Chlorobium phaeobacteroides (strain DSM 266)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae; Chlorobium/Pelodictyon group; Chlorobium; Chlorobium phaeobacteroides

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2579 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A1BDM2|A1BDM2_CHLPD Molybdenum ABC transporter periplasmic molybdate-binding protein OS=Chlorobium phaeobacteroides (strain DSM 266) OX=290317 GN=Cpha266_0441 PE=3 SV=1
MM1 pKa = 7.42KK2 pKa = 10.44KK3 pKa = 10.14SLSLLAVLAVVGFASTPVQAADD25 pKa = 3.95HH26 pKa = 6.15YY27 pKa = 8.93VSGMAGISWMQDD39 pKa = 2.66SSIDD43 pKa = 3.84LPLNLNEE50 pKa = 4.12VFGVGIDD57 pKa = 3.67LGYY60 pKa = 10.91SSGFTAVGAVGCDD73 pKa = 3.42YY74 pKa = 11.44GSTRR78 pKa = 11.84LEE80 pKa = 4.18AEE82 pKa = 4.01VGYY85 pKa = 7.84QTNDD89 pKa = 2.6IDD91 pKa = 5.4SITGSFDD98 pKa = 3.15GDD100 pKa = 3.97SASLALSGNAKK111 pKa = 9.97VLSLMANGFYY121 pKa = 10.8DD122 pKa = 3.85FDD124 pKa = 5.26LGGVEE129 pKa = 4.9LYY131 pKa = 11.3AMAGIGVAQVSINDD145 pKa = 3.56LTLADD150 pKa = 4.31TNDD153 pKa = 3.79LGISADD159 pKa = 4.97DD160 pKa = 3.46IDD162 pKa = 5.27DD163 pKa = 4.13ANEE166 pKa = 3.83YY167 pKa = 10.53LSRR170 pKa = 11.84RR171 pKa = 11.84LGAPVDD177 pKa = 4.42LNALGLNVNEE187 pKa = 4.18TTLAYY192 pKa = 9.93QVGAGLAVPIGDD204 pKa = 5.1GIMLDD209 pKa = 2.89ARR211 pKa = 11.84YY212 pKa = 9.76RR213 pKa = 11.84YY214 pKa = 8.81FATTDD219 pKa = 3.5FTMPLDD225 pKa = 4.15LNTNISSHH233 pKa = 5.66SALLGLRR240 pKa = 11.84VNLL243 pKa = 4.21

Molecular weight: 25.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A1BCI7|A1BCI7_CHLPD Geranylgeranyl reductase OS=Chlorobium phaeobacteroides (strain DSM 266) OX=290317 GN=Cpha266_0044 PE=3 SV=1
MM1 pKa = 7.33KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.66QPRR8 pKa = 11.84NRR10 pKa = 11.84KK11 pKa = 8.8RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.88HH16 pKa = 4.47GFRR19 pKa = 11.84QRR21 pKa = 11.84MSTKK25 pKa = 9.43NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.14GRR39 pKa = 11.84HH40 pKa = 4.91SLSVSSAMGTAGQQ53 pKa = 3.64

Molecular weight: 6.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2579

0

2579

851888

38

4876

330.3

36.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.298 ± 0.056

1.142 ± 0.024

5.379 ± 0.063

6.628 ± 0.051

4.452 ± 0.041

7.379 ± 0.136

2.118 ± 0.026

6.634 ± 0.041

5.471 ± 0.058

10.198 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.517 ± 0.029

3.83 ± 0.049

4.126 ± 0.041

3.289 ± 0.031

5.693 ± 0.055

6.804 ± 0.058

5.201 ± 0.065

6.691 ± 0.044

1.061 ± 0.019

3.088 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski