Glomus cerebriforme
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 20972 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A397TL31|A0A397TL31_9GLOM Trafficking protein particle complex subunit OS=Glomus cerebriforme OX=658196 GN=C1645_718793 PE=3 SV=1
MM1 pKa = 7.92 PILVFEE7 pKa = 4.56 WNEE10 pKa = 3.75 GGFNDD15 pKa = 4.94 VPDD18 pKa = 4.3 APGLRR23 pKa = 11.84 NGVAGQTKK31 pKa = 9.83 AAIVANLMANGATNYY46 pKa = 10.42 NDD48 pKa = 3.69 IIFAFSSGHH57 pKa = 7.5 AIGEE61 pKa = 4.1 WCRR64 pKa = 11.84 QISMNIQWALNQPGVPNICNSITRR88 pKa = 11.84 INPIIRR94 pKa = 11.84 YY95 pKa = 9.38 EE96 pKa = 4.95 DD97 pKa = 4.73 DD98 pKa = 5.86 DD99 pKa = 7.25 DD100 pKa = 7.26 DD101 pKa = 6.98 DD102 pKa = 6.69 DD103 pKa = 4.1 EE104 pKa = 4.8 TGIPSPEE111 pKa = 3.66 FDD113 pKa = 3.46 IEE115 pKa = 4.1 NYY117 pKa = 9.69 PAFGYY122 pKa = 10.51 CC123 pKa = 4.49
Molecular weight: 13.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.808
IPC_protein 3.757
Toseland 3.554
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.592
Grimsley 3.465
Solomon 3.745
Lehninger 3.694
Nozaki 3.884
DTASelect 4.113
Thurlkill 3.617
EMBOSS 3.719
Sillero 3.884
Patrickios 0.846
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.792
Protein with the highest isoelectric point:
>tr|A0A397S1M7|A0A397S1M7_9GLOM Uncharacterized protein (Fragment) OS=Glomus cerebriforme OX=658196 GN=C1645_794415 PE=4 SV=1
MM1 pKa = 7.76 IILLTSTHH9 pKa = 5.68 LTSIRR14 pKa = 11.84 VQITVKK20 pKa = 8.81 NHH22 pKa = 4.47 RR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 KK26 pKa = 9.09 RR27 pKa = 11.84 WITSWTRR34 pKa = 11.84 RR35 pKa = 11.84 IRR37 pKa = 11.84 KK38 pKa = 9.33 SLVIVLGNAIKK49 pKa = 10.58 SRR51 pKa = 11.84 NLKK54 pKa = 9.98 KK55 pKa = 10.28 RR56 pKa = 11.84 SKK58 pKa = 10.78 HH59 pKa = 5.62 LLTKK63 pKa = 10.27 IKK65 pKa = 10.8 NLISGG70 pKa = 4.03
Molecular weight: 8.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.515
IPC2_protein 11.228
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.749
Grimsley 13.013
Solomon 13.466
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.486
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.134
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
20968
4
20972
6808095
49
5062
324.6
37.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.507 ± 0.017
1.531 ± 0.009
5.722 ± 0.013
6.951 ± 0.019
4.69 ± 0.014
4.542 ± 0.018
2.152 ± 0.008
8.25 ± 0.018
7.896 ± 0.021
9.187 ± 0.019
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.007 ± 0.006
7.089 ± 0.02
4.06 ± 0.015
3.824 ± 0.013
4.264 ± 0.013
7.884 ± 0.018
5.296 ± 0.012
4.971 ± 0.016
1.158 ± 0.006
3.981 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here