Tortoise microvirus 29
Average proteome isoelectric point is 7.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5T7|A0A4P8W5T7_9VIRU Replication initiation protein OS=Tortoise microvirus 29 OX=2583131 PE=4 SV=1
MM1 pKa = 6.85 QKK3 pKa = 10.18 KK4 pKa = 9.5 VKK6 pKa = 10.39 NVEE9 pKa = 3.88 QSSAPTVFVRR19 pKa = 11.84 KK20 pKa = 9.25 QLNYY24 pKa = 10.9 NLGDD28 pKa = 3.98 YY29 pKa = 9.39 ITDD32 pKa = 4.2 FDD34 pKa = 3.94 QSSGKK39 pKa = 10.2 SEE41 pKa = 4.11 TTPDD45 pKa = 3.29 QAKK48 pKa = 8.68 TPSEE52 pKa = 4.06 LVKK55 pKa = 10.53 LYY57 pKa = 10.7 YY58 pKa = 10.47 SGMSFAGGQTPQYY71 pKa = 10.42 SEE73 pKa = 5.76 LEE75 pKa = 4.06 IPDD78 pKa = 4.46 LKK80 pKa = 11.4 KK81 pKa = 9.81 MDD83 pKa = 4.11 LVEE86 pKa = 3.83 VDD88 pKa = 3.36 QYY90 pKa = 11.45 RR91 pKa = 11.84 EE92 pKa = 3.74 KK93 pKa = 10.91 LLKK96 pKa = 9.67 QRR98 pKa = 11.84 RR99 pKa = 11.84 EE100 pKa = 3.9 TEE102 pKa = 3.63 EE103 pKa = 3.69 ALRR106 pKa = 11.84 IASQEE111 pKa = 3.8 QAKK114 pKa = 10.52 KK115 pKa = 10.46 YY116 pKa = 10.2 DD117 pKa = 3.63 EE118 pKa = 4.17 EE119 pKa = 4.32 RR120 pKa = 11.84 QRR122 pKa = 11.84 QALEE126 pKa = 3.91 IIEE129 pKa = 4.53 EE130 pKa = 4.09 IKK132 pKa = 10.71 KK133 pKa = 10.6 RR134 pKa = 11.84 SSTQNNNNQNDD145 pKa = 3.97 PQTT148 pKa = 3.57
Molecular weight: 17.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.315
IPC2_protein 5.512
IPC_protein 5.334
Toseland 5.194
ProMoST 5.397
Dawson 5.258
Bjellqvist 5.397
Wikipedia 5.08
Rodwell 5.169
Grimsley 5.105
Solomon 5.245
Lehninger 5.207
Nozaki 5.359
DTASelect 5.461
Thurlkill 5.181
EMBOSS 5.105
Sillero 5.436
Patrickios 4.482
IPC_peptide 5.258
IPC2_peptide 5.423
IPC2.peptide.svr19 5.637
Protein with the highest isoelectric point:
>tr|A0A4V1FVX6|A0A4V1FVX6_9VIRU Major capsid protein OS=Tortoise microvirus 29 OX=2583131 PE=3 SV=1
MM1 pKa = 7.27 LQLSSRR7 pKa = 11.84 KK8 pKa = 9.28 FFFPYY13 pKa = 10.15 SPVRR17 pKa = 11.84 PLVVSLMQRR26 pKa = 11.84 GTNEE30 pKa = 3.51 ADD32 pKa = 3.53 SPRR35 pKa = 11.84 MQGRR39 pKa = 11.84 SGTEE43 pKa = 2.82 VDD45 pKa = 3.52 AANEE49 pKa = 3.89 RR50 pKa = 11.84 SEE52 pKa = 5.49 DD53 pKa = 3.81 KK54 pKa = 11.3 VSTTARR60 pKa = 11.84 KK61 pKa = 8.31 PAAGDD66 pKa = 3.56 GNPKK70 pKa = 10.1 VSTLYY75 pKa = 10.09 TT76 pKa = 3.47
Molecular weight: 8.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.008
IPC2_protein 9.048
IPC_protein 9.209
Toseland 9.823
ProMoST 9.516
Dawson 10.028
Bjellqvist 9.692
Wikipedia 10.204
Rodwell 10.335
Grimsley 10.101
Solomon 10.116
Lehninger 10.101
Nozaki 9.78
DTASelect 9.692
Thurlkill 9.882
EMBOSS 10.233
Sillero 9.94
Patrickios 10.16
IPC_peptide 10.116
IPC2_peptide 8.229
IPC2.peptide.svr19 8.025
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1313
76
496
262.6
29.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.626 ± 0.618
0.838 ± 0.497
5.331 ± 0.441
6.245 ± 0.935
4.113 ± 0.768
6.169 ± 0.889
2.056 ± 0.722
3.96 ± 0.42
7.159 ± 1.554
7.54 ± 0.905
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.123 ± 0.327
5.788 ± 0.614
5.179 ± 0.751
5.788 ± 1.218
5.712 ± 0.381
7.616 ± 0.473
5.255 ± 0.324
5.255 ± 0.941
1.219 ± 0.305
5.027 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here