Aeromicrobium sp. Root344

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Aeromicrobium; unclassified Aeromicrobium

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3738 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q6VSF3|A0A0Q6VSF3_9ACTN Uncharacterized protein OS=Aeromicrobium sp. Root344 OX=1736521 GN=ASC61_07745 PE=4 SV=1
MM1 pKa = 7.41RR2 pKa = 11.84AVRR5 pKa = 11.84GLFALLLAVILSLTFTAAHH24 pKa = 7.02ADD26 pKa = 3.79DD27 pKa = 5.59DD28 pKa = 4.93PGGGTPEE35 pKa = 4.05TSQTPEE41 pKa = 4.42PSDD44 pKa = 3.67QPTDD48 pKa = 3.62PAPTPTEE55 pKa = 4.2TTPSPSEE62 pKa = 3.82PTPTEE67 pKa = 3.98TTPTPSEE74 pKa = 4.19PKK76 pKa = 10.07PSDD79 pKa = 3.26GTPTGEE85 pKa = 4.34PGVEE89 pKa = 4.13PTDD92 pKa = 3.95QPTKK96 pKa = 9.89PADD99 pKa = 3.75RR100 pKa = 11.84PGRR103 pKa = 11.84DD104 pKa = 3.09ATVKK108 pKa = 10.49AKK110 pKa = 9.95PAIEE114 pKa = 4.21GFVAEE119 pKa = 4.71IAAALAAPVANDD131 pKa = 3.55DD132 pKa = 3.89VFSVVTNTGAFPSNIGLLRR151 pKa = 11.84PGIIGVYY158 pKa = 10.23RR159 pKa = 11.84NDD161 pKa = 3.13VPTAGGDD168 pKa = 3.34QTPNSIVLMNDD179 pKa = 2.66VDD181 pKa = 5.35HH182 pKa = 6.84GTLDD186 pKa = 3.74LNGDD190 pKa = 3.9GGFHH194 pKa = 5.9YY195 pKa = 10.65VPEE198 pKa = 4.24VGYY201 pKa = 10.81SGTDD205 pKa = 3.02TFDD208 pKa = 2.96YY209 pKa = 10.66RR210 pKa = 11.84YY211 pKa = 8.97ATDD214 pKa = 4.46DD215 pKa = 4.17GEE217 pKa = 5.33SNTATVTIVVGNGGLIATDD236 pKa = 3.49DD237 pKa = 4.38HH238 pKa = 7.11YY239 pKa = 11.79VTAPDD244 pKa = 3.18TALQVSVPGVSSNDD258 pKa = 3.25SEE260 pKa = 4.02IAYY263 pKa = 10.49LGFQVTNNVDD273 pKa = 3.46HH274 pKa = 6.72GTLTQPGFLPSGGFTYY290 pKa = 10.39TPDD293 pKa = 3.63PGFVGVDD300 pKa = 3.14TFTYY304 pKa = 9.98RR305 pKa = 11.84YY306 pKa = 9.35RR307 pKa = 11.84PLPFGPYY314 pKa = 10.09SNTATVTIIVGEE326 pKa = 4.13PPVAEE331 pKa = 4.77DD332 pKa = 3.69DD333 pKa = 4.39AYY335 pKa = 11.54SIAANSALHH344 pKa = 4.8VTAPGVRR351 pKa = 11.84DD352 pKa = 3.57NDD354 pKa = 3.9TGTGTVAVTGDD365 pKa = 3.67VSHH368 pKa = 6.45GTLDD372 pKa = 3.42LHH374 pKa = 6.8TDD376 pKa = 3.38GSFDD380 pKa = 3.81YY381 pKa = 11.12APTADD386 pKa = 4.0FVGVDD391 pKa = 2.99SFTYY395 pKa = 10.48RR396 pKa = 11.84FTDD399 pKa = 3.5PVTTLSDD406 pKa = 3.5VATVTLRR413 pKa = 11.84VTPVAADD420 pKa = 3.67DD421 pKa = 4.98AYY423 pKa = 10.05QTNAEE428 pKa = 4.11STLNVNAPGVFGNDD442 pKa = 3.21TPTSGGTPSVTDD454 pKa = 4.1DD455 pKa = 3.87VDD457 pKa = 5.77DD458 pKa = 4.44GTLTLNDD465 pKa = 4.94DD466 pKa = 3.91GSFTYY471 pKa = 10.43TPDD474 pKa = 3.12AGFAGVDD481 pKa = 3.31TFSYY485 pKa = 10.48SFQSTEE491 pKa = 3.69LDD493 pKa = 3.76SNVATVTITVSPVAVDD509 pKa = 4.49DD510 pKa = 4.25EE511 pKa = 5.26VSAQQCDD518 pKa = 3.62DD519 pKa = 3.57RR520 pKa = 11.84TFTPLANDD528 pKa = 3.84AGVDD532 pKa = 3.72PLGSVPTIIAPPEE545 pKa = 4.61HH546 pKa = 6.37GTLTYY551 pKa = 10.62PEE553 pKa = 4.89DD554 pKa = 3.67LAGGALIYY562 pKa = 11.02DD563 pKa = 4.31PDD565 pKa = 4.96DD566 pKa = 5.11DD567 pKa = 5.27FVGDD571 pKa = 3.82DD572 pKa = 3.29TFTYY576 pKa = 10.07TFATQGVVSNAATVTIHH593 pKa = 5.29VTACEE598 pKa = 4.22SGDD601 pKa = 4.0GGNSDD606 pKa = 5.53DD607 pKa = 5.19GDD609 pKa = 3.7EE610 pKa = 4.66ALPDD614 pKa = 3.73TGSPASPWLLLVAGFATFAGVVLIRR639 pKa = 11.84RR640 pKa = 11.84RR641 pKa = 11.84GGAQAA646 pKa = 4.25

Molecular weight: 66.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q6V7Y8|A0A0Q6V7Y8_9ACTN Acyl-CoA dehydrogenase OS=Aeromicrobium sp. Root344 OX=1736521 GN=ASC61_07670 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.97KK16 pKa = 9.33HH17 pKa = 4.25GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILADD34 pKa = 3.74RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.84GRR40 pKa = 11.84AKK42 pKa = 10.68LSAA45 pKa = 3.92

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3738

0

3738

1174455

37

2996

314.2

33.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.547 ± 0.056

0.654 ± 0.011

6.603 ± 0.033

5.602 ± 0.04

3.067 ± 0.023

8.912 ± 0.033

2.168 ± 0.02

4.394 ± 0.024

2.591 ± 0.035

9.98 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.97 ± 0.014

2.027 ± 0.023

5.174 ± 0.031

2.815 ± 0.021

6.903 ± 0.041

5.672 ± 0.029

6.281 ± 0.031

9.044 ± 0.044

1.51 ± 0.018

2.087 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski