Brevibacterium luteolum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Brevibacteriaceae; Brevibacterium

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2715 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N6PIE3|A0A2N6PIE3_9MICO Class I SAM-dependent methyltransferase OS=Brevibacterium luteolum OX=199591 GN=CJ198_03665 PE=4 SV=1
MM1 pKa = 7.56HH2 pKa = 7.53PFSHH6 pKa = 7.35PALALIAAGAALTLAAGCTAAEE28 pKa = 4.49DD29 pKa = 4.47APGSSPAPSTPEE41 pKa = 3.77PPQLSEE47 pKa = 5.57AITLDD52 pKa = 4.48FYY54 pKa = 11.97ASLLADD60 pKa = 3.82NDD62 pKa = 3.41TDD64 pKa = 3.75RR65 pKa = 11.84ALVADD70 pKa = 4.06AMGGRR75 pKa = 11.84LVAVGADD82 pKa = 3.75GAEE85 pKa = 4.29LWTHH89 pKa = 5.37QAQVDD94 pKa = 3.74MDD96 pKa = 4.24RR97 pKa = 11.84PDD99 pKa = 3.33SVAAYY104 pKa = 9.91SHH106 pKa = 6.88GEE108 pKa = 4.05TVIVDD113 pKa = 3.79DD114 pKa = 3.78GTGRR118 pKa = 11.84LRR120 pKa = 11.84ALDD123 pKa = 3.41WGDD126 pKa = 3.75GSEE129 pKa = 3.86AWTYY133 pKa = 10.29TPADD137 pKa = 3.76ADD139 pKa = 4.3LGCTEE144 pKa = 4.64PGDD147 pKa = 4.34YY148 pKa = 10.5YY149 pKa = 11.25SSSSTGTSALGEE161 pKa = 4.27DD162 pKa = 4.72GIILLEE168 pKa = 4.06YY169 pKa = 11.16GMIDD173 pKa = 3.75ADD175 pKa = 4.33RR176 pKa = 11.84GSCTGDD182 pKa = 3.12EE183 pKa = 4.25QKK185 pKa = 11.74AMVLGLDD192 pKa = 3.91PASGQPAWPAVQATADD208 pKa = 3.77AQPMGGANLQITPDD222 pKa = 3.41RR223 pKa = 11.84THH225 pKa = 7.39AYY227 pKa = 10.15LPWRR231 pKa = 11.84DD232 pKa = 3.16GDD234 pKa = 3.98GSFLTRR240 pKa = 11.84LDD242 pKa = 3.85LASGQHH248 pKa = 5.1ATVDD252 pKa = 3.36LSEE255 pKa = 4.8YY256 pKa = 10.92GEE258 pKa = 3.94QDD260 pKa = 3.3LGFGIYY266 pKa = 8.45PQKK269 pKa = 11.16APGTLVYY276 pKa = 10.89AFGTADD282 pKa = 3.55PDD284 pKa = 3.53APYY287 pKa = 10.29SGSVTRR293 pKa = 11.84TALLRR298 pKa = 11.84LPMDD302 pKa = 4.14LPEE305 pKa = 5.04SSEE308 pKa = 3.98ALTPLSAADD317 pKa = 4.44DD318 pKa = 4.46PGLAAGIDD326 pKa = 4.15FSEE329 pKa = 4.9EE330 pKa = 3.83PVDD333 pKa = 3.63YY334 pKa = 10.84CAARR338 pKa = 11.84PVSTASGTFVCASTQLFASRR358 pKa = 11.84ILVLGSLLDD367 pKa = 3.72GEE369 pKa = 5.07AWHH372 pKa = 6.82TDD374 pKa = 2.5ISEE377 pKa = 4.12DD378 pKa = 3.36AFEE381 pKa = 4.95FGLFNEE387 pKa = 4.76QYY389 pKa = 10.88GRR391 pKa = 11.84LHH393 pKa = 6.19TPVDD397 pKa = 3.74GTDD400 pKa = 3.32APLVIMPGLDD410 pKa = 2.8AGVRR414 pKa = 11.84AVSAVDD420 pKa = 5.48GSTLWEE426 pKa = 4.49AGEE429 pKa = 4.87DD430 pKa = 4.12LDD432 pKa = 5.44PLDD435 pKa = 4.7PVEE438 pKa = 4.24GSYY441 pKa = 11.58GFGGNGAFPQLGLIPVLLNDD461 pKa = 3.64AVHH464 pKa = 7.02FFTLDD469 pKa = 3.44GKK471 pKa = 9.95EE472 pKa = 3.98HH473 pKa = 6.86AEE475 pKa = 3.76AHH477 pKa = 6.22AVGQYY482 pKa = 10.7AEE484 pKa = 4.54PSATGRR490 pKa = 11.84WFAAAAGEE498 pKa = 4.52STTLWTVTGGG508 pKa = 3.29

Molecular weight: 52.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N6PGI4|A0A2N6PGI4_9MICO NUDIX hydrolase OS=Brevibacterium luteolum OX=199591 GN=CJ198_09325 PE=4 SV=1
MM1 pKa = 7.56SKK3 pKa = 8.12HH4 pKa = 5.4TFQPNNRR11 pKa = 11.84KK12 pKa = 9.39RR13 pKa = 11.84SKK15 pKa = 10.21KK16 pKa = 9.28HH17 pKa = 4.28GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AVLAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.16GRR40 pKa = 11.84KK41 pKa = 8.86ALSAA45 pKa = 3.99

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2715

0

2715

903331

32

2678

332.7

35.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.069 ± 0.073

0.72 ± 0.011

6.262 ± 0.046

6.181 ± 0.045

3.104 ± 0.027

8.704 ± 0.042

2.192 ± 0.022

4.938 ± 0.034

2.488 ± 0.038

9.639 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.07 ± 0.019

2.092 ± 0.026

5.339 ± 0.032

3.173 ± 0.028

6.756 ± 0.046

5.74 ± 0.033

6.333 ± 0.037

7.87 ± 0.037

1.319 ± 0.02

2.011 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski