Trypanosoma congolense (strain IL3000)
Average proteome isoelectric point is 7.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5906 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F9WIH8|F9WIH8_TRYCI WGS project CAEQ00000000 data annotated contig 794 OS=Trypanosoma congolense (strain IL3000) OX=1068625 GN=TCIL3000_0_19740 PE=4 SV=1
MM1 pKa = 7.67 SLRR4 pKa = 11.84 AQWNMKK10 pKa = 9.63 EE11 pKa = 4.25 GSFAGNAGGIDD22 pKa = 3.71 SPNDD26 pKa = 3.27 CSSASGIMDD35 pKa = 4.66 GLHH38 pKa = 7.33 LIPEE42 pKa = 4.23 QGVVDD47 pKa = 4.26 ALQPVAHH54 pKa = 7.34 AGQAEE59 pKa = 4.01 NSADD63 pKa = 3.48 GALSFDD69 pKa = 5.2 FIFHH73 pKa = 6.73 SMQPNDD79 pKa = 3.18 VNRR82 pKa = 11.84 DD83 pKa = 3.22 VNANCVASEE92 pKa = 4.11 GDD94 pKa = 3.71 PSRR97 pKa = 11.84 STAFGGFCFDD107 pKa = 4.07 YY108 pKa = 10.84 ACSDD112 pKa = 3.89 GAWPEE117 pKa = 4.07 GNAVATDD124 pKa = 3.96 AAAMGVGINNPTTQEE139 pKa = 4.07 APFHH143 pKa = 6.59 LLLTDD148 pKa = 4.6 DD149 pKa = 4.57 PVVPCDD155 pKa = 3.72 EE156 pKa = 4.64 PLHH159 pKa = 6.24 PGFPKK164 pKa = 10.67 ASS166 pKa = 3.39
Molecular weight: 17.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.773
IPC2_protein 3.973
IPC_protein 3.948
Toseland 3.732
ProMoST 4.126
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.897
Rodwell 3.783
Grimsley 3.643
Solomon 3.935
Lehninger 3.897
Nozaki 4.075
DTASelect 4.317
Thurlkill 3.795
EMBOSS 3.91
Sillero 4.075
Patrickios 1.939
IPC_peptide 3.935
IPC2_peptide 4.05
IPC2.peptide.svr19 3.959
Protein with the highest isoelectric point:
>tr|F9WCD2|F9WCD2_TRYCI WGS project CAEQ00000000 data annotated contig 2213 OS=Trypanosoma congolense (strain IL3000) OX=1068625 GN=TCIL3000_0_54940 PE=4 SV=1
MM1 pKa = 7.91 APPFIPPHH9 pKa = 6.21 PSNGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLRR301 pKa = 11.84 LGLGLGLRR309 pKa = 11.84 LPLRR313 pKa = 11.84 LGFGLGLRR321 pKa = 11.84 VRR323 pKa = 11.84 VRR325 pKa = 11.84 LRR327 pKa = 11.84 VRR329 pKa = 11.84 LRR331 pKa = 11.84 LTVRR335 pKa = 11.84 VRR337 pKa = 11.84 LL338 pKa = 3.85
Molecular weight: 30.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.228
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.501
Grimsley 13.042
Solomon 13.495
Lehninger 13.393
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.237
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5906
0
5906
2211927
11
4553
374.5
41.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.967 ± 0.048
2.539 ± 0.029
4.794 ± 0.029
6.729 ± 0.058
3.651 ± 0.031
6.802 ± 0.037
2.779 ± 0.037
4.237 ± 0.045
5.054 ± 0.038
9.196 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.48 ± 0.038
3.711 ± 0.031
4.966 ± 0.038
3.705 ± 0.049
6.582 ± 0.039
7.904 ± 0.048
5.612 ± 0.037
7.284 ± 0.045
1.405 ± 0.015
2.593 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here