Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9140 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A1CI20|A1CI20_ASPCL 40S ribosomal protein S6 OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) OX=344612 GN=ACLA_049970 PE=3 SV=1
MM1 pKa = 7.75 ADD3 pKa = 3.62 EE4 pKa = 4.98 ALSIYY9 pKa = 10.61 DD10 pKa = 3.89 EE11 pKa = 4.51 IEE13 pKa = 4.22 IEE15 pKa = 5.63 DD16 pKa = 3.82 MVFDD20 pKa = 4.99 PNLQIYY26 pKa = 9.03 HH27 pKa = 5.7 YY28 pKa = 8.84 PCPCGDD34 pKa = 3.52 RR35 pKa = 11.84 FEE37 pKa = 4.77 IAIDD41 pKa = 3.76 DD42 pKa = 4.15 LRR44 pKa = 11.84 DD45 pKa = 3.75 GEE47 pKa = 5.2 DD48 pKa = 2.94 IAVCPSCSLMIRR60 pKa = 11.84 VIFDD64 pKa = 3.44 ASDD67 pKa = 3.39 LPKK70 pKa = 10.84 DD71 pKa = 3.87 GNQPVPGAVSVQAA84 pKa = 4.47
Molecular weight: 9.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.778
IPC2_protein 3.656
IPC_protein 3.617
Toseland 3.401
ProMoST 3.821
Dawson 3.63
Bjellqvist 3.795
Wikipedia 3.605
Rodwell 3.452
Grimsley 3.325
Solomon 3.605
Lehninger 3.567
Nozaki 3.757
DTASelect 3.999
Thurlkill 3.478
EMBOSS 3.605
Sillero 3.745
Patrickios 0.477
IPC_peptide 3.605
IPC2_peptide 3.719
IPC2.peptide.svr19 3.717
Protein with the highest isoelectric point:
>tr|A1C9Z0|A1C9Z0_ASPCL SUN domain-containing protein OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) OX=344612 GN=ACLA_009810 PE=4 SV=1
MM1 pKa = 7.28 TKK3 pKa = 10.45 GNKK6 pKa = 6.51 SHH8 pKa = 7.22 KK9 pKa = 10.13 SFRR12 pKa = 11.84 TKK14 pKa = 10.45 QKK16 pKa = 9.54 LAKK19 pKa = 9.74 AQRR22 pKa = 11.84 QNRR25 pKa = 11.84 PIPQWIRR32 pKa = 11.84 LRR34 pKa = 11.84 TGNTIRR40 pKa = 11.84 YY41 pKa = 5.79 NAKK44 pKa = 8.89 RR45 pKa = 11.84 RR46 pKa = 11.84 HH47 pKa = 4.14 WRR49 pKa = 11.84 KK50 pKa = 7.41 TRR52 pKa = 11.84 LGII55 pKa = 4.46
Molecular weight: 6.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9140
0
9140
4507684
42
8202
493.2
54.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.753 ± 0.025
1.19 ± 0.009
5.641 ± 0.015
6.238 ± 0.031
3.696 ± 0.015
6.724 ± 0.024
2.419 ± 0.01
4.861 ± 0.017
4.624 ± 0.025
9.136 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.143 ± 0.009
3.529 ± 0.012
6.113 ± 0.029
4.095 ± 0.015
6.277 ± 0.023
8.347 ± 0.028
5.853 ± 0.021
6.191 ± 0.017
1.413 ± 0.009
2.755 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here