Halobellus sp. Atlit-38R
Average proteome isoelectric point is 4.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3864 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A498G955|A0A498G955_9EURY Phosphonate ABC transporter permease protein PhnE OS=Halobellus sp. Atlit-38R OX=2282131 GN=phnE PE=3 SV=1
MM1 pKa = 7.7 SDD3 pKa = 2.91 SHH5 pKa = 8.44 DD6 pKa = 3.66 NDD8 pKa = 3.27 GLTRR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 ALGAGGAVLTLALAGCSGTGDD35 pKa = 3.25 GGTTEE40 pKa = 4.55 TEE42 pKa = 4.47 TATSTQTDD50 pKa = 3.82 TATPTAEE57 pKa = 4.09 PTTTEE62 pKa = 3.81 TGEE65 pKa = 4.09 ASLSALEE72 pKa = 4.24 VPEE75 pKa = 4.69 DD76 pKa = 4.04 ANCAVCNMKK85 pKa = 9.58 PAKK88 pKa = 10.34 FPDD91 pKa = 3.7 YY92 pKa = 10.54 NAQLQYY98 pKa = 10.93 EE99 pKa = 4.25 SSEE102 pKa = 4.05 PKK104 pKa = 10.12 FFCSNGCMASFAADD118 pKa = 3.5 PGHH121 pKa = 6.63 FEE123 pKa = 3.98 EE124 pKa = 6.39 AYY126 pKa = 10.54 ADD128 pKa = 3.74 ASIAAAFVHH137 pKa = 7.0 DD138 pKa = 5.24 HH139 pKa = 7.11 DD140 pKa = 4.18 TTDD143 pKa = 3.77 WIDD146 pKa = 3.62 GLAASYY152 pKa = 10.35 VLEE155 pKa = 4.3 MDD157 pKa = 4.11 SEE159 pKa = 4.45 RR160 pKa = 11.84 VDD162 pKa = 3.34 DD163 pKa = 4.3 PMRR166 pKa = 11.84 INPLAFDD173 pKa = 4.16 TEE175 pKa = 4.39 ADD177 pKa = 3.01 ATAYY181 pKa = 10.25 VEE183 pKa = 4.71 QYY185 pKa = 11.69 DD186 pKa = 4.28 DD187 pKa = 3.76 LTAEE191 pKa = 4.38 DD192 pKa = 4.3 VVGFDD197 pKa = 4.89 AFDD200 pKa = 4.51 RR201 pKa = 11.84 DD202 pKa = 3.24 LAEE205 pKa = 3.95 QYY207 pKa = 9.95 RR208 pKa = 11.84 ASFFEE213 pKa = 4.12
Molecular weight: 22.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.77
IPC_protein 3.77
Toseland 3.567
ProMoST 3.923
Dawson 3.77
Bjellqvist 3.948
Wikipedia 3.694
Rodwell 3.605
Grimsley 3.465
Solomon 3.757
Lehninger 3.706
Nozaki 3.872
DTASelect 4.101
Thurlkill 3.605
EMBOSS 3.706
Sillero 3.897
Patrickios 1.163
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A498GFM9|A0A498GFM9_9EURY TIGR03663 family protein OS=Halobellus sp. Atlit-38R OX=2282131 GN=D3D02_02410 PE=4 SV=1
MM1 pKa = 7.52 GPRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 AGRR9 pKa = 11.84 GVPPRR14 pKa = 11.84 ARR16 pKa = 11.84 PRR18 pKa = 11.84 RR19 pKa = 11.84 ARR21 pKa = 11.84 GRR23 pKa = 11.84 RR24 pKa = 11.84 PRR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 TRR31 pKa = 11.84 GPTTTRR37 pKa = 11.84 SGPARR42 pKa = 11.84 TAPRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 TRR50 pKa = 11.84 RR51 pKa = 11.84 RR52 pKa = 11.84 HH53 pKa = 4.52 RR54 pKa = 11.84 RR55 pKa = 11.84 CRR57 pKa = 11.84 RR58 pKa = 11.84 RR59 pKa = 11.84 RR60 pKa = 11.84 RR61 pKa = 11.84 PSEE64 pKa = 3.74 RR65 pKa = 11.84 RR66 pKa = 3.31
Molecular weight: 8.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.228
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.501
Grimsley 13.042
Solomon 13.495
Lehninger 13.393
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.208
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.219
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3864
0
3864
1075213
21
3681
278.3
30.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.95 ± 0.054
0.657 ± 0.013
8.273 ± 0.049
8.384 ± 0.058
3.317 ± 0.03
8.42 ± 0.041
1.943 ± 0.022
4.179 ± 0.034
1.913 ± 0.024
8.88 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.667 ± 0.02
2.425 ± 0.027
4.559 ± 0.025
2.755 ± 0.036
6.451 ± 0.05
5.809 ± 0.036
6.551 ± 0.058
9.07 ± 0.046
1.102 ± 0.017
2.697 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here