Klebsiella pneumoniae subsp. rhinoscleromatis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Klebsiella; Klebsiella pneumoniae

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6800 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A378E4B7|A0A378E4B7_KLEPR Maltodextrin glucosidase OS=Klebsiella pneumoniae subsp. rhinoscleromatis OX=39831 GN=NCTC5046_02117 PE=4 SV=1
MM1 pKa = 7.88PNFFIDD7 pKa = 3.58RR8 pKa = 11.84PIFAWVIAIIIMLAGGLSILKK29 pKa = 10.21LPVAQYY35 pKa = 7.81PTIAPPAISITAMYY49 pKa = 9.22PGADD53 pKa = 3.33AEE55 pKa = 4.83TVQNTVTQVIEE66 pKa = 3.98QNMNGIDD73 pKa = 3.44HH74 pKa = 7.12LMYY77 pKa = 10.06MSSNGDD83 pKa = 3.4STGTATITLTFEE95 pKa = 4.26SGTDD99 pKa = 3.31PDD101 pKa = 3.61IAA103 pKa = 5.73

Molecular weight: 11.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A378CPQ3|A0A378CPQ3_KLEPR Delta-aminolevulinic acid dehydratase OS=Klebsiella pneumoniae subsp. rhinoscleromatis OX=39831 GN=hemB_1 PE=3 SV=1
MM1 pKa = 7.31TLFEE5 pKa = 4.63RR6 pKa = 11.84LLHH9 pKa = 6.36RR10 pKa = 11.84RR11 pKa = 11.84LAMSSTLMAAQRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84HH26 pKa = 5.92LNHH29 pKa = 6.71LHH31 pKa = 5.41QAQVLPLWAISLHH44 pKa = 4.66NRR46 pKa = 11.84MVKK49 pKa = 9.1TLPLMVKK56 pKa = 10.4ASKK59 pKa = 10.26KK60 pKa = 8.94RR61 pKa = 11.84LRR63 pKa = 11.84ARR65 pKa = 11.84TEE67 pKa = 3.85SSKK70 pKa = 10.84KK71 pKa = 8.64PRR73 pKa = 11.84KK74 pKa = 9.09SRR76 pKa = 11.84SRR78 pKa = 11.84KK79 pKa = 8.06ARR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84NMSSRR88 pKa = 11.84QVKK91 pKa = 9.87ASSWTLKK98 pKa = 10.49RR99 pKa = 3.64

Molecular weight: 11.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6800

0

6800

1505742

29

1805

221.4

24.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.455 ± 0.037

1.173 ± 0.012

5.06 ± 0.022

5.356 ± 0.028

3.768 ± 0.022

7.624 ± 0.029

2.303 ± 0.017

5.467 ± 0.027

3.842 ± 0.028

10.848 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.819 ± 0.014

3.397 ± 0.023

4.751 ± 0.024

4.525 ± 0.027

6.321 ± 0.032

5.837 ± 0.024

5.194 ± 0.024

6.931 ± 0.025

1.616 ± 0.017

2.712 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski