Amino acid dipepetide frequency for Halobellus sp. Atlit-38R

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.077AlaAla: 14.077 ± 0.173
0.618AlaCys: 0.618 ± 0.029
9.618AlaAsp: 9.618 ± 0.109
8.769AlaGlu: 8.769 ± 0.103
3.843AlaPhe: 3.843 ± 0.075
9.49AlaGly: 9.49 ± 0.102
1.815AlaHis: 1.815 ± 0.042
4.869AlaIle: 4.869 ± 0.078
1.958AlaLys: 1.958 ± 0.048
10.082AlaLeu: 10.082 ± 0.137
1.941AlaMet: 1.941 ± 0.042
2.506AlaAsn: 2.506 ± 0.056
4.024AlaPro: 4.024 ± 0.071
2.431AlaGln: 2.431 ± 0.051
6.145AlaArg: 6.145 ± 0.095
5.91AlaSer: 5.91 ± 0.091
7.178AlaThr: 7.178 ± 0.105
10.36AlaVal: 10.36 ± 0.101
1.118AlaTrp: 1.118 ± 0.035
2.746AlaTyr: 2.746 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.546CysAla: 0.546 ± 0.023
0.075CysCys: 0.075 ± 0.008
0.521CysAsp: 0.521 ± 0.024
0.543CysGlu: 0.543 ± 0.024
0.17CysPhe: 0.17 ± 0.013
0.798CysGly: 0.798 ± 0.029
0.176CysHis: 0.176 ± 0.012
0.233CysIle: 0.233 ± 0.015
0.119CysLys: 0.119 ± 0.012
0.502CysLeu: 0.502 ± 0.021
0.104CysMet: 0.104 ± 0.009
0.176CysAsn: 0.176 ± 0.013
0.485CysPro: 0.485 ± 0.022
0.159CysGln: 0.159 ± 0.012
0.442CysArg: 0.442 ± 0.021
0.407CysSer: 0.407 ± 0.019
0.384CysThr: 0.384 ± 0.021
0.47CysVal: 0.47 ± 0.023
0.078CysTrp: 0.078 ± 0.009
0.177CysTyr: 0.177 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
10.708AspAla: 10.708 ± 0.126
0.544AspCys: 0.544 ± 0.023
7.665AspAsp: 7.665 ± 0.12
7.728AspGlu: 7.728 ± 0.105
2.148AspPhe: 2.148 ± 0.046
7.625AspGly: 7.625 ± 0.104
1.58AspHis: 1.58 ± 0.04
3.043AspIle: 3.043 ± 0.055
1.03AspLys: 1.03 ± 0.039
6.894AspLeu: 6.894 ± 0.083
0.998AspMet: 0.998 ± 0.03
1.322AspAsn: 1.322 ± 0.046
4.502AspPro: 4.502 ± 0.077
1.843AspGln: 1.843 ± 0.063
5.926AspArg: 5.926 ± 0.091
3.881AspSer: 3.881 ± 0.068
4.158AspThr: 4.158 ± 0.067
8.483AspVal: 8.483 ± 0.101
0.85AspTrp: 0.85 ± 0.03
1.804AspTyr: 1.804 ± 0.04
0.0AspXaa: 0.0 ± 0.0
Glu
8.451GluAla: 8.451 ± 0.111
0.531GluCys: 0.531 ± 0.026
5.518GluAsp: 5.518 ± 0.088
6.615GluGlu: 6.615 ± 0.098
2.835GluPhe: 2.835 ± 0.06
5.038GluGly: 5.038 ± 0.08
2.028GluHis: 2.028 ± 0.042
3.993GluIle: 3.993 ± 0.065
1.884GluLys: 1.884 ± 0.051
7.346GluLeu: 7.346 ± 0.086
2.015GluMet: 2.015 ± 0.048
2.446GluAsn: 2.446 ± 0.053
3.512GluPro: 3.512 ± 0.063
3.02GluGln: 3.02 ± 0.07
7.271GluArg: 7.271 ± 0.104
5.451GluSer: 5.451 ± 0.077
6.559GluThr: 6.559 ± 0.093
5.51GluVal: 5.51 ± 0.082
1.126GluTrp: 1.126 ± 0.037
2.687GluTyr: 2.687 ± 0.056
0.0GluXaa: 0.0 ± 0.0
Phe
3.666PheAla: 3.666 ± 0.082
0.268PheCys: 0.268 ± 0.016
3.106PheAsp: 3.106 ± 0.055
3.304PheGlu: 3.304 ± 0.066
1.089PhePhe: 1.089 ± 0.037
3.206PheGly: 3.206 ± 0.064
0.617PheHis: 0.617 ± 0.026
1.085PheIle: 1.085 ± 0.037
0.49PheLys: 0.49 ± 0.022
2.957PheLeu: 2.957 ± 0.067
0.419PheMet: 0.419 ± 0.02
0.715PheAsn: 0.715 ± 0.03
1.377PhePro: 1.377 ± 0.037
0.913PheGln: 0.913 ± 0.028
1.783PheArg: 1.783 ± 0.042
1.795PheSer: 1.795 ± 0.041
1.876PheThr: 1.876 ± 0.046
3.29PheVal: 3.29 ± 0.072
0.378PheTrp: 0.378 ± 0.019
0.835PheTyr: 0.835 ± 0.031
0.0PheXaa: 0.0 ± 0.0
Gly
8.171GlyAla: 8.171 ± 0.105
0.64GlyCys: 0.64 ± 0.027
6.627GlyAsp: 6.627 ± 0.092
6.774GlyGlu: 6.774 ± 0.087
3.088GlyPhe: 3.088 ± 0.064
7.484GlyGly: 7.484 ± 0.123
1.642GlyHis: 1.642 ± 0.047
4.208GlyIle: 4.208 ± 0.077
1.784GlyLys: 1.784 ± 0.041
7.255GlyLeu: 7.255 ± 0.094
1.586GlyMet: 1.586 ± 0.046
1.948GlyAsn: 1.948 ± 0.049
3.308GlyPro: 3.308 ± 0.061
2.151GlyGln: 2.151 ± 0.05
4.87GlyArg: 4.87 ± 0.077
5.377GlySer: 5.377 ± 0.085
5.914GlyThr: 5.914 ± 0.09
7.713GlyVal: 7.713 ± 0.094
1.002GlyTrp: 1.002 ± 0.034
2.658GlyTyr: 2.658 ± 0.057
0.0GlyXaa: 0.0 ± 0.0
His
1.946HisAla: 1.946 ± 0.039
0.18HisCys: 0.18 ± 0.014
1.658HisAsp: 1.658 ± 0.042
1.69HisGlu: 1.69 ± 0.041
0.565HisPhe: 0.565 ± 0.024
1.831HisGly: 1.831 ± 0.052
0.484HisHis: 0.484 ± 0.025
0.777HisIle: 0.777 ± 0.031
0.308HisLys: 0.308 ± 0.017
1.716HisLeu: 1.716 ± 0.045
0.234HisMet: 0.234 ± 0.016
0.469HisAsn: 0.469 ± 0.02
1.184HisPro: 1.184 ± 0.032
0.481HisGln: 0.481 ± 0.022
1.295HisArg: 1.295 ± 0.035
0.905HisSer: 0.905 ± 0.033
1.107HisThr: 1.107 ± 0.033
1.796HisVal: 1.796 ± 0.042
0.23HisTrp: 0.23 ± 0.015
0.574HisTyr: 0.574 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
4.65IleAla: 4.65 ± 0.072
0.252IleCys: 0.252 ± 0.015
3.929IleAsp: 3.929 ± 0.063
4.394IleGlu: 4.394 ± 0.07
1.116IlePhe: 1.116 ± 0.03
3.817IleGly: 3.817 ± 0.067
0.839IleHis: 0.839 ± 0.029
1.427IleIle: 1.427 ± 0.041
0.787IleLys: 0.787 ± 0.027
3.218IleLeu: 3.218 ± 0.066
0.433IleMet: 0.433 ± 0.021
0.987IleAsn: 0.987 ± 0.032
2.13IlePro: 2.13 ± 0.052
1.202IleGln: 1.202 ± 0.04
2.692IleArg: 2.692 ± 0.052
2.321IleSer: 2.321 ± 0.057
2.495IleThr: 2.495 ± 0.06
3.796IleVal: 3.796 ± 0.065
0.337IleTrp: 0.337 ± 0.018
0.964IleTyr: 0.964 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
1.777LysAla: 1.777 ± 0.044
0.139LysCys: 0.139 ± 0.011
1.06LysAsp: 1.06 ± 0.037
1.403LysGlu: 1.403 ± 0.047
0.578LysPhe: 0.578 ± 0.027
1.317LysGly: 1.317 ± 0.039
0.492LysHis: 0.492 ± 0.026
0.885LysIle: 0.885 ± 0.035
0.582LysLys: 0.582 ± 0.029
1.698LysLeu: 1.698 ± 0.042
0.369LysMet: 0.369 ± 0.016
0.62LysAsn: 0.62 ± 0.026
0.893LysPro: 0.893 ± 0.029
0.872LysGln: 0.872 ± 0.034
1.652LysArg: 1.652 ± 0.041
1.27LysSer: 1.27 ± 0.038
1.411LysThr: 1.411 ± 0.043
1.202LysVal: 1.202 ± 0.035
0.277LysTrp: 0.277 ± 0.016
0.634LysTyr: 0.634 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
10.476LeuAla: 10.476 ± 0.129
0.55LeuCys: 0.55 ± 0.021
7.66LeuAsp: 7.66 ± 0.102
6.333LeuGlu: 6.333 ± 0.081
3.196LeuPhe: 3.196 ± 0.072
7.786LeuGly: 7.786 ± 0.116
1.492LeuHis: 1.492 ± 0.043
2.928LeuIle: 2.928 ± 0.063
1.679LeuLys: 1.679 ± 0.039
8.608LeuLeu: 8.608 ± 0.136
1.226LeuMet: 1.226 ± 0.038
1.921LeuAsn: 1.921 ± 0.042
4.085LeuPro: 4.085 ± 0.07
2.282LeuGln: 2.282 ± 0.051
5.607LeuArg: 5.607 ± 0.077
5.958LeuSer: 5.958 ± 0.08
5.531LeuThr: 5.531 ± 0.075
8.404LeuVal: 8.404 ± 0.109
0.875LeuTrp: 0.875 ± 0.029
2.202LeuTyr: 2.202 ± 0.05
0.0LeuXaa: 0.0 ± 0.0
Met
1.664MetAla: 1.664 ± 0.04
0.122MetCys: 0.122 ± 0.011
1.197MetAsp: 1.197 ± 0.035
1.037MetGlu: 1.037 ± 0.035
0.459MetPhe: 0.459 ± 0.021
1.29MetGly: 1.29 ± 0.037
0.317MetHis: 0.317 ± 0.017
0.689MetIle: 0.689 ± 0.026
0.428MetLys: 0.428 ± 0.019
1.533MetLeu: 1.533 ± 0.046
0.309MetMet: 0.309 ± 0.017
0.561MetAsn: 0.561 ± 0.024
0.777MetPro: 0.777 ± 0.027
0.557MetGln: 0.557 ± 0.022
1.095MetArg: 1.095 ± 0.031
1.529MetSer: 1.529 ± 0.037
1.451MetThr: 1.451 ± 0.037
1.152MetVal: 1.152 ± 0.03
0.134MetTrp: 0.134 ± 0.012
0.376MetTyr: 0.376 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
2.745AsnAla: 2.745 ± 0.053
0.208AsnCys: 0.208 ± 0.016
1.818AsnAsp: 1.818 ± 0.051
1.814AsnGlu: 1.814 ± 0.048
0.727AsnPhe: 0.727 ± 0.027
2.069AsnGly: 2.069 ± 0.054
0.472AsnHis: 0.472 ± 0.021
1.033AsnIle: 1.033 ± 0.031
0.489AsnLys: 0.489 ± 0.022
2.104AsnLeu: 2.104 ± 0.046
0.369AsnMet: 0.369 ± 0.019
0.593AsnAsn: 0.593 ± 0.031
1.519AsnPro: 1.519 ± 0.037
0.691AsnGln: 0.691 ± 0.028
1.629AsnArg: 1.629 ± 0.041
1.127AsnSer: 1.127 ± 0.037
1.456AsnThr: 1.456 ± 0.048
2.386AsnVal: 2.386 ± 0.054
0.317AsnTrp: 0.317 ± 0.019
0.682AsnTyr: 0.682 ± 0.025
0.0AsnXaa: 0.0 ± 0.0
Pro
4.554ProAla: 4.554 ± 0.068
0.214ProCys: 0.214 ± 0.014
4.467ProAsp: 4.467 ± 0.08
4.567ProGlu: 4.567 ± 0.067
1.591ProPhe: 1.591 ± 0.046
3.616ProGly: 3.616 ± 0.071
0.914ProHis: 0.914 ± 0.028
2.065ProIle: 2.065 ± 0.045
0.901ProLys: 0.901 ± 0.031
3.672ProLeu: 3.672 ± 0.062
0.829ProMet: 0.829 ± 0.028
1.218ProAsn: 1.218 ± 0.034
2.048ProPro: 2.048 ± 0.048
1.121ProGln: 1.121 ± 0.034
2.253ProArg: 2.253 ± 0.05
2.679ProSer: 2.679 ± 0.054
3.298ProThr: 3.298 ± 0.064
3.951ProVal: 3.951 ± 0.071
0.518ProTrp: 0.518 ± 0.023
1.116ProTyr: 1.116 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
2.567GlnAla: 2.567 ± 0.056
0.189GlnCys: 0.189 ± 0.013
1.481GlnAsp: 1.481 ± 0.075
1.925GlnGlu: 1.925 ± 0.057
1.197GlnPhe: 1.197 ± 0.03
1.7GlnGly: 1.7 ± 0.043
0.587GlnHis: 0.587 ± 0.024
1.358GlnIle: 1.358 ± 0.04
0.684GlnLys: 0.684 ± 0.024
2.544GlnLeu: 2.544 ± 0.054
0.7GlnMet: 0.7 ± 0.024
0.87GlnAsn: 0.87 ± 0.034
1.14GlnPro: 1.14 ± 0.036
1.169GlnGln: 1.169 ± 0.043
2.152GlnArg: 2.152 ± 0.048
2.028GlnSer: 2.028 ± 0.061
2.073GlnThr: 2.073 ± 0.054
1.861GlnVal: 1.861 ± 0.043
0.366GlnTrp: 0.366 ± 0.021
0.957GlnTyr: 0.957 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
6.385ArgAla: 6.385 ± 0.09
0.415ArgCys: 0.415 ± 0.022
5.053ArgAsp: 5.053 ± 0.082
6.353ArgGlu: 6.353 ± 0.09
2.138ArgPhe: 2.138 ± 0.046
4.555ArgGly: 4.555 ± 0.072
1.234ArgHis: 1.234 ± 0.037
3.078ArgIle: 3.078 ± 0.058
1.428ArgLys: 1.428 ± 0.039
5.939ArgLeu: 5.939 ± 0.083
1.217ArgMet: 1.217 ± 0.037
1.639ArgAsn: 1.639 ± 0.041
2.599ArgPro: 2.599 ± 0.057
2.003ArgGln: 2.003 ± 0.045
4.947ArgArg: 4.947 ± 0.092
3.685ArgSer: 3.685 ± 0.066
3.943ArgThr: 3.943 ± 0.073
5.266ArgVal: 5.266 ± 0.08
0.781ArgTrp: 0.781 ± 0.031
1.846ArgTyr: 1.846 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
5.808SerAla: 5.808 ± 0.09
0.317SerCys: 0.317 ± 0.018
4.641SerAsp: 4.641 ± 0.076
4.828SerGlu: 4.828 ± 0.08
1.939SerPhe: 1.939 ± 0.044
5.744SerGly: 5.744 ± 0.086
1.095SerHis: 1.095 ± 0.029
2.617SerIle: 2.617 ± 0.064
1.285SerLys: 1.285 ± 0.039
5.147SerLeu: 5.147 ± 0.073
1.053SerMet: 1.053 ± 0.033
1.546SerAsn: 1.546 ± 0.037
2.663SerPro: 2.663 ± 0.055
1.686SerGln: 1.686 ± 0.065
3.238SerArg: 3.238 ± 0.054
3.252SerSer: 3.252 ± 0.071
3.849SerThr: 3.849 ± 0.072
5.306SerVal: 5.306 ± 0.096
0.625SerTrp: 0.625 ± 0.025
1.454SerTyr: 1.454 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
7.239ThrAla: 7.239 ± 0.098
0.326ThrCys: 0.326 ± 0.017
5.431ThrAsp: 5.431 ± 0.08
4.737ThrGlu: 4.737 ± 0.064
2.174ThrPhe: 2.174 ± 0.051
5.828ThrGly: 5.828 ± 0.082
1.279ThrHis: 1.279 ± 0.034
2.971ThrIle: 2.971 ± 0.065
1.107ThrLys: 1.107 ± 0.037
5.797ThrLeu: 5.797 ± 0.08
1.032ThrMet: 1.032 ± 0.034
1.642ThrAsn: 1.642 ± 0.046
3.482ThrPro: 3.482 ± 0.066
1.655ThrGln: 1.655 ± 0.056
3.419ThrArg: 3.419 ± 0.069
3.319ThrSer: 3.319 ± 0.065
4.567ThrThr: 4.567 ± 0.113
6.956ThrVal: 6.956 ± 0.128
0.66ThrTrp: 0.66 ± 0.026
1.889ThrTyr: 1.889 ± 0.046
0.0ThrXaa: 0.0 ± 0.0
Val
10.387ValAla: 10.387 ± 0.138
0.665ValCys: 0.665 ± 0.026
7.922ValAsp: 7.922 ± 0.094
7.721ValGlu: 7.721 ± 0.084
3.016ValPhe: 3.016 ± 0.06
7.866ValGly: 7.866 ± 0.112
1.536ValHis: 1.536 ± 0.034
3.19ValIle: 3.19 ± 0.049
1.43ValLys: 1.43 ± 0.036
8.008ValLeu: 8.008 ± 0.104
1.283ValMet: 1.283 ± 0.037
2.042ValAsn: 2.042 ± 0.048
4.158ValPro: 4.158 ± 0.07
2.049ValGln: 2.049 ± 0.043
5.357ValArg: 5.357 ± 0.074
5.346ValSer: 5.346 ± 0.083
6.084ValThr: 6.084 ± 0.118
9.619ValVal: 9.619 ± 0.124
0.859ValTrp: 0.859 ± 0.031
2.157ValTyr: 2.157 ± 0.045
0.0ValXaa: 0.0 ± 0.0
Trp
0.898TrpAla: 0.898 ± 0.031
0.096TrpCys: 0.096 ± 0.01
0.776TrpAsp: 0.776 ± 0.029
0.851TrpGlu: 0.851 ± 0.032
0.429TrpPhe: 0.429 ± 0.024
0.815TrpGly: 0.815 ± 0.03
0.219TrpHis: 0.219 ± 0.015
0.523TrpIle: 0.523 ± 0.023
0.274TrpLys: 0.274 ± 0.015
1.153TrpLeu: 1.153 ± 0.034
0.207TrpMet: 0.207 ± 0.015
0.376TrpAsn: 0.376 ± 0.019
0.418TrpPro: 0.418 ± 0.02
0.399TrpGln: 0.399 ± 0.02
0.829TrpArg: 0.829 ± 0.029
0.61TrpSer: 0.61 ± 0.028
0.773TrpThr: 0.773 ± 0.028
0.815TrpVal: 0.815 ± 0.035
0.18TrpTrp: 0.18 ± 0.013
0.383TrpTyr: 0.383 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.782TyrAla: 2.782 ± 0.052
0.218TyrCys: 0.218 ± 0.013
2.586TyrAsp: 2.586 ± 0.054
2.452TyrGlu: 2.452 ± 0.054
0.871TyrPhe: 0.871 ± 0.029
2.323TyrGly: 2.323 ± 0.05
0.612TyrHis: 0.612 ± 0.026
0.813TyrIle: 0.813 ± 0.029
0.485TyrLys: 0.485 ± 0.025
2.626TyrLeu: 2.626 ± 0.051
0.349TyrMet: 0.349 ± 0.018
0.654TyrAsn: 0.654 ± 0.024
1.286TyrPro: 1.286 ± 0.032
0.861TyrGln: 0.861 ± 0.03
1.906TyrArg: 1.906 ± 0.041
1.242TyrSer: 1.242 ± 0.036
1.401TyrThr: 1.401 ± 0.043
2.363TyrVal: 2.363 ± 0.05
0.312TyrTrp: 0.312 ± 0.016
0.827TyrTyr: 0.827 ± 0.03
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3864 proteins (1075213 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski