Corynebacterium glyciniphilum AJ 3170

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Corynebacteriaceae; Corynebacterium; Corynebacterium glyciniphilum

Average proteome isoelectric point is 5.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3270 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X5DM74|X5DM74_9CORY Thiamine_BP domain-containing protein OS=Corynebacterium glyciniphilum AJ 3170 OX=1404245 GN=CGLY_08865 PE=3 SV=1
MM1 pKa = 7.58KK2 pKa = 9.99IAPKK6 pKa = 9.76TLFATFTVFFLCSACSTEE24 pKa = 4.85GKK26 pKa = 10.49DD27 pKa = 4.23PVDD30 pKa = 3.78PRR32 pKa = 11.84DD33 pKa = 3.52GAEE36 pKa = 4.2VTFSTYY42 pKa = 10.76SYY44 pKa = 11.22DD45 pKa = 3.85DD46 pKa = 3.37EE47 pKa = 4.76TGEE50 pKa = 4.34EE51 pKa = 3.92EE52 pKa = 5.02ALFEE56 pKa = 5.05GKK58 pKa = 9.24LHH60 pKa = 5.94YY61 pKa = 10.49PNDD64 pKa = 3.62GGRR67 pKa = 11.84VYY69 pKa = 11.11GEE71 pKa = 4.26RR72 pKa = 11.84NEE74 pKa = 4.12GTQFGMVFPEE84 pKa = 4.16GTTYY88 pKa = 10.03STDD91 pKa = 3.35SDD93 pKa = 4.23VLKK96 pKa = 11.06SPAQVEE102 pKa = 4.2IPIDD106 pKa = 4.09DD107 pKa = 4.22DD108 pKa = 4.21VSLGGGYY115 pKa = 10.45YY116 pKa = 10.26DD117 pKa = 5.34ASSGDD122 pKa = 3.96DD123 pKa = 3.55NQDD126 pKa = 3.31DD127 pKa = 4.02FDD129 pKa = 4.31EE130 pKa = 4.55YY131 pKa = 11.13FYY133 pKa = 11.76VNSVDD138 pKa = 3.8GEE140 pKa = 4.51SIRR143 pKa = 4.65

Molecular weight: 15.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X5DQZ8|X5DQZ8_9CORY NUDIX domain-containing protein OS=Corynebacterium glyciniphilum AJ 3170 OX=1404245 GN=CGLY_16010 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.63KK15 pKa = 9.39KK16 pKa = 9.04HH17 pKa = 5.39RR18 pKa = 11.84KK19 pKa = 5.37MLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3270

0

3270

1073090

31

3103

328.2

35.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.723 ± 0.05

0.705 ± 0.011

6.856 ± 0.047

5.695 ± 0.05

2.996 ± 0.03

9.144 ± 0.04

2.106 ± 0.021

4.397 ± 0.035

2.108 ± 0.031

9.595 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.164 ± 0.021

2.344 ± 0.024

5.221 ± 0.035

2.833 ± 0.025

6.822 ± 0.048

6.088 ± 0.026

6.737 ± 0.032

8.993 ± 0.04

1.453 ± 0.018

2.021 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski