Nelumbo nucifera (Sacred lotus)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Proteales; Nelumbonaceae; Nelumbo

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31582 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U8B392|A0A1U8B392_NELNU protein EXORDIUM-like 5 OS=Nelumbo nucifera OX=4432 GN=LOC104606814 PE=3 SV=1
MM1 pKa = 8.08VMFYY5 pKa = 9.47VTYY8 pKa = 10.43TEE10 pKa = 4.48AKK12 pKa = 9.41CVCIDD17 pKa = 4.48DD18 pKa = 4.45GCSGKK23 pKa = 10.69GKK25 pKa = 9.72EE26 pKa = 4.02QLQDD30 pKa = 3.67GDD32 pKa = 5.82DD33 pKa = 3.58IDD35 pKa = 5.58CYY37 pKa = 11.43VVDD40 pKa = 4.73DD41 pKa = 4.48EE42 pKa = 5.56GGVVDD47 pKa = 6.29DD48 pKa = 4.08IHH50 pKa = 8.61GNDD53 pKa = 3.59EE54 pKa = 4.4GLDD57 pKa = 3.66GHH59 pKa = 6.96HH60 pKa = 7.29DD61 pKa = 3.49VDD63 pKa = 4.62DD64 pKa = 4.5EE65 pKa = 4.35EE66 pKa = 6.06FEE68 pKa = 4.15GLYY71 pKa = 10.75DD72 pKa = 4.86DD73 pKa = 4.83GCSGKK78 pKa = 10.62GKK80 pKa = 9.77EE81 pKa = 4.79QLQDD85 pKa = 3.84DD86 pKa = 4.31NDD88 pKa = 3.37INYY91 pKa = 9.94YY92 pKa = 10.14VVDD95 pKa = 5.82DD96 pKa = 3.72IHH98 pKa = 7.89GHH100 pKa = 6.68DD101 pKa = 4.18GGLDD105 pKa = 3.45GHH107 pKa = 6.9HH108 pKa = 7.14DD109 pKa = 3.57VDD111 pKa = 4.79DD112 pKa = 5.02GEE114 pKa = 4.8FKK116 pKa = 10.94GLYY119 pKa = 9.79DD120 pKa = 4.14DD121 pKa = 4.4RR122 pKa = 11.84CSGKK126 pKa = 10.39EE127 pKa = 3.85NEE129 pKa = 4.55QLQDD133 pKa = 4.97DD134 pKa = 4.9DD135 pKa = 7.12DD136 pKa = 4.89IDD138 pKa = 5.1CYY140 pKa = 11.53GLDD143 pKa = 3.51SHH145 pKa = 8.14HH146 pKa = 7.31DD147 pKa = 3.44VDD149 pKa = 5.44DD150 pKa = 5.0GEE152 pKa = 5.27FEE154 pKa = 4.24ALYY157 pKa = 10.77DD158 pKa = 4.48DD159 pKa = 4.69GEE161 pKa = 4.35NYY163 pKa = 10.46EE164 pKa = 4.8DD165 pKa = 5.32GNGKK169 pKa = 10.2DD170 pKa = 3.84YY171 pKa = 11.41EE172 pKa = 5.43DD173 pKa = 5.86DD174 pKa = 4.39DD175 pKa = 6.7GEE177 pKa = 4.24DD178 pKa = 4.43HH179 pKa = 7.27EE180 pKa = 5.93GLDD183 pKa = 4.98DD184 pKa = 5.35DD185 pKa = 5.35VGDD188 pKa = 5.02HH189 pKa = 6.73EE190 pKa = 6.16GSDD193 pKa = 4.3DD194 pKa = 4.89DD195 pKa = 6.06GGDD198 pKa = 3.5GGNEE202 pKa = 4.36GIHH205 pKa = 7.0DD206 pKa = 4.27GGGADD211 pKa = 3.87LSEE214 pKa = 4.4YY215 pKa = 11.1DD216 pKa = 5.21PISEE220 pKa = 3.95YY221 pKa = 11.28DD222 pKa = 3.36NPRR225 pKa = 11.84EE226 pKa = 4.0RR227 pKa = 11.84GNTRR231 pKa = 11.84DD232 pKa = 3.48FDD234 pKa = 4.38FSKK237 pKa = 10.53PRR239 pKa = 11.84FRR241 pKa = 11.84VGMEE245 pKa = 4.17FATMKK250 pKa = 10.3EE251 pKa = 4.16FRR253 pKa = 4.28

Molecular weight: 28.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U8Q272|A0A1U8Q272_NELNU uncharacterized protein LOC104590888 OS=Nelumbo nucifera OX=4432 GN=LOC104590888 PE=4 SV=1
MM1 pKa = 7.69GFPIWGNDD9 pKa = 3.11HH10 pKa = 6.45SRR12 pKa = 11.84SHH14 pKa = 6.81SKK16 pKa = 9.93EE17 pKa = 4.01CHH19 pKa = 4.93SHH21 pKa = 5.04SGYY24 pKa = 11.31YY25 pKa = 10.54LFLTKK30 pKa = 10.1IPPSFPTLIAQRR42 pKa = 11.84KK43 pKa = 7.46KK44 pKa = 10.26RR45 pKa = 11.84PFFFGSCPSPSSNQSIQDD63 pKa = 3.31TRR65 pKa = 11.84RR66 pKa = 11.84CRR68 pKa = 11.84TALRR72 pKa = 11.84RR73 pKa = 11.84DD74 pKa = 3.74PPPALIRR81 pKa = 11.84EE82 pKa = 4.37LPLTSSSNQSIQGINEE98 pKa = 3.66VRR100 pKa = 11.84NGRR103 pKa = 11.84QRR105 pKa = 11.84EE106 pKa = 3.94RR107 pKa = 11.84MVRR110 pKa = 11.84NLTWEE115 pKa = 4.32GGISGRR121 pKa = 11.84VADD124 pKa = 4.66DD125 pKa = 3.5DD126 pKa = 3.78QRR128 pKa = 11.84NEE130 pKa = 4.52GIQMSYY136 pKa = 9.74IRR138 pKa = 11.84KK139 pKa = 7.32TKK141 pKa = 10.69LIVSS145 pKa = 4.46

Molecular weight: 16.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23993

7589

31582

15121372

29

7123

478.8

53.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.611 ± 0.012

1.901 ± 0.007

5.278 ± 0.008

6.645 ± 0.025

4.096 ± 0.009

6.593 ± 0.013

2.398 ± 0.006

5.339 ± 0.009

5.971 ± 0.012

9.781 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.411 ± 0.005

4.342 ± 0.008

4.925 ± 0.014

3.826 ± 0.01

5.419 ± 0.011

9.167 ± 0.019

4.867 ± 0.01

6.439 ± 0.009

1.262 ± 0.005

2.715 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski