.03
1.876GlyAsn: 1.876 ± 0.036
5.236GlyPro: 5.236 ± 0.053
3.006GlyGln: 3.006 ± 0.04
7.965GlyArg: 7.965 ± 0.061
4.759GlySer: 4.759 ± 0.054
5.737GlyThr: 5.737 ± 0.061
7.967GlyVal: 7.967 ± 0.059
1.988GlyTrp: 1.988 ± 0.036
2.43GlyTyr: 2.43 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
2.53HisAla: 2.53 ± 0.034
0.203HisCys: 0.203 ± 0.009
1.337HisAsp: 1.337 ± 0.028
1.05HisGlu: 1.05 ± 0.019
0.573HisPhe: 0.573 ± 0.015
2.176HisGly: 2.176 ± 0.028
0.634HisHis: 0.634 ± 0.016
0.551HisIle: 0.551 ± 0.017
0.284HisLys: 0.284 ± 0.011
2.487HisLeu: 2.487 ± 0.034
0.263HisMet: 0.263 ± 0.011
0.382HisAsn: 0.382 ± 0.012
1.773HisPro: 1.773 ± 0.032
0.678HisGln: 0.678 ± 0.016
2.151HisArg: 2.151 ± 0.033
0.869HisSer: 0.869 ± 0.02
1.114HisThr: 1.114 ± 0.023
1.679HisVal: 1.679 ± 0.031
0.379HisTrp: 0.379 ± 0.013
0.457HisTyr: 0.457 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
4.849IleAla: 4.849 ± 0.045
0.298IleCys: 0.298 ± 0.011
2.386IleAsp: 2.386 ± 0.034
1.936IleGlu: 1.936 ± 0.03
0.876IlePhe: 0.876 ± 0.024
3.461IleGly: 3.461 ± 0.043
0.591IleHis: 0.591 ± 0.017
0.999IleIle: 0.999 ± 0.023
0.687IleLys: 0.687 ± 0.02
2.516IleLeu: 2.516 ± 0.038
0.452IleMet: 0.452 ± 0.013
0.782IleAsn: 0.782 ± 0.02
1.729IlePro: 1.729 ± 0.025
0.739IleGln: 0.739 ± 0.019
2.479IleArg: 2.479 ± 0.035
1.748IleSer: 1.748 ± 0.026
2.216IleThr: 2.216 ± 0.03
2.801IleVal: 2.801 ± 0.034
0.442IleTrp: 0.442 ± 0.013
0.643IleTyr: 0.643 ± 0.017
0.0IleXaa: 0.0 ± 0.0
Lys
2.308LysAla: 2.308 ± 0.036
0.099LysCys: 0.099 ± 0.006
0.957LysAsp: 0.957 ± 0.029
0.802LysGlu: 0.802 ± 0.023
0.426LysPhe: 0.426 ± 0.015
1.351LysGly: 1.351 ± 0.027
0.37LysHis: 0.37 ± 0.014
0.831LysIle: 0.831 ± 0.019
0.529LysLys: 0.529 ± 0.021
1.904LysLeu: 1.904 ± 0.035
0.31LysMet: 0.31 ± 0.012
0.419LysAsn: 0.419 ± 0.016
1.219LysPro: 1.219 ± 0.027
0.647LysGln: 0.647 ± 0.016
1.285LysArg: 1.285 ± 0.028
0.893LysSer: 0.893 ± 0.022
1.11LysThr: 1.11 ± 0.029
1.683LysVal: 1.683 ± 0.034
0.25LysTrp: 0.25 ± 0.01
0.394LysTyr: 0.394 ± 0.014
0.0LysXaa: 0.0 ± 0.0
Leu
16.931LeuAla: 16.931 ± 0.127
0.702LeuCys: 0.702 ± 0.016
6.893LeuAsp: 6.893 ± 0.065
3.87LeuGlu: 3.87 ± 0.047
2.609LeuPhe: 2.609 ± 0.041
9.301LeuGly: 9.301 ± 0.069
2.305LeuHis: 2.305 ± 0.037
3.388LeuIle: 3.388 ± 0.049
1.557LeuLys: 1.557 ± 0.031
11.82LeuLeu: 11.82 ± 0.105
1.42LeuMet: 1.42 ± 0.023
1.8LeuAsn: 1.8 ± 0.033
6.861LeuPro: 6.861 ± 0.058
2.112LeuGln: 2.112 ± 0.035
9.211LeuArg: 9.211 ± 0.075
4.897LeuSer: 4.897 ± 0.047
7.056LeuThr: 7.056 ± 0.068
9.805LeuVal: 9.805 ± 0.087
1.297LeuTrp: 1.297 ± 0.024
1.751LeuTyr: 1.751 ± 0.032
0.0LeuXaa: 0.0 ± 0.0
Met
2.108MetAla: 2.108 ± 0.027
0.103MetCys: 0.103 ± 0.006
0.718MetAsp: 0.718 ± 0.016
0.577MetGlu: 0.577 ± 0.015
0.464MetPhe: 0.464 ± 0.015
1.094MetGly: 1.094 ± 0.025
0.312MetHis: 0.312 ± 0.013
0.669MetIle: 0.669 ± 0.018
0.346MetLys: 0.346 ± 0.012
1.782MetLeu: 1.782 ± 0.027
0.259MetMet: 0.259 ± 0.011
0.367MetAsn: 0.367 ± 0.014
1.102MetPro: 1.102 ± 0.023
0.42MetGln: 0.42 ± 0.014
1.371MetArg: 1.371 ± 0.024
1.172MetSer: 1.172 ± 0.02
1.519MetThr: 1.519 ± 0.023
1.306MetVal: 1.306 ± 0.024
0.211MetTrp: 0.211 ± 0.008
0.288MetTyr: 0.288 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.258AsnAla: 2.258 ± 0.037
0.17AsnCys: 0.17 ± 0.008
1.017AsnAsp: 1.017 ± 0.022
0.82AsnGlu: 0.82 ± 0.021
0.531AsnPhe: 0.531 ± 0.013
1.938AsnGly: 1.938 ± 0.034
0.421AsnHis: 0.421 ± 0.013
0.635AsnIle: 0.635 ± 0.015
0.377AsnLys: 0.377 ± 0.016
1.922AsnLeu: 1.922 ± 0.036
0.29AsnMet: 0.29 ± 0.011
0.498AsnAsn: 0.498 ± 0.018
1.609AsnPro: 1.609 ± 0.032
0.618AsnGln: 0.618 ± 0.018
1.447AsnArg: 1.447 ± 0.024
0.9AsnSer: 0.9 ± 0.02
1.141AsnThr: 1.141 ± 0.027
1.438AsnVal: 1.438 ± 0.027
0.333AsnTrp: 0.333 ± 0.012
0.482AsnTyr: 0.482 ± 0.016
0.0AsnXaa: 0.0 ± 0.0
Pro
10.133ProAla: 10.133 ± 0.081
0.309ProCys: 0.309 ± 0.012
4.723ProAsp: 4.723 ± 0.051
3.817ProGlu: 3.817 ± 0.046
1.455ProPhe: 1.455 ± 0.025
6.658ProGly: 6.658 ± 0.066
1.226ProHis: 1.226 ± 0.024
1.687ProIle: 1.687 ± 0.025
1.076ProLys: 1.076 ± 0.025
5.338ProLeu: 5.338 ± 0.052
1.023ProMet: 1.023 ± 0.019
1.112ProAsn: 1.112 ± 0.023
4.227ProPro: 4.227 ± 0.071
1.714ProGln: 1.714 ± 0.032
3.987ProArg: 3.987 ± 0.041
3.003ProSer: 3.003 ± 0.041
3.906ProThr: 3.906 ± 0.045
5.91ProVal: 5.91 ± 0.051
0.951ProTrp: 0.951 ± 0.025
1.331ProTyr: 1.331 ± 0.024
0.0ProXaa: 0.0 ± 0.0
Gln
3.905GlnAla: 3.905 ± 0.041
0.163GlnCys: 0.163 ± 0.008
1.181GlnAsp: 1.181 ± 0.025
1.19GlnGlu: 1.19 ± 0.021
0.79GlnPhe: 0.79 ± 0.018
2.012GlnGly: 2.012 ± 0.029
0.674GlnHis: 0.674 ± 0.017
1.139GlnIle: 1.139 ± 0.022
0.492GlnLys: 0.492 ± 0.016
3.496GlnLeu: 3.496 ± 0.043
0.462GlnMet: 0.462 ± 0.015
0.495GlnAsn: 0.495 ± 0.017
2.053GlnPro: 2.053 ± 0.033
1.214GlnGln: 1.214 ± 0.024
2.827GlnArg: 2.827 ± 0.035
1.154GlnSer: 1.154 ± 0.026
1.371GlnThr: 1.371 ± 0.023
2.942GlnVal: 2.942 ± 0.033
0.489GlnTrp: 0.489 ± 0.013
0.593GlnTyr: 0.593 ± 0.015
0.0GlnXaa: 0.0 ± 0.0
Arg
10.157ArgAla: 10.157 ± 0.084
0.625ArgCys: 0.625 ± 0.015
4.59ArgAsp: 4.59 ± 0.044
4.23ArgGlu: 4.23 ± 0.045
2.411ArgPhe: 2.411 ± 0.035
5.674ArgGly: 5.674 ± 0.059
2.216ArgHis: 2.216 ± 0.031
3.209ArgIle: 3.209 ± 0.036
1.404ArgLys: 1.404 ± 0.027
9.591ArgLeu: 9.591 ± 0.083
1.854ArgMet: 1.854 ± 0.03
1.494ArgAsn: 1.494 ± 0.026
5.467ArgPro: 5.467 ± 0.055
2.806ArgGln: 2.806 ± 0.035
8.666ArgArg: 8.666 ± 0.085
4.008ArgSer: 4.008 ± 0.042
4.904ArgThr: 4.904 ± 0.053
6.305ArgVal: 6.305 ± 0.051
1.739ArgTrp: 1.739 ± 0.031
2.07ArgTyr: 2.07 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
6.165SerAla: 6.165 ± 0.052
0.405SerCys: 0.405 ± 0.015
2.392SerAsp: 2.392 ± 0.037
1.919SerGlu: 1.919 ± 0.033
1.474SerPhe: 1.474 ± 0.028
5.295SerGly: 5.295 ± 0.057
0.896SerHis: 0.896 ± 0.022
1.561SerIle: 1.561 ± 0.026
0.828SerLys: 0.828 ± 0.019
4.317SerLeu: 4.317 ± 0.043
0.982SerMet: 0.982 ± 0.02
0.908SerAsn: 0.908 ± 0.022
3.082SerPro: 3.082 ± 0.045
1.193SerGln: 1.193 ± 0.024
3.664SerArg: 3.664 ± 0.039
2.517SerSer: 2.517 ± 0.04
2.998SerThr: 2.998 ± 0.04
3.96SerVal: 3.96 ± 0.04
0.964SerTrp: 0.964 ± 0.019
1.221SerTyr: 1.221 ± 0.027
0.0SerXaa: 0.0 ± 0.0
Thr
8.66ThrAla: 8.66 ± 0.069
0.428ThrCys: 0.428 ± 0.014
3.721ThrAsp: 3.721 ± 0.049
2.928ThrGlu: 2.928 ± 0.037
1.689ThrPhe: 1.689 ± 0.024
6.662ThrGly: 6.662 ± 0.054
1.123ThrHis: 1.123 ± 0.02
2.012ThrIle: 2.012 ± 0.031
1.044ThrLys: 1.044 ± 0.028
5.759ThrLeu: 5.759 ± 0.051
0.957ThrMet: 0.957 ± 0.017
1.141ThrAsn: 1.141 ± 0.029
4.145ThrPro: 4.145 ± 0.043
1.314ThrGln: 1.314 ± 0.028
4.084ThrArg: 4.084 ± 0.043
2.975ThrSer: 2.975 ± 0.045
3.862ThrThr: 3.862 ± 0.069
6.256ThrVal: 6.256 ± 0.059
1.009ThrTrp: 1.009 ± 0.022
1.366ThrTyr: 1.366 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
12.588ValAla: 12.588 ± 0.087
0.664ValCys: 0.664 ± 0.016
5.912ValAsp: 5.912 ± 0.051
4.616ValGlu: 4.616 ± 0.055
2.296ValPhe: 2.296 ± 0.033
7.388ValGly: 7.388 ± 0.065
1.814ValHis: 1.814 ± 0.029
2.95ValIle: 2.95 ± 0.037
1.486ValLys: 1.486 ± 0.029
9.752ValLeu: 9.752 ± 0.086
1.148ValMet: 1.148 ± 0.02
1.766ValAsn: 1.766 ± 0.029
5.529ValPro: 5.529 ± 0.056
2.125ValGln: 2.125 ± 0.031
7.188ValArg: 7.188 ± 0.069
4.168ValSer: 4.168 ± 0.041
6.03ValThr: 6.03 ± 0.057
8.603ValVal: 8.603 ± 0.078
1.195ValTrp: 1.195 ± 0.022
1.554ValTyr: 1.554 ± 0.027
0.0ValXaa: 0.0 ± 0.0
Trp
1.809TrpAla: 1.809 ± 0.031
0.173TrpCys: 0.173 ± 0.008
0.814TrpAsp: 0.814 ± 0.016
0.624TrpGlu: 0.624 ± 0.015
0.529TrpPhe: 0.529 ± 0.015
1.018TrpGly: 1.018 ± 0.022
0.424TrpHis: 0.424 ± 0.013
0.573TrpIle: 0.573 ± 0.016
0.288TrpLys: 0.288 ± 0.011
2.1TrpLeu: 2.1 ± 0.032
0.29TrpMet: 0.29 ± 0.01
0.417TrpAsn: 0.417 ± 0.013
0.935TrpPro: 0.935 ± 0.021
0.7TrpGln: 0.7 ± 0.016
1.666TrpArg: 1.666 ± 0.027
1.058TrpSer: 1.058 ± 0.021
1.085TrpThr: 1.085 ± 0.022
1.149TrpVal: 1.149 ± 0.025
0.403TrpTrp: 0.403 ± 0.013
0.416TrpTyr: 0.416 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.914TyrAla: 2.914 ± 0.037
0.191TyrCys: 0.191 ± 0.011
1.537TyrAsp: 1.537 ± 0.039
1.043TyrGlu: 1.043 ± 0.019
0.619TyrPhe: 0.619 ± 0.015
2.136TyrGly: 2.136 ± 0.033
0.454TyrHis: 0.454 ± 0.016
0.444TyrIle: 0.444 ± 0.014
0.323TyrLys: 0.323 ± 0.012
2.354TyrLeu: 2.354 ± 0.031
0.199TyrMet: 0.199 ± 0.01
0.435TyrAsn: 0.435 ± 0.019
1.24TyrPro: 1.24 ± 0.021
0.765TyrGln: 0.765 ± 0.019
2.021TyrArg: 2.021 ± 0.031
0.911TyrSer: 0.911 ± 0.022
1.189TyrThr: 1.189 ± 0.025
1.775TyrVal: 1.775 ± 0.027
0.388TyrTrp: 0.388 ± 0.013
0.452TyrTyr: 0.452 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7693 proteins (2502689 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski