Massilia sp. JS1662

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Massilia; unclassified Massilia

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5560 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A098U8U5|A0A098U8U5_9BURK Uncharacterized protein OS=Massilia sp. JS1662 OX=1519190 GN=IA69_16140 PE=4 SV=1
MM1 pKa = 7.12NAVAEE6 pKa = 4.33VQNAQDD12 pKa = 4.52TIPAPINFTDD22 pKa = 3.59SAAQKK27 pKa = 9.24VAQLIEE33 pKa = 4.37EE34 pKa = 4.51EE35 pKa = 4.63GNPDD39 pKa = 2.85LKK41 pKa = 11.13LRR43 pKa = 11.84VFVQGGGCSGFQYY56 pKa = 10.94GFTFDD61 pKa = 4.69EE62 pKa = 4.93IVNEE66 pKa = 4.96DD67 pKa = 3.85DD68 pKa = 3.19TTMEE72 pKa = 4.19KK73 pKa = 10.82NGVQLLIDD81 pKa = 3.77SMSYY85 pKa = 10.23QYY87 pKa = 11.55LVGAEE92 pKa = 4.01IDD94 pKa = 3.89YY95 pKa = 11.28KK96 pKa = 11.39DD97 pKa = 4.47DD98 pKa = 4.08LEE100 pKa = 4.56GAQFVIKK107 pKa = 10.56NPNAQSTCGCGSSFSMM123 pKa = 5.09

Molecular weight: 13.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A098U9G6|A0A098U9G6_9BURK Uncharacterized protein OS=Massilia sp. JS1662 OX=1519190 GN=IA69_14280 PE=4 SV=1
MM1 pKa = 6.97LTSPAFFRR9 pKa = 11.84KK10 pKa = 9.36SRR12 pKa = 11.84LDD14 pKa = 3.36MRR16 pKa = 11.84QLRR19 pKa = 11.84LLVALDD25 pKa = 2.9THH27 pKa = 7.01RR28 pKa = 11.84NLRR31 pKa = 11.84LAAQAMCVTQPGASTLLKK49 pKa = 10.04TIEE52 pKa = 4.26TTLGVPLFEE61 pKa = 4.77RR62 pKa = 11.84MPRR65 pKa = 11.84PMAPTQYY72 pKa = 9.39GEE74 pKa = 4.14TVIRR78 pKa = 11.84HH79 pKa = 5.28ARR81 pKa = 11.84MTIASLGRR89 pKa = 11.84GLASLEE95 pKa = 4.07SLRR98 pKa = 11.84AGATGKK104 pKa = 10.37VLVGSIMTPALTLLPRR120 pKa = 11.84AIADD124 pKa = 3.52VKK126 pKa = 10.88ARR128 pKa = 11.84APRR131 pKa = 11.84LHH133 pKa = 7.28VGVHH137 pKa = 5.49TDD139 pKa = 2.5SSEE142 pKa = 4.04RR143 pKa = 11.84LFEE146 pKa = 3.97MLEE149 pKa = 3.98RR150 pKa = 11.84GAVDD154 pKa = 3.93FMVGRR159 pKa = 11.84LPKK162 pKa = 9.99QVAVAAWCYY171 pKa = 10.56EE172 pKa = 3.85PLADD176 pKa = 3.67EE177 pKa = 4.56HH178 pKa = 7.24ACVVVRR184 pKa = 11.84VGHH187 pKa = 6.48PLLDD191 pKa = 3.47RR192 pKa = 11.84RR193 pKa = 11.84GLKK196 pKa = 9.54LAHH199 pKa = 6.75LAALAWILPKK209 pKa = 10.34RR210 pKa = 11.84GNVLRR215 pKa = 11.84EE216 pKa = 3.57RR217 pKa = 11.84FDD219 pKa = 3.73AVFAATGVPAPRR231 pKa = 11.84NVVEE235 pKa = 4.57ADD237 pKa = 3.22APLVVTSLLQHH248 pKa = 6.09TDD250 pKa = 3.03ALNVMPLEE258 pKa = 4.35VARR261 pKa = 11.84HH262 pKa = 4.92SEE264 pKa = 3.85AMGLLRR270 pKa = 11.84ILPLALPCTLAQFGLAYY287 pKa = 10.19AANRR291 pKa = 11.84PLSPAAALMLNAARR305 pKa = 11.84AAAGRR310 pKa = 11.84TTALKK315 pKa = 10.22PP316 pKa = 3.43

Molecular weight: 34.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5560

0

5560

1865432

32

2890

335.5

36.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.762 ± 0.051

0.834 ± 0.01

5.843 ± 0.025

4.916 ± 0.031

3.589 ± 0.021

8.496 ± 0.037

2.227 ± 0.016

4.46 ± 0.022

3.36 ± 0.028

10.191 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.41 ± 0.015

3.0 ± 0.027

5.193 ± 0.026

3.601 ± 0.022

6.87 ± 0.037

5.006 ± 0.025

5.525 ± 0.026

7.679 ± 0.026

1.441 ± 0.015

2.596 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski