Amino acid dipepetide frequency for Aeromonas phage PS1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.715AlaAla: 3.715 ± 0.342
0.683AlaCys: 0.683 ± 0.107
2.978AlaAsp: 2.978 ± 0.238
3.196AlaGlu: 3.196 ± 0.27
2.172AlaPhe: 2.172 ± 0.175
3.947AlaGly: 3.947 ± 0.32
0.751AlaHis: 0.751 ± 0.107
3.51AlaIle: 3.51 ± 0.21
3.797AlaLys: 3.797 ± 0.325
5.122AlaLeu: 5.122 ± 0.286
1.68AlaMet: 1.68 ± 0.176
2.978AlaAsn: 2.978 ± 0.238
1.571AlaPro: 1.571 ± 0.171
1.94AlaGln: 1.94 ± 0.191
2.213AlaArg: 2.213 ± 0.17
3.114AlaSer: 3.114 ± 0.288
2.704AlaThr: 2.704 ± 0.224
3.756AlaVal: 3.756 ± 0.22
0.478AlaTrp: 0.478 ± 0.074
1.981AlaTyr: 1.981 ± 0.173
0.0AlaXaa: 0.0 ± 0.0
Cys
0.3CysAla: 0.3 ± 0.067
0.123CysCys: 0.123 ± 0.047
0.574CysAsp: 0.574 ± 0.099
0.724CysGlu: 0.724 ± 0.097
0.574CysPhe: 0.574 ± 0.092
0.601CysGly: 0.601 ± 0.101
0.205CysHis: 0.205 ± 0.053
0.505CysIle: 0.505 ± 0.074
1.079CysLys: 1.079 ± 0.147
0.929CysLeu: 0.929 ± 0.109
0.232CysMet: 0.232 ± 0.052
0.669CysAsn: 0.669 ± 0.1
0.314CysPro: 0.314 ± 0.072
0.396CysGln: 0.396 ± 0.073
0.519CysArg: 0.519 ± 0.094
0.765CysSer: 0.765 ± 0.092
0.533CysThr: 0.533 ± 0.094
0.861CysVal: 0.861 ± 0.129
0.123CysTrp: 0.123 ± 0.047
0.519CysTyr: 0.519 ± 0.08
0.0CysXaa: 0.0 ± 0.0
Asp
2.582AspAla: 2.582 ± 0.203
0.738AspCys: 0.738 ± 0.104
3.169AspAsp: 3.169 ± 0.25
3.729AspGlu: 3.729 ± 0.277
3.579AspPhe: 3.579 ± 0.22
3.784AspGly: 3.784 ± 0.256
1.229AspHis: 1.229 ± 0.128
4.603AspIle: 4.603 ± 0.229
4.822AspLys: 4.822 ± 0.319
6.133AspLeu: 6.133 ± 0.239
1.489AspMet: 1.489 ± 0.157
3.825AspAsn: 3.825 ± 0.257
2.896AspPro: 2.896 ± 0.212
2.199AspGln: 2.199 ± 0.164
2.445AspArg: 2.445 ± 0.172
3.442AspSer: 3.442 ± 0.25
2.95AspThr: 2.95 ± 0.198
4.139AspVal: 4.139 ± 0.185
0.806AspTrp: 0.806 ± 0.104
3.087AspTyr: 3.087 ± 0.21
0.0AspXaa: 0.0 ± 0.0
Glu
3.579GluAla: 3.579 ± 0.271
0.519GluCys: 0.519 ± 0.082
3.674GluAsp: 3.674 ± 0.186
5.067GluGlu: 5.067 ± 0.353
3.456GluPhe: 3.456 ± 0.24
3.551GluGly: 3.551 ± 0.252
1.68GluHis: 1.68 ± 0.15
4.767GluIle: 4.767 ± 0.234
4.152GluLys: 4.152 ± 0.35
7.253GluLeu: 7.253 ± 0.366
1.707GluMet: 1.707 ± 0.15
3.579GluAsn: 3.579 ± 0.171
1.953GluPro: 1.953 ± 0.166
3.019GluGln: 3.019 ± 0.224
3.073GluArg: 3.073 ± 0.233
3.988GluSer: 3.988 ± 0.214
3.947GluThr: 3.947 ± 0.227
4.671GluVal: 4.671 ± 0.296
0.779GluTrp: 0.779 ± 0.112
2.8GluTyr: 2.8 ± 0.205
0.0GluXaa: 0.0 ± 0.0
Phe
2.049PheAla: 2.049 ± 0.194
0.738PheCys: 0.738 ± 0.124
3.551PheAsp: 3.551 ± 0.233
2.677PheGlu: 2.677 ± 0.21
2.09PhePhe: 2.09 ± 0.154
3.469PheGly: 3.469 ± 0.199
0.806PheHis: 0.806 ± 0.104
3.551PheIle: 3.551 ± 0.274
4.029PheLys: 4.029 ± 0.219
3.251PheLeu: 3.251 ± 0.198
1.12PheMet: 1.12 ± 0.115
3.893PheAsn: 3.893 ± 0.224
1.571PhePro: 1.571 ± 0.163
1.106PheGln: 1.106 ± 0.127
1.735PheArg: 1.735 ± 0.174
3.456PheSer: 3.456 ± 0.232
3.36PheThr: 3.36 ± 0.223
3.346PheVal: 3.346 ± 0.212
0.56PheTrp: 0.56 ± 0.075
1.981PheTyr: 1.981 ± 0.148
0.0PheXaa: 0.0 ± 0.0
Gly
2.636GlyAla: 2.636 ± 0.267
0.71GlyCys: 0.71 ± 0.102
3.729GlyAsp: 3.729 ± 0.258
4.016GlyGlu: 4.016 ± 0.259
3.442GlyPhe: 3.442 ± 0.206
4.166GlyGly: 4.166 ± 0.403
0.861GlyHis: 0.861 ± 0.114
4.712GlyIle: 4.712 ± 0.248
5.108GlyLys: 5.108 ± 0.327
6.024GlyLeu: 6.024 ± 0.304
1.653GlyMet: 1.653 ± 0.17
3.702GlyAsn: 3.702 ± 0.274
1.216GlyPro: 1.216 ± 0.151
1.994GlyGln: 1.994 ± 0.154
2.677GlyArg: 2.677 ± 0.201
4.303GlySer: 4.303 ± 0.247
3.674GlyThr: 3.674 ± 0.21
4.753GlyVal: 4.753 ± 0.285
0.956GlyTrp: 0.956 ± 0.113
3.06GlyTyr: 3.06 ± 0.211
0.0GlyXaa: 0.0 ± 0.0
His
0.929HisAla: 0.929 ± 0.12
0.15HisCys: 0.15 ± 0.047
1.024HisAsp: 1.024 ± 0.129
0.983HisGlu: 0.983 ± 0.112
1.216HisPhe: 1.216 ± 0.121
1.038HisGly: 1.038 ± 0.122
0.437HisHis: 0.437 ± 0.072
1.216HisIle: 1.216 ± 0.144
1.311HisLys: 1.311 ± 0.139
1.967HisLeu: 1.967 ± 0.154
0.287HisMet: 0.287 ± 0.067
0.983HisAsn: 0.983 ± 0.107
1.202HisPro: 1.202 ± 0.131
0.806HisGln: 0.806 ± 0.118
1.243HisArg: 1.243 ± 0.153
1.106HisSer: 1.106 ± 0.134
0.915HisThr: 0.915 ± 0.118
1.161HisVal: 1.161 ± 0.144
0.3HisTrp: 0.3 ± 0.056
0.888HisTyr: 0.888 ± 0.118
0.0HisXaa: 0.0 ± 0.0
Ile
3.469IleAla: 3.469 ± 0.211
0.451IleCys: 0.451 ± 0.067
5.286IleAsp: 5.286 ± 0.267
4.385IleGlu: 4.385 ± 0.268
2.65IlePhe: 2.65 ± 0.203
3.92IleGly: 3.92 ± 0.237
1.27IleHis: 1.27 ± 0.148
3.947IleIle: 3.947 ± 0.262
5.955IleLys: 5.955 ± 0.347
4.712IleLeu: 4.712 ± 0.302
1.339IleMet: 1.339 ± 0.118
4.986IleAsn: 4.986 ± 0.313
3.428IlePro: 3.428 ± 0.212
2.144IleGln: 2.144 ± 0.16
2.923IleArg: 2.923 ± 0.166
5.081IleSer: 5.081 ± 0.299
4.617IleThr: 4.617 ± 0.22
3.865IleVal: 3.865 ± 0.217
0.492IleTrp: 0.492 ± 0.102
2.718IleTyr: 2.718 ± 0.19
0.0IleXaa: 0.0 ± 0.0
Lys
4.412LysAla: 4.412 ± 0.256
0.601LysCys: 0.601 ± 0.094
4.794LysAsp: 4.794 ± 0.264
6.898LysGlu: 6.898 ± 0.369
3.114LysPhe: 3.114 ± 0.178
4.207LysGly: 4.207 ± 0.268
1.721LysHis: 1.721 ± 0.168
3.865LysIle: 3.865 ± 0.239
3.62LysLys: 3.62 ± 0.258
7.594LysLeu: 7.594 ± 0.36
1.666LysMet: 1.666 ± 0.168
3.36LysAsn: 3.36 ± 0.233
2.827LysPro: 2.827 ± 0.192
2.827LysGln: 2.827 ± 0.221
3.36LysArg: 3.36 ± 0.202
4.125LysSer: 4.125 ± 0.252
3.715LysThr: 3.715 ± 0.207
5.573LysVal: 5.573 ± 0.285
0.779LysTrp: 0.779 ± 0.11
2.855LysTyr: 2.855 ± 0.206
0.0LysXaa: 0.0 ± 0.0
Leu
4.89LeuAla: 4.89 ± 0.306
0.929LeuCys: 0.929 ± 0.115
6.01LeuAsp: 6.01 ± 0.26
6.392LeuGlu: 6.392 ± 0.302
4.344LeuPhe: 4.344 ± 0.229
5.505LeuGly: 5.505 ± 0.325
1.543LeuHis: 1.543 ± 0.157
6.106LeuIle: 6.106 ± 0.354
6.406LeuLys: 6.406 ± 0.3
7.089LeuLeu: 7.089 ± 0.361
2.022LeuMet: 2.022 ± 0.174
5.901LeuAsn: 5.901 ± 0.233
3.401LeuPro: 3.401 ± 0.208
2.827LeuGln: 2.827 ± 0.187
3.756LeuArg: 3.756 ± 0.225
6.283LeuSer: 6.283 ± 0.25
5.983LeuThr: 5.983 ± 0.321
6.379LeuVal: 6.379 ± 0.39
0.587LeuTrp: 0.587 ± 0.089
3.183LeuTyr: 3.183 ± 0.2
0.0LeuXaa: 0.0 ± 0.0
Met
1.161MetAla: 1.161 ± 0.129
0.191MetCys: 0.191 ± 0.054
1.653MetAsp: 1.653 ± 0.149
1.694MetGlu: 1.694 ± 0.15
1.366MetPhe: 1.366 ± 0.126
1.421MetGly: 1.421 ± 0.133
0.341MetHis: 0.341 ± 0.075
1.543MetIle: 1.543 ± 0.154
1.967MetLys: 1.967 ± 0.169
2.103MetLeu: 2.103 ± 0.196
0.642MetMet: 0.642 ± 0.082
1.639MetAsn: 1.639 ± 0.145
0.683MetPro: 0.683 ± 0.118
0.656MetGln: 0.656 ± 0.096
0.792MetArg: 0.792 ± 0.136
2.103MetSer: 2.103 ± 0.179
1.53MetThr: 1.53 ± 0.148
1.612MetVal: 1.612 ± 0.15
0.191MetTrp: 0.191 ± 0.051
0.792MetTyr: 0.792 ± 0.114
0.0MetXaa: 0.0 ± 0.0
Asn
3.046AsnAla: 3.046 ± 0.202
0.519AsnCys: 0.519 ± 0.087
3.456AsnAsp: 3.456 ± 0.208
4.084AsnGlu: 4.084 ± 0.234
2.404AsnPhe: 2.404 ± 0.17
4.48AsnGly: 4.48 ± 0.315
1.407AsnHis: 1.407 ± 0.146
3.961AsnIle: 3.961 ± 0.248
4.617AsnLys: 4.617 ± 0.257
4.89AsnLeu: 4.89 ± 0.267
1.257AsnMet: 1.257 ± 0.125
4.057AsnAsn: 4.057 ± 0.284
2.773AsnPro: 2.773 ± 0.195
2.39AsnGln: 2.39 ± 0.219
2.568AsnArg: 2.568 ± 0.192
4.193AsnSer: 4.193 ± 0.268
3.579AsnThr: 3.579 ± 0.224
4.357AsnVal: 4.357 ± 0.249
0.71AsnTrp: 0.71 ± 0.107
2.418AsnTyr: 2.418 ± 0.164
0.0AsnXaa: 0.0 ± 0.0
Pro
2.39ProAla: 2.39 ± 0.231
0.328ProCys: 0.328 ± 0.063
2.582ProAsp: 2.582 ± 0.21
2.8ProGlu: 2.8 ± 0.218
1.994ProPhe: 1.994 ± 0.144
2.322ProGly: 2.322 ± 0.188
0.601ProHis: 0.601 ± 0.103
2.827ProIle: 2.827 ± 0.206
2.431ProLys: 2.431 ± 0.176
2.623ProLeu: 2.623 ± 0.187
0.847ProMet: 0.847 ± 0.106
2.199ProAsn: 2.199 ± 0.153
0.847ProPro: 0.847 ± 0.114
0.929ProGln: 0.929 ± 0.11
1.325ProArg: 1.325 ± 0.116
2.404ProSer: 2.404 ± 0.191
2.472ProThr: 2.472 ± 0.209
3.36ProVal: 3.36 ± 0.288
0.41ProTrp: 0.41 ± 0.071
1.543ProTyr: 1.543 ± 0.151
0.0ProXaa: 0.0 ± 0.0
Gln
2.308GlnAla: 2.308 ± 0.166
0.219GlnCys: 0.219 ± 0.054
1.666GlnAsp: 1.666 ± 0.153
2.663GlnGlu: 2.663 ± 0.17
1.748GlnPhe: 1.748 ± 0.153
2.185GlnGly: 2.185 ± 0.193
0.806GlnHis: 0.806 ± 0.113
2.158GlnIle: 2.158 ± 0.168
1.68GlnLys: 1.68 ± 0.149
3.906GlnLeu: 3.906 ± 0.268
0.983GlnMet: 0.983 ± 0.122
1.571GlnAsn: 1.571 ± 0.15
1.024GlnPro: 1.024 ± 0.118
1.407GlnGln: 1.407 ± 0.138
1.352GlnArg: 1.352 ± 0.134
2.103GlnSer: 2.103 ± 0.166
2.008GlnThr: 2.008 ± 0.172
2.554GlnVal: 2.554 ± 0.186
0.26GlnTrp: 0.26 ± 0.059
1.735GlnTyr: 1.735 ± 0.157
0.0GlnXaa: 0.0 ± 0.0
Arg
2.418ArgAla: 2.418 ± 0.189
0.437ArgCys: 0.437 ± 0.084
2.554ArgAsp: 2.554 ± 0.177
2.718ArgGlu: 2.718 ± 0.215
2.267ArgPhe: 2.267 ± 0.201
2.445ArgGly: 2.445 ± 0.189
0.983ArgHis: 0.983 ± 0.114
2.868ArgIle: 2.868 ± 0.205
2.991ArgLys: 2.991 ± 0.197
3.811ArgLeu: 3.811 ± 0.259
1.325ArgMet: 1.325 ± 0.128
2.336ArgAsn: 2.336 ± 0.161
1.161ArgPro: 1.161 ± 0.156
1.257ArgGln: 1.257 ± 0.123
1.762ArgArg: 1.762 ± 0.153
2.978ArgSer: 2.978 ± 0.191
2.117ArgThr: 2.117 ± 0.179
3.155ArgVal: 3.155 ± 0.237
0.451ArgTrp: 0.451 ± 0.084
2.035ArgTyr: 2.035 ± 0.156
0.0ArgXaa: 0.0 ± 0.0
Ser
3.264SerAla: 3.264 ± 0.265
0.792SerCys: 0.792 ± 0.097
4.166SerAsp: 4.166 ± 0.246
3.934SerGlu: 3.934 ± 0.236
3.592SerPhe: 3.592 ± 0.22
4.453SerGly: 4.453 ± 0.259
1.175SerHis: 1.175 ± 0.114
4.712SerIle: 4.712 ± 0.26
4.589SerLys: 4.589 ± 0.245
6.324SerLeu: 6.324 ± 0.324
1.543SerMet: 1.543 ± 0.133
3.947SerAsn: 3.947 ± 0.246
2.486SerPro: 2.486 ± 0.183
1.735SerGln: 1.735 ± 0.158
2.595SerArg: 2.595 ± 0.212
4.466SerSer: 4.466 ± 0.258
3.688SerThr: 3.688 ± 0.228
5.054SerVal: 5.054 ± 0.295
0.833SerTrp: 0.833 ± 0.079
2.677SerTyr: 2.677 ± 0.222
0.0SerXaa: 0.0 ± 0.0
Thr
3.346ThrAla: 3.346 ± 0.236
0.615ThrCys: 0.615 ± 0.092
3.497ThrAsp: 3.497 ± 0.202
3.415ThrGlu: 3.415 ± 0.224
2.868ThrPhe: 2.868 ± 0.193
4.412ThrGly: 4.412 ± 0.27
1.065ThrHis: 1.065 ± 0.124
4.098ThrIle: 4.098 ± 0.245
3.756ThrLys: 3.756 ± 0.228
5.423ThrLeu: 5.423 ± 0.272
1.229ThrMet: 1.229 ± 0.129
3.21ThrAsn: 3.21 ± 0.211
2.964ThrPro: 2.964 ± 0.193
1.899ThrGln: 1.899 ± 0.18
2.527ThrArg: 2.527 ± 0.201
3.647ThrSer: 3.647 ± 0.238
3.715ThrThr: 3.715 ± 0.26
4.876ThrVal: 4.876 ± 0.343
0.642ThrTrp: 0.642 ± 0.093
2.09ThrTyr: 2.09 ± 0.163
0.0ThrXaa: 0.0 ± 0.0
Val
3.606ValAla: 3.606 ± 0.278
1.011ValCys: 1.011 ± 0.124
4.043ValAsp: 4.043 ± 0.206
5.163ValGlu: 5.163 ± 0.275
2.896ValPhe: 2.896 ± 0.208
4.166ValGly: 4.166 ± 0.235
1.257ValHis: 1.257 ± 0.141
5.177ValIle: 5.177 ± 0.251
5.805ValLys: 5.805 ± 0.29
5.627ValLeu: 5.627 ± 0.317
1.885ValMet: 1.885 ± 0.151
5.081ValAsn: 5.081 ± 0.297
2.745ValPro: 2.745 ± 0.182
2.527ValGln: 2.527 ± 0.208
2.773ValArg: 2.773 ± 0.179
5.231ValSer: 5.231 ± 0.279
4.671ValThr: 4.671 ± 0.307
4.808ValVal: 4.808 ± 0.306
0.615ValTrp: 0.615 ± 0.089
2.841ValTyr: 2.841 ± 0.208
0.0ValXaa: 0.0 ± 0.0
Trp
0.355TrpAla: 0.355 ± 0.07
0.191TrpCys: 0.191 ± 0.05
0.888TrpAsp: 0.888 ± 0.101
0.642TrpGlu: 0.642 ± 0.083
0.505TrpPhe: 0.505 ± 0.076
0.478TrpGly: 0.478 ± 0.079
0.191TrpHis: 0.191 ± 0.056
0.833TrpIle: 0.833 ± 0.095
0.861TrpLys: 0.861 ± 0.111
0.874TrpLeu: 0.874 ± 0.1
0.232TrpMet: 0.232 ± 0.051
0.724TrpAsn: 0.724 ± 0.113
0.178TrpPro: 0.178 ± 0.057
0.219TrpGln: 0.219 ± 0.056
0.492TrpArg: 0.492 ± 0.067
0.628TrpSer: 0.628 ± 0.09
0.587TrpThr: 0.587 ± 0.093
1.024TrpVal: 1.024 ± 0.111
0.096TrpTrp: 0.096 ± 0.037
0.615TrpTyr: 0.615 ± 0.091
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.83TyrAla: 1.83 ± 0.179
0.601TyrCys: 0.601 ± 0.075
2.349TyrAsp: 2.349 ± 0.194
2.035TyrGlu: 2.035 ± 0.149
2.063TyrPhe: 2.063 ± 0.165
2.841TyrGly: 2.841 ± 0.189
0.833TyrHis: 0.833 ± 0.126
2.677TyrIle: 2.677 ± 0.188
2.95TyrLys: 2.95 ± 0.229
3.879TyrLeu: 3.879 ± 0.243
0.929TyrMet: 0.929 ± 0.111
2.527TyrAsn: 2.527 ± 0.193
1.994TyrPro: 1.994 ± 0.179
2.063TyrGln: 2.063 ± 0.17
1.94TyrArg: 1.94 ± 0.152
2.609TyrSer: 2.609 ± 0.202
2.527TyrThr: 2.527 ± 0.187
2.623TyrVal: 2.623 ± 0.196
0.546TyrTrp: 0.546 ± 0.098
1.762TyrTyr: 1.762 ± 0.172
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 247 proteins (73213 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski