Selenomonas noxia ATCC 43541
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2020 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D4S6T5|D4S6T5_9FIRM 50S ribosomal protein L7/L12 OS=Selenomonas noxia ATCC 43541 OX=585503 GN=rplL PE=3 SV=1
MM1 pKa = 7.83 PLLKK5 pKa = 10.57 EE6 pKa = 4.13 EE7 pKa = 4.34 NTMGMSGYY15 pKa = 10.47 YY16 pKa = 9.66 IAVDD20 pKa = 3.61 DD21 pKa = 5.54 ALLQDD26 pKa = 4.82 VIDD29 pKa = 4.62 GAQSVLGIDD38 pKa = 3.6 PSGEE42 pKa = 3.85 KK43 pKa = 9.72 TLDD46 pKa = 3.57 IDD48 pKa = 4.83 KK49 pKa = 10.17 SWQGIHH55 pKa = 6.16 QLLCGEE61 pKa = 4.6 PFEE64 pKa = 5.55 GDD66 pKa = 3.16 PPMGYY71 pKa = 9.63 VVPLQYY77 pKa = 11.08 DD78 pKa = 3.27 NRR80 pKa = 11.84 IEE82 pKa = 4.19 SEE84 pKa = 4.02 SDD86 pKa = 3.03 FGAFYY91 pKa = 9.35 ITPSEE96 pKa = 4.27 VKK98 pKa = 9.77 EE99 pKa = 4.0 AADD102 pKa = 4.05 FLSTLDD108 pKa = 4.5 DD109 pKa = 4.27 DD110 pKa = 5.32 ALRR113 pKa = 11.84 DD114 pKa = 3.69 MYY116 pKa = 11.31 GFDD119 pKa = 3.92 SMRR122 pKa = 11.84 GDD124 pKa = 3.21 GTYY127 pKa = 10.42 PITAEE132 pKa = 4.02 DD133 pKa = 3.99 DD134 pKa = 3.7 AEE136 pKa = 4.03 EE137 pKa = 4.81 FYY139 pKa = 11.15 SYY141 pKa = 10.86 LHH143 pKa = 6.75 SYY145 pKa = 10.37 LLEE148 pKa = 4.82 LISYY152 pKa = 7.47 FKK154 pKa = 11.09 KK155 pKa = 10.42 NAEE158 pKa = 3.93 EE159 pKa = 4.16 GKK161 pKa = 10.64 AVIFYY166 pKa = 10.58 ILL168 pKa = 3.5
Molecular weight: 18.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.735
IPC2_protein 3.834
IPC_protein 3.821
Toseland 3.617
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.656
Grimsley 3.528
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.139
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.948
Patrickios 0.807
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.818
Protein with the highest isoelectric point:
>tr|D4S3V5|D4S3V5_9FIRM Uncharacterized protein OS=Selenomonas noxia ATCC 43541 OX=585503 GN=HMPREF7545_0283 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNHH10 pKa = 5.38 WRR12 pKa = 11.84 KK13 pKa = 7.47 KK14 pKa = 6.28 THH16 pKa = 5.76 GFRR19 pKa = 11.84 EE20 pKa = 3.92 RR21 pKa = 11.84 MKK23 pKa = 10.1 TKK25 pKa = 10.35 GGRR28 pKa = 11.84 LVLKK32 pKa = 10.33 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.87 KK41 pKa = 10.62 LSAA44 pKa = 4.03
Molecular weight: 5.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.406
IPC2_protein 10.877
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2020
0
2020
628603
39
3482
311.2
34.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.823 ± 0.075
1.07 ± 0.021
5.57 ± 0.049
6.806 ± 0.061
3.803 ± 0.04
7.828 ± 0.054
2.229 ± 0.024
6.541 ± 0.052
4.422 ± 0.055
9.36 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.988 ± 0.031
3.233 ± 0.052
3.951 ± 0.033
3.021 ± 0.032
6.343 ± 0.062
5.208 ± 0.047
5.533 ± 0.053
7.064 ± 0.056
0.888 ± 0.02
3.321 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here