Birch leaf roll-associated virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Caulimoviridae; Badnavirus

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L0W0M5|A0A2L0W0M5_9VIRU Reverse transcriptase OS=Birch leaf roll-associated virus OX=2057979 GN=BLRaV_gp3 PE=4 SV=1
MM1 pKa = 7.42SANLSKK7 pKa = 10.88QPIQGEE13 pKa = 4.17DD14 pKa = 3.44DD15 pKa = 3.71YY16 pKa = 11.99EE17 pKa = 4.09EE18 pKa = 5.28YY19 pKa = 9.38ITNFQRR25 pKa = 11.84DD26 pKa = 3.63PRR28 pKa = 11.84ASRR31 pKa = 11.84LDD33 pKa = 3.72YY34 pKa = 11.29LDD36 pKa = 4.25LVYY39 pKa = 10.41PKK41 pKa = 9.52RR42 pKa = 11.84TEE44 pKa = 4.06LSTTAYY50 pKa = 8.06TIPCYY55 pKa = 10.14HH56 pKa = 6.88RR57 pKa = 11.84EE58 pKa = 3.78GRR60 pKa = 11.84LDD62 pKa = 3.28HH63 pKa = 6.72SAGHH67 pKa = 6.28SKK69 pKa = 9.35TDD71 pKa = 3.46PALNLLIDD79 pKa = 4.69LGFGLNDD86 pKa = 3.22NAAVAFDD93 pKa = 3.82RR94 pKa = 11.84AQLFSRR100 pKa = 11.84YY101 pKa = 9.23SLQRR105 pKa = 11.84FNTLEE110 pKa = 4.24DD111 pKa = 3.41KK112 pKa = 11.28VDD114 pKa = 3.27IVSQQVEE121 pKa = 4.36EE122 pKa = 3.92LRR124 pKa = 11.84KK125 pKa = 9.77EE126 pKa = 3.81IKK128 pKa = 10.41EE129 pKa = 4.12LVTVAPSKK137 pKa = 11.52GEE139 pKa = 3.86IPKK142 pKa = 10.27KK143 pKa = 9.32GQRR146 pKa = 11.84QPEE149 pKa = 4.31KK150 pKa = 9.19EE151 pKa = 4.23SLRR154 pKa = 11.84GVVDD158 pKa = 3.56QLEE161 pKa = 4.23KK162 pKa = 10.93QITQLQEE169 pKa = 3.64RR170 pKa = 11.84LSQVLEE176 pKa = 4.23KK177 pKa = 10.75EE178 pKa = 4.1KK179 pKa = 11.3SLDD182 pKa = 3.66TNIKK186 pKa = 8.61EE187 pKa = 4.26VKK189 pKa = 9.02EE190 pKa = 3.76LVRR193 pKa = 11.84ASII196 pKa = 4.0

Molecular weight: 22.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L0W0N7|A0A2L0W0N7_9VIRU ORF4 OS=Birch leaf roll-associated virus OX=2057979 GN=BLRaV_gp4 PE=4 SV=1
MM1 pKa = 7.63AKK3 pKa = 10.18SIQSTLDD10 pKa = 3.1SEE12 pKa = 5.14EE13 pKa = 3.99YY14 pKa = 10.44QRR16 pKa = 11.84TLDD19 pKa = 3.33ATKK22 pKa = 10.51VYY24 pKa = 9.37TEE26 pKa = 4.19KK27 pKa = 11.0NVGVGLSRR35 pKa = 11.84QDD37 pKa = 3.38VVVQSSLEE45 pKa = 3.9NFKK48 pKa = 11.18VVIAQNNSLIHH59 pKa = 6.48LVTTLARR66 pKa = 11.84RR67 pKa = 11.84VNDD70 pKa = 3.04QRR72 pKa = 11.84DD73 pKa = 3.56WITTLEE79 pKa = 4.14QKK81 pKa = 10.34VDD83 pKa = 3.61RR84 pKa = 11.84LTLAVEE90 pKa = 4.15KK91 pKa = 10.92GKK93 pKa = 8.51TPEE96 pKa = 3.79VALPDD101 pKa = 5.46DD102 pKa = 3.93ILEE105 pKa = 4.17GLIDD109 pKa = 4.25RR110 pKa = 11.84FSGIAIKK117 pKa = 10.62GGSGQKK123 pKa = 10.38KK124 pKa = 9.91KK125 pKa = 10.45IQTSQSYY132 pKa = 9.5HH133 pKa = 5.04VKK135 pKa = 9.98KK136 pKa = 10.62DD137 pKa = 3.14PYY139 pKa = 10.26EE140 pKa = 3.95ILRR143 pKa = 11.84EE144 pKa = 3.99EE145 pKa = 3.98QAKK148 pKa = 10.62LKK150 pKa = 10.08EE151 pKa = 4.32KK152 pKa = 10.63KK153 pKa = 10.21

Molecular weight: 17.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2486

153

1955

621.5

70.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.034 ± 0.736

1.488 ± 0.556

5.43 ± 0.483

8.447 ± 0.305

2.695 ± 0.557

6.718 ± 0.932

2.373 ± 0.364

6.235 ± 0.298

6.195 ± 1.304

7.804 ± 1.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.776 ± 0.701

3.982 ± 0.226

5.229 ± 0.708

5.39 ± 0.58

6.758 ± 1.63

6.436 ± 0.341

6.356 ± 0.302

4.706 ± 0.922

1.408 ± 0.443

3.54 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski