Rhizophagus clarus
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 27461 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z6QKN8|A0A2Z6QKN8_9GLOM Uncharacterized protein OS=Rhizophagus clarus OX=94130 GN=RclHR1_17770002 PE=4 SV=1
MM1 pKa = 7.7 ACVLLDD7 pKa = 4.21 NIEE10 pKa = 5.14 AYY12 pKa = 8.85 PQNPDD17 pKa = 2.65 TLLFVNVTLFEE28 pKa = 5.04 LEE30 pKa = 4.44 DD31 pKa = 4.4 FNGSSQTFTIEE42 pKa = 3.69 PEE44 pKa = 3.68 ICFEE48 pKa = 4.01 VPEE51 pKa = 5.26 DD52 pKa = 3.34 IGHH55 pKa = 6.96 DD56 pKa = 3.44 VGSAIIGDD64 pKa = 3.99 DD65 pKa = 3.67 DD66 pKa = 3.77 CVKK69 pKa = 10.62 FFTGTDD75 pKa = 3.34 CTGNSVCACGEE86 pKa = 4.09 KK87 pKa = 10.45 NFPDD91 pKa = 4.41 GDD93 pKa = 3.98 PFKK96 pKa = 11.42 DD97 pKa = 3.46 NIGSIRR103 pKa = 11.84 LNEE106 pKa = 3.83 VDD108 pKa = 4.32 STIKK112 pKa = 10.65 KK113 pKa = 10.27 CDD115 pKa = 3.28 VV116 pKa = 3.11
Molecular weight: 12.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.719
IPC_protein 3.706
Toseland 3.49
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.401
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 4.05
Thurlkill 3.554
EMBOSS 3.656
Sillero 3.821
Patrickios 0.896
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.741
Protein with the highest isoelectric point:
>tr|A0A2Z6QRH7|A0A2Z6QRH7_9GLOM Uncharacterized protein OS=Rhizophagus clarus OX=94130 GN=RclHR1_20490002 PE=4 SV=1
MM1 pKa = 7.89 DD2 pKa = 4.84 NEE4 pKa = 4.3 EE5 pKa = 3.87 NCINRR10 pKa = 11.84 TIRR13 pKa = 11.84 KK14 pKa = 9.05 RR15 pKa = 11.84 RR16 pKa = 11.84 FKK18 pKa = 10.84 RR19 pKa = 11.84 QLKK22 pKa = 9.02 NKK24 pKa = 9.03 NINVVRR30 pKa = 11.84 RR31 pKa = 11.84 SSFNNPPVINQQRR44 pKa = 11.84 RR45 pKa = 11.84 NPVQRR50 pKa = 11.84 RR51 pKa = 11.84 LPRR54 pKa = 11.84 RR55 pKa = 11.84 RR56 pKa = 11.84 IPPVINQPRR65 pKa = 11.84 RR66 pKa = 11.84 NPVQRR71 pKa = 11.84 RR72 pKa = 11.84 LPRR75 pKa = 11.84 RR76 pKa = 11.84 RR77 pKa = 11.84 RR78 pKa = 3.35
Molecular weight: 9.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.358
IPC2_protein 10.774
IPC_protein 12.369
Toseland 12.53
ProMoST 13.042
Dawson 12.53
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.12
Grimsley 12.574
Solomon 13.027
Lehninger 12.939
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.842
IPC_peptide 13.042
IPC2_peptide 12.018
IPC2.peptide.svr19 9.164
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
27454
7
27461
10447393
12
7141
380.4
43.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.449 ± 0.014
1.634 ± 0.007
5.854 ± 0.011
6.818 ± 0.017
4.592 ± 0.011
4.343 ± 0.012
2.234 ± 0.006
8.165 ± 0.015
8.002 ± 0.018
9.118 ± 0.016
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.901 ± 0.005
7.323 ± 0.018
4.034 ± 0.013
3.836 ± 0.012
4.285 ± 0.011
7.923 ± 0.018
5.294 ± 0.014
4.782 ± 0.011
1.238 ± 0.006
4.158 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here