Aeromonas phage PS1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 247 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514TUZ4|A0A514TUZ4_9CAUD Uncharacterized protein OS=Aeromonas phage PS1 OX=2591406 GN=PS1_0088 PE=4 SV=1
MM1 pKa = 7.27NAINQTLPVEE11 pKa = 4.15PLTIFTNPAPVMFKK25 pKa = 10.94LNSCDD30 pKa = 3.41LSDD33 pKa = 3.7YY34 pKa = 11.13FEE36 pKa = 5.6GYY38 pKa = 10.31NSEE41 pKa = 3.97QTLIALEE48 pKa = 4.26GALNIIFNPDD58 pKa = 2.59ILLARR63 pKa = 11.84VDD65 pKa = 3.81DD66 pKa = 4.11TLTTVEE72 pKa = 4.22DD73 pKa = 4.19LGWDD77 pKa = 3.47SVEE80 pKa = 3.93NTITSEE86 pKa = 4.46TVFKK90 pKa = 10.82LVNQHH95 pKa = 6.96DD96 pKa = 5.02SYY98 pKa = 11.78TAMTHH103 pKa = 6.49EE104 pKa = 4.66EE105 pKa = 4.1LTDD108 pKa = 3.44AFANSFDD115 pKa = 4.52SIVNKK120 pKa = 10.33LSEE123 pKa = 5.81DD124 pKa = 2.99ITGDD128 pKa = 3.36HH129 pKa = 6.77FNSDD133 pKa = 2.68VSTIYY138 pKa = 10.95SEE140 pKa = 3.89MGTFNSITVNSYY152 pKa = 10.84LYY154 pKa = 9.99EE155 pKa = 4.03EE156 pKa = 4.66RR157 pKa = 11.84SNVLRR162 pKa = 11.84VYY164 pKa = 9.99LTFNMYY170 pKa = 10.49

Molecular weight: 19.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514TUV8|A0A514TUV8_9CAUD Uncharacterized protein OS=Aeromonas phage PS1 OX=2591406 GN=PS1_0048 PE=4 SV=1
MM1 pKa = 7.53GKK3 pKa = 10.12AIYY6 pKa = 10.03LSYY9 pKa = 10.95PFILTLHH16 pKa = 6.69FYY18 pKa = 10.3VKK20 pKa = 10.47SILYY24 pKa = 10.07VIGLLFLIFEE34 pKa = 4.46RR35 pKa = 11.84HH36 pKa = 4.93LTWFLHH42 pKa = 5.71SFLNRR47 pKa = 11.84IYY49 pKa = 10.66FRR51 pKa = 11.84FKK53 pKa = 10.88GGLGGPGQSGVQRR66 pKa = 11.84SSAGPITT73 pKa = 3.7

Molecular weight: 8.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

247

0

247

73213

36

2347

296.4

33.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.229 ± 0.196

1.054 ± 0.049

6.164 ± 0.081

6.721 ± 0.149

4.834 ± 0.079

6.339 ± 0.166

1.916 ± 0.069

6.764 ± 0.139

7.193 ± 0.144

8.953 ± 0.129

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.408 ± 0.08

6.116 ± 0.108

3.723 ± 0.104

3.434 ± 0.089

4.207 ± 0.091

6.603 ± 0.113

5.929 ± 0.101

7.193 ± 0.114

1.061 ± 0.042

4.158 ± 0.096
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski