Volucribacter psittacicida
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2076 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R1FMB6|A0A4R1FMB6_9PAST Phage baseplate assembly protein V OS=Volucribacter psittacicida OX=203482 GN=EV694_1970 PE=4 SV=1
MM1 pKa = 7.65 AIVGLFYY8 pKa = 11.01 GSDD11 pKa = 3.2 TGNTEE16 pKa = 4.47 NIAKK20 pKa = 9.24 MIQKK24 pKa = 9.58 QLGNEE29 pKa = 3.93 LVDD32 pKa = 3.33 IRR34 pKa = 11.84 DD35 pKa = 3.34 IAKK38 pKa = 10.28 SSKK41 pKa = 10.35 EE42 pKa = 3.95 DD43 pKa = 3.02 IEE45 pKa = 5.73 AYY47 pKa = 10.19 DD48 pKa = 4.65 FIMIGIPTWYY58 pKa = 9.99 YY59 pKa = 11.6 GEE61 pKa = 4.69 AQCDD65 pKa = 3.26 WDD67 pKa = 5.24 DD68 pKa = 4.13 FFPTLEE74 pKa = 4.64 QIDD77 pKa = 4.09 FTDD80 pKa = 3.2 KK81 pKa = 10.68 LVAIFGCGDD90 pKa = 3.25 QEE92 pKa = 5.04 DD93 pKa = 4.3 YY94 pKa = 11.79 AEE96 pKa = 4.32 YY97 pKa = 10.5 FCDD100 pKa = 4.34 AMGTVRR106 pKa = 11.84 NIIEE110 pKa = 3.89 PHH112 pKa = 5.31 GAIIVGHH119 pKa = 6.82 WPTEE123 pKa = 4.02 GYY125 pKa = 10.46 NFEE128 pKa = 4.12 VSQALVDD135 pKa = 4.36 DD136 pKa = 4.01 NTFVGLCIDD145 pKa = 4.19 EE146 pKa = 4.82 DD147 pKa = 3.92 RR148 pKa = 11.84 QPEE151 pKa = 4.14 LTAEE155 pKa = 4.22 RR156 pKa = 11.84 VEE158 pKa = 4.21 TWVKK162 pKa = 9.98 QVYY165 pKa = 10.73 DD166 pKa = 4.17 EE167 pKa = 4.42 MCLAEE172 pKa = 4.67 LVNN175 pKa = 4.23
Molecular weight: 19.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.846
IPC_protein 3.834
Toseland 3.63
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.719
Rodwell 3.656
Grimsley 3.528
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.126
Thurlkill 3.668
EMBOSS 3.732
Sillero 3.948
Patrickios 0.807
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.829
Protein with the highest isoelectric point:
>tr|A0A4R1FTK5|A0A4R1FTK5_9PAST KipI family sensor histidine kinase inhibitor OS=Volucribacter psittacicida OX=203482 GN=EV694_1849 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 SRR14 pKa = 11.84 THH16 pKa = 6.4 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.93 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2076
0
2076
661908
29
3581
318.8
35.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.368 ± 0.067
1.04 ± 0.019
4.837 ± 0.05
5.806 ± 0.058
4.261 ± 0.046
6.482 ± 0.063
2.158 ± 0.03
7.307 ± 0.049
5.714 ± 0.048
10.95 ± 0.088
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.311 ± 0.029
4.799 ± 0.067
3.759 ± 0.043
6.288 ± 0.081
4.36 ± 0.052
5.725 ± 0.054
5.094 ± 0.077
6.203 ± 0.05
1.186 ± 0.022
3.354 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here