Amino acid dipepetide frequency for Aeromicrobium sp. Root344

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.61AlaAla: 17.61 ± 0.183	0.904AlaCys: 0.904 ± 0.029	8.129AlaAsp: 8.129 ± 0.092	7.762AlaGlu: 7.762 ± 0.115	3.753AlaPhe: 3.753 ± 0.054	11.526AlaGly: 11.526 ± 0.121	2.438AlaHis: 2.438 ± 0.05	5.716AlaIle: 5.716 ± 0.081	3.55AlaLys: 3.55 ± 0.065	12.556AlaLeu: 12.556 ± 0.121	2.859AlaMet: 2.859 ± 0.057	2.3AlaAsn: 2.3 ± 0.04	5.286AlaPro: 5.286 ± 0.081	3.518AlaGln: 3.518 ± 0.063	8.043AlaArg: 8.043 ± 0.101	6.742AlaSer: 6.742 ± 0.093	7.288AlaThr: 7.288 ± 0.084	10.737AlaVal: 10.737 ± 0.124	1.845AlaTrp: 1.845 ± 0.044	2.912AlaTyr: 2.912 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.78CysAla: 0.78 ± 0.029	0.072CysCys: 0.072 ± 0.008	0.402CysAsp: 0.402 ± 0.019	0.363CysGlu: 0.363 ± 0.019	0.205CysPhe: 0.205 ± 0.014	0.755CysGly: 0.755 ± 0.024	0.163CysHis: 0.163 ± 0.013	0.213CysIle: 0.213 ± 0.013	0.134CysLys: 0.134 ± 0.011	0.614CysLeu: 0.614 ± 0.021	0.118CysMet: 0.118 ± 0.009	0.134CysAsn: 0.134 ± 0.01	0.38CysPro: 0.38 ± 0.02	0.157CysGln: 0.157 ± 0.012	0.444CysArg: 0.444 ± 0.017	0.398CysSer: 0.398 ± 0.019	0.431CysThr: 0.431 ± 0.02	0.568CysVal: 0.568 ± 0.024	0.087CysTrp: 0.087 ± 0.01	0.12CysTyr: 0.12 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.935AspAla: 7.935 ± 0.095	0.331AspCys: 0.331 ± 0.017	4.842AspAsp: 4.842 ± 0.08	4.649AspGlu: 4.649 ± 0.067	1.842AspPhe: 1.842 ± 0.043	6.291AspGly: 6.291 ± 0.091	1.599AspHis: 1.599 ± 0.039	2.572AspIle: 2.572 ± 0.044	1.654AspLys: 1.654 ± 0.045	6.938AspLeu: 6.938 ± 0.073	0.938AspMet: 0.938 ± 0.028	1.309AspAsn: 1.309 ± 0.037	4.247AspPro: 4.247 ± 0.066	1.852AspGln: 1.852 ± 0.039	4.555AspArg: 4.555 ± 0.063	2.88AspSer: 2.88 ± 0.052	3.154AspThr: 3.154 ± 0.057	6.102AspVal: 6.102 ± 0.073	1.003AspTrp: 1.003 ± 0.026	1.334AspTyr: 1.334 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	7.033GluAla: 7.033 ± 0.102	0.294GluCys: 0.294 ± 0.016	2.93GluAsp: 2.93 ± 0.06	2.809GluGlu: 2.809 ± 0.063	1.699GluPhe: 1.699 ± 0.042	4.153GluGly: 4.153 ± 0.061	1.556GluHis: 1.556 ± 0.04	2.825GluIle: 2.825 ± 0.059	1.404GluLys: 1.404 ± 0.043	6.6GluLeu: 6.6 ± 0.083	1.052GluMet: 1.052 ± 0.033	0.983GluAsn: 0.983 ± 0.025	3.112GluPro: 3.112 ± 0.055	2.238GluGln: 2.238 ± 0.044	4.719GluArg: 4.719 ± 0.083	3.041GluSer: 3.041 ± 0.054	3.02GluThr: 3.02 ± 0.06	4.794GluVal: 4.794 ± 0.076	0.804GluTrp: 0.804 ± 0.022	0.953GluTyr: 0.953 ± 0.028	0.001GluXaa: 0.001 ± 0.001
Phe	4.042PheAla: 4.042 ± 0.061	0.254PheCys: 0.254 ± 0.016	2.405PheAsp: 2.405 ± 0.04	1.705PheGlu: 1.705 ± 0.042	1.02PhePhe: 1.02 ± 0.032	3.313PheGly: 3.313 ± 0.056	0.632PheHis: 0.632 ± 0.021	1.1PheIle: 1.1 ± 0.031	0.674PheLys: 0.674 ± 0.025	2.657PheLeu: 2.657 ± 0.052	0.532PheMet: 0.532 ± 0.021	0.711PheAsn: 0.711 ± 0.026	1.344PhePro: 1.344 ± 0.034	0.696PheGln: 0.696 ± 0.026	1.722PheArg: 1.722 ± 0.036	1.736PheSer: 1.736 ± 0.043	2.108PheThr: 2.108 ± 0.047	2.85PheVal: 2.85 ± 0.053	0.47PheTrp: 0.47 ± 0.021	0.703PheTyr: 0.703 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	9.719GlyAla: 9.719 ± 0.101	0.655GlyCys: 0.655 ± 0.026	5.447GlyAsp: 5.447 ± 0.071	4.597GlyGlu: 4.597 ± 0.064	3.315GlyPhe: 3.315 ± 0.053	7.764GlyGly: 7.764 ± 0.111	1.966GlyHis: 1.966 ± 0.042	4.38GlyIle: 4.38 ± 0.065	2.693GlyLys: 2.693 ± 0.051	9.141GlyLeu: 9.141 ± 0.102	1.958GlyMet: 1.958 ± 0.048	1.878GlyAsn: 1.878 ± 0.043	3.962GlyPro: 3.962 ± 0.059	2.529GlyGln: 2.529 ± 0.048	6.223GlyArg: 6.223 ± 0.072	5.631GlySer: 5.631 ± 0.078	5.705GlyThr: 5.705 ± 0.087	7.776GlyVal: 7.776 ± 0.085	1.618GlyTrp: 1.618 ± 0.04	2.164GlyTyr: 2.164 ± 0.047	0.001GlyXaa: 0.001 ± 0.001
His	2.422HisAla: 2.422 ± 0.05	0.157HisCys: 0.157 ± 0.011	1.578HisAsp: 1.578 ± 0.036	1.309HisGlu: 1.309 ± 0.034	0.621HisPhe: 0.621 ± 0.022	2.151HisGly: 2.151 ± 0.044	0.656HisHis: 0.656 ± 0.025	0.714HisIle: 0.714 ± 0.024	0.378HisLys: 0.378 ± 0.018	2.27HisLeu: 2.27 ± 0.044	0.371HisMet: 0.371 ± 0.017	0.403HisAsn: 0.403 ± 0.02	1.434HisPro: 1.434 ± 0.035	0.622HisGln: 0.622 ± 0.027	1.63HisArg: 1.63 ± 0.036	0.929HisSer: 0.929 ± 0.03	1.168HisThr: 1.168 ± 0.031	2.037HisVal: 2.037 ± 0.045	0.33HisTrp: 0.33 ± 0.017	0.496HisTyr: 0.496 ± 0.021	0.001HisXaa: 0.001 ± 0.001
Ile	6.475IleAla: 6.475 ± 0.076	0.328IleCys: 0.328 ± 0.017	3.519IleAsp: 3.519 ± 0.062	2.841IleGlu: 2.841 ± 0.051	1.119IlePhe: 1.119 ± 0.033	4.648IleGly: 4.648 ± 0.074	0.843IleHis: 0.843 ± 0.026	1.592IleIle: 1.592 ± 0.036	1.124IleLys: 1.124 ± 0.031	3.33IleLeu: 3.33 ± 0.055	0.654IleMet: 0.654 ± 0.026	1.037IleAsn: 1.037 ± 0.034	2.112IlePro: 2.112 ± 0.044	0.955IleGln: 0.955 ± 0.027	2.587IleArg: 2.587 ± 0.047	2.372IleSer: 2.372 ± 0.042	2.831IleThr: 2.831 ± 0.046	4.316IleVal: 4.316 ± 0.07	0.504IleTrp: 0.504 ± 0.021	0.752IleTyr: 0.752 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	3.446LysAla: 3.446 ± 0.072	0.112LysCys: 0.112 ± 0.009	1.649LysAsp: 1.649 ± 0.044	1.224LysGlu: 1.224 ± 0.041	0.624LysPhe: 0.624 ± 0.023	2.174LysGly: 2.174 ± 0.052	0.592LysHis: 0.592 ± 0.021	1.162LysIle: 1.162 ± 0.035	1.078LysLys: 1.078 ± 0.046	2.345LysLeu: 2.345 ± 0.054	0.438LysMet: 0.438 ± 0.023	0.615LysAsn: 0.615 ± 0.025	1.615LysPro: 1.615 ± 0.039	0.894LysGln: 0.894 ± 0.031	1.646LysArg: 1.646 ± 0.044	1.471LysSer: 1.471 ± 0.041	1.569LysThr: 1.569 ± 0.045	2.468LysVal: 2.468 ± 0.05	0.268LysTrp: 0.268 ± 0.015	0.522LysTyr: 0.522 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	13.674LeuAla: 13.674 ± 0.125	0.655LeuCys: 0.655 ± 0.024	7.251LeuAsp: 7.251 ± 0.087	5.356LeuGlu: 5.356 ± 0.074	2.608LeuPhe: 2.608 ± 0.058	9.234LeuGly: 9.234 ± 0.103	2.011LeuHis: 2.011 ± 0.045	4.09LeuIle: 4.09 ± 0.074	2.334LeuLys: 2.334 ± 0.053	9.87LeuLeu: 9.87 ± 0.122	1.825LeuMet: 1.825 ± 0.045	1.846LeuAsn: 1.846 ± 0.041	5.196LeuPro: 5.196 ± 0.073	2.44LeuGln: 2.44 ± 0.047	6.957LeuArg: 6.957 ± 0.086	5.783LeuSer: 5.783 ± 0.066	6.4LeuThr: 6.4 ± 0.081	9.555LeuVal: 9.555 ± 0.119	1.153LeuTrp: 1.153 ± 0.033	1.562LeuTyr: 1.562 ± 0.038	0.0LeuXaa: 0.0 ± 0.0
Met	2.359MetAla: 2.359 ± 0.05	0.125MetCys: 0.125 ± 0.01	0.915MetAsp: 0.915 ± 0.031	0.717MetGlu: 0.717 ± 0.027	0.578MetPhe: 0.578 ± 0.025	1.435MetGly: 1.435 ± 0.039	0.412MetHis: 0.412 ± 0.017	0.895MetIle: 0.895 ± 0.027	0.574MetLys: 0.574 ± 0.021	1.948MetLeu: 1.948 ± 0.041	0.345MetMet: 0.345 ± 0.019	0.494MetAsn: 0.494 ± 0.023	1.186MetPro: 1.186 ± 0.029	0.576MetGln: 0.576 ± 0.023	1.489MetArg: 1.489 ± 0.036	1.534MetSer: 1.534 ± 0.035	2.026MetThr: 2.026 ± 0.045	1.523MetVal: 1.523 ± 0.032	0.238MetTrp: 0.238 ± 0.015	0.33MetTyr: 0.33 ± 0.017	0.001MetXaa: 0.001 ± 0.001
Asn	2.515AsnAla: 2.515 ± 0.048	0.136AsnCys: 0.136 ± 0.012	1.334AsnAsp: 1.334 ± 0.033	0.99AsnGlu: 0.99 ± 0.029	0.598AsnPhe: 0.598 ± 0.024	1.938AsnGly: 1.938 ± 0.054	0.435AsnHis: 0.435 ± 0.022	0.879AsnIle: 0.879 ± 0.027	0.496AsnLys: 0.496 ± 0.024	1.986AsnLeu: 1.986 ± 0.043	0.312AsnMet: 0.312 ± 0.015	0.499AsnAsn: 0.499 ± 0.022	1.461AsnPro: 1.461 ± 0.03	0.636AsnGln: 0.636 ± 0.024	1.327AsnArg: 1.327 ± 0.036	1.0AsnSer: 1.0 ± 0.033	1.166AsnThr: 1.166 ± 0.037	1.801AsnVal: 1.801 ± 0.034	0.3AsnTrp: 0.3 ± 0.016	0.461AsnTyr: 0.461 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	6.325ProAla: 6.325 ± 0.089	0.24ProCys: 0.24 ± 0.016	4.17ProAsp: 4.17 ± 0.066	3.641ProGlu: 3.641 ± 0.056	1.593ProPhe: 1.593 ± 0.036	4.919ProGly: 4.919 ± 0.077	1.166ProHis: 1.166 ± 0.031	1.915ProIle: 1.915 ± 0.041	1.348ProLys: 1.348 ± 0.037	4.561ProLeu: 4.561 ± 0.065	1.04ProMet: 1.04 ± 0.028	0.96ProAsn: 0.96 ± 0.029	2.386ProPro: 2.386 ± 0.053	1.354ProGln: 1.354 ± 0.032	3.134ProArg: 3.134 ± 0.056	3.018ProSer: 3.018 ± 0.047	3.313ProThr: 3.313 ± 0.061	4.577ProVal: 4.577 ± 0.069	0.891ProTrp: 0.891 ± 0.029	1.189ProTyr: 1.189 ± 0.032	0.002ProXaa: 0.002 ± 0.002
Gln	3.516GlnAla: 3.516 ± 0.057	0.151GlnCys: 0.151 ± 0.012	1.396GlnAsp: 1.396 ± 0.038	1.218GlnGlu: 1.218 ± 0.031	0.837GlnPhe: 0.837 ± 0.026	2.043GlnGly: 2.043 ± 0.041	0.679GlnHis: 0.679 ± 0.024	1.384GlnIle: 1.384 ± 0.041	0.756GlnLys: 0.756 ± 0.026	3.233GlnLeu: 3.233 ± 0.055	0.616GlnMet: 0.616 ± 0.024	0.544GlnAsn: 0.544 ± 0.024	1.532GlnPro: 1.532 ± 0.037	1.159GlnGln: 1.159 ± 0.036	2.347GlnArg: 2.347 ± 0.058	1.462GlnSer: 1.462 ± 0.034	1.509GlnThr: 1.509 ± 0.034	2.711GlnVal: 2.711 ± 0.05	0.484GlnTrp: 0.484 ± 0.019	0.57GlnTyr: 0.57 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	7.609ArgAla: 7.609 ± 0.087	0.386ArgCys: 0.386 ± 0.019	4.21ArgAsp: 4.21 ± 0.059	4.06ArgGlu: 4.06 ± 0.069	2.277ArgPhe: 2.277 ± 0.045	4.91ArgGly: 4.91 ± 0.068	1.664ArgHis: 1.664 ± 0.041	3.365ArgIle: 3.365 ± 0.054	1.71ArgLys: 1.71 ± 0.049	7.237ArgLeu: 7.237 ± 0.097	1.68ArgMet: 1.68 ± 0.042	1.401ArgAsn: 1.401 ± 0.036	3.494ArgPro: 3.494 ± 0.068	2.156ArgGln: 2.156 ± 0.044	5.887ArgArg: 5.887 ± 0.095	4.015ArgSer: 4.015 ± 0.063	4.715ArgThr: 4.715 ± 0.067	5.563ArgVal: 5.563 ± 0.072	1.22ArgTrp: 1.22 ± 0.039	1.467ArgTyr: 1.467 ± 0.033	0.001ArgXaa: 0.001 ± 0.001
Ser	6.665SerAla: 6.665 ± 0.083	0.364SerCys: 0.364 ± 0.017	3.432SerAsp: 3.432 ± 0.064	2.678SerGlu: 2.678 ± 0.05	1.821SerPhe: 1.821 ± 0.038	5.781SerGly: 5.781 ± 0.081	1.134SerHis: 1.134 ± 0.032	2.591SerIle: 2.591 ± 0.041	1.434SerLys: 1.434 ± 0.042	5.483SerLeu: 5.483 ± 0.068	1.418SerMet: 1.418 ± 0.032	1.145SerAsn: 1.145 ± 0.036	2.907SerPro: 2.907 ± 0.053	1.47SerGln: 1.47 ± 0.035	3.83SerArg: 3.83 ± 0.054	3.657SerSer: 3.657 ± 0.067	3.822SerThr: 3.822 ± 0.063	4.755SerVal: 4.755 ± 0.063	0.96SerTrp: 0.96 ± 0.033	1.368SerTyr: 1.368 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	7.409ThrAla: 7.409 ± 0.098	0.428ThrCys: 0.428 ± 0.019	3.729ThrAsp: 3.729 ± 0.061	3.006ThrGlu: 3.006 ± 0.058	2.182ThrPhe: 2.182 ± 0.039	5.741ThrGly: 5.741 ± 0.075	1.189ThrHis: 1.189 ± 0.039	3.069ThrIle: 3.069 ± 0.051	1.599ThrLys: 1.599 ± 0.041	6.022ThrLeu: 6.022 ± 0.076	1.24ThrMet: 1.24 ± 0.037	1.279ThrAsn: 1.279 ± 0.031	3.724ThrPro: 3.724 ± 0.062	1.583ThrGln: 1.583 ± 0.04	3.645ThrArg: 3.645 ± 0.058	3.924ThrSer: 3.924 ± 0.068	4.432ThrThr: 4.432 ± 0.074	5.752ThrVal: 5.752 ± 0.078	1.068ThrTrp: 1.068 ± 0.033	1.787ThrTyr: 1.787 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	11.522ValAla: 11.522 ± 0.123	0.644ValCys: 0.644 ± 0.026	6.146ValAsp: 6.146 ± 0.082	5.26ValGlu: 5.26 ± 0.078	2.69ValPhe: 2.69 ± 0.058	7.213ValGly: 7.213 ± 0.085	1.828ValHis: 1.828 ± 0.038	4.184ValIle: 4.184 ± 0.067	2.147ValLys: 2.147 ± 0.051	9.209ValLeu: 9.209 ± 0.111	1.747ValMet: 1.747 ± 0.041	1.922ValAsn: 1.922 ± 0.043	4.649ValPro: 4.649 ± 0.069	2.188ValGln: 2.188 ± 0.042	6.158ValArg: 6.158 ± 0.076	5.039ValSer: 5.039 ± 0.073	6.044ValThr: 6.044 ± 0.075	9.277ValVal: 9.277 ± 0.101	1.144ValTrp: 1.144 ± 0.036	1.433ValTyr: 1.433 ± 0.039	0.002ValXaa: 0.002 ± 0.001
Trp	1.551TrpAla: 1.551 ± 0.032	0.146TrpCys: 0.146 ± 0.01	0.899TrpAsp: 0.899 ± 0.025	0.578TrpGlu: 0.578 ± 0.023	0.575TrpPhe: 0.575 ± 0.024	1.096TrpGly: 1.096 ± 0.038	0.375TrpHis: 0.375 ± 0.019	0.69TrpIle: 0.69 ± 0.026	0.396TrpLys: 0.396 ± 0.02	1.735TrpLeu: 1.735 ± 0.042	0.308TrpMet: 0.308 ± 0.016	0.389TrpAsn: 0.389 ± 0.019	0.748TrpPro: 0.748 ± 0.031	0.577TrpGln: 0.577 ± 0.024	1.18TrpArg: 1.18 ± 0.034	1.007TrpSer: 1.007 ± 0.035	1.047TrpThr: 1.047 ± 0.034	1.132TrpVal: 1.132 ± 0.033	0.374TrpTrp: 0.374 ± 0.018	0.293TrpTyr: 0.293 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.864TyrAla: 2.864 ± 0.055	0.156TyrCys: 0.156 ± 0.012	1.643TyrAsp: 1.643 ± 0.043	1.256TyrGlu: 1.256 ± 0.034	0.716TyrPhe: 0.716 ± 0.026	2.035TyrGly: 2.035 ± 0.044	0.336TyrHis: 0.336 ± 0.017	0.605TyrIle: 0.605 ± 0.024	0.429TyrLys: 0.429 ± 0.021	2.065TyrLeu: 2.065 ± 0.04	0.247TyrMet: 0.247 ± 0.016	0.425TyrAsn: 0.425 ± 0.022	0.966TyrPro: 0.966 ± 0.027	0.545TyrGln: 0.545 ± 0.021	1.503TyrArg: 1.503 ± 0.033	1.075TyrSer: 1.075 ± 0.032	1.06TyrThr: 1.06 ± 0.032	2.153TyrVal: 2.153 ± 0.042	0.338TyrTrp: 0.338 ± 0.017	0.451TyrTyr: 0.451 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.002XaaGly: 0.002 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3738 proteins (1174455 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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