Luteimicrobium xylanilyticum
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3715 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5P9QBQ2|A0A5P9QBQ2_9MICO Stage 0 sporulation protein OS=Luteimicrobium xylanilyticum OX=1133546 GN=KDY119_02185 PE=4 SV=1
MM1 pKa = 7.38 AAGVAACSPVISVPVATHH19 pKa = 5.93 ATDD22 pKa = 4.02 PACADD27 pKa = 4.1 VILDD31 pKa = 5.07 LPDD34 pKa = 3.19 QVAGQDD40 pKa = 3.38 QRR42 pKa = 11.84 TTSSQATSAWGSAGSAITLRR62 pKa = 11.84 CGVEE66 pKa = 3.89 QPGPSDD72 pKa = 3.14 QCQSIEE78 pKa = 4.23 NPDD81 pKa = 3.53 GTSVDD86 pKa = 3.76 WISAEE91 pKa = 4.19 TSTGWTFVTYY101 pKa = 10.59 GRR103 pKa = 11.84 DD104 pKa = 3.23 PAVEE108 pKa = 4.06 VQVPKK113 pKa = 10.85 SLGEE117 pKa = 4.17 GQPTAALVDD126 pKa = 3.65 VAPAVLDD133 pKa = 3.82 VKK135 pKa = 10.66 ATTTCTGG142 pKa = 3.0
Molecular weight: 14.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.846
IPC_protein 3.808
Toseland 3.592
ProMoST 3.999
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.783
Rodwell 3.643
Grimsley 3.503
Solomon 3.795
Lehninger 3.757
Nozaki 3.935
DTASelect 4.19
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.935
Patrickios 1.901
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.834
Protein with the highest isoelectric point:
>tr|A0A5P9Q748|A0A5P9Q748_9MICO Uncharacterized protein OS=Luteimicrobium xylanilyticum OX=1133546 GN=KDY119_00737 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3715
0
3715
1282968
30
2856
345.3
36.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.104 ± 0.062
0.536 ± 0.009
6.732 ± 0.035
5.033 ± 0.045
2.626 ± 0.023
9.421 ± 0.039
2.126 ± 0.017
2.762 ± 0.033
1.866 ± 0.035
10.006 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.402 ± 0.015
1.601 ± 0.024
5.852 ± 0.033
2.582 ± 0.023
7.706 ± 0.063
5.31 ± 0.039
6.662 ± 0.064
10.175 ± 0.045
1.555 ± 0.017
1.943 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here