Amino acid dipepetide frequency for Brevibacterium luteolum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.646AlaAla: 20.646 ± 0.231	1.016AlaCys: 1.016 ± 0.042	8.682AlaAsp: 8.682 ± 0.115	9.846AlaGlu: 9.846 ± 0.136	3.671AlaPhe: 3.671 ± 0.078	13.067AlaGly: 13.067 ± 0.14	2.758AlaHis: 2.758 ± 0.055	5.501AlaIle: 5.501 ± 0.093	3.193AlaLys: 3.193 ± 0.077	12.244AlaLeu: 12.244 ± 0.149	2.6AlaMet: 2.6 ± 0.054	2.227AlaAsn: 2.227 ± 0.049	5.647AlaPro: 5.647 ± 0.078	4.177AlaGln: 4.177 ± 0.08	8.005AlaArg: 8.005 ± 0.123	6.582AlaSer: 6.582 ± 0.09	6.691AlaThr: 6.691 ± 0.091	10.181AlaVal: 10.181 ± 0.114	1.626AlaTrp: 1.626 ± 0.047	2.326AlaTyr: 2.326 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	0.951CysAla: 0.951 ± 0.032	0.073CysCys: 0.073 ± 0.01	0.427CysAsp: 0.427 ± 0.023	0.425CysGlu: 0.425 ± 0.022	0.234CysPhe: 0.234 ± 0.016	0.807CysGly: 0.807 ± 0.037	0.138CysHis: 0.138 ± 0.012	0.281CysIle: 0.281 ± 0.017	0.117CysLys: 0.117 ± 0.012	0.614CysLeu: 0.614 ± 0.026	0.121CysMet: 0.121 ± 0.009	0.138CysAsn: 0.138 ± 0.012	0.422CysPro: 0.422 ± 0.023	0.226CysGln: 0.226 ± 0.017	0.528CysArg: 0.528 ± 0.026	0.423CysSer: 0.423 ± 0.021	0.505CysThr: 0.505 ± 0.023	0.526CysVal: 0.526 ± 0.028	0.104CysTrp: 0.104 ± 0.011	0.139CysTyr: 0.139 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	7.91AspAla: 7.91 ± 0.105	0.404AspCys: 0.404 ± 0.023	4.444AspAsp: 4.444 ± 0.116	4.762AspGlu: 4.762 ± 0.091	2.045AspPhe: 2.045 ± 0.051	5.434AspGly: 5.434 ± 0.105	1.31AspHis: 1.31 ± 0.039	2.95AspIle: 2.95 ± 0.066	1.333AspLys: 1.333 ± 0.05	5.663AspLeu: 5.663 ± 0.091	1.119AspMet: 1.119 ± 0.032	1.144AspAsn: 1.144 ± 0.038	4.103AspPro: 4.103 ± 0.071	1.662AspGln: 1.662 ± 0.042	4.126AspArg: 4.126 ± 0.08	3.223AspSer: 3.223 ± 0.067	3.57AspThr: 3.57 ± 0.068	5.028AspVal: 5.028 ± 0.078	0.925AspTrp: 0.925 ± 0.033	1.468AspTyr: 1.468 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	8.257GluAla: 8.257 ± 0.124	0.354GluCys: 0.354 ± 0.021	3.593GluAsp: 3.593 ± 0.076	3.737GluGlu: 3.737 ± 0.064	1.843GluPhe: 1.843 ± 0.047	3.761GluGly: 3.761 ± 0.069	1.773GluHis: 1.773 ± 0.047	3.106GluIle: 3.106 ± 0.061	1.967GluLys: 1.967 ± 0.062	6.78GluLeu: 6.78 ± 0.097	1.104GluMet: 1.104 ± 0.037	1.452GluAsn: 1.452 ± 0.045	3.18GluPro: 3.18 ± 0.076	2.554GluGln: 2.554 ± 0.058	4.556GluArg: 4.556 ± 0.074	3.132GluSer: 3.132 ± 0.05	3.612GluThr: 3.612 ± 0.064	5.14GluVal: 5.14 ± 0.077	0.707GluTrp: 0.707 ± 0.03	1.203GluTyr: 1.203 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	4.046PheAla: 4.046 ± 0.074	0.247PheCys: 0.247 ± 0.017	2.548PheAsp: 2.548 ± 0.051	1.707PheGlu: 1.707 ± 0.05	1.129PhePhe: 1.129 ± 0.038	3.276PheGly: 3.276 ± 0.066	0.596PheHis: 0.596 ± 0.025	1.491PheIle: 1.491 ± 0.048	0.607PheLys: 0.607 ± 0.027	2.732PheLeu: 2.732 ± 0.06	0.54PheMet: 0.54 ± 0.025	0.747PheAsn: 0.747 ± 0.029	1.364PhePro: 1.364 ± 0.042	0.68PheGln: 0.68 ± 0.031	1.553PheArg: 1.553 ± 0.042	1.998PheSer: 1.998 ± 0.052	2.237PheThr: 2.237 ± 0.047	2.462PheVal: 2.462 ± 0.05	0.42PheTrp: 0.42 ± 0.024	0.658PheTyr: 0.658 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	9.448GlyAla: 9.448 ± 0.116	0.728GlyCys: 0.728 ± 0.028	4.819GlyAsp: 4.819 ± 0.094	5.19GlyGlu: 5.19 ± 0.079	3.198GlyPhe: 3.198 ± 0.064	7.079GlyGly: 7.079 ± 0.129	1.964GlyHis: 1.964 ± 0.047	4.914GlyIle: 4.914 ± 0.086	2.516GlyLys: 2.516 ± 0.063	8.603GlyLeu: 8.603 ± 0.112	2.187GlyMet: 2.187 ± 0.051	1.757GlyAsn: 1.757 ± 0.049	3.904GlyPro: 3.904 ± 0.077	3.186GlyGln: 3.186 ± 0.066	5.807GlyArg: 5.807 ± 0.096	5.626GlySer: 5.626 ± 0.086	5.784GlyThr: 5.784 ± 0.1	6.666GlyVal: 6.666 ± 0.093	1.469GlyTrp: 1.469 ± 0.041	2.197GlyTyr: 2.197 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	2.678HisAla: 2.678 ± 0.066	0.161HisCys: 0.161 ± 0.015	1.355HisAsp: 1.355 ± 0.048	1.22HisGlu: 1.22 ± 0.033	0.611HisPhe: 0.611 ± 0.026	1.922HisGly: 1.922 ± 0.057	0.6HisHis: 0.6 ± 0.029	1.001HisIle: 1.001 ± 0.031	0.42HisLys: 0.42 ± 0.021	2.161HisLeu: 2.161 ± 0.051	0.383HisMet: 0.383 ± 0.019	0.433HisAsn: 0.433 ± 0.019	1.641HisPro: 1.641 ± 0.046	0.664HisGln: 0.664 ± 0.031	1.773HisArg: 1.773 ± 0.047	1.181HisSer: 1.181 ± 0.036	1.361HisThr: 1.361 ± 0.039	1.582HisVal: 1.582 ± 0.047	0.275HisTrp: 0.275 ± 0.017	0.505HisTyr: 0.505 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	6.907IleAla: 6.907 ± 0.103	0.439IleCys: 0.439 ± 0.02	3.931IleAsp: 3.931 ± 0.073	3.039IleGlu: 3.039 ± 0.059	1.399IlePhe: 1.399 ± 0.046	5.037IleGly: 5.037 ± 0.092	0.896IleHis: 0.896 ± 0.032	2.608IleIle: 2.608 ± 0.076	0.938IleLys: 0.938 ± 0.035	3.761IleLeu: 3.761 ± 0.076	0.891IleMet: 0.891 ± 0.03	1.093IleAsn: 1.093 ± 0.033	2.424IlePro: 2.424 ± 0.049	0.986IleGln: 0.986 ± 0.036	2.893IleArg: 2.893 ± 0.059	2.926IleSer: 2.926 ± 0.06	3.427IleThr: 3.427 ± 0.074	4.442IleVal: 4.442 ± 0.089	0.476IleTrp: 0.476 ± 0.023	0.865IleTyr: 0.865 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	3.054LysAla: 3.054 ± 0.068	0.113LysCys: 0.113 ± 0.012	1.572LysAsp: 1.572 ± 0.054	1.355LysGlu: 1.355 ± 0.045	0.596LysPhe: 0.596 ± 0.027	1.688LysGly: 1.688 ± 0.051	0.633LysHis: 0.633 ± 0.026	1.203LysIle: 1.203 ± 0.044	1.188LysLys: 1.188 ± 0.062	2.332LysLeu: 2.332 ± 0.056	0.517LysMet: 0.517 ± 0.025	0.716LysAsn: 0.716 ± 0.029	1.489LysPro: 1.489 ± 0.047	0.959LysGln: 0.959 ± 0.032	1.893LysArg: 1.893 ± 0.054	1.344LysSer: 1.344 ± 0.045	1.628LysThr: 1.628 ± 0.051	1.813LysVal: 1.813 ± 0.058	0.259LysTrp: 0.259 ± 0.017	0.525LysTyr: 0.525 ± 0.029	0.0LysXaa: 0.0 ± 0.0
Leu	13.255LeuAla: 13.255 ± 0.167	0.643LeuCys: 0.643 ± 0.025	6.226LeuAsp: 6.226 ± 0.093	4.728LeuGlu: 4.728 ± 0.073	2.734LeuPhe: 2.734 ± 0.065	8.416LeuGly: 8.416 ± 0.116	1.879LeuHis: 1.879 ± 0.046	5.301LeuIle: 5.301 ± 0.091	2.284LeuLys: 2.284 ± 0.055	9.338LeuLeu: 9.338 ± 0.151	2.026LeuMet: 2.026 ± 0.05	2.077LeuAsn: 2.077 ± 0.046	5.454LeuPro: 5.454 ± 0.078	2.431LeuGln: 2.431 ± 0.053	6.703LeuArg: 6.703 ± 0.107	5.955LeuSer: 5.955 ± 0.094	6.98LeuThr: 6.98 ± 0.094	7.323LeuVal: 7.323 ± 0.114	1.068LeuTrp: 1.068 ± 0.037	1.573LeuTyr: 1.573 ± 0.05	0.0LeuXaa: 0.0 ± 0.0
Met	2.43MetAla: 2.43 ± 0.055	0.159MetCys: 0.159 ± 0.013	0.984MetAsp: 0.984 ± 0.03	0.823MetGlu: 0.823 ± 0.03	0.648MetPhe: 0.648 ± 0.029	1.438MetGly: 1.438 ± 0.043	0.494MetHis: 0.494 ± 0.02	0.949MetIle: 0.949 ± 0.034	0.57MetLys: 0.57 ± 0.028	2.204MetLeu: 2.204 ± 0.054	0.4MetMet: 0.4 ± 0.022	0.591MetAsn: 0.591 ± 0.026	1.26MetPro: 1.26 ± 0.041	0.607MetGln: 0.607 ± 0.024	1.597MetArg: 1.597 ± 0.04	1.676MetSer: 1.676 ± 0.047	1.865MetThr: 1.865 ± 0.046	1.408MetVal: 1.408 ± 0.037	0.246MetTrp: 0.246 ± 0.015	0.35MetTyr: 0.35 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.513AsnAla: 2.513 ± 0.058	0.144AsnCys: 0.144 ± 0.013	1.175AsnAsp: 1.175 ± 0.031	1.113AsnGlu: 1.113 ± 0.027	0.619AsnPhe: 0.619 ± 0.027	1.825AsnGly: 1.825 ± 0.059	0.435AsnHis: 0.435 ± 0.023	1.018AsnIle: 1.018 ± 0.031	0.478AsnLys: 0.478 ± 0.023	1.996AsnLeu: 1.996 ± 0.045	0.403AsnMet: 0.403 ± 0.023	0.492AsnAsn: 0.492 ± 0.032	1.699AsnPro: 1.699 ± 0.048	0.606AsnGln: 0.606 ± 0.026	1.645AsnArg: 1.645 ± 0.048	1.104AsnSer: 1.104 ± 0.033	1.335AsnThr: 1.335 ± 0.053	1.553AsnVal: 1.553 ± 0.044	0.303AsnTrp: 0.303 ± 0.019	0.468AsnTyr: 0.468 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	7.699ProAla: 7.699 ± 0.115	0.237ProCys: 0.237 ± 0.013	3.666ProAsp: 3.666 ± 0.067	4.384ProGlu: 4.384 ± 0.067	1.47ProPhe: 1.47 ± 0.042	5.349ProGly: 5.349 ± 0.086	1.232ProHis: 1.232 ± 0.035	2.047ProIle: 2.047 ± 0.054	1.292ProLys: 1.292 ± 0.039	4.377ProLeu: 4.377 ± 0.075	1.031ProMet: 1.031 ± 0.036	1.066ProAsn: 1.066 ± 0.03	2.352ProPro: 2.352 ± 0.075	1.945ProGln: 1.945 ± 0.041	2.931ProArg: 2.931 ± 0.063	2.848ProSer: 2.848 ± 0.062	3.031ProThr: 3.031 ± 0.067	4.749ProVal: 4.749 ± 0.082	0.664ProTrp: 0.664 ± 0.031	1.016ProTyr: 1.016 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	4.355GlnAla: 4.355 ± 0.075	0.164GlnCys: 0.164 ± 0.015	1.311GlnAsp: 1.311 ± 0.037	1.533GlnGlu: 1.533 ± 0.039	0.87GlnPhe: 0.87 ± 0.031	1.861GlnGly: 1.861 ± 0.046	0.811GlnHis: 0.811 ± 0.031	1.597GlnIle: 1.597 ± 0.041	0.862GlnLys: 0.862 ± 0.034	3.773GlnLeu: 3.773 ± 0.07	0.758GlnMet: 0.758 ± 0.029	0.649GlnAsn: 0.649 ± 0.026	2.131GlnPro: 2.131 ± 0.065	1.594GlnGln: 1.594 ± 0.053	2.523GlnArg: 2.523 ± 0.06	1.534GlnSer: 1.534 ± 0.037	1.797GlnThr: 1.797 ± 0.048	2.549GlnVal: 2.549 ± 0.056	0.474GlnTrp: 0.474 ± 0.022	0.588GlnTyr: 0.588 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	7.478ArgAla: 7.478 ± 0.096	0.508ArgCys: 0.508 ± 0.024	3.656ArgAsp: 3.656 ± 0.076	4.283ArgGlu: 4.283 ± 0.077	2.228ArgPhe: 2.228 ± 0.045	4.798ArgGly: 4.798 ± 0.078	1.492ArgHis: 1.492 ± 0.036	3.762ArgIle: 3.762 ± 0.068	1.796ArgLys: 1.796 ± 0.054	6.854ArgLeu: 6.854 ± 0.103	1.782ArgMet: 1.782 ± 0.042	1.458ArgAsn: 1.458 ± 0.041	3.365ArgPro: 3.365 ± 0.072	2.541ArgGln: 2.541 ± 0.058	5.853ArgArg: 5.853 ± 0.11	4.117ArgSer: 4.117 ± 0.079	4.419ArgThr: 4.419 ± 0.083	4.628ArgVal: 4.628 ± 0.077	1.008ArgTrp: 1.008 ± 0.033	1.529ArgTyr: 1.529 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	7.25SerAla: 7.25 ± 0.091	0.392SerCys: 0.392 ± 0.021	3.37SerAsp: 3.37 ± 0.066	3.331SerGlu: 3.331 ± 0.067	1.862SerPhe: 1.862 ± 0.041	6.07SerGly: 6.07 ± 0.097	1.177SerHis: 1.177 ± 0.039	2.531SerIle: 2.531 ± 0.055	1.364SerLys: 1.364 ± 0.041	5.233SerLeu: 5.233 ± 0.071	1.425SerMet: 1.425 ± 0.038	1.139SerAsn: 1.139 ± 0.04	3.157SerPro: 3.157 ± 0.065	1.908SerGln: 1.908 ± 0.046	3.86SerArg: 3.86 ± 0.081	3.35SerSer: 3.35 ± 0.064	3.599SerThr: 3.599 ± 0.067	4.274SerVal: 4.274 ± 0.067	0.835SerTrp: 0.835 ± 0.032	1.273SerTyr: 1.273 ± 0.039	0.0SerXaa: 0.0 ± 0.0
Thr	8.727ThrAla: 8.727 ± 0.11	0.431ThrCys: 0.431 ± 0.027	3.868ThrAsp: 3.868 ± 0.073	3.711ThrGlu: 3.711 ± 0.062	1.78ThrPhe: 1.78 ± 0.053	6.764ThrGly: 6.764 ± 0.09	1.295ThrHis: 1.295 ± 0.04	3.072ThrIle: 3.072 ± 0.064	1.313ThrLys: 1.313 ± 0.038	5.532ThrLeu: 5.532 ± 0.087	1.157ThrMet: 1.157 ± 0.039	1.183ThrAsn: 1.183 ± 0.042	3.739ThrPro: 3.739 ± 0.069	1.744ThrGln: 1.744 ± 0.046	3.815ThrArg: 3.815 ± 0.071	3.639ThrSer: 3.639 ± 0.078	3.958ThrThr: 3.958 ± 0.085	5.502ThrVal: 5.502 ± 0.084	0.84ThrTrp: 0.84 ± 0.032	1.258ThrTyr: 1.258 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	9.31ValAla: 9.31 ± 0.12	0.692ValCys: 0.692 ± 0.03	4.974ValAsp: 4.974 ± 0.087	4.766ValGlu: 4.766 ± 0.083	2.803ValPhe: 2.803 ± 0.064	5.662ValGly: 5.662 ± 0.084	1.717ValHis: 1.717 ± 0.046	4.592ValIle: 4.592 ± 0.075	1.86ValLys: 1.86 ± 0.058	8.482ValLeu: 8.482 ± 0.117	1.592ValMet: 1.592 ± 0.042	1.751ValAsn: 1.751 ± 0.045	4.369ValPro: 4.369 ± 0.069	1.961ValGln: 1.961 ± 0.046	4.992ValArg: 4.992 ± 0.077	4.778ValSer: 4.778 ± 0.072	5.544ValThr: 5.544 ± 0.096	6.484ValVal: 6.484 ± 0.091	0.938ValTrp: 0.938 ± 0.032	1.434ValTyr: 1.434 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.426TrpAla: 1.426 ± 0.04	0.11TrpCys: 0.11 ± 0.012	0.662TrpAsp: 0.662 ± 0.034	0.674TrpGlu: 0.674 ± 0.028	0.557TrpPhe: 0.557 ± 0.028	0.835TrpGly: 0.835 ± 0.034	0.333TrpHis: 0.333 ± 0.019	0.69TrpIle: 0.69 ± 0.035	0.379TrpLys: 0.379 ± 0.019	1.645TrpLeu: 1.645 ± 0.053	0.349TrpMet: 0.349 ± 0.021	0.369TrpAsn: 0.369 ± 0.022	0.679TrpPro: 0.679 ± 0.028	0.658TrpGln: 0.658 ± 0.03	0.881TrpArg: 0.881 ± 0.031	0.753TrpSer: 0.753 ± 0.035	0.744TrpThr: 0.744 ± 0.026	0.922TrpVal: 0.922 ± 0.033	0.272TrpTrp: 0.272 ± 0.017	0.255TrpTyr: 0.255 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.347TyrAla: 2.347 ± 0.051	0.185TyrCys: 0.185 ± 0.014	1.358TyrAsp: 1.358 ± 0.044	1.189TyrGlu: 1.189 ± 0.036	0.741TyrPhe: 0.741 ± 0.026	1.956TyrGly: 1.956 ± 0.052	0.392TyrHis: 0.392 ± 0.019	0.763TyrIle: 0.763 ± 0.031	0.403TyrLys: 0.403 ± 0.022	2.07TyrLeu: 2.07 ± 0.05	0.312TyrMet: 0.312 ± 0.02	0.442TyrAsn: 0.442 ± 0.022	1.007TyrPro: 1.007 ± 0.033	0.649TyrGln: 0.649 ± 0.032	1.621TyrArg: 1.621 ± 0.046	1.211TyrSer: 1.211 ± 0.04	1.239TyrThr: 1.239 ± 0.046	1.465TyrVal: 1.465 ± 0.039	0.28TyrTrp: 0.28 ± 0.019	0.482TyrTyr: 0.482 ± 0.028	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2715 proteins (903331 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---